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Gene Review

CHAF1B  -  chromatin assembly factor 1, subunit B (p60)

Homo sapiens

Synonyms: CAF-1, CAF-1 subunit B, CAF-I 60 kDa subunit, CAF-I p60, CAF-IP60, ...
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Disease relevance of CHAF1B

  • Using immunoblotting we determined Coxsackievirus and adenovirus receptor (CAR), Claudin-8, C21orf2, Chromatin assembly factor 1 p60 subunit (CAF-1 p60) in frontal cortex from DS, AD and control patients [1].
  • BACKGROUND AND PURPOSE: The Caf1 secretion pathway of Yersinia pestis is one of the most well-characterized export machineries [2].
  • CONCLUSION: These results suggested that the recombinant hEGF was successfully secreted through the inner membrane of cells into the periplasm and then through the outer membrane into the medium via the action of the signal peptide of Y. pestis Caf1 in E. coli [2].
  • This mapping position places CAF1P60 in a region of HSA21 which is strongly associated with the major features of Down syndrome [3].
  • Overexpression of hCAF-1 in NIH 3T3 and osteosarcoma (U-2-OS) cells was itself anti-proliferative with colony formation reduced by 67% and 90% respectively [4].

High impact information on CHAF1B

  • Chromatin assembly factor 1 (CAF-1) assembles nucleosomes in a replication-dependent manner [5].
  • Chromatin assembly factor 1 (CAF-1) represents the best biochemically characterized factor promoting chromatin assembly during DNA replication or repair in human cell-free systems [6].
  • We propose that perpetuation of heterochromatin involves self-maintenance factors, including local concentration of Hp1alpha and -beta, and that a degree of plasticity is provided by the cycle of H4 acetylation/deacetylation assisted by CAF-1 [7].
  • After UV irradiation, the chromatin-associated form of CAF-1 dramatically increased in all cells irrespective of their position in the cell cycle [8].
  • Consistent with these in vivo observations, we found that the amount of CAF-1 required to stimulate nucleosome assembly during the repair of UV photoproducts in vitro depended upon both the number of lesions and the phosphorylation state of CAF-1 [8].

Chemical compound and disease context of CHAF1B

  • Protection against virulent plague challenge by the parenteral and aerosol routes was afforded by a single administration of microencapsulated Caf1 and LcrV antigens from Yersinia pestis in BALB/c mice [9].

Biological context of CHAF1B


Anatomical context of CHAF1B


Associations of CHAF1B with chemical compounds

  • The effects of two levels of caffeine ingestion (5, CAF1, and 10, CAF2) on postexercise oxygen consumption was investigated in six untrained women aged 20.5 (SEM 0.5) years [17].
  • Identification of four families of yCCR4- and Mg2+-dependent endonuclease-related proteins in higher eukaryotes, and characterization of orthologs of yCCR4 with a conserved leucine-rich repeat essential for hCAF1/hPOP2 binding [18].
  • Strains bearing deletions in either CCR4 or CAF1/POP2, which encode components of the cytoplasmic mRNA deadenylase complex, were particularly sensitive to HU [19].
  • Cells lacking CAF-1 and RCAF are hypersensitive to DNA-damaging agents, such as methyl methanesulfonate and camptothecin, suggesting a possible defect in double-strand break (DSB) repair [20].

Analytical, diagnostic and therapeutic context of CHAF1B

  • We report the crystal structure of an ASF1a-HIRA heterodimer and a biochemical dissection of ASF1a's mutually exclusive interactions with HIRA and the p60 subunit of CAF-1 [11].
  • In 4 nonautoimmune strains (BALB/c, DBA/2, CAF1, NZW) 60 to 72% of the model complexes perfused were taken up and remained in the liver after 20 min of continuous perfusion with oxygenated Krebs-Henseleit buffer [21].
  • According to Fourier transform infrared spectroscopy (FTIR) and circular dichroism (CD) data, IL-1beta and Caf1 are typical beta-structural proteins [16].
  • The microspheres containing either Caf1 or LcrV were blended and used to immunise mice on a single occasion, by either the intra-nasal or intra-muscular route [9].
  • They could be eluted from the CAF1 cells at 56 degrees C. These results and other data that are discussed suggest that SJL resistance to immunosuppression by contrasensitizing antibodies is related to a macrophage abnormality [22].


  1. Reduction of chromatin assembly factor 1 p60 and C21orf2 protein, encoded on chromosome 21, in Down syndrome brain. Shim, K.S., Bergelson, J.M., Furuse, M., Ovod, V., Krude, T., Lubec, G. J. Neural Transm. Suppl. (2003) [Pubmed]
  2. Secretion of biologically active human epidermal growth factor from Escherichia coli using Yersinia pestis Caf1 signal peptide. Liu, Y.L., Huang, L.M., Lin, W.P., Tsai, C.C., Lin, T.S., Hu, Y.H., Chen, H.S., Han, J.M., Wang, H.J., Liu, Y.T. Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi. (2006) [Pubmed]
  3. The gene encoding the p60 subunit of chromatin assembly factor I (CAF1P60) maps to human chromosome 21q22.2, a region associated with some of the major features of Down syndrome. Katsanis, N., Fisher, E.M. Hum. Genet. (1996) [Pubmed]
  4. Human carbon catabolite repressor protein (CCR4)-associative factor 1: cloning, expression and characterization of its interaction with the B-cell translocation protein BTG1. Bogdan, J.A., Adams-Burton, C., Pedicord, D.L., Sukovich, D.A., Benfield, P.A., Corjay, M.H., Stoltenborg, J.K., Dicker, I.B. Biochem. J. (1998) [Pubmed]
  5. Nucleosome assembly by a complex of CAF-1 and acetylated histones H3/H4. Verreault, A., Kaufman, P.D., Kobayashi, R., Stillman, B. Cell (1996) [Pubmed]
  6. Dimerization of the largest subunit of chromatin assembly factor 1: importance in vitro and during Xenopus early development. Quivy, J.P., Grandi, P., Almouzni, G. EMBO J. (2001) [Pubmed]
  7. Duplication and maintenance of heterochromatin domains. Taddei, A., Roche, D., Sibarita, J.B., Turner, B.M., Almouzni, G. J. Cell Biol. (1999) [Pubmed]
  8. Recruitment of phosphorylated chromatin assembly factor 1 to chromatin after UV irradiation of human cells. Martini, E., Roche, D.M., Marheineke, K., Verreault, A., Almouzni, G. J. Cell Biol. (1998) [Pubmed]
  9. Protection against bubonic and pneumonic plague with a single dose microencapsulated sub-unit vaccine. Elvin, S.J., Eyles, J.E., Howard, K.A., Ravichandran, E., Somavarappu, S., Alpar, H.O., Williamson, E.D. Vaccine (2006) [Pubmed]
  10. Requirement of Cyclin/Cdk2 and protein phosphatase 1 activity for chromatin assembly factor 1-dependent chromatin assembly during DNA synthesis. Keller, C., Krude, T. J. Biol. Chem. (2000) [Pubmed]
  11. Structure of a human ASF1a-HIRA complex and insights into specificity of histone chaperone complex assembly. Tang, Y., Poustovoitov, M.V., Zhao, K., Garfinkel, M., Canutescu, A., Dunbrack, R., Adams, P.D., Marmorstein, R. Nat. Struct. Mol. Biol. (2006) [Pubmed]
  12. Silencing of chromatin assembly factor 1 in human cells leads to cell death and loss of chromatin assembly during DNA synthesis. Nabatiyan, A., Krude, T. Mol. Cell. Biol. (2004) [Pubmed]
  13. Nucleosome assembly activity and intracellular localization of human CAF-1 changes during the cell division cycle. Marheineke, K., Krude, T. J. Biol. Chem. (1998) [Pubmed]
  14. Identification of novel M phase phosphoproteins by expression cloning. Matsumoto-Taniura, N., Pirollet, F., Monroe, R., Gerace, L., Westendorf, J.M. Mol. Biol. Cell (1996) [Pubmed]
  15. Chromatin assembly factor 1 is essential and couples chromatin assembly to DNA replication in vivo. Hoek, M., Stillman, B. Proc. Natl. Acad. Sci. U.S.A. (2003) [Pubmed]
  16. Structural and functional similarity between Yersinia pestis capsular protein Caf1 and human interleukin-1 beta. Abramov, V.M., Vasiliev, A.M., Vasilenko, R.N., Kulikova, N.L., Kosarev, I.V., Khlebnikov, V.S., Ishchenko, A.T., MacIntyre, S., Gillespie, J.R., Khurana, R., Korpela, T., Fink, A.L., Uversky, V.N. Biochemistry (2001) [Pubmed]
  17. The effects of two levels of caffeine ingestion on excess postexercise oxygen consumption in untrained women. Donelly, K., McNaughton, L. European journal of applied physiology and occupational physiology. (1992) [Pubmed]
  18. Identification of four families of yCCR4- and Mg2+-dependent endonuclease-related proteins in higher eukaryotes, and characterization of orthologs of yCCR4 with a conserved leucine-rich repeat essential for hCAF1/hPOP2 binding. Dupressoir, A., Morel, A.P., Barbot, W., Loireau, M.P., Corbo, L., Heidmann, T. BMC Genomics (2001) [Pubmed]
  19. Ccr4 contributes to tolerance of replication stress through control of CRT1 mRNA poly(A) tail length. Woolstencroft, R.N., Beilharz, T.H., Cook, M.A., Preiss, T., Durocher, D., Tyers, M. J. Cell. Sci. (2006) [Pubmed]
  20. Reduction of nucleosome assembly during new DNA synthesis impairs both major pathways of double-strand break repair. Lewis, L.K., Karthikeyan, G., Cassiano, J., Resnick, M.A. Nucleic Acids Res. (2005) [Pubmed]
  21. An abnormality of immune complex kinetics in murine lupus. Magilavy, D.B., Rifai, A., Plotz, P.H. J. Immunol. (1981) [Pubmed]
  22. Resistance of SJL mice to immunosuppression by antibodies. Crowle, A.J., Jacobson, S., May, M. Immunol. Commun. (1978) [Pubmed]
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