Disease relevance of Trpv6

• Calcium transport and translocation of adenine nucleotides in mitochondria from Morris hepatoma 3924A [1].
• Calcium transport in erythrocytes of rats with spontaneous hypertension [2].
• Calcium transport by plasma membranes from a glucose-responsive rat insulinoma [3].
• 1 Calcium transport activity of isolated cardiac sarcoplasmic reticulum (SR) including Ca2+ uptake and release is decreased in animals with chronic heart failure (CHF) following myocardial infarction [4].
• Calcium transport by fragmented sarcoplasmic reticulum was also depressed in the presence and absence of oxalate in acute uremia [5].

High impact information on Trpv6

• Calcium transport abnormality in uremic rat brain synaptosomes [6].
• Calcium transport of Plasmodium chabaudi-infected erythrocytes [7].
• Calcium transport in small intestine of rat and chick has been studied at the cellular level using the electron probe X-ray microanalyzer [8].
• In situ hybridization revealed strong CaT1 mRNA expression in enterocytes of duodenum, proximal jejunum, and cecum [9].
• Here we report the properties of a calcium transport protein (CaT1) cloned from rat duodenum using an expression cloning strategy in Xenopus laevis oocytes, which likely plays a key role in the intestinal uptake of calcium [9].

Biological context of Trpv6

• Uterine CaT1 mRNA was highly expressed at diestrus compared with proestrus, whereas PMCA1b expression was not altered during the estrus cycle [10].
• Thus, in the present study, the expression patterns of CaT1 and PMCA1b were examined to predict their roles in rat uterus during the estrous cycle [10].
• Calcium transport in the intestine increases late in pregnancy, peaks during lactation, and then falls to control values by 3 wk postweaning in both vitamin D-replete and D-deficient animals [11].
• Calcium transport in dentinogenesis. An experimental study in the rat incisor odontoblast [12].

Anatomical context of Trpv6

• Our studies strongly suggest that CaT1 provides the principal mechanism for Ca(2+) entry into enterocytes as part of the transcellular pathway of calcium absorption in the intestine [9].
• When expressed in Xenopus oocytes, CaT1 mediates saturable Ca(2+) uptake with a Michaelis constant of 0.44 mM [9].
• Calcium-related proteins include the calcium transporters 1 and 2 (CaT1 and CaT2), plasma membrane Ca2+-ATPase 1b (PMCA1b), and calbindin-D9k and -D28k [10].
• Both CaT1 and PMCA1b mRNAs were detected in rat uterus [10].
• In addition, CaT1 mRNA was expressed in uterine endometrium and glandular endometrium at diestrus in P4-treated rats [10].

Associations of Trpv6 with chemical compounds

• Fe(3+), Fe(2+), Mn(2+), and Zn(2+) have no significant effects at 100 microM on CaT1-mediated Ca(2+) transport [9].
• CaT1 is also permeant to Sr(2+) and Ba(2+) (but not Mg(2+)), although the currents evoked by Sr(2+) and Ba(2+) are much smaller than those evoked by Ca(2+) [9].
• The trivalent cations Gd(3+) and La(3+) and the divalent cations Cu(2+), Pb(2+), Cd(2+), Co(2+), and Ni(2+) (each at 100 microM) do not evoke currents themselves, but inhibit CaT1-mediated Ca(2+) transport [9].
• To evaluate the sex steroids involved in uterine CaT1 mRNA regulation, 17beta-estradiol (E2) and/or progesterone (P4) were injected into immature rats [10].
• Calcium transport, however, was inhibited by vanadate in a concentration-dependent fashion with a K0.5 of 10 microM [13].

Regulatory relationships of Trpv6

• Together, these results suggest that CaT1 is regulated by P4 at diestrus via a PR-dependent pathway [10].
• Calcium transport by the Ca2(+)-pumping ATPase in rat duodenal basolateral-enriched membrane vesicles was stimulated by synthetic calbindin-D9k in a similar fashion to the purified natural protein [14].

Other interactions of Trpv6

• Calcium transport by sarcoplasmic reticulum of vascular smooth muscle: II. Effects of calmodulin and calmodulin inhibitors [15].
• Calcium transport in isolated bone cells. III. Effects of parathyroid hormone and cyclic 3',5'-AMP [16].

Analytical, diagnostic and therapeutic context of Trpv6

• Calcium transport by the Na+/Ca2+ exchanger was measured in plasma membranes vesicles purified from rat brain and in primary rat cortical cell culture [17].
• Northern-blot analysis of intestinal ribonucleic acid of vitamin D-deficient rats given seven daily injections of vehicle or 100 ng 1,25(OH)(2)D(3) or 19-norD(2) revealed that 19-norD(2) was less potent than 1,25(OH)(2)D(3) in stimulating expression of CaT1, Calbindin D9k and PMCA1 [18].

References