The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

HSPB1  -  heat shock 27kDa protein 1

Homo sapiens

Synonyms: 28 kDa heat shock protein, CMT2F, Estrogen-regulated 24 kDa protein, HEL-S-102, HMN2B, ...
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of HSPB1


Psychiatry related information on HSPB1


High impact information on HSPB1

  • Coimmunoprecipitation experiments showed greater binding of both HSPB8 mutants to the interacting partner HSPB1 [6].
  • Cotransfection of neurofilament light chain (NEFL) and mutant HSPB1 resulted in altered neurofilament assembly in cells devoid of cytoplasmic intermediate filaments [7].
  • Screening for mutations in HSPB1 in 301 individuals with CMT and 115 individuals with distal hereditary motor neuropathies (distal HMNs) confirmed the previously observed mutation and identified four additional missense mutations [7].
  • In the present study, the effect of altered expression of hsp70 and low molecular weight heat-shock protein, hsp27, on tumor cell sensitivity to monocytes and lymphokine-activated killer (LAK) cells was studied [8].
  • Surprisingly, overexpression of human hsp27, which does not protect WEHI-S cells from TNF killing, conferred partial resistance to monocytes [8].

Chemical compound and disease context of HSPB1


Biological context of HSPB1

  • A mutation in the small heat-shock protein HSPB1 leading to distal hereditary motor neuronopathy disrupts neurofilament assembly and the axonal transport of specific cellular cargoes [14].
  • An in-gel kinase assay demonstrated that PRAK is a major stress-activated kinase that can phosphorylate small heat shock protein, suggesting a potential role for PRAK in mediating stress-induced HSP27 phosphorylation in vivo [15].
  • These data suggest a role for HSP27 in the signal transduction events of platelet activation [16].
  • Moreover, AS-Hsp27 overcomes interleukin-6 (IL-6)-mediated protection against Dex-induced apoptosis [1].
  • Amino acid sequences of peptides formed by cleavage of the purified 28-kDa protein and alpha B crystallin were identical to those of particular regions of the deduced amino acid sequences of human small heat shock protein (HSP28) and lens alpha B crystallin, respectively [17].

Anatomical context of HSPB1


Associations of HSPB1 with chemical compounds

  • Using this antibody, we isolated and sequenced a human cDNA clone encoding a protein that was identical to the mammalian 27-kDa heat shock protein (HSP27), a protein of uncertain function that is known to be phosphorylated to several forms and to be transcriptionally induced by estrogen [16].
  • Furthermore, the removal capacities of the two major types of UVC-damaged DNA (thymine dimers and (6-4)photoproducts) in the cells with the up-regulation of HSP27 were moderately elevated compared with those in the control cells, while those in the cells with down-regulation were remarkably suppressed [21].
  • Hypo-osmotic swelling of human Intestine 407 cells leads to a significant increase of intracellular MAPKAP-kinase 2 activity and Hsp27 phosphorylation [22].
  • RESULTS: HSP27 overexpression induced cellular resistance to heat shock at 45 degrees C for 1 h as well as against several cytotoxic agents, including cisplatin, staurosporin and H(2)O(2) [23].
  • The protein kinase inhibitor SB203580 uncouples PMA-induced differentiation of HL-60 cells from phosphorylation of Hsp27 [24].

Physical interactions of HSPB1


Enzymatic interactions of HSPB1

  • Activated PRAK in turn phosphorylated small heat shock protein 27 (HSP27) at the physiologically relevant sites [15].

Regulatory relationships of HSPB1


Other interactions of HSPB1

  • Based on the MAPKAP kinase 2 phosphorylation site of Hsp27, a competitive inhibitory peptide was synthesized [31].
  • These data demonstrate that Hsp27 inhibits the release of Smac, and thereby confers Dex resistance in MM cells [1].
  • In contrast, the other sHsp Hsp27/HspB1 and alphaB-crystallin/HspB5 had no effect [32].
  • The induction of HSP27 by stress exposure was dramatically suppressed in the infected cells, but HSP70 induction was not affected [2].
  • Suppression of thermotolerance in mumps virus-infected cells is caused by lack of HSP27 induction contributed by STAT-1 [2].

Analytical, diagnostic and therapeutic context of HSPB1


  1. Hsp27 inhibits release of mitochondrial protein Smac in multiple myeloma cells and confers dexamethasone resistance. Chauhan, D., Li, G., Hideshima, T., Podar, K., Mitsiades, C., Mitsiades, N., Catley, L., Tai, Y.T., Hayashi, T., Shringarpure, R., Burger, R., Munshi, N., Ohtake, Y., Saxena, S., Anderson, K.C. Blood (2003) [Pubmed]
  2. Suppression of thermotolerance in mumps virus-infected cells is caused by lack of HSP27 induction contributed by STAT-1. Yokota, S., Yokosawa, N., Kubota, T., Okabayashi, T., Arata, S., Fujii, N. J. Biol. Chem. (2003) [Pubmed]
  3. Ectopic expression of Hsp70 confers resistance and silencing its expression sensitizes human colon cancer cells to curcumin-induced apoptosis. Rashmi, R., Kumar, S., Karunagaran, D. Carcinogenesis (2004) [Pubmed]
  4. Differential expression of the MAD2, BUB1 and HSP27 genes in Barrett's oesophagus-their association with aneuploidy and neoplastic progression. Doak, S.H., Jenkins, G.J., Parry, E.M., Griffiths, A.P., Baxter, J.N., Parry, J.M. Mutat. Res. (2004) [Pubmed]
  5. Alpha B crystallin and HSP28 are enhanced in the cerebral cortex of patients with Alzheimer's disease. Shinohara, H., Inaguma, Y., Goto, S., Inagaki, T., Kato, K. J. Neurol. Sci. (1993) [Pubmed]
  6. Hot-spot residue in small heat-shock protein 22 causes distal motor neuropathy. Irobi, J., Van Impe, K., Seeman, P., Jordanova, A., Dierick, I., Verpoorten, N., Michalik, A., De Vriendt, E., Jacobs, A., Van Gerwen, V., Vennekens, K., Mazanec, R., Tournev, I., Hilton-Jones, D., Talbot, K., Kremensky, I., Van Den Bosch, L., Robberecht, W., Van Vandekerckhove, J., Broeckhoven, C., Gettemans, J., De Jonghe, P., Timmerman, V. Nat. Genet. (2004) [Pubmed]
  7. Mutant small heat-shock protein 27 causes axonal Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. Evgrafov, O.V., Mersiyanova, I., Irobi, J., Van Den Bosch, L., Dierick, I., Leung, C.L., Schagina, O., Verpoorten, N., Van Impe, K., Fedotov, V., Dadali, E., Auer-Grumbach, M., Windpassinger, C., Wagner, K., Mitrovic, Z., Hilton-Jones, D., Talbot, K., Martin, J.J., Vasserman, N., Tverskaya, S., Polyakov, A., Liem, R.K., Gettemans, J., Robberecht, W., De Jonghe, P., Timmerman, V. Nat. Genet. (2004) [Pubmed]
  8. Heat-shock proteins protect cells from monocyte cytotoxicity: possible mechanism of self-protection. Jäättelä, M., Wissing, D. J. Exp. Med. (1993) [Pubmed]
  9. Modulation of estrogen signaling by the novel interaction of heat shock protein 27, a biomarker for atherosclerosis, and estrogen receptor beta: mechanistic insight into the vascular effects of estrogens. Miller, H., Poon, S., Hibbert, B., Rayner, K., Chen, Y.X., O'Brien, E.R. Arterioscler. Thromb. Vasc. Biol. (2005) [Pubmed]
  10. Expression of heat shock protein 27 in human renal cell carcinoma. Sarto, C., Valsecchi, C., Magni, F., Tremolada, L., Arizzi, C., Cordani, N., Casellato, S., Doro, G., Favini, P., Perego, R.A., Raimondo, F., Ferrero, S., Mocarelli, P., Galli-Kienle, M. Proteomics (2004) [Pubmed]
  11. Increased Hsp27 after androgen ablation facilitates androgen-independent progression in prostate cancer via signal transducers and activators of transcription 3-mediated suppression of apoptosis. Rocchi, P., Beraldi, E., Ettinger, S., Fazli, L., Vessella, R.L., Nelson, C., Gleave, M. Cancer Res. (2005) [Pubmed]
  12. Heat shock proteins hsp27 and hsp70: lack of correlation with response to tamoxifen and clinical course of disease in estrogen receptor-positive metastatic breast cancer (a Southwest Oncology Group Study). Ciocca, D.R., Green, S., Elledge, R.M., Clark, G.M., Pugh, R., Ravdin, P., Lew, D., Martino, S., Osborne, C.K. Clin. Cancer Res. (1998) [Pubmed]
  13. Heat shock proteins and drug resistance. Fuqua, S.A., Oesterreich, S., Hilsenbeck, S.G., Von Hoff, D.D., Eckardt, J., Osborne, C.K. Breast Cancer Res. Treat. (1994) [Pubmed]
  14. A mutation in the small heat-shock protein HSPB1 leading to distal hereditary motor neuronopathy disrupts neurofilament assembly and the axonal transport of specific cellular cargoes. Ackerley, S., James, P.A., Kalli, A., French, S., Davies, K.E., Talbot, K. Hum. Mol. Genet. (2006) [Pubmed]
  15. PRAK, a novel protein kinase regulated by the p38 MAP kinase. New, L., Jiang, Y., Zhao, M., Liu, K., Zhu, W., Flood, L.J., Kato, Y., Parry, G.C., Han, J. EMBO J. (1998) [Pubmed]
  16. The 29-kDa proteins phosphorylated in thrombin-activated human platelets are forms of the estrogen receptor-related 27-kDa heat shock protein. Mendelsohn, M.E., Zhu, Y., O'Neill, S. Proc. Natl. Acad. Sci. U.S.A. (1991) [Pubmed]
  17. Copurification of small heat shock protein with alpha B crystallin from human skeletal muscle. Kato, K., Shinohara, H., Goto, S., Inaguma, Y., Morishita, R., Asano, T. J. Biol. Chem. (1992) [Pubmed]
  18. Ischemic preconditioning: from adenosine receptor to KATP channel. Cohen, M.V., Baines, C.P., Downey, J.M. Annu. Rev. Physiol. (2000) [Pubmed]
  19. Heat shock protein 27 controls apoptosis by regulating Akt activation. Rane, M.J., Pan, Y., Singh, S., Powell, D.W., Wu, R., Cummins, T., Chen, Q., McLeish, K.R., Klein, J.B. J. Biol. Chem. (2003) [Pubmed]
  20. Arsenite pre-conditioning reduces UVB-induced apoptosis in corneal epithelial cells through the anti-apoptotic activity of 27 kDa heat shock protein (HSP27). Shi, B., Isseroff, R.R. J. Cell. Physiol. (2006) [Pubmed]
  21. Protective role of HSP27 against UVC-induced cell death in human cells. Wano, C., Kita, K., Takahashi, S., Sugaya, S., Hino, M., Hosoya, H., Suzuki, N. Exp. Cell Res. (2004) [Pubmed]
  22. Hypo-osmotic cell swelling activates the p38 MAP kinase signalling cascade. Tilly, B.C., Gaestel, M., Engel, K., Edixhoven, M.J., de Jonge, H.R. FEBS Lett. (1996) [Pubmed]
  23. Overexpression of human 27 kDa heat shock protein in laryngeal cancer cells confers chemoresistance associated with cell growth delay. Lee, J.H., Sun, D., Cho, K.J., Kim, M.S., Hong, M.H., Kim, I.K., Lee, J.S., Lee, J.H. J. Cancer Res. Clin. Oncol. (2007) [Pubmed]
  24. The protein kinase inhibitor SB203580 uncouples PMA-induced differentiation of HL-60 cells from phosphorylation of Hsp27. Schultz, H., Rogalla, T., Engel, K., Lee, J.C., Gaestel, M. Cell Stress Chaperones (1997) [Pubmed]
  25. Suppression of NF-kappaB activation by Entamoeba histolytica in intestinal epithelial cells is mediated by heat shock protein 27. Kammanadiminti, S.J., Chadee, K. J. Biol. Chem. (2006) [Pubmed]
  26. The 28-kDa protein whose phosphorylation is induced by protein kinase C activators in MCF-7 cells belongs to the family of low molecular mass heat shock proteins and is the estrogen-regulated 24-kDa protein. Faucher, C., Capdevielle, J., Canal, I., Ferrara, P., Mazarguil, H., McGuire, W.L., Darbon, J.M. J. Biol. Chem. (1993) [Pubmed]
  27. Hsp27 enhances recovery of splicing as well as rephosphorylation of SRp38 after heat shock. Marin-Vinader, L., Shin, C., Onnekink, C., Manley, J.L., Lubsen, N.H. Mol. Biol. Cell (2006) [Pubmed]
  28. Involvement of c-Jun NH2-terminal kinase-1 in heat-induced apoptotic cell death of human monoblastic leukaemia U937 cells. Enomoto, A., Suzuki, N., Liu, C., Kang, Y., Zhu, J., Serizawa, S., Matsumoto, Y., Morita, A., Ito, M., Hosoi, Y. Int. J. Radiat. Biol. (2001) [Pubmed]
  29. Changes in the phosphorylation status of the 27 kDa heat shock protein (HSP27) associated with the modulation of growth and/or differentiation in MCF-7 cells. Horman, S., Galand, P., Mosselmans, R., Legros, N., Leclercq, G., Mairesse, N. Cell Prolif. (1997) [Pubmed]
  30. Interleukin-1-induced intracellular signaling pathways converge in the activation of mitogen-activated protein kinase and mitogen-activated protein kinase-activated protein kinase 2 and the subsequent phosphorylation of the 27-kilodalton heat shock protein in monocytic cells. Ahlers, A., Belka, C., Gaestel, M., Lamping, N., Sott, C., Herrmann, F., Brach, M.A. Mol. Pharmacol. (1994) [Pubmed]
  31. Activation of MAP kinase-activated protein kinase 2 in human neutrophils after phorbol ester or fMLP peptide stimulation. Zu, Y.L., Ai, Y., Gilchrist, A., Labadia, M.E., Sha'afi, R.I., Huang, C.K. Blood (1996) [Pubmed]
  32. HspB8, a small heat shock protein mutated in human neuromuscular disorders, has in vivo chaperone activity in cultured cells. Carra, S., Sivilotti, M., Chávez Zobel, A.T., Lambert, H., Landry, J. Hum. Mol. Genet. (2005) [Pubmed]
  33. Decreased expression of Hsp27 and Hsp70 in transformed lymphoblastoid cells from patients with spinocerebellar ataxia type 7. Tsai, H.F., Lin, S.J., Li, C., Hsieh, M. Biochem. Biophys. Res. Commun. (2005) [Pubmed]
  34. Small heat shock protein 27 (HSP27) associates with tubulin/microtubules in HeLa cells. Hino, M., Kurogi, K., Okubo, M.A., Murata-Hori, M., Hosoya, H. Biochem. Biophys. Res. Commun. (2000) [Pubmed]
  35. TNF-alpha decreases hsp 27 in human blood mononuclear cells: Involvement of protein kinase c. Niwa, M., Hotta, K., Hara, A., Hirade, K., Ito, H., Kato, K., Kozawa, O. Life Sci. (2006) [Pubmed]
WikiGenes - Universities