Disease relevance of NSC322921

• Living ganglion cells of the six species mentioned above were treated with quinacrine and H 33258 [1].
• A rapid and sensitive method is described for the determination of parasitemia in Plasmodium falciparum cultures using the fluorescence activated cell sorter and DNA-binding fluorochrome, 33258 Hoechst [2].
• The approach was to selectively incorporate the free base analogue 5-bromouracil (5-BrUra) into the mycoplasmas followed by photosensitization of the DNA containing 5-BrUra by low concentrations of the fluorochrome 33258-Hoechst [3].
• The unusually high A + T content in mycoplasma DNA makes these organisms suitable candidates for the induction of breakage by the combined action of 5-BrUra, 33258-Hoechst and light because 33258-Hoechst has a high affinity for A + T base pairs and an even higher affinity for A-BrUra base pairs [3].
• Cell cycle kinetics was studied using the quenching of 33258 Hoechst fluorescence by bromodeoxyuridine incorporation to investigate effects of high pressure on the toxicity of a welding fume component--chromate [4].

High impact information on NSC322921

• A technique involving 5-bromodeoxyuridine, 33258 Hoechst, and fluorescence microscopy has been used to analyze replication kinetics in cells from embryonic and adult mice bearing the Cattanach [T(X;7)ICt] translocation in a balanced or an unbalanced form [5].
• The 24 human chromosome types of normal diploid fibroblast cell strain were classified into 15 groups by high-resolution flow cytometry on the basis of 33258 Hoechst fluorescence [6].
• The number of previous cell replications that a metaphase cell has undergone in the presence of BrdUrd can be determined by the differential fluorescent patterns of metaphse chromosomes stained with Hoechst dye 33258 [7].
• Susceptibility of proliferating cytolytic T lymphocytes to 33258 Hoechst-modified BUdR and light treatment: quantitation of the residual precursor cells by limiting dilution analysis [8].
• The advantages of 33258 Hoechst relative to Feulgen compounds used previously to stain these structures include a greater intensity of fluorescence, the absence of fading or rapid quenching of the fluorescence, and the omission of acid hydrolysis from the procedure for removal of ribonucleic acid [9].

Biological context of NSC322921

• Both qualitative and quantitative differences in 33258 Hoechst fluorescence intensity, reflecting alterations in replication kinetics, can be detected between the two X chromosomes in female cells [10].
• Condensation--inhibition by 33258-Hoechst of centromeric heterochromatin in prematurely condensed mouse chromosomes [11].
• The pleiotropic effects of 33258-Hoechst on the cell cycle in Chinese hamster cells in vitro [12].
• Pattern of condensation of mouse and Chinese hamster chromosomes in G2 and mitosis of 33258-Hoechst-treated cells [13].
• Fluorescence analysis of late DNA replication in mouse metaphase chromosomes using BUdR and 33258 Hoechst [14].

Anatomical context of NSC322921

• Unlike other techniques, flow cytometric analysis of BrdU-quenched 33258 Hoechst fluorescence may be used to measure cell activation and the G1, S, and G2/M compartment distributions in each of three successive cell cycles after growth stimulation of human peripheral blood lymphocytes [15].
• Thiazole orange and Hoechst dye 33258 at pH 2 stain ribosomes bound to the ER, providing evidence that the cortical ER is rough ER [16].
• As observed by 33258 Hoechst staining and fluorescent microscopy, these sequences are consistently found to be located on the sides of the nuclei nearest to the vitelline membrane [17].
• Analogue substitution over a fraction of the DNA synthesis period results in distinctive 33258 Hoechst fluorescence staining of late replicating chromatin, detectable at spermatogonial or spermatocyte I and II stages [18].
• The difference in sensitivity of human chromosomes in different cell types to 33258-Hoechst probably reflects differences in the cell-membrane permeabilities to 33258-Hoechst [19].

Associations of NSC322921 with other chemical compounds

• Quinacrine induced a generalized lengthening and swelling of the chromosomes and H 33258 the decondensation of specific heterochromatic regions [1].
• The binding pattern of MAP2 to chromosomes is similar to that found for the binding of the bisbenzimidazole derivative 33258 Hoechst, which also binds preferentially to AT-rich regions [20].
• Mitochondrial DNA (mtDNA) from the cryptomonad Pyrenomonas salina was isolated by CsCl-buoyant density centrifugation of whole-cell DNA in the presence of Hoechst dye 33258. mtDNA consists of circular molecules about 47 kb in size as estimated from restriction enzyme analysis [21].
• The cells were treated with triton X-100 and the nucleic acid content of the extract was determined by measuring DNA fluorescence in the presence of Hoechst dye 33258 [22].
• Microfluorometric measurements were used to document the occurrence of energy transfer between quinacrine or 33258 Hoechst as energy donor and ethidium or 7-aminoactinomycin D as acceptor when used jointly to stain cytologic preparations of human metaphase chromosomes [23].

Gene context of NSC322921

• Human metaphase chromosomes, substituted with 5'-bromodeoxyuridine (BrdUrd) for one, two or three rounds of replication, were briefly pretreated with ultraviolet light (UV), in the presence of 33258 Hoechst, and subsequently digested with either exonuclease III or S1 nuclease [24].
• Condensation of the human Y chromosome, which contains a large heterochromatic region rich in A-T base pairs, is drastically inhibited by 33258-Hoechst treatment of fibroblasts and lymphoblasts [19].
• We studied the effectiveness of harmine as a substitute for 33258 Hoechst in the fluorescence-plus-Giemsa technique, using Allium cepa chromosomes after 5-bromo-2'-deoxyuridine (BrdU) incorporation [25].

Analytical, diagnostic and therapeutic context of NSC322921

• Total DNA was isolated from the parasitic protozoan Giardia lamblia and separated into two distinct populations of different densities by centrifugation through CsCl gradients containing Hoechst dye 33258 [26].
• Newly-replicated, unifilarly BUdR-substituted DNA was selectively degraded by treatment with 33258 Hoechst plus near UV light followed by S1 nuclease digestion; the resistant DNA was analyzed for its content of 18s and 28s rDNA or dihydrofolate reductase (DHFR) sequences via Southern blot analysis [27].
• Plasmodium-infected blood cells analyzed and sorted by flow fluorimetry with the deoxyribonucleic acid binding dye 33258 Hoechst [28].
• Absorption, fluroescence and circular dichroism measrements on 33258 Hoechst-deoxyribonucleic acid (DNA) complexes are consistent with the existence of two types of dye-binding interactions [29].
• Induction of high frequencies of endoreduplication in mammalian cell cultures with 33258 Hoechst and rubidazone [30].

References