Disease relevance of lectin-24Db

• This result suggests that the toxicity of the lectin is correlated to its ability to bind sugar in the insect digestive tract and stresses the importance of sugar composition of the nutriment used for toxicological testing of lectins or in genetically modified plants [1].
• The reporter gene was expressed in the fat bodies of the transgenic larvae when they were immunized by body pricking or treatment with Escherichia coli, which was the same as the Sarcophaga lectin gene expression in Sarcophaga larvae [2].
• D-Galactose-specific lectins, Bauhina purpura agglutinin (BPA) and Arachis hypogea agglutinin (peanut agglutinin, PNA), and a D-galactose/N-acetylgalactosamine-specific lectin, Glycine max agglutinin (soybean agglutinin, SBA), all recognized the surface of most cultured neurons and their axons [3].

High impact information on lectin-24Db

• We report here the development of a mutation-induced marker in mice heterozygous at the Dlb-1 locus which determines the expression of binding sites for the lectin Dolichos biflorus agglutinin (DBA) in intestinal epithelium [4].
• Basement membrane polarizes lectin binding sites of Drosophila larval fat body cells [5].
• Some immune reactive blood cells did not bind lectin [6].
• We developed a novel genetic approach introducing cDNA for a plant lectin, wheat germ agglutinin (WGA), as a transgene under the control of specific promoter elements [7].
• A genetic approach to visualization of multisynaptic neural pathways using plant lectin transgene [7].

Chemical compound and disease context of lectin-24Db

• In toxicological tests with Drosophila melanogaster, we observed a decrease of lectin toxicity when glucose was added to the larva-rearing medium [1].

Biological context of lectin-24Db

• Insect glycobiology: a lectin multigene family in Drosophila melanogaster [8].
• We have identified an endogenous lectin expressed during Drosophila embryogenesis [9].
• Gliolectin is a carbohydrate-binding protein (lectin) that mediates cell adhesion in vitro and is expressed by midline glial cells in the Drosophila melanogaster embryo [10].
• The molecular mass and gene expression patterns of granulocytin were very similar to those of Drosophila lectin that we reported previously (Haq, S., Kubo, T., Kurata, S., Kobayashi, A., and Natori, S. (1996) J. Biol. Chem. 271, 20213-20218) [11].
• The glycosylation of the Drosophila insulin receptor (DIR) has been compared to that of the rat insulin receptor by examining the binding of receptors to the lectins wheat germ agglutinin, Concanavalin-A, and lentil lectin [12].

Anatomical context of lectin-24Db

• The lectin-producing hepatic cells were identified by immunostaining; in the gill, exocrine mucous cells were stained, suggesting that serum fucolectins derive from the liver [13].
• This lectin agglutinated trypsinized and glutaraldehyde-fixed bovine red blood cells in the presence of calcium or magnesium [14].
• Calnexin is a membrane-bound lectin of the endoplasmic reticulum (ER) that binds transiently to newly synthesized glycoproteins [15].
• At low concentrations of the lectin (5-10 mug/ml), cells spread against the glass surface and fused to form syncytia [16].
• Epitopes restricted to proteins from th-forming spermatocytes reacted with lectin Con A (specific for D-Man and/or D-Glc) and antibodies directed against mouse immunoglobulins (AIA) [17].

Associations of lectin-24Db with chemical compounds

• Competition binding studies using monosaccharides demonstrate that CG2958 interacts specifically with fucose and mannose [18].
• The hapten sugar of this lectin was galactose [14].
• The protein product of the CHI3L1 gene, human cartilage 39-kDa glycoprotein (HC-gp39), is a tissue-restricted, chitin-binding lectin and member of glycosyl hydrolase family 18 [19].
• The protein was solubilized with 1% Triton X-100 and 0.5 M sodium chloride and then purified using an alpha-cobratoxin column followed by a lentil lectin affinity column [20].
• The coalescence of cells and morphologic disotrtion induced by wheat germ agglutinin were not inhibited by N-acetylglucosamine, the hapten inhibitor of the lectin, under the conditions utilized in this study [16].

Physical interactions of lectin-24Db

• The amino acid composition and lectin-binding properties of Drosophila laminin are given [21].

Other interactions of lectin-24Db

• The cloned lectin, designated 'gliolectin', possesses a novel protein sequence with a calculated molecular mass of 24,993 [9].
• Helix pomatia lectin, an inducer of Drosophila immune response, binds to hemomucin, a novel surface mucin [22].
• Some of the family members contain other modules for protein-protein interactions, including disulphide-stabilized structures (LDL(r)A, SRCR, frizzled, kringle, Sushi, Wonton and Pan/apple), carbohydrate-recognition domains (C-type lectin and chitin-binding), and other modules (such as zinc finger, CUB, coiled coil and Sina) [23].

Analytical, diagnostic and therapeutic context of lectin-24Db

• Only one of the proteins induced by ecdysone in EH34A3 cells was detected in the NP-40 soluble fraction of radioiodinated cell lysates, even after fractionation by lectin affinity chromatography and immunoprecipitation to enrich for putative ecdysone induced proteins [24].
• Molecular cloning and characterization of a C-type lectin from Ancylostoma ceylanicum: Evidence for a role in hookworm reproductive physiology [25].
• Using reverse transcription PCR, a cDNA corresponding to a putative C-type lectin has been amplified from the hookworm parasite Ancylostoma ceylanicum [25].
• Sequence alignments and sugar specificity showed that this protein is the third member of a new saline-soluble lectin family present in fungi [26].

References