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Gene Review

UL27  -  type 1 membrane protein; contains a signal...

Human herpesvirus 2

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Disease relevance of UL27


High impact information on UL27

  • To accurately characterize the sequence of events involved in the activation and generation of anti-HSV CTLs, we used T cell receptor (TCR) transgenic mice specific for the immunodominant epitope from HSV glycoprotein B (gB(498-505)) [6].
  • Analysis of CD69 up-regulation revealed activation of gB-specific CD8(+) T cells by 6-8 h after infection [6].
  • An approximately 60% reduction in activity was seen against targets infected with mutant virus in the presence of 2dG; these targets express gD, but neither gB nor gC [7].
  • These studies were undertaken to evaluate the frequency and phenotype of the CD8+ T cell population within the PLN, recognizing the single immunodominant HSV-1 epitope derived from the viral envelope glycoprotein, glycoprotein B (gB), using an intracellular IFN-gamma-staining assay [8].
  • Phenotypic analysis of the IFN-gamma-producing gB-specific CD8+ T cells generated in the PLN during the course of the acute infection expressed the CD44high CD25+ phenotype on days 3-5 postinfection [8].

Chemical compound and disease context of UL27

  • Dextran sulfate can act as an artificial receptor to mediate a type-specific herpes simplex virus infection via glycoprotein B [9].
  • Alanine substitution of conserved residues in the cytoplasmic tail of herpes simplex virus gB can enhance or abolish cell fusion activity and viral entry [10].
  • Phenotypic analyses of the insertion mutant, R3145, showed that the alpha gB gene was transcribed in the presence of cycloheximide but underwent partial conversion to the HSV-2 form [11].
  • Antisera raised against the beta-galactosidase fusion protein of recombinant phage lambda-113/2 coding for an 84 amino acid (aa) polypeptide originating from dbp BHV-2 neutralized infectivity of BHV-2 and HSV-1 in the presence of complement and precipitated [3H] glucosamine-labeled gB BHV-2 and gB-1 [12].
  • The alignment of the amino acid sequence of the FHV-1 gB homologue with those of 14 other herpesviruses revealed that 10 cysteine residues were completely conserved [13].

Biological context of UL27


Anatomical context of UL27


Associations of UL27 with chemical compounds

  • Moreover, HSV-induced liposome destabilization could be inhibited by free anti-gD (the same antibody used in TS immunoliposomes) but not by monoclonal anti-HSV glycoprotein B, indicating that the interaction was antigen-specific [24].
  • The second, at codon 523 of the mature gB protein, encodes a valine in KOS and an alanine in ANG [25].
  • We conclude that a transferable alteration in the 670-bp carboxy-terminal portion of the glycoprotein gB gene of 17 hep syn results in both its syncytial phenotype and the unique pattern of disease that it causes but does not result in heparin resistance [26].
  • Treatment of transformed cells with methotrexate at high concentrations (0.6 to 6 microM) increased gB-1 production 10- to 100-fold, because of an amplification of the episomal recombinant [27].
  • Additional features shared with gB include 6 potential N-linked glycosylation sites and 10 highly conserved cysteine residues in the gI extracellular domain [28].

Other interactions of UL27


Analytical, diagnostic and therapeutic context of UL27

  • Immunization with DNA vaccines encoding glycoprotein D or glycoprotein B, alone or in combination, induces protective immunity in animal models of herpes simplex virus-2 disease [32].
  • Upon pc-gB vaccination, the animals developed both gB- and HSV-specific IgG Ab response and the isotype examination revealed a predominance of IgG2a [33].
  • The glycoprotein B- and ICP8-specific polypeptides were identified by immunoprecipitation with specific antisera [17].
  • The gB-2 segment from aa 18 to 75 may constitute a useful reagent for the virus type-specific serodiagnosis of HSV-2 infections [34].
  • Further studies will be required to determine the relative sensitivities and specificities of the assay for gB-2 aa 18 to 75, HSV gG assays, and HSV lysate Western blot assays for detecting virus type-specific antibody responses in acute and chronic HSV-2 infections [34].


  1. Identification of homologues to the human cytomegalovirus US22 gene family in human herpesvirus 6. Efstathiou, S., Lawrence, G.L., Brown, C.M., Barrell, B.G. J. Gen. Virol. (1992) [Pubmed]
  2. Age-related CD8 T cell clonal expansions constrict CD8 T cell repertoire and have the potential to impair immune defense. Messaoudi, I., Lemaoult, J., Guevara-Patino, J.A., Metzner, B.M., Nikolich-Zugich, J. J. Exp. Med. (2004) [Pubmed]
  3. Expression of herpes simplex virus beta and gamma genes integrated in mammalian cells and their induction by an alpha gene product. Sandri-Goldin, R.M., Goldin, A.L., Holland, L.E., Glorioso, J.C., Levine, M. Mol. Cell. Biol. (1983) [Pubmed]
  4. The application of a plasmid DNA encoding IFN-alpha 1 postinfection enhances cumulative survival of herpes simplex virus type 2 vaginally infected mice. Härle, P., Noisakran, S., Carr, D.J. J. Immunol. (2001) [Pubmed]
  5. Identification of an immunodominant cytotoxic T-lymphocyte recognition site in glycoprotein B of herpes simplex virus by using recombinant adenovirus vectors and synthetic peptides. Hanke, T., Graham, F.L., Rosenthal, K.L., Johnson, D.C. J. Virol. (1991) [Pubmed]
  6. Rapid cytotoxic T lymphocyte activation occurs in the draining lymph nodes after cutaneous herpes simplex virus infection as a result of early antigen presentation and not the presence of virus. Mueller, S.N., Jones, C.M., Smith, C.M., Heath, W.R., Carbone, F.R. J. Exp. Med. (2002) [Pubmed]
  7. Relationship between expression of herpes simplex virus glycoproteins and susceptibility of target cells to human natural killer activity. Bishop, G.A., Glorioso, J.C., Schwartz, S.A. J. Exp. Med. (1983) [Pubmed]
  8. Expression of intracellular IFN-gamma in HSV-1-specific CD8+ T cells identifies distinct responding subpopulations during the primary response to infection. Andersen, H., Dempsey, D., Chervenak, R., Jennings, S.R. J. Immunol. (2000) [Pubmed]
  9. Dextran sulfate can act as an artificial receptor to mediate a type-specific herpes simplex virus infection via glycoprotein B. Dyer, A.P., Banfield, B.W., Martindale, D., Spannier, D.M., Tufaro, F. J. Virol. (1997) [Pubmed]
  10. Alanine substitution of conserved residues in the cytoplasmic tail of herpes simplex virus gB can enhance or abolish cell fusion activity and viral entry. Ruel, N., Zago, A., Spear, P.G. Virology (2006) [Pubmed]
  11. A subset of type-specific epitopes map in the amino terminus of herpes simplex virus type 1 glycoprotein B. Kousoulas, K., Arsenakis, M., Pereira, L. J. Gen. Virol. (1989) [Pubmed]
  12. Common epitopes of glycoprotein B map within the major DNA-binding proteins of bovine herpesvirus type 2 (BHV-2) and herpes simplex virus type 1 (HSV-1). Hammerschmidt, W., Conraths, F., Mankertz, J., Buhk, H.J., Pauli, G., Ludwig, H. Virology (1988) [Pubmed]
  13. Identification and nucleotide sequence of a gene in feline herpesvirus type 1 homologous to the herpes simplex virus gene encoding the glycoprotein B. Maeda, K., Horimoto, T., Norimine, J., Kawaguchi, Y., Tomonaga, K., Niikura, M., Kai, C., Takahashi, E., Mikami, T. Arch. Virol. (1992) [Pubmed]
  14. UL27.5 is a novel gamma2 gene antisense to the herpes simplex virus 1 gene encoding glycoprotein B. Chang, Y.E., Menotti, L., Filatov, F., Campadelli-Fiume, G., Roizman, B. J. Virol. (1998) [Pubmed]
  15. Herpes simplex virus (HSV) glycoproteins B and K inhibit cell fusion induced by HSV syncytial mutants. Hutchinson, L., Graham, F.L., Cai, W., Debroy, C., Person, S., Johnson, D.C. Virology (1993) [Pubmed]
  16. The alpha protein ICP0 does not appear to play a major role in the regulation of herpes simplex virus gene expression during infection in tissue culture. Sandri-Goldin, R.M., Sekulovich, R.E., Leary, K. Nucleic Acids Res. (1987) [Pubmed]
  17. Characterization of the major mRNAs transcribed from the genes for glycoprotein B and DNA-binding protein ICP8 of herpes simplex virus type 1. Rafield, L.F., Knipe, D.M. J. Virol. (1984) [Pubmed]
  18. Role of glycoprotein B of herpes simplex virus type 1 in viral entry and cell fusion. Cai, W.H., Gu, B., Person, S. J. Virol. (1988) [Pubmed]
  19. Immunization with a single major histocompatibility complex class I-restricted cytotoxic T-lymphocyte recognition epitope of herpes simplex virus type 2 confers protective immunity. Blaney, J.E., Nobusawa, E., Brehm, M.A., Bonneau, R.H., Mylin, L.M., Fu, T.M., Kawaoka, Y., Tevethia, S.S. J. Virol. (1998) [Pubmed]
  20. Effects of truncation of the carboxy terminus of pseudorabies virus glycoprotein B on infectivity. Nixdorf, R., Klupp, B.G., Karger, A., Mettenleiter, T.C. J. Virol. (2000) [Pubmed]
  21. Truncation of the carboxy-terminal 28 amino acids of glycoprotein B specified by herpes simplex virus type 1 mutant amb1511-7 causes extensive cell fusion. Baghian, A., Huang, L., Newman, S., Jayachandra, S., Kousoulas, K.G. J. Virol. (1993) [Pubmed]
  22. Truncation of herpes simplex virus type 2 glycoprotein B increases its cell surface expression and activity in cell-cell fusion, but these properties are unrelated. Fan, Z., Grantham, M.L., Smith, M.S., Anderson, E.S., Cardelli, J.A., Muggeridge, M.I. J. Virol. (2002) [Pubmed]
  23. The carboxy-terminal 41 amino acids of herpes simplex virus type 1 glycoprotein B are not essential for production of infectious virus particles. Huff, V., Cai, W., Glorioso, J.C., Levine, M. J. Virol. (1988) [Pubmed]
  24. Interactions of target-sensitive immunoliposomes with herpes simplex virus. The foundation of a sensitive immunoliposome assay for the virus. Ho, R.J., Rouse, B.T., Huang, L. J. Biol. Chem. (1987) [Pubmed]
  25. Glycoprotein B is a specific determinant of herpes simplex virus type 1 neuroinvasiveness. Yuhasz, S.A., Stevens, J.G. J. Virol. (1993) [Pubmed]
  26. Altered pathogenesis in herpes simplex virus type 1 infection due to a syncytial mutation mapping to the carboxy terminus of glycoprotein B. Goodman, J.L., Engel, J.P. J. Virol. (1991) [Pubmed]
  27. Protection from herpes simplex virus type 1 lethal and latent infections by secreted recombinant glycoprotein B constitutively expressed in human cells with a BK virus episomal vector. Manservigi, R., Grossi, M.P., Gualandri, R., Balboni, P.G., Marchini, A., Rotola, A., Rimessi, P., Di Luca, D., Cassai, E., Barbanti-Brodano, G. J. Virol. (1990) [Pubmed]
  28. Comparison of the bovine herpesvirus 1 gI gene and the herpes simplex virus type 1 gB gene. Whitbeck, J.C., Bello, L.J., Lawrence, W.C. J. Virol. (1988) [Pubmed]
  29. Transcriptional and genetic analyses of the herpes simplex virus type 1 genome: coordinates 0.29 to 0.45. Holland, L.E., Sandri-Goldin, R.M., Goldin, A.L., Glorioso, J.C., Levine, M. J. Virol. (1984) [Pubmed]
  30. The herpes simplex virus type 1 alpha protein ICP27 can act as a trans-repressor or a trans-activator in combination with ICP4 and ICP0. Sekulovich, R.E., Leary, K., Sandri-Goldin, R.M. J. Virol. (1988) [Pubmed]
  31. Abundant expression of herpes simplex virus glycoprotein gB using an adenovirus vector. Johnson, D.C., Ghosh-Choudhury, G., Smiley, J.R., Fallis, L., Graham, F.L. Virology (1988) [Pubmed]
  32. Immunization with DNA vaccines encoding glycoprotein D or glycoprotein B, alone or in combination, induces protective immunity in animal models of herpes simplex virus-2 disease. McClements, W.L., Armstrong, M.E., Keys, R.D., Liu, M.A. Proc. Natl. Acad. Sci. U.S.A. (1996) [Pubmed]
  33. Genetic immunization against herpes simplex virus. Protection is mediated by CD4+ T lymphocytes. Manickan, E., Rouse, R.J., Yu, Z., Wire, W.S., Rouse, B.T. J. Immunol. (1995) [Pubmed]
  34. Locations of herpes simplex virus type 2 glycoprotein B epitopes recognized by human serum immunoglobulin G antibodies. Goade, D.E., Bell, R., Yamada, T., Mertz, G.J., Jenison, S. J. Virol. (1996) [Pubmed]
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