Disease relevance of Pyrodin

• On the other hand, increases of ODC activity and spleen weight were observed in the spleen of mice with hemolytic anemia induced by acetylphenylhydrazine, but the extent of these increases were smaller than those in the spleen of mice infected with P. berghei [1].
• Hemolysis is obtained by administering 25 mg/liter of acetylphenylhydrazine in the breeding water for 36 h [2].
• Red blood cells (RBC) of patients affected by breast cancer are more sensitive to the denaturing action of acetylphenylhydrazine (APH), hence the formation time of hemin, one of the last oxidation products, is significantly shorter then in normal subjects [3].
• 2. Guinea-pigs are comparatively resistant to acetylphenylhydrazine, a drug used to induce reticulocytosis in rabbits [4].
• In vitro oxidative load of acetylphenylhydrazine proved the impaired glutathione stability of the erythrocytes in acute CD and cow's milk allergy [5].

High impact information on Pyrodin

• High-reticulocyte control RBC and sickle trait RBC behaved exactly like normal RBC, while HbCC RBC and RBC having membranes gilded with hemoglobin iron because of prior exposure to acetylphenylhydrazine showed an abnormal peroxidation response like that of sickle RBC [6].
• Newts (Triturus cristatus) made anemic with acetylphenylhydrazine (APH) fail to regenerate erythrocytes (RBC's) immediately and exhibit a latent period of 1.5-2 wk during which animals lack RBC's and are aplastic [7].
• Adult betaYAC/MBD2-/- mice continue to express the gamma-globin gene at a level commensurate with 5-azacytidine treatment, 10- to 20-fold over that observed with 1-acetyl-2-phenylhydrazine treatment alone [8].
• Moreover, exposure of intact RBC to 20 mmol/L APH induced depletion of procalpain activity for which the time course was inversely related to formation of Heinz bodies [9].
• Oxidative treatment of column-purified Hb with acetylphenylhydrazine produces an immediate 4-fold increase in its specific methyl-accepting activity coincident with the production of hemichrome forms known to possess a higher affinity for membrane binding sites [10].

Chemical compound and disease context of Pyrodin

• Xylitol mediated amelioration of acetylphenylhydrazine-induced hemolysis in rabbits [11].
• Hemolytic anemia was induced in rats by a single ip injection of 60 mg/kg of 20 mg/ml acetylphenylhydrazine (APH) in 20% (v/v) ethanol on the first experimental day (day 0) [12].

Biological context of Pyrodin

• The results show a two-fold increase in resistance to oxidative stress by parasites adapted to G6PD-Mediterranean deficient host cells as compared to unadapted ones, but the parasite-red cell system remains 4 times more sensitive to APH than normal infected cells [13].
• Repetitive injection of phenylhydrazine or acetylphenylhydrazine in rats resulted in gradually increasing levels of fluorescence despite the return in hematocrit toward normal [14].
• When 30 mM beta-acetylphenylhydrazine (APH) was applied to HK and LK cells, lipid peroxidation and hemoglobin denaturation occurred [15].
• In animals treated with acetylphenylhydrazine (hemolitic agent), the heart intervenes in the erythropoiesis also when hematic equilibrium is lightly altered [16].
• During incubation with APH, osmotic fragility increased markedly in LK cells, while HK cells showed very little change [15].

Anatomical context of Pyrodin

• These cells can be generated in increased numbers in sickle cell blood by incubating samples in 1.5 mM-acetylphenylhydrazine (APH) solution for two hours at 37 degrees C. Their formation is partially blocked by incubation with the reducing agent, dithiothreitol (DTT) [17].
• Effect of mercury, lead and acetylphenylhydrazine (APHZ) on erythrocyte and bone marrow glucose-6-phosphate dehydrogenase (E.C. 1.1.1.49). an electrophoretic study [18].
• A new 'tissue-stamp culture' method was developed for stamping proliferating erythroblasts of mouse spleens on collagen-coated coverslips after inducing haemolytic anaemia by administration of 1-acetyl-2-phenylhydrazine, and then adherent splenic cells were cultured for a few days [19].
• Effects of acetylphenylhydrazine (APHZ) on the stromal cells of the bone marrow in rats (in vitro) [20].

Associations of Pyrodin with other chemical compounds

• Severely diminished glutathione stability during incubation with acetylphenylhydrazine was observed in the erythrocytes of the siblings, as well as intermediate stability in the parents' red cells [21].
• The complexes 1 + 2 and 2 + 4.9 were also seen in acetylphenylhydrazine-treated, glucose-6-phosphate dehydrogenase-deficient fresh erythrocytes that showed marked GSH depletion but preserved greater than 70% of the original ATP level [22].
• Ascorbate was found to inhibit oxidation of oxyhaemoglobin and Heinz body formation in glucose-6-phosphate dehydrogenase deficient red cells incubated with acetylphenylhydrazine [23].
• 2 Application of this test to the blood of eight normal volunteers and twelve known G-6-PD deficient persons demonstrated that acetylphenylhydrazine and primaquine produced a significant decrease in GSH levels in G-6-PD deficient red cells compared to the reduction seen in normal red cells incubated with these drugs [24].
• The haemolytic reactions of 1-acetyl-2-phenylhydrazine and hydrazine: a spin trapping study [25].

Gene context of Pyrodin

• When exposed to acetylphenylhydrazine, erythrocytes from the G6PD-deficient colt produced more and smaller Heinz bodies than did erythrocytes from normal horses [26].
• Acetylphenylhydrazine (0.016 mol/l), 2. diamide (0.5 mmol/l), 3. osmotic swelling (200 mosm/l) and osmotic shrinking (480 mosm/l) by suspending the cells in hypo- and hypertonic saline [27].
• The abnormally slow rate of Hb F oxidation was even more striking when the oxidant was acetylphenylhydrazine (APH), and the sensitivity of the reaction to catalase was severely diminished [28].
• A procedure is described for preparing a fraction highly enriched for chicken blood delta-aminolevulinate synthase (ALA-S) using animals recovering from acetylphenylhydrazine-induced anemia [29].

Analytical, diagnostic and therapeutic context of Pyrodin

• Moreover, erythrocytes of healthy adults containing Heinz bodies after incubation with acetylphenylhydrazine showed a decreased filtration rate as well as an increased viscosity [30].
• For this reason, we studied the in vitro formation of methemoglobin at 0' and 90', dosed following the HPLC method, after oxidative stress of blood by means of acetylphenylhydrazine in 40 subjects with cancer and 40 healthy donors [31].
• Newts, Triturus cristatus carnifex (Laurenti), were anesthetized by submersion in 2% chlorbutol in tap water for 15 min, splenectomized and then rendered totally anemic two months later by treatment with acetylphenylhydrazine (APH) diluted in their tanks (25 mg/liter for 36 h, changing the solution every 12 h) [32].
• Hemolytic anemia was induced in rats by single intraperitoneal injection of acetylphenylhydrazine (APH) and serum was obtained from the rats on day 1 after APH injection [33].
• Acetylphenylhydrazine induced haemoglobin oxidation in erythrocytes studied by Mössbauer spectroscopy [34].

References