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Gene Review

MAGI1  -  membrane associated guanylate kinase, WW...

Homo sapiens

Synonyms: AIP-3, AIP3, Atrophin-1-interacting protein 3, BAI1-associated protein 1, BAIAP1, ...
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Disease relevance of MAGI1

  • Inhibition of HIV-1 replication was specific for X4 HIV-1 strains as demonstrated by MAGI assays [1].
  • Lamellarin alpha 20-sulfate was tested against wild type HIV using the MAGI indicator cell assay and found to inhibit early steps of HIV replication [2].
  • Peptide T acted to block viral entry as it inhibited in the MAGI cell assay and blocked infection in the luciferase reporter assay using HIV virions pseudotyped with ADA envelope [3].
  • An MTT assay using CD4-, CXCR4-, and CCR5-transduced human glioma NP-2 cells (NCK45 cells) was established and then compared with the representative assays including multinuclear activation of a galactosidase indicator assay (MAGI assay) [4].
  • Infertile men were divided into four groups as follows: (1) varicocele (n=23), (2) 3 months after varicocelectomy (post-varicocele, n=14), (3) male accessory gland infection (MAGI, n=10) and (4) bilateral testicular atrophy (n=8) [5].

High impact information on MAGI1


Biological context of MAGI1


Anatomical context of MAGI1

  • MAGI-1: a widely expressed, alternatively spliced tight junction protein [13].
  • A broader examination of MAGI-1 expression in vivo shows that it is present in the tight junctions of all epithelial cell types examined [13].
  • By immunocytochemical analysis of COS-7 cells transfected with BAI1 and BAP1, both products were co-localized at the cytoplasmic membrane, especially at cell-cell junctions [8].
  • The association of megalin with MAGI-1 may allow the assembly of a multiprotein complex, in which megalin may serve a nonendocytic function in glomerular podocytes [9].
  • Western analysis of endogenous MAGI-1 from glomerular preparations suggests that it is associated with the cytoskeleton and seems to be expressed in a different form, compared with cell line-derived endogenous MAGI-1 [9].

Associations of MAGI1 with chemical compounds

  • BAP1 is a novel member of the MAGUK (membrane-associated guanylate kinase homologue) family; it possesses a guanylate kinase domain, WW domains, and multiple PDZ domains [8].
  • Because of its interaction with MAGI-1 and its localization to cell-cell junctions, this novel protein has been given the name MAGI-1-associated coiled-coil tight junction protein (MASCOT) [14].
  • (13)C, (15)N and (1)H Resonance Assignment of the PDZ1 domain of MAGI-1 using QUASI [15].
  • Biochemical studies reveal that nephrin directly binds to the middle PDZ domains of MAGI-1 through its carboxyl terminus but does not bind to ZO-1 [16].
  • It is concluded that features of MAGI in semen may regress spontaneously and are not influenced by the doxycycline treatment [17].

Physical interactions of MAGI1

  • RESULTS: MAGI-1/BAP1 similarly interacted with Rap GEP in cell-free and intact cell systems [18].

Other interactions of MAGI1


Analytical, diagnostic and therapeutic context of MAGI1

  • Sequence analysis of overlapping cDNAs revealed the existence of three splice variants that are predicted to encode MAGI-1 proteins with different COOH termini [20].
  • Here we show that stable expression of simian TRIM5alpha(rh) via a lentiviral vector in a permissive cell culture line, Magi-CXCR4, conferred resistance to HIV-1 [21].
  • Contrary to SupT1 cells, which encode several mutations on the p53 DNA binding domain, death of HIV-1-induced syncytia was reduced in cocultures of HeLa P4-R5 MAGI with persistently infected HIV-1 cells [22].
  • Immunoelectron microscopy shows that the localization of MAGI-1 is restricted to the slit diaphragm, whereas JAM4 is also distributed on apical membranes of podocytes [16].


  1. Inhibition of HIV-1 infection by down-regulation of the CXCR4 co-receptor using an intracellular single chain variable fragment against CXCR4. BouHamdan, M., Strayer, D.S., Wei, D., Mukhtar, M., Duan, L.X., Hoxie, J., Pomerantz, R.J. Gene Ther. (2001) [Pubmed]
  2. Lamellarin alpha 20-sulfate, an inhibitor of HIV-1 integrase active against HIV-1 virus in cell culture. Reddy, M.V., Rao, M.R., Rhodes, D., Hansen, M.S., Rubins, K., Bushman, F.D., Venkateswarlu, Y., Faulkner, D.J. J. Med. Chem. (1999) [Pubmed]
  3. Peptide T inhibits HIV-1 infection mediated by the chemokine receptor-5 (CCR5). Ruff, M.R., Melendez-Guerrero, L.M., Yang, Q.E., Ho, W.Z., Mikovits, J.W., Pert, C.B., Ruscetti, F.A. Antiviral Res. (2001) [Pubmed]
  4. A novel colorimetric assay for CXCR4 and CCR5 tropic human immunodeficiency viruses. Kajiwara, K., Kodama, E., Matsuoka, M. Antivir. Chem. Chemother. (2006) [Pubmed]
  5. Relationship between seminal plasma interleukin-6 and tumor necrosis factor alpha levels with semen parameters in fertile and infertile men. Koçak, I., Yenisey, C., Dündar, M., Okyay, P., Serter, M. Urol. Res. (2002) [Pubmed]
  6. MAGI-1 is required for Rap1 activation upon cell-cell contact and for enhancement of vascular endothelial cadherin-mediated cell adhesion. Sakurai, A., Fukuhara, S., Yamagishi, A., Sako, K., Kamioka, Y., Masuda, M., Nakaoka, Y., Mochizuki, N. Mol. Biol. Cell (2006) [Pubmed]
  7. Interaction of two actin-binding proteins, synaptopodin and alpha-actinin-4, with the tight junction protein MAGI-1. Patrie, K.M., Drescher, A.J., Welihinda, A., Mundel, P., Margolis, B. J. Biol. Chem. (2002) [Pubmed]
  8. Cloning and characterization of BAI-associated protein 1: a PDZ domain-containing protein that interacts with BAI1. Shiratsuchi, T., Futamura, M., Oda, K., Nishimori, H., Nakamura, Y., Tokino, T. Biochem. Biophys. Res. Commun. (1998) [Pubmed]
  9. The membrane-associated guanylate kinase protein MAGI-1 binds megalin and is present in glomerular podocytes. Patrie, K.M., Drescher, A.J., Goyal, M., Wiggins, R.C., Margolis, B. J. Am. Soc. Nephrol. (2001) [Pubmed]
  10. Mutation of the dominant endocytosis motif in human immunodeficiency virus type 1 gp41 can complement matrix mutations without increasing Env incorporation. West, J.T., Weldon, S.K., Wyss, S., Lin, X., Yu, Q., Thali, M., Hunter, E. J. Virol. (2002) [Pubmed]
  11. Ocular pathology in The Parable of the Blind Leading the Blind and other paintings by Pieter Bruegel. Karcioglu, Z.A. Survey of ophthalmology. (2002) [Pubmed]
  12. Highly specific monoclonal antibody demonstrates that pregnancy-specific glycoprotein (PSG) is limited to syncytiotrophoblast in human early and term placenta. Zhou, G.Q., Baranov, V., Zimmermann, W., Grunert, F., Erhard, B., Mincheva-Nilsson, L., Hammarström, S., Thompson, J. Placenta (1997) [Pubmed]
  13. MAGI-1: a widely expressed, alternatively spliced tight junction protein. Laura, R.P., Ross, S., Koeppen, H., Lasky, L.A. Exp. Cell Res. (2002) [Pubmed]
  14. Identification and characterization of a novel tight junction-associated family of proteins that interacts with a WW domain of MAGI-1. Patrie, K.M. Biochim. Biophys. Acta (2005) [Pubmed]
  15. (13)C, (15)N and (1)H Resonance Assignment of the PDZ1 domain of MAGI-1 using QUASI. Charbonnier, S., Coutouly, M.A., Kieffer, B., Trav??, G., Atkinson, R.A. J. Biomol. NMR (2006) [Pubmed]
  16. MAGI-1 is a component of the glomerular slit diaphragm that is tightly associated with nephrin. Hirabayashi, S., Mori, H., Kansaku, A., Kurihara, H., Sakai, T., Shimizu, F., Kawachi, H., Hata, Y. Lab. Invest. (2005) [Pubmed]
  17. The effect of doxycycline in infertile couples with male accessory gland infection: a double blind prospective study. Comhaire, F.H., Rowe, P.J., Farley, T.M. Int. J. Androl. (1986) [Pubmed]
  18. Membrane-associated guanylate kinase with inverted orientation (MAGI)-1/brain angiogenesis inhibitor 1-associated protein (BAP1) as a scaffolding molecule for Rap small G protein GDP/GTP exchange protein at tight junctions. Mino, A., Ohtsuka, T., Inoue, E., Takai, Y. Genes Cells (2000) [Pubmed]
  19. HPV E6 specifically targets different cellular pools of its PDZ domain-containing tumour suppressor substrates for proteasome-mediated degradation. Massimi, P., Gammoh, N., Thomas, M., Banks, L. Oncogene (2004) [Pubmed]
  20. MAGI-1, a membrane-associated guanylate kinase with a unique arrangement of protein-protein interaction domains. Dobrosotskaya, I., Guy, R.K., James, G.L. J. Biol. Chem. (1997) [Pubmed]
  21. TRIM5alpharh expression restricts HIV-1 infection in lentiviral vector-transduced CD34+-cell-derived macrophages. Anderson, J., Akkina, R. Mol. Ther. (2005) [Pubmed]
  22. Inhibition of HIV-1 replication by RNA interference of p53 expression. Pauls, E., Senserrich, J., Clotet, B., Esté, J.A. J. Leukoc. Biol. (2006) [Pubmed]
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