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Chemical Compound Review

geneticin     (2R,3S,4R,5R,6R)-5-amino-6- [(1R,2R,3S,4R...

Synonyms: G 418 sulfate, CHEMBL215226, SureCN5006573, KST-1A8755, G-418, ...
 
 
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Disease relevance of geneticin

 

High impact information on geneticin

 

Biological context of geneticin

  • Transformants were first selected for resistance to the antibiotic G418 conferred by the neo gene from Tn5 carried by the same plasmid [11].
  • This plasmid has a selectable marker gene conferring resistance to the antibiotic G418 and carries a 13-kilobase region of the Kluyveromyces lactis genome including LAC4, a beta-galactosidase gene [12].
  • Both diploid and immortal p-null melanocytes grew more slowly than wild-type melanocytes, however, and were unusually susceptible to the antibiotic G418; these abnormalities were corrected by culture in high concentrations of L-tyrosine [13].
  • Using DNA transfection, the human chromosome was tagged with a selectable marker (the pSV2neo gene, which encodes resistance to the antibiotic G418), transferred to mouse A9 cells by cell hybridization and microcell transfer techniques, and then transferred to SiHa cells by microcell transfer [14].
  • Plasmid pNF2 also contains the kanamycin-resistance (kanR) gene derived from Tn903 and confers resistance in yeast to the antibiotic G418. pNF2 was converted into an integrating vector (pNF3) by deleting a 2.2-kb EcoRI fragment containing a sequence that determines autonomous replication in yeast [15].
 

Anatomical context of geneticin

  • CHO cells were cotransfected with murine genomic DNA fragments from F9 cells and plasmid DNA containing a resistance gene to the antibiotic G418 [16].
  • In order to investigate its function, we have established lines of mouse fibroblasts stably expressing the b-protein by co-transfection of a b-protein expression vector and a plasmid conferring resistance to the antibiotic G418 [17].
  • HeLa cells which were transfected with this vector and selected for resistance to the antibiotic G418 maintained the construct primarily in episomal form during more than one year of continuous culture, with little or no integration into the host genome [18].
  • Epithelial cells were derived from a defined location of rabbit kidney, the thick ascending limb of Henle's loop, and were co-transfected with genes from the early region of SV40 together with pSV2-neo DNA (which confers resistance to the antibiotic G418) [19].
  • The proviral sequences were rescued from antibiotic G418-resistant virus-infected cells after fusion with Cos cells, amplified as plasmids in Escherichia coli and analysed [20].
 

Associations of geneticin with other chemical compounds

  • This work also demonstrates that the kanamycin resistance gene carried by the bacterial transposon Tn903 can be expressed in S. pombe, as shown by its ability to inactivate the antibiotic G418 [21].
  • To determine if overexpression of manganese-containing SOD (MnSOD) alters cell sensitivity to asbestos, an expression cassette containing murine MnSOD cDNA was cotransfected with pSV2neo, a plasmid conferring resistance to the antibiotic G418, into a diploid cell line of hamster tracheal epithelial (HTE) cells [22].
  • METHODS: ECs harvested from external jugular veins were grown in culture, with 80% of the cells from each culture transduced by incubation with an LXSN-type retroviral vector carrying a gene for human prourokinase and a neomycin resistance gene for selection in antibiotic G418 [23].
  • A codon optimized Blo t 5 gene was synthesized by PCR and the codon optimized or wild-type Blo t 5 gene in pcDNA3.0 was transfected into CHO-K1 cells and stably selected with Geneticin (G418) [24].
  • Sf9 cell lines expressing AcMNPV P35 or an epitope-tagged P35 protein were generated using a double selection technique, involving selection in the antibiotic G418, followed by a second round of selection by exposure to actinomycin D, a potent inducer of apoptosis in Sf9 cells [25].
 

Gene context of geneticin

 

Analytical, diagnostic and therapeutic context of geneticin

  • Transfectants expressing both the human MER-2 gene and G418 resistance were isolated by selection in the antibiotic G418, followed by indirect immunofluorescence using the monoclonal antibody 1D12, which recognizes the MER-2 antigen, manual enrichment, and single-cell cloning [30].
  • Three treatment groups were evaluated: G-I, transduced CMVEC, selected in antibiotic G418 for 10 days (n=5); G-II, CMVEC selected in G418 and recovered from selection for 4 days (n=5); and G-III, control group of naive CMVEC (n=6) [31].

References

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  4. Construction and properties of a cell line constitutively expressing the herpes simplex virus glycoprotein B dependent on functional alpha 4 protein synthesis. Arsenakis, M., Hubenthal-Voss, J., Campadelli-Fiume, G., Pereira, L., Roizman, B. J. Virol. (1986) [Pubmed]
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  8. Genetically engineered smooth muscle cells as linings to improve the biocompatibility of cardiovascular prostheses. Scott-Burden, T., Tock, C.L., Schwarz, J.J., Casscells, S.W., Engler, D.A. Circulation (1996) [Pubmed]
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  11. Genetic complementation of UV-induced DNA repair in Chinese hamster ovary cells by the denV gene of phage T4. Valerie, K., de Riel, J.K., Henderson, E.E. Proc. Natl. Acad. Sci. U.S.A. (1985) [Pubmed]
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  14. Normal human chromosome 11 suppresses tumorigenicity of human cervical tumor cell line SiHa. Koi, M., Morita, H., Yamada, H., Satoh, H., Barrett, J.C., Oshimura, M. Mol. Carcinog. (1989) [Pubmed]
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  17. The mouse brown (b) locus protein has dopachrome tautomerase activity and is located in lysosomes in transfected fibroblasts. Winder, A.J., Wittbjer, A., Rosengren, E., Rorsman, H. J. Cell. Sci. (1993) [Pubmed]
  18. A novel BK virus-based episomal vector for expression of foreign genes in mammalian cells. De Benedetti, A., Rhoads, R.E. Nucleic Acids Res. (1991) [Pubmed]
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  21. High-frequency cotransformation by copolymerization of plasmids in the fission yeast Schizosaccharomyces pombe. Sakai, K., Sakaguchi, J., Yamamoto, M. Mol. Cell. Biol. (1984) [Pubmed]
  22. Transfection of a manganese-containing superoxide dismutase gene into hamster tracheal epithelial cells ameliorates asbestos-mediated cytotoxicity. Mossman, B.T., Surinrut, P., Brinton, B.T., Marsh, J.P., Heintz, N.H., Lindau-Shepard, B., Shaffer, J.B. Free Radic. Biol. Med. (1996) [Pubmed]
  23. Improved adherence of genetically modified endothelial cells to small-diameter expanded polytetrafluoroethylene grafts in a canine model. Falk, J., Townsend, L.E., Vogel, L.M., Boyer, M., Olt, S., Wease, G.L., Trevor, K.T., Seymour, M., Glover, J.L., Bendick, P.J. J. Vasc. Surg. (1998) [Pubmed]
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