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Gene Review

Hspb1  -  heat shock protein B1

Rattus norvegicus

Synonyms: HSP 27, Heat shock 27 kDa protein, Heat shock protein beta-1, Hsp25, Hsp27, ...
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Disease relevance of Hspb1

  • Hypoxia strongly induced HO-1 mRNA without affecting HSP27 mRNA [1].
  • The present study investigates the expression patterns of Hsp27 and Hsp32 in the adult rat hippocampus following whole body hyperthermia [2].
  • These observations also suggest that STAT-5B/STAT-3/Hsp27/FGF-2 signaling via its involvement in the regulation of VSMC growth and motility may play an important role in the pathogenesis of vascular diseases such as restenosis after angioplasty [3].
  • Alexander disease is a fatal neurological illness characterized by white-matter degeneration and the formation of astrocytic cytoplasmic inclusions called Rosenthal fibers, which contain the intermediate filament glial fibrillary acidic protein (GFAP), the small heat-shock proteins HSP27 and alphaB-crystallin, and ubiquitin [4].
  • CONCLUSIONS: OFF phenomenon of thrombin-induced tolerance of the brain to edema formation may be related to HSP27 induction [5].

Psychiatry related information on Hspb1


High impact information on Hspb1


Chemical compound and disease context of Hspb1

  • Sphingosine 1-phosphate significantly induced the accumulation of HSP27 in a pertussis toxin-sensitive manner [9].
  • The levels of Hsp27 and alphaB-crystallin in C6 rat glioma cells, that had been heated at 43 degrees C for 30 min with a subsequent culture for 16 h at 37 degrees C, were markedly increased [10].
  • The exposure of the cells to a low concentration (0.1-3 microg/ml) of anisomycin for a few hours after heat stress stimulated the accumulation of the small stress proteins Hsp27 and alphaB-crystallin, but not that of Hsp70 [10].
  • These findings suggest that the induction of HO-1 and HSP27 during withdrawal from phenobarbital dependence may play a role in protection against glutamate toxicity [11].
  • After ischemia untreated livers showed a translocation of Hsp27 towards the cytoskeleton and an increase in total Hsp27, whereas ANP preconditioning prohibited translocation but caused an augmentation of Hsp27 phosphorylation [12].

Biological context of Hspb1


Anatomical context of Hspb1


Associations of Hspb1 with chemical compounds

  • S-thiolation must occur at Cys-141, the only cysteine in rat HSP27 [21].
  • SB-203580 reduced the thrombin-increased level of HSP27 mRNA [20].
  • Thrombin stimulated the accumulation of HSP27 dose dependently between 0.01 and 1 U/ml and cycloheximide reduced the accumulation [20].
  • Thrombin stimulated an increase in the level of HSP27 mRNA and actinomycin D suppressed the thrombin-increased mRNA level [20].
  • Levels and phosphorylation states of the two small molecular chaperones, alphaB-crystallin and Hsp27, in disused rat soleus muscles were determined by Western blot analysis of extracts with antibodies recognizing each of the two proteins and their phosphorylated serine residues [22].

Physical interactions of Hspb1


Enzymatic interactions of Hspb1


Regulatory relationships of Hspb1

  • The results of these experiments show that the p38 MAPK pathway is activated in GC in response to FSH in a cAMP/PKA-dependent manner, and that p38 MAPK activity is required for FSH-induced HSP-27 phosphorylation as well as rounding/aggregation in GC [24].
  • Therefore, PC that results in brain tolerance/neuroprotection increases neuronal Hsp70 in the PC cortex and activated astrocytic Hsp27 in the PC cortex in a temporal fashion associated with developing IT [25].
  • In cultured aortic smooth muscle A10 cells, AVP markedly stimulated the phosphorylation of HSP27 at Ser-15 and Ser-85 [26].
  • Our results suggest that ATF3- and c-Jun-induced Hsp27 expression is a novel survival response in neurons under death stress such as nerve injury [27].
  • CONCLUSIONS: hsp27 phosphorylation was stimulated by low and high concentrations of cholecystokinin, both in vivo and in vitro [28].

Other interactions of Hspb1


Analytical, diagnostic and therapeutic context of Hspb1

  • S-thiolated proteins were purified using streptavidin-agarose, and Western immunoblotting showed HSP27 was present [21].
  • HSP27 mRNA and protein levels were determined using semiquantitative polymerase chain reaction, microarray (stress-toxicity, GEArray) analyses, gene sequencing, immunoblotting, and immunohistochemical analyses at 10 days and 6 months posttransplant [32].
  • The authors hypothesized that HSP27 is involved in chronic allograft nephropathy (CAN), a chronic state of inflammation and stress [32].
  • The cDNA was used in Northern blot analysis to estimate the relative levels of hsp27 mRNA in rat seminiferous tubule segments selected for different stages of the cycle of the seminiferous epithelium [30].
  • In addition, the small heat shock protein Hsp27, with its anti-apoptotic effects, may be important for neuron survival following axotomy or trophic factor withdrawal [13].


  1. Hypoxia differentially regulates stress proteins in cultured cardiomyocytes: role of the p38 stress-activated kinase signaling cascade, and relation to cytoprotection. Kacimi, R., Chentoufi, J., Honbo, N., Long, C.S., Karliner, J.S. Cardiovasc. Res. (2000) [Pubmed]
  2. Induction of Hsp27 and Hsp32 stress proteins and vimentin in glial cells of the rat hippocampus following hyperthermia. Bechtold, D.A., Brown, I.R. Neurochem. Res. (2003) [Pubmed]
  3. Novel role for STAT-5B in the regulation of Hsp27-FGF-2 axis facilitating thrombin-induced vascular smooth muscle cell growth and motility. Cao, H., Dronadula, N., Rizvi, F., Li, Q., Srivastava, K., Gerthoffer, W.T., Rao, G.N. Circ. Res. (2006) [Pubmed]
  4. Alexander-disease mutation of GFAP causes filament disorganization and decreased solubility of GFAP. Hsiao, V.C., Tian, R., Long, H., Der Perng, M., Brenner, M., Quinlan, R.A., Goldman, J.E. J. Cell. Sci. (2005) [Pubmed]
  5. Attenuation of thrombin-induced brain edema by cerebral thrombin preconditioning. Xi, G., Keep, R.F., Hua, Y., Xiang, J., Hoff, J.T. Stroke (1999) [Pubmed]
  6. Expression of Hsp27 in retinal ganglion cells of the rat during postnatal development. Hawkes, E.L., Krueger-Naug, A.M., Nickerson, P.E., Myers, T.L., Currie, R.W., Clarke, D.B. J. Comp. Neurol. (2004) [Pubmed]
  7. Hsp27 upregulation and phosphorylation is required for injured sensory and motor neuron survival. Benn, S.C., Perrelet, D., Kato, A.C., Scholz, J., Decosterd, I., Mannion, R.J., Bakowska, J.C., Woolf, C.J. Neuron (2002) [Pubmed]
  8. In vivo regulation of MAP kinases in Ratus norvegicus renal papilla by water loading and restriction. Wojtaszek, P.A., Heasley, L.E., Berl, T. J. Clin. Invest. (1998) [Pubmed]
  9. Sphingosine 1-phosphate regulates heat shock protein 27 induction by a p38 MAP kinase-dependent mechanism in aortic smooth muscle cells. Kozawa, O., Tanabe, K., Ito, H., Matsuno, H., Niwa, M., Kato, K., Uematsu, T. Exp. Cell Res. (1999) [Pubmed]
  10. Selective stimulation of Hsp27 and alphaB-crystallin but not Hsp70 expression by p38 MAP kinase activation. Kato, K., Ito, H., Kamei, K., Iwamoto, I. Cell Stress Chaperones (1999) [Pubmed]
  11. Brain responses to acute withdrawal in phenobarbital-dependent rats. Tanaka, S., Okuno, Y., Numazawa, S., Yamamoto, T., Shioda, S., Yoshida, T. Eur. J. Pharmacol. (2001) [Pubmed]
  12. Hepatocyte cytoskeleton during ischemia and reperfusion--influence of ANP-mediated p38 MAPK activation. Keller, M., Gerbes, A.L., Kulhanek-Heinze, S., Gerwig, T., Grutzner, U., van Rooijen, N., Vollmar, A.M., Kiemer, A.K. World J. Gastroenterol. (2005) [Pubmed]
  13. Administration of brain-derived neurotrophic factor suppresses the expression of heat shock protein 27 in rat retinal ganglion cells following axotomy. Krueger-Naug, A.M., Emsley, J.G., Myers, T.L., Currie, R.W., Clarke, D.B. Neuroscience (2003) [Pubmed]
  14. Possible involvement of phosphatidylinositol 3-kinase/Akt signal pathway in vasopressin-induced HSP27 phosphorylation in aortic smooth muscle A10 cells. Suga, H., Nakajima, K., Shu, E., Kanno, Y., Hirade, K., Ishisaki, A., Matsuno, H., Tanabe, K., Takai, S., Akamatsu, S., Kato, K., Oiso, Y., Kozawa, O. Arch. Biochem. Biophys. (2005) [Pubmed]
  15. Stress-induced heat shock protein 27 expression and its role in dorsal root ganglion neuronal survival. Dodge, M.E., Wang, J., Guy, C., Rankin, S., Rahimtula, M., Mearow, K.M. Brain Res. (2006) [Pubmed]
  16. Stress-mediated signaling in PC12 cells - the role of the small heat shock protein, Hsp27, and Akt in protecting cells from heat stress and nerve growth factor withdrawal. Mearow, K.M., Dodge, M.E., Rahimtula, M., Yegappan, C. J. Neurochem. (2002) [Pubmed]
  17. p38 mitogen-activated protein kinase protects glomerular epithelial cells from complement-mediated cell injury. Aoudjit, L., Stanciu, M., Li, H., Lemay, S., Takano, T. Am. J. Physiol. Renal Physiol. (2003) [Pubmed]
  18. Induction of heat shock proteins (HSPs) by sodium arsenite in cultured astrocytes and reduction of hydrogen peroxide-induced cell death. Fauconneau, B., Petegnief, V., Sanfeliu, C., Piriou, A., Planas, A.M. J. Neurochem. (2002) [Pubmed]
  19. HSP27 is markedly induced in Schwann cell columns and associated regenerating axons. Hirata, K., He, J., Hirakawa, Y., Liu, W., Wang, S., Kawabuchi, M. Glia (2003) [Pubmed]
  20. Thrombin stimulates dissociation and induction of HSP27 via p38 MAPK in vascular smooth muscle cells. Hirade, K., Kozawa, O., Tanabe, K., Niwa, M., Matsuno, H., Oiso, Y., Akamatsu, S., Ito, H., Kato, K., Katagiri, Y., Uematsu, T. Am. J. Physiol. Heart Circ. Physiol. (2002) [Pubmed]
  21. S-thiolation of HSP27 regulates its multimeric aggregate size independently of phosphorylation. Eaton, P., Fuller, W., Shattock, M.J. J. Biol. Chem. (2002) [Pubmed]
  22. Innervation-dependent phosphorylation and accumulation of alphaB-crystallin and Hsp27 as insoluble complexes in disused muscle. Kato, K., Ito, H., Kamei, K., Iwamoto, I., Inaguma, Y. FASEB J. (2002) [Pubmed]
  23. MAP kinase pathways involving hsp27 regulate fibroblast-mediated wound contraction. Hirano, S., Rees, R.S., Gilmont, R.R. J. Surg. Res. (2002) [Pubmed]
  24. Follicle stimulating hormone (FSH) activates the p38 mitogen-activated protein kinase pathway, inducing small heat shock protein phosphorylation and cell rounding in immature rat ovarian granulosa cells. Maizels, E.T., Cottom, J., Jones, J.C., Hunzicker-Dunn, M. Endocrinology (1998) [Pubmed]
  25. Benign focal ischemic preconditioning induces neuronal Hsp70 and prolonged astrogliosis with expression of Hsp27. Currie, R.W., Ellison, J.A., White, R.F., Feuerstein, G.Z., Wang, X., Barone, F.C. Brain Res. (2000) [Pubmed]
  26. Vasopressin phosphorylates HSP27 in aortic smooth muscle cells. Akamatsu, S., Nakajima, K., Ishisaki, A., Matsuno, H., Tanabe, K., Takei, M., Takenaka, M., Hirade, K., Yoshimi, N., Suga, H., Oiso, Y., Kato, K., Kozawa, O. J. Cell. Biochem. (2004) [Pubmed]
  27. Expression of the activating transcription factor 3 prevents c-Jun N-terminal kinase-induced neuronal death by promoting heat shock protein 27 expression and Akt activation. Nakagomi, S., Suzuki, Y., Namikawa, K., Kiryu-Seo, S., Kiyama, H. J. Neurosci. (2003) [Pubmed]
  28. Cholecystokinin stimulates heat shock protein 27 phosphorylation in rat pancreas both in vivo and in vitro. Groblewski, G.E., Grady, T., Mehta, N., Lambert, H., Logsdon, C.D., Landry, J., Williams, J.A. Gastroenterology (1997) [Pubmed]
  29. Inactivity-induced modulation of Hsp20 and Hsp25 content in rat hindlimb muscles. Huey, K.A., Thresher, J.S., Brophy, C.M., Roy, R.R. Muscle Nerve (2004) [Pubmed]
  30. Variation in expression of hsp27 messenger ribonucleic acid during the cycle of the seminiferous epithelium and co-localization of hsp27 and microfilaments in Sertoli cells of the rat. Welsh, M.J., Wu, W., Parvinen, M., Gilmont, R.R. Biol. Reprod. (1996) [Pubmed]
  31. A role for the p38 mitogen-activated protein kinase/Hsp 27 pathway in cholecystokinin-induced changes in the actin cytoskeleton in rat pancreatic acini. Schäfer, C., Ross, S.E., Bragado, M.J., Groblewski, G.E., Ernst, S.A., Williams, J.A. J. Biol. Chem. (1998) [Pubmed]
  32. Heat shock protein 27 in chronic allograft nephropathy: a local stress response. Djamali, A., Reese, S., Oberley, T., Hullett, D., Becker, B. Transplantation (2005) [Pubmed]
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