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Gene Review

Neu2  -  neuraminidase 2

Mus musculus

Synonyms: Cytosolic sialidase, MBS, MSS, MTS, Mouse skeletal muscle sialidase, ...
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Disease relevance of Neu2

  • A cytosolic sialidase cDNA was transfected into a highly metastatic and invasive cell line, B16-BL6, derived from the murine B16 melanoma [1].
  • In vitro effects of baicalein and baicalin treatment on human prostate cancer cell lines DU-145 and PC-3 were assessed by employing cell proliferation (MTS) assay, cytotoxicity (LIVE/DEAD) assay, and TUNEL assay [2].
  • The effects of the oils were examined in PANC-1 human pancreas cancer cells and Crl mouse breast cancer cells in concentrations ranging from 10-400 microg/ml, measuring the reduction of the tetrazolium salt 3-(4,5-dimethylthiazol-2-yl) -5- (3-carboxymethoxyphenyl)-2- (4-sulphophenyl) -2H-tetrazolium (MTS) by mitochondrial enzymes [3].
  • In this paper, we demonstrate that the antibody response toward V3-based synthetic MAPs derived from HIV-1, JY1 isolate, conjugated to two different carrier proteins using either m-maleimidobenzoyl-N-hydroxysuccinimide ester (MBS) or beta-maleimidopropionic acid N-hydroxysuccinimide ester (MPS), or succinic anhydride (SA) show different behaviors [4].
  • Additionally by employing the 'dilution to extinction' method we showed that MSS supported B. pertussis growth from smaller inocula than the original SS medium [5].

High impact information on Neu2

  • Mouse stromal cell lines (MSS lines) have been established from the spleens of newborn mice in culture at a low serum concentration [6].
  • These MSS lines support the proliferation and differentiation of the erythroid progenitor cells from mouse fetal livers and bone marrow in a semisolid medium in the presence of erythropoietin [6].
  • Larger colonies of over 1,000 benzidine-positive erythroid cells were developed from the fetal liver cells on the MSS cell layers after 6 days of incubation [6].
  • Infectivity, syncytium formation, and cytotoxicity of recombinant MV-Edm in HCC cell lines were evaluated by fluorescence microscopy, crystal violet staining, and the MTS assay [7].
  • On the basis of these molecular properties and expression pattern, it is therefore postulated that MTS 23 may detect a novel accessory molecule important for T cell activation [8].

Chemical compound and disease context of Neu2


Biological context of Neu2


Anatomical context of Neu2

  • In this investigation, we cloned a novel cDNA for mouse brain sialidase and expressed the cDNA in COS-7 cells [11].
  • MTS has high and relatively low homologies with those of mammalian cytosolic sialidases from the mouse brain (99%), rat (91%), and human skeletal muscle (75%), and those of the mouse lysosomal (47%) and membrane-bound (51%) sialidases, respectively [10].
  • T-cells also have significant levels of Neu-3 mRNAs, albeit much lower than those of Neu-1, whereas the levels of Neu-2 mRNAs are minimal [14].
  • Moreover, New Zealand mice, unlike controls, were found to have MTS 10+ epithelial cells within the cortex [15].
  • In this study, the functional relevance of this molecule to thymopoiesis was investigated by the addition of purified MTS 35 to fetal thymus organ culture [16].

Associations of Neu2 with chemical compounds

  • Human anti-N-acetyl-D-glucosamine (GlcNAc) antibodies were prepared by affinity chromatography from serum of a healthy donor (MSS) [17].
  • Cell proliferation was assessed with an MTS assay and bromodeoxyuridine (BrdU) uptake [18].
  • HX (10(-4) M) was found to potentiate only the cytotoxicity of DHFR inhibitors (MTS and PTX), increasing cell kill by 20-70 fold to the level achieved by an equivalent concentration (10(-5) M) of ICI 198583 alone [19].
  • Cytotoxicity evaluations carried out by the formazan (MTS) assay showed that the thiolated gelatin prepared with 20 mg and 40 mg of 2-iminothiolane (SHGel-20 and SHGel-40) per gram of gelatin had comparable cell viability profile to that of the unmodified gelatin [20].
  • Furthermore, with both HT-2 and CTLL-2 cells it was found that cultures could be simultaneously labeled with MTS/PMS and [3H]thymidine, with relatively little effect of the dye on uptake of the latter [21].

Other interactions of Neu2

  • To show the existence of this pathway, we have used recombinant mammalian cytosolic sialidase and membrane-associated sialidase to study the desialylation of G(M1) and G(M2) [22].

Analytical, diagnostic and therapeutic context of Neu2

  • Molecular cloning and expression of mouse brain sialidase [11].
  • Soluble Fas ligand-mediated cytotoxicity and apoptosis were evaluated by means of MTS and TUNEL assays, respectively [23].
  • MTS-antibodies enter the living cells in culture and can be detected by immunofluorescence and ELISA after extraction [24].
  • Cells from BM isolated on FMB (FMB-BM cells) were visualized by fluorescent confocal microscopy and quantified by a modified MTS colorimetric assay [25].
  • These compounds were examined for their in vitro antiviral activity by two different bioassays, namely; crystal violet staining and tetrazolium dye (MTS) measurement [26].


  1. Suppression of pulmonary metastasis in murine B16 melanoma cells by transfection of a sialidase cDNA. Tokuyama, S., Moriya, S., Taniguchi, S., Yasui, A., Miyazaki, J., Orikasa, S., Miyagi, T. Int. J. Cancer (1997) [Pubmed]
  2. In vivo and in vitro effect of baicalein on human prostate cancer cells. Miocinovic, R., McCabe, N.P., Keck, R.W., Jankun, J., Hampton, J.A., Selman, S.H. Int. J. Oncol. (2005) [Pubmed]
  3. The cytotoxic effect of two chemotypes of essential oils from the fruits of Angelica archangelica L. Sigurdsson, S., Ogmundsdottir, H.M., Gudbjarnason, S. Anticancer Res. (2005) [Pubmed]
  4. Study of different coupling agents in the conjugation of a V3-based synthetic MAP to carrier proteins. Cruz, L.J., Iglesias, E., Aguilar, J.C., Quintana, D., Garay, H.E., Duarte, C., Reyes, O. J. Pept. Sci. (2001) [Pubmed]
  5. Stainer and Scholte's pertussis medium with an alternative buffer. Lothe, R.A., Frøholm, L.O., Westre, G., Kjennerud, U. Journal of biological standardization. (1985) [Pubmed]
  6. Spleen stromal cell lines selectively support erythroid colony formation. Yanai, N., Matsuya, Y., Obinata, M. Blood (1989) [Pubmed]
  7. Engineered measles virus as a novel oncolytic viral therapy system for hepatocellular carcinoma. Blechacz, B., Splinter, P.L., Greiner, S., Myers, R., Peng, K.W., Federspiel, M.J., Russell, S.J., Larusso, N.F. Hepatology (2006) [Pubmed]
  8. A molecule expressed on accessory cells, activated T cells, and thymic epithelium is a marker and promoter of T cell activation. Izon, D.J., Jones, L.A., Eynon, E.E., Kruisbeek, A.M. J. Immunol. (1994) [Pubmed]
  9. Use of an aqueous soluble tetrazolium/formazan assay for cell growth assays in culture. Cory, A.H., Owen, T.C., Barltrop, J.A., Cory, J.G. Cancer Commun. (1991) [Pubmed]
  10. Cloning, chromosomal mapping, and characteristic 5'-UTR sequence of murine cytosolic sialidase. Kotani, K., Kuroiwa, A., Saito, T., Matsuda, Y., Koda, T., Kijimoto-Ochiai, S. Biochem. Biophys. Res. Commun. (2001) [Pubmed]
  11. Molecular cloning and expression of mouse brain sialidase. Fronda, C.L., Zeng, G., Gao, L., Yu, R.K. Biochem. Biophys. Res. Commun. (1999) [Pubmed]
  12. Genetically modified CD34+ cells exert a cytotoxic bystander effect on human endothelial and cancer cells. Arafat, W.O., Casado, E., Wang, M., Alvarez, R.D., Siegal, G.P., Glorioso, J.C., Curiel, D.T., Gómez-Navarro, J. Clin. Cancer Res. (2000) [Pubmed]
  13. A novel tetraester construct that reduces cationic lipid-associated cytotoxicity. Implications for the onset of cytotoxicity. Aberle, A.M., Tablin, F., Zhu, J., Walker, N.J., Gruenert, D.C., Nantz, M.H. Biochemistry (1998) [Pubmed]
  14. Induction of lysosomal and plasma membrane-bound sialidases in human T-cells via T-cell receptor. Wang, P., Zhang, J., Bian, H., Wu, P., Kuvelkar, R., Kung, T.T., Crawley, Y., Egan, R.W., Billah, M.M. Biochem. J. (2004) [Pubmed]
  15. Thymic microenvironmental abnormalities and thymic selection in NZB.H-2bm12 mice. Watanabe, Y., Naiki, M., Wilson, T., Godfrey, D., Chiang, B.L., Boyd, R., Ansari, A., Gershwin, M.E. J. Immunol. (1993) [Pubmed]
  16. A lymphostromal molecule, thymic shared Ag-1, regulates early thymocyte development in fetal thymus organ culture. Randle, E.S., Waanders, G.A., Masciantonio, M., Godfrey, D.I., Boyd, R.L. J. Immunol. (1993) [Pubmed]
  17. Idiotope mapping on the variable region of an antibody clonotype produced by normal (nonmalignant) human B cells. Zenke, G., Eichmann, K., Emmrich, F. J. Immunol. (1985) [Pubmed]
  18. Distinct functions for Ras GTPases in the control of proliferation and apoptosis in mouse and human mesangial cells. Hendry, B.M., Khwaja, A., Qu, Q.Y., Shankland, S.J. Kidney Int. (2006) [Pubmed]
  19. DNA fragmentation, dATP pool elevation and potentiation of antifolate cytotoxicity in L1210 cells by hypoxanthine. Kwok, J.B., Tattersall, M.H. Br. J. Cancer (1992) [Pubmed]
  20. Preparation and evaluation of thiol-modified gelatin nanoparticles for intracellular DNA delivery in response to glutathione. Kommareddy, S., Amiji, M. Bioconjug. Chem. (2005) [Pubmed]
  21. Use of an aqueous soluble tetrazolium/formazan assay to measure viability and proliferation of lymphokine-dependent cell lines. Buttke, T.M., McCubrey, J.A., Owen, T.C. J. Immunol. Methods (1993) [Pubmed]
  22. Degradation of G(M1) and G(M2) by mammalian sialidases. Li, S.C., Li, Y.T., Moriya, S., Miyagi, T. Biochem. J. (2001) [Pubmed]
  23. Induction of apoptosis in malignant pleural mesothelioma cells by activation of the Fas (Apo-1/CD95) death-signal pathway. Stewart, J.H., Nguyen, D.M., Chen, G.A., Schrump, D.S. J. Thorac. Cardiovasc. Surg. (2002) [Pubmed]
  24. Chemical engineering of cell penetrating antibodies. Zhao, Y., Lou, D., Burkett, J., Kohler, H. J. Immunol. Methods (2001) [Pubmed]
  25. Fibrin microbeads for isolating and growing bone marrow-derived progenitor cells capable of forming bone tissue. Gurevich, O., Vexler, A., Marx, G., Prigozhina, T., Levdansky, L., Slavin, S., Shimeliovich, I., Gorodetsky, R. Tissue engineering. (2002) [Pubmed]
  26. Evaluation of some pyrazoloquinolines as inhibitors of herpes simplex virus type 1 replication. Bekhit, A.A., El-Sayed, O.A., Aboul-Enein, H.Y., Siddiqui, Y.M., Al-Ahdal, M.N. Arch. Pharm. (Weinheim) (2005) [Pubmed]
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