The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

Mcpt1  -  mast cell protease 1

Mus musculus

Synonyms: AV080368, Mast cell protease 1, Mcp-1, mMCP-1
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of Mcpt1


High impact information on Mcpt1

  • Neither worm fecundity nor worm burdens were altered in Nippostrongylus-infected mMCP-1(-/)- BALB/c mice [6].
  • CONCLUSIONS: These results imply that mast cells may contribute to the induction of protective Th2 responses and, importantly, that the intestinal inflammation associated with gastrointestinal helminths is partly mediated by mMCP-1 [7].
  • Additionally, levels of TNF-alpha and nitric oxide were significantly reduced in both W/Wv and mMCP-1 deficient mice [7].
  • In vivo, this immunization completely abrogated the increase in mast-cell protease-1 levels as well as the eosinophilia observed in mice after implantation of an IL-9-secreting tumor [8].
  • Supplementation of the same three cytokines with transforming growth factor-beta1 (TGF-beta1; 1 ng/mL) resulted in substantially enhanced expression (6 micrograms/10(6) mBMMC) and extracellular release (2 micrograms/mL of culture supernatant) of mMCP-1 [9].

Chemical compound and disease context of Mcpt1


Biological context of Mcpt1

  • These enzymes were closely related antigenically on Western blotting and by Ouchterlony double diffusion using a polyclonal, cross-absorbed, sheep antibody raised against mouse mast cell protease-1 (MMCP-1) and on the basis of N-terminal amino acid sequence analysis, were identified as variant forms of MMCP-1 [12].
  • The 5' regulatory regions of MMCP-1 and MMCP-L share a remarkably high degree of sequence identity (98%), starting 10 base pairs downstream of exon 1 and extending to the end of the presently sequenced region at position -1347 of the MMCP-1 gene [13].
  • KITG559 and KITV814 also influenced the transcriptional phenotype of mouse mast cell proteases (MMCP) in IC-2 cells [14].
  • A region of approximately 4 kb covering the five exons and 930 bp 5' and 280 bp 3' flanking sequences of the gene for MMCP-1 was characterized by nucleotide sequence analysis [15].
  • The purpose of this study was to determine the kinetics of mMCP-1 transcription and expression in vivo during nematode-induced IMMC hyperplasia [5].

Anatomical context of Mcpt1


Associations of Mcpt1 with chemical compounds


Regulatory relationships of Mcpt1

  • CONCLUSION: The culture conditions that promote mMCP-1 expression and release by MMC homologues also promote the expression and release of CCL2 [22].
  • The normally down-regulatory functions of TGF-beta1 in haematopoiesis are superseded in this culture system by its ability to promote the early expression of alphaE and mMCP-1 [23].

Other interactions of Mcpt1

  • Biochemical and immunological characterization of multiple glycoforms of mouse mast cell protease 1: comparison with an isolated murine serosal mast cell protease (MMCP-4) [12].
  • The amino acid identity was of 74% with RMCP2, and of 76%, 65% and 90% with the mouse proteases MMCP1, MMCP2 and MMCP4, respectively [24].
  • As the key cytokine mediating differentiation of mouse mast cell protease-1-expressing MMC homologues in vitro, TGF-beta1 was considered a likely candidate for regulation of the integrins that facilitate intraepithelial migration of MMC [25].
  • Time course analyses revealed early (within 48 h of infection) induction of Siat4c, Sprr2A, and Relmbeta and later (within 120 h) induction of Mcpt-1 and -2 [26].
  • RESULTS: mMCP-1 colocalized with Golgi matrix protein 130 but was most abundant in the granules, whereas CCL2 was not found in the granules but appeared to be located uniquely in the Golgi complex [22].

Analytical, diagnostic and therapeutic context of Mcpt1


  1. Mast cell protease 5 mediates ischemia-reperfusion injury of mouse skeletal muscle. Abonia, J.P., Friend, D.S., Austen, W.G., Moore, F.D., Carroll, M.C., Chan, R., Afnan, J., Humbles, A., Gerard, C., Knight, P., Kanaoka, Y., Yasuda, S., Morokawa, N., Austen, K.F., Stevens, R.L., Gurish, M.F. J. Immunol. (2005) [Pubmed]
  2. Histochemical and ultrastructural modification of mucosal mast cell granules in parasitized mice lacking the beta-chymase, mouse mast cell protease-1. Wastling, J.M., Knight, P., Ure, J., Wright, S., Thornton, E.M., Scudamore, C.L., Mason, J., Smith, A., Miller, H.R. Am. J. Pathol. (1998) [Pubmed]
  3. Modulation of intestinal muscle contraction by interleukin-9 (IL-9) or IL-9 neutralization: correlation with worm expulsion in murine nematode infections. Khan, W.I., Richard, M., Akiho, H., Blennerhasset, P.A., Humphreys, N.E., Grencis, R.K., Van Snick, J., Collins, S.M. Infect. Immun. (2003) [Pubmed]
  4. Mucosal defense against gastrointestinal nematodes: responses of mucosal mast cells and mouse mast cell protease 1 during primary strongyloides venezuelensis infection in FcRgamma-knockout mice. Onah, D.N., Uchiyama, F., Nagakui, Y., Ono, M., Takai, T., Nawa, Y. Infect. Immun. (2000) [Pubmed]
  5. Constitutive expression of mouse mast cell protease-1 in normal BALB/c mice and its up-regulation during intestinal nematode infection. Wastling, J.M., Scudamore, C.L., Thornton, E.M., Newlands, G.F., Miller, H.R. Immunology (1997) [Pubmed]
  6. Delayed expulsion of the nematode Trichinella spiralis in mice lacking the mucosal mast cell-specific granule chymase, mouse mast cell protease-1. Knight, P.A., Wright, S.H., Lawrence, C.E., Paterson, Y.Y., Miller, H.R. J. Exp. Med. (2000) [Pubmed]
  7. Mouse mast cell protease-1 is required for the enteropathy induced by gastrointestinal helminth infection in the mouse. Lawrence, C.E., Paterson, Y.Y., Wright, S.H., Knight, P.A., Miller, H.R. Gastroenterology (2004) [Pubmed]
  8. Anti-IL-9 vaccination prevents worm expulsion and blood eosinophilia in Trichuris muris-infected mice. Richard, M., Grencis, R.K., Humphreys, N.E., Renauld, J.C., Van Snick, J. Proc. Natl. Acad. Sci. U.S.A. (2000) [Pubmed]
  9. A novel function for transforming growth factor-beta1: upregulation of the expression and the IgE-independent extracellular release of a mucosal mast cell granule-specific beta-chymase, mouse mast cell protease-1. Miller, H.R., Wright, S.H., Knight, P.A., Thornton, E.M. Blood (1999) [Pubmed]
  10. Temporal distribution of distinct mast cell phenotypes during intestinal schistosomiasis in mice. De Jonge, F., Van Nassauw, L., Van Meir, F., Miller, H.R., Van Marck, E., Timmermans, J.P. Parasite Immunol. (2002) [Pubmed]
  11. Enteric expression of the integrin alpha(v)beta(6) is essential for nematode-induced mucosal mast cell hyperplasia and expression of the granule chymase, mouse mast cell protease-1. Knight, P.A., Wright, S.H., Brown, J.K., Huang, X., Sheppard, D., Miller, H.R. Am. J. Pathol. (2002) [Pubmed]
  12. Biochemical and immunological characterization of multiple glycoforms of mouse mast cell protease 1: comparison with an isolated murine serosal mast cell protease (MMCP-4). Newlands, G.F., Knox, D.P., Pirie-Shepherd, S.R., Miller, H.R. Biochem. J. (1993) [Pubmed]
  13. Genes for mast-cell serine protease and their molecular evolution. Huang, R., Hellman, L. Immunogenetics (1994) [Pubmed]
  14. Transforming and differentiation-inducing potential of constitutively activated c-kit mutant genes in the IC-2 murine interleukin-3-dependent mast cell line. Hashimoto, K., Tsujimura, T., Moriyama, Y., Yamatodani, A., Kimura, M., Tohya, K., Morimoto, M., Kitayama, H., Kanakura, Y., Kitamura, Y. Am. J. Pathol. (1996) [Pubmed]
  15. Cloning and structural analysis of MMCP-1, MMCP-4 and MMCP-5, three mouse mast cell-specific serine proteases. Huang, R.Y., Blom, T., Hellman, L. Eur. J. Immunol. (1991) [Pubmed]
  16. Alteration of protease expression phenotype of mouse peritoneal mast cells by changing the microenvironment as demonstrated by in situ hybridization histochemistry. Lee, Y.M., Jippo, T., Kim, D.K., Katsu, Y., Tsujino, K., Morii, E., Kim, H.M., Adachi, S., Nawa, Y., Kitamura, Y. Am. J. Pathol. (1998) [Pubmed]
  17. Immunological relationships during primary infection with Heligmosomoides polygyrus: Th2 cytokines and primary response phenotype. Wahid, F.N., Behnke, J.M., Grencis, R.K., Else, K.J., Ben-Smith, A.W. Parasitology (1994) [Pubmed]
  18. IL-10 induces transcription of the gene for mouse mast cell protease-1, a serine protease preferentially expressed in mucosal mast cells of Trichinella spiralis-infected mice. Ghildyal, N., McNeil, H.P., Stechschulte, S., Austen, K.F., Silberstein, D., Gurish, M.F., Somerville, L.L., Stevens, R.L. J. Immunol. (1992) [Pubmed]
  19. Mast cell-dependent control of Giardia lamblia infections in mice. Li, E., Zhou, P., Petrin, Z., Singer, S.M. Infect. Immun. (2004) [Pubmed]
  20. Mouse mast cell protease-1 cleaves angiotensin I to form angiotensin II. Saito, K., Muto, T., Tomimori, Y., Imajo, S., Maruoka, H., Tanaka, T., Yamashiro, K., Fukuda, Y. Biochem. Biophys. Res. Commun. (2003) [Pubmed]
  21. Murine model of food allergy after epicutaneous sensitization: Role of mucosal mast cell protease-1. Vaali, K., Puumalainen, T.J., Lehto, M., Wolff, H., Rita, H., Alenius, H., Palosuo, T. Scand. J. Gastroenterol. (2006) [Pubmed]
  22. Constitutive secretion of the granule chymase mouse mast cell protease-1 and the chemokine, CCL2, by mucosal mast cell homologues. Brown, J.K., Knight, P.A., Wright, S.H., Thornton, E.M., Miller, H.R. Clin. Exp. Allergy (2003) [Pubmed]
  23. Transforming growth factor-beta1 mediates coexpression of the integrin subunit alphaE and the chymase mouse mast cell protease-1 during the early differentiation of bone marrow-derived mucosal mast cell homologues. Wright, S.H., Brown, J., Knight, P.A., Thornton, E.M., Kilshaw, P.J., Miller, H.R. Clin. Exp. Allergy (2002) [Pubmed]
  24. Molecular cloning of rat mast cell protease 1 and development of specific probes for its gene transcript. Rouleau, A., Garbarg, M., Schwartz, J.C., Ruat, M. Biochem. Biophys. Res. Commun. (1994) [Pubmed]
  25. TGF-beta 1 regulates adhesion of mucosal mast cell homologues to laminin-1 through expression of integrin alpha 7. Rosbottom, A., Scudamore, C.L., von der Mark, H., Thornton, E.M., Wright, S.H., Miller, H.R. J. Immunol. (2002) [Pubmed]
  26. Expression profiling reveals novel innate and inflammatory responses in the jejunal epithelial compartment during infection with Trichinella spiralis. Knight, P.A., Pemberton, A.D., Robertson, K.A., Roy, D.J., Wright, S.H., Miller, H.R. Infect. Immun. (2004) [Pubmed]
WikiGenes - Universities