Disease relevance of Trenbolone

• The potential role of satellite cells in mediating the effect of trenbolone [17 beta-hydroxyestra-4,9-11-trien-3-one (TBOH)] on skeletal muscle hypertrophy was examined [1].
• These data showed that late-gestation treatment with 600 mg of trenbolone acetate significantly increased weight gain of dams without demonstrating any androgenizing effects on the growth or physical characteristics of heifer calves [2].
• Body weight and tissue gain in lambs fed an all-concentrate diet and implanted with trenbolone acetate or grazed on alfalfa [3].
• However, little is known concerning the toxicity of trenbolone to fish [4].
• Fifty crossbred cows (38 multiparous and 12 nulliparous) were used to evaluate in utero androgenization of heifer calves with trenbolone acetate [2].

High impact information on Trenbolone

• Additionally both 1 and 10 nM trenbolone increased IGF-I mRNA levels to 1.7-times control values (P < 0.02) [5].
• Both E(2) and trenbolone increased (3)H-thymidine incorporation rate (1.5-times control levels, P < 0.001) in BSC cultures in media containing serum from which IGFBP-3 had been removed by anti-IGFBP-3 affinity chromatography [5].
• Androgen receptor mRNA levels also were increased (1.5-times control levels, P < 0.02) in cultures treated with 0.001 nM trenbolone but not by treatment with higher concentrations of trenbolone [5].
• Study of various transforming effects of the anabolic agents trenbolone and testosterone on Syrian hamster embryo cells [6].
• Trenbolone alters the responsiveness of skeletal muscle satellite cells to fibroblast growth factor and insulin-like growth factor I [1].

Chemical compound and disease context of Trenbolone

• Eighty steers with a mean body weight of 319 kg were used in a study to evaluate the effect of a growth-promoting implant (trenbolone acetate plus estradiol benzoate), monensin, and oxytetracycline on the steer performance and shedding of some foodborne pathogens [7].
• In a longitudinal study (165 days), we investigated the effect of growth-promoting agents (monensin and trenbolone acetate-estradiol) and an antibiotic (oxytetracycline) on the incidence in feedlot steers of Escherichia coli O157, including antibiotic-resistant and hypermutable isolates [8].

Biological context of Trenbolone

• Allyl trenbolone (Regumate) was used to synchronize estrus in 13 postpuberal gilts [9].
• Assay sensitivities for calibration curves in buffer solutions around 0.5 ng ml(-1) in terms of IC50 (concentration of the inhibitor) values were achieved for the beta-agonist clenbuterol and the hormone analogues ethinylestradiol and trenbolone [10].
• Three 200-mg trenbolone acetate (Finaplix-H) implants were implanted in the ear of treated cows (n = 24) on d 214 +/- 11 of gestation; the remaining animals (n = 26) were used as controls [2].
• Two trials were conducted to evaluate the effectiveness of a synthetic progestogen, allyl trenbolone, for control of estrus and ovulation in gilts [11].
• It is concluded that trenbolone acetate affected the ovarian cycle of the cows in several ways through changes other than the modification of tonic luteinising hormone secretion [12].

Anatomical context of Trenbolone

• No changes were detectable at codons 12, 13, 59, 61, and 117 or adjacent regions in tumor cell lines induced by diethylstilbestrol, asbestos, benzo(a)pyrene, trenbolone, or aflatoxin B1 [13].
• A one-time injection of trenbolone into yolks was administered to mimic maternal deposition, and subsequent effects on the development and function of the immune system were determined in chicks and adults [14].
• Effects of zeranol and trenbolone acetate on testis function, live weight gain and carcass traits of bulls [15].
• Trenbolone acetate had no effect on subcutaneous fat measurements in either rams or ewes [16].
• Morphological transformation of Syrian hamster embryo fibroblasts by the anabolic agent trenbolone [17].

Associations of Trenbolone with other chemical compounds

• Trenbolone acetate is a synthetic anabolic steroid that is widely used in the United States to promote growth of beef cattle [18].
• Metabolites of trenbolone acetate include the stereoisomers 17alpha- and 17beta-trenbolone, both of which are stable in animal wastes and are relatively potent androgens in fish and mammals [18].
• Proceedings: The determination by radioimmunoassay of residues of an anabolic steroid in tissues of calves treated with a combined preparation of trenbolone acetate and oestradiol-17 beta [19].
• Mixture experiments with flutamide and the androgen 17beta-trenbolone demonstrated that the anti-androgen effectively blocked trenbolone-induced masculinization (nuptial tubercle production) of female fathead minnows, indicating antagonism of an AR receptor-mediated response in vivo [20].
• On days 0, 2, 4, 7 and 10, zeranol and nandrolone were quantified by selective ion monitoring analysis on a gas chromatography-mass selective detector and trenbolone by using an enzyme linked immunosorbent assay [21].

Gene context of Trenbolone

• Stimulation of circulating insulin-like growth factor I (IGF-I) and insulin-like growth factor binding proteins (IGFBP) due to administration of a combined trenbolone acetate and estradiol implant in feedlot cattle [22].
• Allyl trenbolone abolished pulsatile LH secretion, and realimentation did not stimulate LH or FSH secretion, with the exception of FSH secretion on Day 8 in A9 gilts [23].
• Female sheep were implanted with the anabolic agent trenbolone acetate and the effects of the treatment on the skeletal muscle androgen receptor studied [24].
• Effects of restricted feeding, low-energy diet, and implantation of trenbolone acetate plus estradiol on growth, carcass traits, and circulating concentrations of insulin-like growth factor (IGF)-I and IGF-binding protein-3 in finishing barrows [25].
• When estradiol (20, 200, and 500 mg) and trenbolone acetate (140, 1400, 3500 mg) alone and in combination were implanted in female calves, blood glucose, GOT, GPT, alkaline phosphatase, LDH, cholesterine and bilirubine; Hb, PVC, quick value; urine density and pH were not affected by treatment [26].

Analytical, diagnostic and therapeutic context of Trenbolone

• All gilts received oral progestogen (15 mg allyl trenbolone) from Day 1 to ovariectomy, to antagonize the usual increases in endogenous gonadotropins that follow realimentation [23].
• Detection of trenbolone residues in meat and organs of slaughtered animals by thin-layer chromatography [27].
• A sensitive and selective method using liquid chromatography (LC)-electrospray mass spectrometry for the determination of growth promoters, trenbolone and zeranol, in bovine muscle and liver has been developed [28].
• Estrus synchronisation and fertility in gilts using a synthetic progestagen (Allyl Trenbolone) and inseminated with fresh stored or frozen semen [29].
• Development of a sensitive microtitration plate enzyme-immunoassay for the anabolic steroid trenbolone [30].

References