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Gene Review

NONO  -  non-POU domain containing, octamer-binding

Homo sapiens

Synonyms: 54 kDa nuclear RNA-and DNA-binding protein, 55 kDa nuclear protein, DNA-binding p52/p100 complex, 52 kDa subunit, NMT55, NRB54, ...
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Disease relevance of NONO


High impact information on NONO

  • Here we show that the major interacting-proteins with DJ-1 in dopaminergic neuronal cells are the nuclear proteins p54nrb and pyrimidine tract-binding protein-associated splicing factor (PSF), two multifunctional regulators of transcription and RNA metabolism [6].
  • Thus, DJ-1 is a neuroprotective transcriptional co-activator that may act in concert with p54nrb and PSF to regulate the expression of a neuroprotective genetic program [6].
  • Both DJ-1 and p54nrb block oxidative stress and mutant alpha-synuclein-induced cell death [6].
  • Both subunits contained nuclear export and nuclear retention signals, whereas p54nrb was continuously exported from the nucleus and associated with INS-containing mRNA in the cytoplasm, suggesting its additional role at late steps of mRNA metabolism [2].
  • Thus, the lack of activity of p54nrb in F9 cells is due to an ineffective DNA-binding domain [7].

Biological context of NONO


Anatomical context of NONO

  • Two proteins present in HeLa cell extract, the splicing and transcription-associated factors, PSF and p54nrb/NonO, bound specifically and could be purified to virtual homogeneity by chromatography on immobilized CTD matrices [10].
  • Incubation with NONO induced cell damage in control lymphocytes but did not further damage the already affected untreated SALS lymphocytes [11].
  • By immunoblot inhibition and subcellular (cytosolic, nuclear) cell fractionation we identified a 55 kDa nuclear autoantigen expressed in intestinal, endothelial cells and in fibroblasts which was recognized by IgA antibodies of approximately half of the celiac disease patients and cross-reacted with wheat proteins [12].
  • The development of the acoustics of the auditory periphery of the ferret was examined by measuring the spectral transfer functions (STFs) and the directional characteristics of the outer ears of animals ranging in age from postnatal day 32 (P32) to P54 [13].
  • Changes in the mRNA levels of Jurkat T cells induced by cell-cell contact with adherent cells were also found for M-type pyruvate kinase, for tropomyosin TM30 and for the p54nrb gene product [14].

Associations of NONO with chemical compounds

  • In this study, we investigated the effect of the NO-releasing agent, diethylamine NONOate (NONO), on lymphocytes from patients affected by the sporadic form of ALS (SALS) and controls by flow cytometry [11].
  • In addition, site-directed mutagenesis indicated that the interaction of Pin1 with p54nrb was mediated by three threonine residues located in the proline-rich carboxy-terminal extremity of the protein [15].
  • While the biological action of these molecules are thought to be primarily mediated by ca. 55 kDa nuclear retinoic acid receptors, a number of structurally similar 15-20 kDa proteins are involved in the transport, and possibly metabolism, of these compounds [16].
  • Also, the synthesis of 55-kDa nuclear protein(s) was stimulated throughout the cell cycle in the presence of 3 mM caffeine [17].
  • Coordinate regulation of the proximal and distal steps of the pathway of purine synthesis de nono in WI-L2 human lymphoblasts [18].

Physical interactions of NONO

  • Here we show that PSF interacts with p54nrb, a highly related protein first identified based on cross-reactivity to antibodies against the yeast second-step splicing factor Prpl8 [19].
  • We show that PSP1 interacts in vivo with a subset of the total cellular pool of p54nrb [20].

Regulatory relationships of NONO


Other interactions of NONO


Analytical, diagnostic and therapeutic context of NONO


  1. 55 kDa nuclear matrix protein (nmt55) mRNA is expressed in human prostate cancer tissue and is associated with the androgen receptor. Ishiguro, H., Uemura, H., Fujinami, K., Ikeda, N., Ohta, S., Kubota, Y. Int. J. Cancer (2003) [Pubmed]
  2. PSF acts through the human immunodeficiency virus type 1 mRNA instability elements to regulate virus expression. Zolotukhin, A.S., Michalowski, D., Bear, J., Smulevitch, S.V., Traish, A.M., Peng, R., Patton, J., Shatsky, I.N., Felber, B.K. Mol. Cell. Biol. (2003) [Pubmed]
  3. AFX1 and p54nrb: fine mapping, genomic structure, and exclusion as candidate genes of X-linked dystonia parkinsonism. Peters, U., Haberhausen, G., Kostrzewa, M., Nolte, D., Müller, U. Hum. Genet. (1997) [Pubmed]
  4. Loss of expression of a 55 kDa nuclear protein (nmt55) in estrogen receptor-negative human breast cancer. Traish, A.M., Huang, Y.H., Ashba, J., Pronovost, M., Pavao, M., McAneny, D.B., Moreland, R.B. Diagn. Mol. Pathol. (1997) [Pubmed]
  5. Synthesis of mono- and bifunctional alpha-methylene lactone systems as potential tumor inhibitors. Grieco, P.A., Noguez, J.A., Masaki, Y., Hiroi, Y., Nishizawa, M., Rosowsky, A., Oppenheim, S., Lazarus, H. J. Med. Chem. (1977) [Pubmed]
  6. The Parkinson's disease-associated DJ-1 protein is a transcriptional co-activator that protects against neuronal apoptosis. Xu, J., Zhong, N., Wang, H., Elias, J.E., Kim, C.Y., Woldman, I., Pifl, C., Gygi, S.P., Geula, C., Yankner, B.A. Hum. Mol. Genet. (2005) [Pubmed]
  7. The intracisternal A-particle proximal enhancer-binding protein activates transcription and is identical to the RNA- and DNA-binding protein p54nrb/NonO. Basu, A., Dong, B., Krainer, A.R., Howe, C.C. Mol. Cell. Biol. (1997) [Pubmed]
  8. Fusion of splicing factor genes PSF and NonO (p54nrb) to the TFE3 gene in papillary renal cell carcinoma. Clark, J., Lu, Y.J., Sidhar, S.K., Parker, C., Gill, S., Smedley, D., Hamoudi, R., Linehan, W.M., Shipley, J., Cooper, C.S. Oncogene (1997) [Pubmed]
  9. Mechanism of interferon action. II. Induction and decay kinetics of the antiviral state and protein P54 in human amnion U cells treated with gamma interferon. Ulker, N., Samuel, C.E. J. Biol. Chem. (1987) [Pubmed]
  10. Splicing and transcription-associated proteins PSF and p54nrb/nonO bind to the RNA polymerase II CTD. Emili, A., Shales, M., McCracken, S., Xie, W., Tucker, P.W., Kobayashi, R., Blencowe, B.J., Ingles, C.J. RNA (2002) [Pubmed]
  11. Effect of nitric oxide on lymphocytes from sporadic amyotrophic lateral sclerosis patients: toxic or protective role? Cereda, C., Cova, E., Di Poto, C., Galli, A., Mazzini, G., Corato, M., Ceroni, M. Neurol. Sci. (2006) [Pubmed]
  12. IgA cross-reactivity between a nuclear autoantigen and wheat proteins suggests molecular mimicry as a possible pathomechanism in celiac disease. Natter, S., Granditsch, G., Reichel, G.L., Baghestanian, M., Valent, P., Elfman, L., Grönlund, H., Kraft, D., Valenta, R. Eur. J. Immunol. (2001) [Pubmed]
  13. The auditory periphery of the ferret: postnatal development of acoustic properties. Carlile, S. Hear. Res. (1991) [Pubmed]
  14. Cell-cell contact of human T cells with fibroblasts changes lymphocytic mRNA expression: increased mRNA expression of interleukin-17 and interleukin-17 receptor. Thiele, K., Riemann, D., Navarrete Santos, A., Langner, J., Kehlen, A. Eur. Cytokine Netw. (2000) [Pubmed]
  15. The multifunctional nuclear protein p54nrb is multiphosphorylated in mitosis and interacts with the mitotic regulator Pin1. Proteau, A., Blier, S., Albert, A.L., Lavoie, S.B., Traish, A.M., Vincent, M. J. Mol. Biol. (2005) [Pubmed]
  16. NMR studies of retinoid-protein interactions: the conformation of [13C]-beta-ionones bound to beta-lactoglobulin B. Curley, R.W., Sundaram, A.K., Fowble, J.W., Abildgaard, F., Westler, W.M., Markley, J.L. Pharm. Res. (1999) [Pubmed]
  17. Effects of irradiation on nuclear protein synthesis in G2 phase of the cell cycle. Holland, J.M., Wright, W.D., Higashikubo, R., Roti Roti, J.L. Radiat. Res. (1990) [Pubmed]
  18. Coordinate regulation of the proximal and distal steps of the pathway of purine synthesis de nono in WI-L2 human lymphoblasts. Hershfield, M.S., Seegmiller, J.E. Adv. Exp. Med. Biol. (1977) [Pubmed]
  19. PSF and p54nrb bind a conserved stem in U5 snRNA. Peng, R., Dye, B.T., Pérez, I., Barnard, D.C., Thompson, A.B., Patton, J.G. RNA (2002) [Pubmed]
  20. P54nrb forms a heterodimer with PSP1 that localizes to paraspeckles in an RNA-dependent manner. Fox, A.H., Bond, C.S., Lamond, A.I. Mol. Biol. Cell (2005) [Pubmed]
  21. Purification and cDNA cloning of HeLa cell p54nrb, a nuclear protein with two RNA recognition motifs and extensive homology to human splicing factor PSF and Drosophila NONA/BJ6. Dong, B., Horowitz, D.S., Kobayashi, R., Krainer, A.R. Nucleic Acids Res. (1993) [Pubmed]
  22. Insurance decisions about obesity surgery: a new type of randomization? Choban, P., Lu, B., Flancbaum, L. Obesity surgery : the official journal of the American Society for Bariatric Surgery and of the Obesity Surgery Society of Australia and New Zealand. (2000) [Pubmed]
  23. Prognostic factors after acute subdural hematoma. Dent, D.L., Croce, M.A., Menke, P.G., Young, B.H., Hinson, M.S., Kudsk, K.A., Minard, G., Pritchard, F.E., Robertson, J.T., Fabian, T.C. The Journal of trauma. (1995) [Pubmed]
  24. Novel nuclear and cytoplasmic proteins detected by anti-Zoothamnium arbuscula (Protozoa) spasmin 1 antibody in mammalian cells are dependent on the cell cycle. Itabashi, T., Terasaki, T., Asai, H. J. Biochem. (2004) [Pubmed]
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