Disease relevance of RAB11FIP1

• Furthermore, significantly higher (P < 0.05) levels of RCP transcripts were detected during the peri-implantation phase in women with unexplained infertility (n=3) than in fertile women (n=3) [1].
• Sp1/retinoblastoma control protein (RCP) DNA binding increased within 30 min and peaked at 2-4 h after IR (450-600 cGy) in confluent radioresistant human malignant melanoma (U1-Mel) cells [2].
• These findings suggest that measurement of circulatory RCP and the immunohistochemical staining pattern of RCP in biopsy specimens could be exploited as an additional marker in diagnosis/prognosis of breast cancer in women [3].
• Because other vitamin carrier proteins (VCP) have been shown to be overexpressed in patients with malignant disease, we evaluated serum RCP levels in patients with adenocarcinoma of the breast [4].
• The pregnancies of two patients with mild intrahepatic cholestasis of pregnancy (RCP) were followed with detailed analyses of bile acids in urine [5].

High impact information on RAB11FIP1

• The peak lod scores in two of the pedigrees have dropped several lod units to clearly negative values at the RCP-F8-G6PD gene cluster [6].
• A similar effect on virus replication was observed when a carboxyl-terminal fragment of another ARE-associated protein, the Rab11 family interacting protein 1, was expressed in Madin-Darby canine kidney cells [7].
• Increased phosphorylation of Sp1 directly corresponded to Sp1/RCP binding and immediate-early gene induction, whereas pRb remained hypophosphorylated [2].
• Transfection of U1-Mel cells with the human papillomavirus E7 gene abrogated Sp1/RCP induction and G(0)/G(1) cell cycle checkpoint arrest responses, increased apoptosis and radiosensitivity, and augmented genetic instability (i.e., increased polyploidy cells) after IR [2].
• We therefore propose that RCP functions primarily as a Rab11 binding protein that regulates protein sorting in tubular endosomes [8].

Chemical compound and disease context of RAB11FIP1

• Using moxestrol, an analogue of estrogen and transient transfection of deletion and mutation containing RCP promoter/reporter constructs in chicken hepatoma (LMH2A) cells, we identified an estrogen response unit (ERU) composed of two consensus ERE 1/2 sites and one non-consensus ERE 1/2 site [9].
• Totally asymptomatic controls were characterized by a lack of anterior open bite, small symmetric RCP-ICP slides (greater than 0 less than 1 mm), and bilateral occlusal contact in RCP [10].

Biological context of RAB11FIP1

• The RCP-Rab11 complex regulates endocytic protein sorting [8].
• Interestingly, we found that RCP is associated as discrete patches or protein domains to early phagosomal membranes [11].
• The amino acid sequence of the Rab11-FIP proteins predicts coiled-coil formation in the conserved C-terminal domain [12].
• We have identified eight alternatively spliced Rab11-FIP1 gene transcripts from human chromosome 8 [13].
• The Rb gene does not appear to encode the RCPs as the expression of Rb in vivo does not correlate with RCE-RCP complex formation in vitro [14].

Anatomical context of RAB11FIP1

• RCP is predominantly membrane-bound and is expressed in all cell lines and tissues tested [15].
• Rab coupling protein associates with phagosomes and regulates recycling from the phagosomal compartment [11].
• In macrophages, RCP is predominantly membrane-bound and displays a punctuate vesicular pattern throughout the cytoplasm [11].
• This translocation is dependent on the presence of the C2 domain, because class I Rab11-FIP green-fluorescent-protein fusions that lack the C2 domain cannot translocate to the plasma membrane [16].
• Rab coupling protein (RCP): a novel target of progesterone action in primate endometrium [1].

Associations of RAB11FIP1 with chemical compounds

• In situ hybridization studies using digoxigenin-labeled DD2 revealed significantly higher (P < 0.05) localization of endometrial RCP transcripts in the proliferative phase than in the peri-implantation phase in control animals [1].
• In this study we present the comparative molecular analysis of bacterial communities of the aquatic plant Lemna minor from a contaminated site (RCP) and from a laboratory culture (EPA), as well as each of these with the addition of cadmium [17].
• Finally, the P2S2-5-Ava-BBN[7-14]NH2 bombesin (BBN) analogue was synthesized, the PH2 groups converted to P(CH2OH)2 groups and subsequently labeled with 188Re to yield a 188Re-labeled bombesin analogue with a RCP of >/=90% [18].
• Interestingly, RCP promoter is regulated by moxestrol in estrogen responsive human MCF-7 cells, but not in other cell lines such as NIH3T3 and HepG2 despite estrogen receptor-alpha (ER-alpha) co transfection [9].
• RESULTS: In both the RCP and the Triclosan group, gingival health improved significantly between baseline and the following examinations [19].

Physical interactions of RAB11FIP1

• The Rab11 family of interacting proteins (Rab11-FIP) is a recently identified protein family composed of, to date, six members that interact with Rab11 [16].
• However, Rab11-FIP4 does not appear to interact directly with Rab coupling protein (RCP) [20].
• To date, five such proteins have been described in humans, all of which interact with Rab11, and one (RCP) also interacts with Rab4 [21].

Regulatory relationships of RAB11FIP1

• However, transferrin trafficking was not significantly altered in HeLa cells over-expressing expressing any of the EGFP-Rab11-FIP1 proteins [13].

Other interactions of RAB11FIP1

• The binding of Rab11-FIP1, Rab11-FIP2, and Rab11-FIP3 to Rab11a was dependent upon a conserved carboxyl-terminal amphipathic alpha-helix [22].
• RCP is a homologue of Rip11 and therefore belongs to the recently described Rab11-FIP family [15].
• Furthermore, our data indicate that the RCP-Rab11 complex regulates the sorting of transferrin receptors from the degradative to the recycling pathway [8].
• RESULTS: We observed amplification of 8p12 (amplified at RAB11FIP1 and/or FGFR1) in 22.8%, 8q24 in 6.1%, 11q13 in 19.6%, 12p13 in 4.1%, 17q12 in 9.9%, 20q13Z (amplified at ZNF217 only) in 9.9%, and 20q13Co (co-amplification of two or three 20q13 loci) in 8.5% of cases [23].
• We have recently identified Rab11-FIP4 as the sixth member of the Rab11-FIP family of Rab11 interacting proteins [20].

Analytical, diagnostic and therapeutic context of RAB11FIP1

• We used fluorescence confocal microscopy and biochemical approaches to evaluate the participation of RCP during particle uptake and phagosome maturation [11].
• To confirm the identity of DD2 as RCP, RT-PCR studies were carried out with a forward primer deduced from the RCP sequence and a reverse primer from the DD2 sequence [1].
• Characterisation of the Rab binding properties of Rab coupling protein (RCP) by site-directed mutagenesis [24].
• Long-range sequence analysis in Xq28: thirteen known and six candidate genes in 219.4 kb of high GC DNA between the RCP/GCP and G6PD loci [25].
• The presence of RCP and its involvement in RF internalization was analyzed by immunofluorescence and immunobinding assays using chicken RCP (cRCP) antibodies [26].

References