Disease relevance of ZNF395

• Identification of human autologous cytotoxic T-lymphocyte-defined osteosarcoma gene that encodes a transcriptional regulator, papillomavirus binding factor [1].
• Reverse transcription-PCR analysis revealed that PBF was expressed in 16 of 19 cases of bone and soft-tissue sarcoma cell lines (5 of 6 of osteosarcoma lines) and 57 of 76 sarcoma tissue samples (11 of 14 of osteosarcoma tissues) [1].
• We next analyzed the relationship between PBF/BW and the data from hepatic function tests (serum albumin, total bilirubin, indocyanine green 15-min retention rate, and indocyanine green plasma disappearance rate) in 48 patients with liver cirrhosis [2].
• We investigated the distribution of portal blood flow per kilogram of body weight (PBF/BW) in 112 healthy volunteers and 90 patients with liver cirrhosis using an ultrasonic Doppler duplex system [2].
• The PBF/BW in healthy volunteers showed a log-normal distribution, while the distribution was irregular and showed two peaks in patients with cirrhosis [2].

Psychiatry related information on ZNF395

• Novel nuclear shuttle proteins, HDBP1 and HDBP2, bind to neuronal cell-specific cis-regulatory element in the promoter for the human Huntington's disease gene [3].

High impact information on ZNF395

• The isolated cDNA was identical to papillomavirus binding factor (PBF), which was recently reported as a DNA binding transcription factor cooperating with RUNX1 [1].
• Also, PBF was expressed in 10 of 13 epithelial cancer cell lines and 20 of 34 of cancer tissues [1].
• In contrast, PBF was detected in some normal organs including ovary, pancreas, spleen, and liver by reverse transcription-PCR but was restricted in the cytoplasm by immunostaining and undetectable by Western blotting [1].
• After an intensive work-up, 49 patients with recurrent (REC) and/or metastatic (MTS) UCNT were treated with three monthly cycles of cisplatin (CDDP) 100 mg/m2 day 1; bleomycin 15 mg intravenously (IV) day 1, and 16 mg/m2/d continuous infusion (CI) days 1 to 5; and fluorouracil (5FU) 650 mg/m2/d CI days 1 to 5 (PBF) [4].
• In human cultured cells, ectopically expressed green fluorescent protein-fused HDBP1 and HDBP2 localized in the cytoplasm, but both proteins totally shift from cytoplasm to nucleus by the treatment with an inhibitor of the nuclear export, leptomycin B, and mutagenesis of the putative nuclear export signals [3].

Chemical compound and disease context of ZNF395

• From March 1986 to February 1991, 64 patients with advanced untreated but resectable head and neck cancer who would require total laryngectomy were enrolled on one of two cisplatin-based induction regimens: cisplatin-bleomycin-5-fluorouracil (PBF) in 31 patients and cisplatin-5-fluorouracil (PF) in 33; all received definitive radiotherapy [5].
• Protein-Bound Fucose (PBF) content in sera from normal persons and untreated lepromatous or near lepromatous leprosy patients was measured and statistically analysed [6].

Biological context of ZNF395

• Taken together, HDBP1 and HDBP2 are novel transcription factors shuttling between nucleus and cytoplasm and bind to the specific GCCGGCG, which is an essential cis-element for HD gene expression in neuronal cells [3].
• In this study, we identified two proteins, HDBP1 and HDBP2, which bind to the promoter region for the HD gene using a yeast one-hybrid system [3].
• By a yeast one-hybrid system we could identify an unknown protein binding also to P2, tentatively called PBF (papillomavirus binding factor) [7].
• Point mutations destroying PBF binding to HPV8 P2 and BPV-1 E2 BS-1 in vitro reduce promoter activity in corresponding reporter constructs [7].
• Respiratory rate, tidal volume, dynamic lung compliance, functional residual capacity, and pulmonary resistance were measured withim 24 hours of cardiac catheterization in 25 infants, 12 of whom had increased pulmonary blood flow and 13 of whom had decreased PBF [8].

Anatomical context of ZNF395

• The decrease in CL in infants with increased PBF significantly correlated with mean pulmonary artery pressure (r = 0.798) [8].
• RESULTS: E1-FA levels strongly correlated with BMI (r = 0.69, P = 0.001 in men; r = 0.75, P < 0.0001, in women) percent body fat (PBF, r = 0.52. P = 0.018 in men; and r = 0.69, P < 0.0001, in women) and with the sum of 4 fat skinfolds (sigma skinfolds) [9].
• CONCLUSIONS: There was no evidence that breast milk was replaced by water, tea or juice in PBF compared to EBF infants [10].
• CI was correlated with PI (P<0.001), PW (P<0.001), PA (P<0.001), RA (P=0.043), RVED (P=0.005), HI (P<0.001), AO (P<0.001), CO (P<0.001), LVED (P<0.001), Vmax (P<0.001), Vmean (P<0.001), cross-sectional area of the main portal vein (P<0.001) and PBF (P<0.001) [11].
• Of the "five T's" of cyanotic congenital heart disease--tetralogy of Fallot, TGA, TAPVC, truncus, and tricuspid valve abnormalities (tricuspid atresia, stenosis, and displacement)--the first and last are commonly associated with diminished PBF [12].

Associations of ZNF395 with chemical compounds

• The mechanism for direct insertion of O(2) in a toluene-solvated palladium-hydride bond (avoiding palladium zero) has been elucidated using quantum mechanics (B3LYP/LACVP** with the PBF polarizable continuum solvent model) for Pd(II)(-)-sparteine)(Cl)(H) and the model compound Pd(II)(bipyridine)(Cl)(H) [13].
• The key interactions responsible for the unique reactivity of (-)-sparteine-PdX(2) complexes (X = chloride, acetate) in the enantioselective oxidation of secondary alcohols have been elucidated using quantum mechanics (B3LYP DFT with the PBF polarizable continuum solvent model) [14].
• Protein-DNA interaction studies indicate that three thymidine residues located at the 3'-end of the palindromic sequence motif are important for maximal PBF-binding activity [15].

Physical interactions of ZNF395

• These peptide motifs are not only conserved among the TCF family members but are also found in two newly identified DNA-binding proteins named papillomavirus binding factor and GLUT4 enhancer factor [16].

Other interactions of ZNF395

• We report that NR4A1 interacts with human papillomavirus type 16 (HPV16) E2 protein - a key papillomavirus regulatory factor [17].

Analytical, diagnostic and therapeutic context of ZNF395

• These findings suggest that PBF is a shared tumor-associated antigen, which may serve as a source of peptides applicable to peptide-based immunotherapy for osteosarcoma and other malignant tumors [1].
• METHODS: Body composition methods, including skinfolds, bioelectric impedance analysis (BIA), body mass index (BMI), and using a combination of body composition methods were selected to assess 3-year changes in PBF in 705 children within 21 intervention schools and 663 children within 20 control schools [18].
• Five variables were selected as factors affecting postoperative TBPC; bleeding volume (BLD), warm ischemic time (WIT), cold ischemic time (CIT), graft weight/standard liver volume ratio (GW/SLV), and portal blood flow after surgery (PBF) [19].
• Peripheral BF was measured (laser Doppler technique) in the palmar region (PBF) and the manubrium sterni (SBF), together with ECG, respiration activity (RA) and blood pressure (BP), at rest (5 minute) and during 70 degrees head-up tilt test [20].

References