Disease relevance of Dentinogenesis

• Although the role of dentin molecules in dentinogenesis is well known, their role in pathological processes associated with dentin matrix dissolution is unclear [1].
• These data reveal that a single application of up to 50 microg/tooth of exogenous recombinant BMP-7 is insufficient to induce reparative dentinogenesis in ferret teeth with reversible pulpitis [2].
• These results confirm that the effects observed are probably due to a PTH deficiency and/or hypocalcemia and suggest that their occurrence is associated with a determined stage of dentinogenesis in the rat [3].
• Previous studies show that a high sucrose diet reduces the rate of primary dentinogenesis and increases dental caries, although their cause-effect relationship is still obscure [4].
• This article addresses treatment of patients with various types of congenital defects, including partial and total anodontia, hypohydrotic ectodermal dysplasia, dentinogenesis imperfecta, and cleft lip and palate [5].

High impact information on Dentinogenesis

• DSPP mutation in dentinogenesis imperfecta Shields type II [6].
• The Dmp1 gene was originally identified from a rat incisor cDNA library and has been shown to play an important role in late stage dentinogenesis [7].
• To address the role of DMP-1 in tooth during postnatal development, we analyzed changes of dentinogenesis in Dmp-1 null mice from 3 days after birth to 1 year [8].
• We envision that the proteolytic processing of DMP1 plays a crucial role during osteogenesis and dentinogenesis [9].
• Most importantly, the expression of dentin sialophosphoprotein (dspp), a candidate gene implicated in dentinogenesis imperfecta II (MIM 125420), is significantly down-regulated in the transgenic teeth [10].

Biological context of Dentinogenesis

• The tooth phenotype of these mice is strikingly similar to that in dentin sialophosphoprotein (Dspp) null mice and shares some features of the human disease dentinogenesis imperfecta III [8].
• These findings also provide for the first time a direct experimental evidence indicating that decreased dspp gene expression along with the other cellular changes in odontoblasts may result in human hereditary dental disorders like dentinogenesis imperfecta II (MIM 125420) and dentin dysplasia (MIM 125400 and 125420) [10].
• The expression of osteonectin during dentinogenesis and cementogenesis is closely related to the development of the respective calcified tissue [11].
• These findings suggest physiological roles of Osc, Osn, and Opn in bone remodeling, PDL remodeling, dentinogenesis, and cementogenesis [12].
• Significantly, the DSP/DPP gene has been localized to human chromosome 4q21 at a site implicated in dentinogenesis imperfecta type II [13].

Anatomical context of Dentinogenesis

• We identified a nonsense mutation (Gln45stop) in exon 3 of the dentin sialophosphoprotein (DSPP) gene in a Chinese family with dentinogenesis imperfecta Shields type II (DGI-II), in which the affected members showed discoloration and severe attrition of their teeth, with obliterated pulp chambers [6].
• As recent studies have shown that dentinogenesis imperfecta type II results from mutation of the bicistronic dentine sialophosphoprotein gene (DSPP ), we have tested this hypothesis by sequencing DSPP in a family with a history of dentine dysplasia type II [14].
• Growth-hormone-stimulated dentinogenesis in Lewis dwarf rat molars [15].
• We have examined rat molar tooth germs by applying a high-resolution immunocytochemical approach with the purpose to identify the temporal and spatial localization of DMP 1 at the onset of dentinogenesis [16].
• Inability of calcium hydroxide to induce reparative dentinogenesis at non-peripheral sites of dog dental pulp [17].

Associations of Dentinogenesis with chemical compounds

• The findings confirm that sucrose-diet-induced reduction in dentinogenesis is independent of insulin and loss of minerals in urine [18].
• Immunolocalization of fibronectin during reparative dentinogenesis in human teeth after pulp capping with calcium hydroxide [19].
• With regard to BM components, inducible dentinogenesis was Arg-Gly-Asp (RGD)-dependent [20].
• The orientation of the fibres was at right angles to the future amelo-dentinal junction in coronal dentinogenesis, but parallel to the root surface in radicular dentinogenesis [21].
• In conclusion, dietary xylitol reduces dentin formation in young adult rats in spite of the slow rate of dentinogenesis [22].

Gene context of Dentinogenesis

• These two domains and the colocalization of Dmp1 and Dmp2 genes at a position equivalent to the dentinogenesis imperfecta type II location on human 4q21 all suggest that the PPs are indeed involved in some aspect of ECM mineralization [23].
• A novel splice acceptor mutation in the DSPP gene causing dentinogenesis imperfecta type II [24].
• We envision that the proteolytic processing of DMP1 and DSPP may be an activation process that plays a significant, crucial role in osteogenesis and dentinogenesis, and that a failure in this processing would cause defective mineralization in bone and dentin, as observed in X-linked hypophosphatemic rickets [25].
• Recent evidence has implicated proteins belonging to the bone morphogenetic protein (BMP) subgroup of the transforming growth factor beta supergene family in tooth formation and dentinogenesis [26].
• We furthermore examined dentinogenesis in tooth explants cultured in the presence of warfarin (an inhibitor of gamma-carboxylation of OC) [27].

Analytical, diagnostic and therapeutic context of Dentinogenesis

• Immunohistochemistry of extracellular matrix proteins during various stages of dentinogenesis [28].
• Synthesizing the present data with previous relevant information it could be suggested that in the mechanism initiating reparative dentinogenesis, growth factors (endogenous or artificially implanted) and fibronectin are involved and this mechanism seems to be more complex than the simple immobilization of pulp cells onto an adhesion substratum [29].
• In order to determine the differential effects of the thyroid hormones and the parathyroid hormone upon dentinogenesis in the rat incisor one control group (C) and four groups of surgically treated rats were studied: parathyroid autotransplanted (PTT), thyroidectomized (TX), parathyroidectomized (PTX), and thyro-parathyroidectomized group [3].
• In vitro characterization of poly(ethylene) glycol calcium citrate microspheres as a delivery system for the study of reparative dentinogenesis [30].

References