Disease relevance of picric acid

• Of the sections fixed with Bouin's solution, the staining reaction was drastically reduced in the normal bladder and the staining was totally negative in the regenerative hyperplasia caused by freezing [1].
• Our data indicate a decrease in radioimmunoassayable LHRH content of brain fixed in Bouin's solution and a further significant reduction following dehydration with alcohol [2].
• Comparative genomic hybridization study of paraffin-embedded dedifferentiated liposarcoma fixed with Holland Bouin's fluid [3].
• Using a modified indirect immunofluorescent (IF) technique in which cryostat tissue sections were fixed in Bouin's solution for ten minutes prior to reaction with sera under test, we have looked for antibodies to the hepatocyte membrane (HMA) in the sera of patients with chronic active hepatitis (CAH) and primary biliary cirrhosis (PBC) [4].
• The animals were sacrificed on day 12, their lungs infused with Bouin's solution, and lung metastases counted [5].

High impact information on picric acid

• Mammary gland biopsies and whole glands were fixed in Bouin's fluid and embedded in paraffin [6].
• Fixation in Bouin's solution for 2 hours gave in situ hybridization results comparable with buffered formalin fixation but with poorer cytologic detail [7].
• Tissue was fixed in Bouin's fluid, and the percentage of BrdU-labeled Sertoli cells at each developmental stage was calculated [8].
• Pineal glands selected from cases with postmortem intervals of one to 24 hours were fixed in Bouin's fluid and immunostained using the peroxidase-antiperoxidase technique and an antiserum against human glial fibrillary acidic (GFA) protein [9].
• Portions of the tissue were fixed in Bouin's solution for immunocytochemistry, and the remainder was subdivided into functionalis, basalis, and myometrium and subjected to organ explant culture [10].

Chemical compound and disease context of picric acid

• As far as embryonal rhabdomyosarcomas are concerned the electron microscopic study is possible, and it gives useful results even when material fixed with routine histologic methods (formalin, Bouin's solution) are used [11].

Biological context of picric acid

• In the present study we wish to report the results of immunocytochemical localization of various proteins in testis fixed by perfusion with Bouin's fluid and embedded in polyester wax, a ribboning embedding medium for histology [12].
• Chicken ovaries were obtained 2 h after oviposition and fixed with Bouin's solution [13].
• Whole fetuses (13.5 and 14.0 postcoitum (p.c.), testes, and ovaries dissected from fetuses on Days 14.5 p.c. to 20.5 p.c. and testes obtained on Days 3, 5, and 7 postpartum were fixed in Bouin's solution and processed for immunocytochemistry [14].
• Tissues from rats between day 5 and day 8 of gestation and mice between day 5 and day 7 of gestation were fixed in Bouin's solution and embedded in wax prior to lectin histochemistry [15].
• Tadpoles ranging from stage 12 to 57 (Nieuwkoop and Faber 1956) were fixed in Zamboni's or Bouin's solution [16].

Anatomical context of picric acid

• An immunocytochemical technique has been developed for the identification in situ of immunoglobulin-producing cells in tissues fixed in Bouin's solution and embedded in paraffin [17].
• On cryostat sections of fresh-frozen mammary tissue post-fixed in acetone, or on paraffin sections also from mammary tissue fixed in Bouin's solution, the MAb reacted preferentially with glandular epithelial cells and staining was restricted to the nuclei [18].
• Fetuses were individually weighed, and two-thirds of them were fixed in Bouin's solution and examined for soft-tissue anomalies [19].
• On Day 11, monkeys were castrated, and one-half of a testis was fixed in Bouin's fluid [20].
• Serial sections (8-10 micron-thick) prepared from corpora lutea that had been fixed in Bouin's solution and embedded in paraffin were immunostained for neurophysin or oxytocin using the peroxidase-antiperoxidase (PAP) procedure [21].

Associations of picric acid with other chemical compounds

• Specific gravities of human pineals fixed in Bouin's fluid, Bouin's fluid followed by 80% ethanol, 80% ethanol, and embalming fluid were found to be 1.113 +/- 0.025 (N = 5), 0.941 +/- 0.009 (N = 5), 0.990 +/- 0.022 (N = 3), and 1.149 +/- 0.021 (N = 7), respectively [22].
• Fetuses fixed in Bouin's solution or fetal pancreas cells fixed in 4% paraformaldehyde were processed for routine histology and for immunohistology to detect hormone expression and bromodeoxyuridine (BrdU) uptake [23].
• Furthermore, a set of samples was fixed with Bouin's solution, embedded in paraffin and used for immunohistochemistry with a polyclonal antibody against connexin 45 as well as for in situ hybridization studies with digoxigenin labeled connexin 45 riboprobes [24].
• The aortas were placed in gluteraldehyde for subsequent transmission electron microscopy, Bouin's solution for TUNEL, or paraformaldehyde for immunohistochemical staining for caspase-9, caspase-3, and Bid [25].
• The tooth germs were fixed in Bouin's solution, embedded in paraffin, sectioned and stained with haematoxylin and eosin [26].

Gene context of picric acid

• The tissue was fixed in Bouin's solution and embedded in paraffin for immunocytochemistry using polyclonal antibodies against EGF and EGF-R and a monoclonal antibody to TGF alpha [27].
• However, microwave denaturation of these tissues preserved MAP2 immunoreactivity better than fixation with Bouin's solution or formalin [28].
• With regard to the potential adverse effect of the fixatives used, PCR products were obtained in 100%, 91.5%, 90.5%, and 0% of tumors fixed with AFA, buffered formalin, Holland Bouin, and conventional Bouin's fluid, respectively [29].
• Keratin polypeptides could not be adequately demonstrated in extracts prepared from formalin- or Bouin's solution-fixed tissues [30].
• Sixteen liposarcoma tumors both frozen and fixed in Holland Bouin's fluid were characterized by CGH [3].

Analytical, diagnostic and therapeutic context of picric acid

• Tissues obtained at laparotomy were fixed in Bouin's solution and embedded in paraffin; immunoreactive oxytocin was localized with peroxidase-antiperoxidase and 3.3' diaminobenzidine [31].
• Sera were examined by indirect immunofluorescence on rat liver sections previously fixed in Bouin's fluid [32].
• In frozen sections and in tissues fixed with either Bouin's solution, chloroform-methanol, or buffered paraformaldehyde-glutaraldehyde increased cytoplasmic background staining and loss of granule immunoreactivity were observed [33].
• A total of 287 testes biopsies were performed in the office under local anesthesia, using a modified needle and technique to collect the tissue which was then fixed in Bouin's solution [34].
• The importance of tissue fixation for light microscopic immunohistochemical localization of peroxisomal proteins: the superiority of Carnoy's fixative over Baker's formalin and Bouin's solution [35].

References