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PSMD8  -  proteasome (prosome, macropain) 26S...

Homo sapiens

Synonyms: 26S proteasome non-ATPase regulatory subunit 8, 26S proteasome regulatory subunit RPN12, 26S proteasome regulatory subunit S14, HEL-S-91n, HIP6, ...
 
 
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Disease relevance of PSMD8

  • Lysates of the clones expressing this 15K peptide inhibited the reactivity of the p31 virion protein with AIDS sera, suggesting that it is a fragment of the viral p31 protein [1].
  • Individuals with a positive Western blot result lacking the p31 band should be counseled that, although they may be HIV infected, there is uncertainty about this conclusion [2].
  • RESULTS: Of 421 donors who were positive for HIV-1 by Western blot, 39 (9.3%) met the criteria of possible false positivity because they lacked reactivity to p31 [2].
  • Infection of Neuro2A cells with a retrovirus carrying a human somatostatin cDNA resulted in the expression of prosomatostatin and its processing into S-28 and S-14, indicating that these cells have the necessary enzymes to process prohormone at both single and paired amino acid residues [3].
  • These data directly demonstrate that SREBP-1c drives S14 gene expression in breast cancer cells, and progestin magnifies that effect via an indirect mechanism [4].
 

High impact information on PSMD8

  • Here we report that phosphorylation of histone H2B at serine 14 (S14) correlates with cells undergoing programmed cell death in vertebrates [5].
  • As judged by its resistance to high concentrations of alpha-amanitin, cell-free transcription of alpha-250 and alpha-280 appears to involve RNA polymerase I. Tissue culture transfection and cell-free transcription experiments demonstrate that alpha-250 and alpha-280 stimulate S14 mRNA transcription, whereas free ribosomal protein S14 inhibits it [6].
  • We demonstrate here that in transiently transfected COS cells expressing high levels of the p31 or p41 forms of Ii, uncleaved Ii is transported to and accumulates in transferrin-accessible (early) endosomes [7].
  • Amino acid substitutions were introduced that affected the possibility of forming beta-turn structures in the immediate vicinity of the somatostatin-28 (S-28) and somatostatin-14 (S-14) cleavage sites [3].
  • Sequential analyses of mRNA activity profiles have identified an mRNA sequence (mRNAs14) coding for a protein (S14) with Mr 17 010 and pI 4.9 which responds to triiodothyronine with a lag time of less than 20 minutes [8].
 

Chemical compound and disease context of PSMD8

  • Polystyrene beads of four diameters, each size coated with a different HIV recombinant DNA-produced protein (p24, p31, gp41, or gp120), bound anti-HIV antibodies detected with fluorescent antiglobulin [9].
  • The degree of differences between the S14/C4 and ARV was only 23-24%, and 21-22%, respectively, at both the nucleotide and deduced amino acid levels, suggested that DRVs are quite different from ARVs and should give a precise classification for DRVs in Orthoreovirus genus [10].
  • In the S13, S14, S15, and S16 generations the Flinders S-line of rats were still more sensitive to the effects of DFP on the criterion variables upon which selection was based: core body temperature, body weight and a simple operant response for water reward [11].
 

Biological context of PSMD8

  • Southern blot analysis of DNAs from human-rodent somatic cell hybrids with an isolated human cDNA assigned the gene coding for this enzyme to the short arm of chromosome 1, band p31 [12].
  • While the transient expression of the pro-Sf-caspase-1 did not induce apoptosis, expression of the pro-domain deleted form, p31, or coexpression of the two subunits of mature Sf-caspase-1, p19 and p12, induced apoptosis in Sf-21 cells [13].
  • Lack of introns in the ribosomal protein gene S14 of trypanosomes [14].
  • Two overlapping human genomic S14 DNA clones were isolated from a Charon 28 placental DNA library [15].
  • The human S14 gene consists of five exons and four introns spanning 5.9 kilobase pairs of DNA [15].
 

Anatomical context of PSMD8

  • For the ERF and PSMD8 genes assigned to SSCs other than SSC6, additional mapping using somatic cell hybrid (SCH) panels was performed to confirm the results of RH-mapping [16].
  • In these respects the exogenous human S14 message appeared to function normally in CHO cells [17].
  • Polyadenylated S14 transcripts purified from HeLa cell cytoplasma display heterogeneous 5' ends that map within noncoding RPS14 exon 1 [15].
  • The steady state levels of mRNAs codying for the ribosomal proteins S6, S11, and S14 have been evaluated in quiescent and proliferating human fibroblasts and in resting and proliferating human peripheral blood mononuclear cells [18].
  • The abundance of c-myc, calcyclin, and S14 mRNA in terminally differentiated HL-60 cells decreased sharply, compared to their proliferating counterparts [19].
 

Associations of PSMD8 with chemical compounds

  • Glucose response elements have been characterized for the L-pyruvate kinase and S14 genes [20].
  • In contrast, addition of glucose to HIT cells leads to enhanced tricarboxylic acid cycle activity to oxidize pyruvate and an associated stimulation of S14 transcription [21].
  • Studies to map the regulatory sequences of lipogenic enzyme genes involved in the transcriptional response have been performed for the L-type pyruvate kinase, S14, and acetyl-coenzyme A carboxylase genes [22].
  • Ii is a type II membrane glycoprotein that is synthesized as different isoforms that include a major 31 kDa isoform (p31/p33) and a minor 41 kDa isoform (p41) in both, mice and humans [23].
  • Dominant-negative SREBP-1c inhibited induction of S14 and FAS mRNAs by progestin, while active SREBP-1c induced without hormone and superinduced in its presence [4].
 

Other interactions of PSMD8

 

Analytical, diagnostic and therapeutic context of PSMD8

  • The C-terminal extension of UCH37 is essential for interaction with S14 or UIP1 as shown by the yeast two-hybrid assay and the in vitro binding assay [24].
  • Nonetheless, individual intron splice donor, splice acceptor, and upstream flanking motifs have been conserved within mammalian S14 homologues as well as within RPS14 gene fragments PCR amplified from other vertebrate genera (birds and bony fish) [25].
  • The majority (95%) of serum samples obtained from individuals seropositive in the virus ELISA were also positive in the p31 antibody ELISA [26].
  • Gel permeation chromatography revealed that rCGRP-I increased the secretion of S-14 by 22 +/- 6-fold (P < 0.01) compared to the control value, whereas that of S-28 increased nonsignificantly by only 2 +/- 1-fold [27].
  • This was supported by immunocytochemistry, wherein F-4 reactivity was localized in gastrointestinal (GI) endocrine cells and a widespread plexus of neurons within the wall of the distal gut while immunoreactivity to C-terminal domains of S-14 and S-28 in these neurons was absent [28].

References

  1. An HTLV-III peptide produced by recombinant DNA is immunoreactive with sera from patients with AIDS. Chang, N.T., Huang, J., Ghrayeb, J., McKinney, S., Chanda, P.K., Chang, T.W., Putney, S., Sarngadharan, M.G., Wong-Staal, F., Gallo, R.C. Nature (1985) [Pubmed]
  2. False-positive HIV-1 test results in a low-risk screening setting of voluntary blood donation. Retrovirus Epidemiology Donor Study. Kleinman, S., Busch, M.P., Hall, L., Thomson, R., Glynn, S., Gallahan, D., Ownby, H.E., Williams, A.E. JAMA (1998) [Pubmed]
  3. Site-specific mutagenesis identifies amino acid residues critical in prohormone processing. Gomez, S., Boileau, G., Zollinger, L., Nault, C., Rholam, M., Cohen, P. EMBO J. (1989) [Pubmed]
  4. S14 protein in breast cancer cells: direct evidence of regulation by SREBP-1c, superinduction with progestin, and effects on cell growth. Martel, P.M., Bingham, C.M., McGraw, C.J., Baker, C.L., Morganelli, P.M., Meng, M.L., Armstrong, J.M., Moncur, J.T., Kinlaw, W.B. Exp. Cell Res. (2006) [Pubmed]
  5. Apoptotic phosphorylation of histone H2B is mediated by mammalian sterile twenty kinase. Cheung, W.L., Ajiro, K., Samejima, K., Kloc, M., Cheung, P., Mizzen, C.A., Beeser, A., Etkin, L.D., Chernoff, J., Earnshaw, W.C., Allis, C.D. Cell (2003) [Pubmed]
  6. Regulation of human RPS14 transcription by intronic antisense RNAs and ribosomal protein S14. Tasheva, E.S., Roufa, D.J. Genes Dev. (1995) [Pubmed]
  7. Relationship between invariant chain expression and major histocompatibility complex class II transport into early and late endocytic compartments. Romagnoli, P., Layet, C., Yewdell, J., Bakke, O., Germain, R.N. J. Exp. Med. (1993) [Pubmed]
  8. Thyroid hormone action at the nuclear level. Oppenheimer, J.H. Ann. Intern. Med. (1985) [Pubmed]
  9. Early detection of antibodies against rDNA-produced HIV proteins with a flow cytometric assay. Scillian, J.J., McHugh, T.M., Busch, M.P., Tam, M., Fulwyler, M.J., Chien, D.Y., Vyas, G.N. Blood (1989) [Pubmed]
  10. Characterization of the sigmaC-encoding Gene from Musocvy Duck Reovirus. Zhang, Y., Liu, M., Hu, Q., Ouyang, S., Tong, G. Virus Genes (2006) [Pubmed]
  11. Selective breeding for sensitivity to DFP: generalization of effects beyond criterion variables. Russell, R.W., Overstreet, D.H., Messenger, M., Helps, S.C. Pharmacol. Biochem. Behav. (1982) [Pubmed]
  12. Molecular cloning of cDNAs encoding rat and human medium-chain acyl-CoA dehydrogenase and assignment of the gene to human chromosome 1. Matsubara, Y., Kraus, J.P., Yang-Feng, T.L., Francke, U., Rosenberg, L.E., Tanaka, K. Proc. Natl. Acad. Sci. U.S.A. (1986) [Pubmed]
  13. Baculovirus inhibitors of apoptosis (IAPs) block activation of Sf-caspase-1. Seshagiri, S., Miller, L.K. Proc. Natl. Acad. Sci. U.S.A. (1997) [Pubmed]
  14. Lack of introns in the ribosomal protein gene S14 of trypanosomes. Perelman, D., Boothroyd, J.C. Mol. Cell. Biol. (1990) [Pubmed]
  15. Primary structure of human ribosomal protein S14 and the gene that encodes it. Rhoads, D.D., Dixit, A., Roufa, D.J. Mol. Cell. Biol. (1986) [Pubmed]
  16. Construction of a high-resolution comparative gene map between swine chromosome region 6q11-->q21 and human chromosome 19 q-arm by RH mapping of 51 genes. Bosak, N., Faraut, T., Mikawa, S., Uenishi, H., Kiuchi, S., Hiraiwa, H., Hayashi, T., Yasue, H. Cytogenet. Genome Res. (2003) [Pubmed]
  17. A cloned human ribosomal protein gene functions in rodent cells. Rhoads, D.D., Roufa, D.J. Mol. Cell. Biol. (1987) [Pubmed]
  18. Noncoordinated expression of S6, S11, and S14 ribosomal protein genes in leukemic blast cells. Ferrari, S., Manfredini, R., Tagliafico, E., Rossi, E., Donelli, A., Torelli, G., Torelli, U. Cancer Res. (1990) [Pubmed]
  19. Abundance of the primary transcript and its processed product of growth-related genes in normal and leukemic cells during proliferation and differentiation. Ferrari, S., Tagliafico, E., Manfredini, R., Grande, A., Rossi, E., Zucchini, P., Torelli, G., Torelli, U. Cancer Res. (1992) [Pubmed]
  20. Mechanisms by which carbohydrates regulate expression of genes for glycolytic and lipogenic enzymes. Girard, J., Ferré, P., Foufelle, F. Annu. Rev. Nutr. (1997) [Pubmed]
  21. Cell-specific carbohydrate metabolism regulates S14 gene transcription. Goto, Y., Mariash, C.N. Diabetes (1992) [Pubmed]
  22. Regulation of the expression of lipogenic enzyme genes by carbohydrate. Towle, H.C., Kaytor, E.N., Shih, H.M. Annu. Rev. Nutr. (1997) [Pubmed]
  23. The MHC class II-associated invariant chain: a molecule with multiple roles in MHC class II biosynthesis and antigen presentation to CD4+ T cells. Bertolino, P., Rabourdin-Combe, C. Crit. Rev. Immunol. (1996) [Pubmed]
  24. Identification of two proteins, S14 and UIP1, that interact with UCH37. Li, T., Duan, W., Yang, H., Lee, M.K., Bte Mustafa, F., Lee, B.H., Teo, T.S. FEBS Lett. (2001) [Pubmed]
  25. Molecular evolution of the mammalian ribosomal protein gene, RPS14. Rhoads, D.D., Roufa, D.J. Mol. Biol. Evol. (1991) [Pubmed]
  26. Recombinant polypeptide from the endonuclease region of the acquired immune deficiency syndrome retrovirus polymerase (pol) gene detects serum antibodies in most infected individuals. Steimer, K.S., Higgins, K.W., Powers, M.A., Stephans, J.C., Gyenes, A., George-Nascimento, C., Luciw, P.A., Barr, P.J., Hallewell, R.A., Sanchez-Pescador, R. J. Virol. (1986) [Pubmed]
  27. Calcitonin gene-related peptide-I preferentially stimulates secretion of somatostatin from intestinal cultures. Brubaker, P.L., Greenberg, G.R. Endocrinology (1993) [Pubmed]
  28. Thrittene, homologous with somatostatin-28((1-13)), is a novel peptide in mammalian gut and circulation. Ensinck, J.W., Baskin, D.G., Vahl, T.P., Vogel, R.E., Laschansky, E.C., Francis, B.H., Hoffman, R.C., Krakover, J.D., Stamm, M.R., Low, M.J., Rubinstein, M., Otero-Corchon, V., D'Alessio, D.A. Endocrinology (2002) [Pubmed]
 
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