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Gene Review

uvrA  -  excinuclease ABC subunit A

Escherichia coli CFT073

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Disease relevance of uvrA


High impact information on uvrA

  • We confirm that the uvrA gene product is induced by DNA damage in a recA+lexA+-dependent fashion [6].
  • We have investigated this possibility further and describe here the generation and characterization of a set of fusions of the lac genes to the promoter of the uvrA gene [6].
  • MFD is abolished by mutations in the uvrA, -B, or -C genes, which prevent excision repair, or by a mfd mutation, which reduces the rate of excision but does not affect survival [7].
  • The denV gene was found to be expressed, probably from its own promoter, causing pyrimidine dimer incision-deficient uvrA, uvrB, uvrC strains to be rescued by the denV gene [8].
  • The cloned uvrA and uvrB genes of Escherichia coli K-12 were amplified by linkage to the PL promoter of plasmid pKC30 [9].

Chemical compound and disease context of uvrA


Biological context of uvrA

  • We have constructed a hybrid pKC30-uvrA plasmid (pGHY5003) in which transcription of the uvrA gene can be induced under pL control to amplify the uvrA gene product to 7% of cellular protein [1].
  • N'-Methyl-N4-aminocytidine, N'-(2-hydroxyethyl)-N4-aminocytidine and N',N'-dimethyl-N4-aminocytidine showed direct-acting mutagenicity on S. typhimurium TA100 and E. coli WP2 uvrA, tester strains that are sensitive to base-pair substitutions [15].
  • Upstream of the uvrA+ gene are two large open reading frames, both of which are directionally divergent from the uvrA+ gene [5].
  • Selection for UV resistance, after 5-bromouracil (BU) treatment of E. coli B/r uvrA lexA-102, has yielded derivatives more resistant than lexA but still refractory to UV mutagenesis [16].
  • The epoxides were assayed for mutagenicity in the absence of metabolic activation in S. typhimurium TA1535 and E. coli WP2 uvrA and for preferential inhibition of growth of DNA-repair-deficient E. coli [17].

Anatomical context of uvrA


Associations of uvrA with chemical compounds

  • However, chloramphenicol did not affect strand joining: (a) in uvrA bacteria allowed 20 min of growth between irradiation and antibiotic treatment; (b) in nonmutable uvrA exrA bacteria; and (c) in uvrA tif bacteria grown at 42 degrees for 70 min before irradiation [10].
  • Three distinct sections of the ultraviolet mutation frequency response (MFR) curve toward tryptophan prototrophy have been demonstrated in Excherichia coli B/r WP2 trp thy and its uvrA derivative in log-phase growth in minimal medium [21].
  • Hydroxyurea experiments and thymine starvation of lexA3 and uvrA DNA repair mutants rule out unbalanced growth, the SOS response, and nucleotide excision repair as explanations for TLD [22].
  • It is proposed that pyrimidine dimers, although induced, are not significant 365-nm lethal lesions in uvrA and wild-type strains because of their efficient dark repair [23].
  • It has been found that tannic acid stimulates the excision repair encoded by the uvrA gene thereby reducing the yield of mutants [24].

Other interactions of uvrA

  • In an excision-deficient (uvrA 155) background the yield of UV-induced streptomycin-resistant mutations was lower in gyrB bacteria at all doses; the yield of tryptophan-independent mutations was slightly lower at low doses and slightly higher at high doses [25].

Analytical, diagnostic and therapeutic context of uvrA


  1. Amplification of the uvrA gene product of Escherichia coli to 7% of cellular protein by linkage to the pL promoter of pKC30. Yoakum, G.H., Yeung, A.T., Mattes, W.B., Grossman, L. Proc. Natl. Acad. Sci. U.S.A. (1982) [Pubmed]
  2. Mutagenicity experiments on agroclavines, new natural antineoplastic compounds. Glatt, H., Eich, E., Pertz, H., Becker, C., Oesch, F. Cancer Res. (1987) [Pubmed]
  3. Amplification of single-strand DNA binding protein in Escherichia coli. Chase, J.W., Whittier, R.F., Auerbach, J., Sancar, A., Rupp, W.D. Nucleic Acids Res. (1980) [Pubmed]
  4. Multiple pathways of recombination define cellular responses to cisplatin. Zdraveski, Z.Z., Mello, J.A., Marinus, M.G., Essigmann, J.M. Chem. Biol. (2000) [Pubmed]
  5. Identification and characterization of uvrA, a DNA repair gene of Deinococcus radiodurans. Agostini, H.J., Carroll, J.D., Minton, K.W. J. Bacteriol. (1996) [Pubmed]
  6. Expression of the E. coli uvrA gene is inducible. Kenyon, C.J., Walker, G.C. Nature (1981) [Pubmed]
  7. Escherichia coli mfd mutant deficient in "mutation frequency decline" lacks strand-specific repair: in vitro complementation with purified coupling factor. Selby, C.P., Witkin, E.M., Sancar, A. Proc. Natl. Acad. Sci. U.S.A. (1991) [Pubmed]
  8. Expression of a cloned denV gene of bacteriophage T4 in Escherichia coli. Valerie, K., Henderson, E.E., de Riel, J.K. Proc. Natl. Acad. Sci. U.S.A. (1985) [Pubmed]
  9. Enzymatic properties of purified Escherichia coli uvrABC proteins. Yeung, A.T., Mattes, W.B., Oh, E.Y., Grossman, L. Proc. Natl. Acad. Sci. U.S.A. (1983) [Pubmed]
  10. Inducible error-prone repair in Escherichia coli. Sedgwick, S.G. Proc. Natl. Acad. Sci. U.S.A. (1975) [Pubmed]
  11. Arene imines, a new class of exceptionally potent mutagens in bacterial and mammalian cells. Glatt, H., Ludewig, G., Platt, K.L., Waechter, F., Yona, I., Ben-Shoshan, S., Jerushalmy, P., Blum, J., Oesch, F. Cancer Res. (1985) [Pubmed]
  12. N4-aminocytidine, a nucleoside analog that has an exceptionally high mutagenic activity. Negishi, K., Harada, C., Ohara, Y., Oohara, K., Nitta, N., Hayatsu, H. Nucleic Acids Res. (1983) [Pubmed]
  13. Mutagenicity of soy sauce treated with a physiologically feasible concentration of nitrite. Tahira, T., Fujita, Y., Ochiai, M., Wakabayashi, K., Nagao, M., Sugimura, T. Mutat. Res. (1986) [Pubmed]
  14. Comparisons on chemically-induced mutagenicity among four bacterial strains, Salmonella typhimurium TA102 and TA2638, and Escherichia coli WP2/pKM101 and WP2 uvrA/pKM101: collaborative study I. Watanabe, K., Sakamoto, K., Sasaki, T. Mutat. Res. (1996) [Pubmed]
  15. Direct-acting mutagenicity of N4-aminocytidine derivatives bearing alkyl groups at the hydrazino nitrogens. Nomura, A., Negishi, K., Hayatsu, H. Nucleic Acids Res. (1985) [Pubmed]
  16. Partial suppression of the LexA phenotype by mutations (rnm) which restore ultraviolet resistance but not ultraviolet mutability to Escherichia coli B/r uvr A lexA. Volkert, M.R., George, D.L., Witkin, E.M. Mutat. Res. (1976) [Pubmed]
  17. Mutagenicity of chloroalkene epoxides in bacterial systems. Kline, S.A., McCoy, E.C., Rosenkranz, H.S., Van Duuren, B.L. Mutat. Res. (1982) [Pubmed]
  18. Acrylamide; induction of DNA damage, chromosomal aberrations and cell transformation without gene mutations. Tsuda, H., Shimizu, C.S., Taketomi, M.K., Hasegawa, M.M., Hamada, A., Kawata, K.M., Inui, N. Mutagenesis (1993) [Pubmed]
  19. Expression of recombinant human cytochrome P450 1A2 in Escherichia coli bacterial mutagenicity tester strain. Chun, Y.J. Arch. Pharm. Res. (1998) [Pubmed]
  20. Repair of damage in double-stranded phi X174 (RF) DNA due to radiation-induced water radicals. Nabben, F.J., Lafleur, M.V., Sikkers, J.C., Loman, A.C., Retèl, J., Loman, H. Int. J. Radiat. Biol. Relat. Stud. Phys. Chem. Med. (1984) [Pubmed]
  21. Complexity of the ultraviolet mutation frequency response curve in Escherichia coli B/r: SOS induction, one-lesion and two-lesion mutagenesis. Doudney, C.O. J. Bacteriol. (1976) [Pubmed]
  22. Role of DNA replication and repair in thymineless death in Escherichia coli. Morganroth, P.A., Hanawalt, P.C. J. Bacteriol. (2006) [Pubmed]
  23. Lethal effects of pyrimidine dimers induced at 365 nm in strains of E. coli differing in repair capability. Webb, R.B., Brown, M.S., Tyrrell, R.M. Mutat. Res. (1976) [Pubmed]
  24. Antimutagenesis by factors affecting DNA repair in bacteria. Kuroda, Y., Inoue, T. Mutat. Res. (1988) [Pubmed]
  25. Mutagenic repair in Escherichia coli. VIII. Effect of gyrB mutations on ultraviolet light mutagenesis. Bridges, B.A., Southworth, M.W., Orr, E. Mutat. Res. (1983) [Pubmed]
  26. Urinary mutagenicity tests in lead-exposed workers. Apostoli, P., Leone, R., Porru, S., Fracasso, M.E., Alessio, L. Mutat. Res. (1989) [Pubmed]
  27. Evaluation of the mutagenicity and the tumor-promoting activity of parasite extracts: Schistosoma japonicum and Clonorchis sinensis. Ishii, A., Matsuoka, H., Aji, T., Hayatsu, H., Wataya, Y., Arimoto, S., Tokuda, H. Mutat. Res. (1989) [Pubmed]
  28. Recombinant Brucella abortus proteins that induce proliferation and gamma-interferon secretion by CD4+ T cells from Brucella-vaccinated mice and delayed-type hypersensitivity in sensitized guinea pigs. Oliveira, S.C., Harms, J.S., Banai, M., Splitter, G.A. Cell. Immunol. (1996) [Pubmed]
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