Disease relevance of Kit

• These data implicate Kit as one of the components leading to the Schwann cell hyperplasia observed in NF1 [1].
• Mutations in the genes encoding the Kit tyrosine kinase receptor or kit ligand (KL) cause numerous phenotypic defects, including sterility [2].

High impact information on Kit

• The Src-selective kinase inhibitor PP1 also inhibits Kit and Bcr-Abl tyrosine kinases [3].
• Flt3/Flk2 belongs to a family of receptors, including Kit and colony-stimulating factor-1R, which support growth and differentiation within the hematopoietic system [4].
• In distinction to Kit and colony-stimulating factor-1R, mutant receptors unable to couple to PI3K and expressed in rodent fibroblasts or in the interleukin 3-dependent cell line Ba/F3 provide a mitogenic signal comparable to wild-type receptors [4].
• The membrane-anchored forms of transforming growth factor-alpha (TGF-alpha) and stem cell growth factors (Kit ligands) KL-1 and KL-2 are converted to soluble growth factor forms by a regulated proteolytic cleavage process [5].
• KL mRNA and protein were detected in the OSE cell layer of rat ovaries, and primary cultures of rat OSE as well as the immortalized rat OSE cell line, ROSE 199, expressed KL, but not Kit receptors [6].

Biological context of Kit

• The purpose of this study was to determine the role KL/Kit interactions play in the regulation of oocyte meiosis [2].
• The initial parameters for the CE method were developed using the Promega Core Footprinting Kit for analysis of AP-2 binding sites in the SV40 enhancer sequence [7].
• MAIN OUTCOME MEASURES: The IEC-6 cells were stained for viability and apoptosis using trypan blue and a direct immunoperoxidase detection of digoxigenin-labeled genomic DNA (Apop Tag Plus In Situ Apoptosis Detection Kit, Oncor, Gaithersburg, Md), respectively [8].
• The analysis of peripheral blood lymphocyte was employed in in situ Cell Death Detection Kit and evaluated by flow cytometry [9].
• Cell viability was determined by Live/Dead Viability/Cytotoxicity Assay Kit, LDH release assay and MTT conversion test [10].

Anatomical context of Kit

• The expression of mostly soluble KL in the surface epithelium suggests that this cytokine may be acting in a paracrine fashion, perhaps interacting with nearby Kit receptor-bearing theca cells [6].
• High levels of Kit expression in the neurofibrosarcoma-derived Schwann cells correlate with a decrease in neurofibromin expression [1].
• In the postnatal ovary, Kit is expressed on the oocyte surface and KL is produced by the surrounding granulosa cells, but its function in these cells is still unknown [2].
• Coupling of the Kit receptor protein on gonocytes to stem cell factor from Sertoli cells is vital for successful migration by gonocytes, as antagonism of the former suppresses and addition of the latter stimulates gonocyte migration [11].
• Spontaneous phasic contractions were irregular in the distended intestinal regions of ETB(-/-) rats, and this was associated with an increased number of macrophages and damage to interstitial cells of Cajal (ICC) as revealed by using Kit-like immunoreactivity and electron microscopy [12].

Associations of Kit with chemical compounds

• Thus, KL-activated Kit inhibits meiotic progression, and the in vivo luteinizing hormone-stimulated resumption of meiosis may negate Kit activity by a localized decrease in KL expression and by altering the form of KL produced within the follicle [2].
• The RIA of the generated angiotensin I is carried out with the commercially available NEN Kit [13].
• For immunohistochemical analysis, the labeled streptavidin biotin method was performed using a Vectastain Universal Quick Kit with microwave accentuation [14].
• Two different HEDP content kits (Kit A, HEDP: 1 mg, SnCl2. 2H2O: 0.5 mg; and Kit B, HEDP: 10 mg, SnCl2. 2H2O: 0.5 mg) were evaluated for their stability against dilution 99mTc-HEDP solutions prepared from these two kits were diluted from 10 to 6000fold with 0.9% NaCl solution just before evaluation both in vivo and in vitro [15].

Analytical, diagnostic and therapeutic context of Kit

• This, together with the easy Kit preparation, makes [123I]-2-iodo-L-phenylalanine a promising tumor specific tracer for SPECT [16].
• For detection of DNA breaks (apoptosis) in the cells of the seminiferous epithelium, the testicular sections were treated by the TUNEL method (Apop-Tag Plus Peroxidase Kit) [17].
• Mucosal scrapings were extracted in 2 ml Krebs-Hemselest buffer for determination of interleukins-2, 6 and 10 using the Biosource International Immunoassay Kit [18].
• Serum concentration of the cross-linked carboxyterminal telopeptide of type I collagen was analyzed by the Rat Telopeptide [125I]ICTP Radioimmunoassay Kit obtained from Orion Diagnostica (Finland) [19].
• Western immunoblotting confirmed the low recovery of tetrameric TTR by the 2-D Clean-Up Kit and showed that the tetrameric form of TTR predominated in ovine but not in rat CSF [20].

References