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Gene Review

Ivns1abp  -  influenza virus NS1A binding protein

Mus musculus

Synonyms: 1190004M08Rik, 1700126I16Rik, AA960440, HSPC068, Influenza virus NS1A-binding protein homolog, ...
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Disease relevance of Ivns1abp


High impact information on Ivns1abp

  • Here we describe a novel 6:10 chromosomal translocation in the murine plasmacytoma cell line NS-1 which juxtaposes the immunoglobulin Ck gene with a single-copy sequence of unknown function [1].
  • The NS-1 plasmacytoma is a frequently used fusion partner in hybridoma production and is known to contain a rearranged c-myc gene and a genetic element which transforms normal mouse fibroblasts in DNA-mediated transfection assays [1].
  • Using lymphocytes from regional lymph nodes, peripheral blood and tumor infiltrates, 158 fusions were performed with SKO-007 (human myeloma line), LICR-LON-HMy2 (LICR-2), GM 4672 (human lymphoblastoid lines), or NS-1 (mouse myeloma line) [6].
  • This antibody (anti-PAA) was produced by the fusion of myeloma cells NS-1 with spleen cells from a BALB/c mouse, which were sensitized with activated human T-cells generated by allogeneic stimulation in mixed-lymphocyte culture (MLC) [7].
  • However, #71295 was not found in NS-1, D14, and BALB/c normal thymus, liver, colon, and kidney tissues [8].

Chemical compound and disease context of Ivns1abp


Biological context of Ivns1abp

  • Overexpression of Nd1-L in NIH3T3 cells delayed cell growth by affecting the transition of cytokinesis [14].
  • These results suggest that Nd1-S may play a role in cell cycle progression in the nucleus [15].
  • We characterized the genomic organization of the Nd1 gene, and found that Nd1-L and Nd1-S consist of 16 and nine exons respectively, and that exons I-VIII are shared between them [16].
  • Spleen cells of (BAB/ 25 X AKR/J) F1 mice, immunized with dansyl-keyhole limpet hemocyanin (DNS-KLH), were hybridized with NS-1 to generate hybridomas producing monoclonal anti-DNS antibodies of either the b or d haplotype of the BAB/25 or AKR/J parent, respectively [17].
  • The KUN viral cDNA encoded in the plasmid contained the mutation in the NS1 protein (Pro-250 to Leu) previously shown to attenuate KUN virus in weanling mice [18].

Anatomical context of Ivns1abp

  • Nd1-L colocalizes with actin filaments detected using a confocal microscope, and its kelch repeats bind to them in vitro [14].
  • These data suggest that Nd1-L functions as a stabilizer of actin filaments as an actin-binding protein and may play a role in the dynamic organization of the actin cytoskeleton [14].
  • PEMM stimulated with poly I . poly C destroyed allogeneic tumor cells (NS-1) when separated by cell-impermeable Millipore filters in vitro; in contrast, PEMM not stimulated with poly I . poly C were incapable of lysing targets when separated by membranes [19].
  • Thioglycollate-elicited C57BL/6 peritoneal exudate macrophage monolayers (PEMM) stimulated with poly I . poly C or LPS released a macrophage cytotoxin (MCT) that rapidly bound to syngeneic (EL 4) or allogeneic (NS-1, YAC-1) tumor cells but did not bind to normal splenocytes [19].
  • A monoclonal antibody designated anti-Cl was obtained from a hybridoma clone isolated from a fusion of NS1 myeloma with spleen cells from BALB/c mice injected with homogenate of white matter from bovine corpus callosum [20].

Associations of Ivns1abp with chemical compounds

  • Spleen cells of C57BL/6 mice immunized with a highly tumorigenic, chemically induced murine cultured colon 36 tumor (C-C36) of BALB/c origin were hybridized with NS-1, a hypoxanthine phosphoribosyltransferase-deficient myeloma line of BALB/c mice [8].
  • A preliminary probe of the benzyl derivative of vitalethine in a myeloma model (NS-1) suggests efficacy (100% survival) as well [21].
  • In one series of experiments, the growth response of NS-1 cells to several of the intermediates of cholesterol biosynthesis was studied in serum-free medium [22].
  • The major labelled sterol product (greater than 80%) in NS-1 cells after a 24-h exposure to [2-14C]acetate was lanosterol [22].
  • These mutations ablated (i) the TBEV pre-M cleavage site, (ii) the TBEV E glycosylation site, or (iii) the first DEN4 NS1 glycosylation site [23].

Analytical, diagnostic and therapeutic context of Ivns1abp

  • Western blot analysis showed that a monoclonal antibody against recombinant mouse CD14 (mCD14), designated rmC5-3, specifically reacted with mouse macrophage cell line J774, but not myeloma cell line NS1 [24].
  • Southern blot analysis of PstI-digested DNA indicated that in contrast to the parental NIH/3T3 transformed cell lines (pT, T12N/5a, NS-1) all the nude-passaged immunogenic variants (pT-nude, T12N/5a-nude, NS-1-nude) contained newly acquired ecotropic-related proviruses [25].
  • Immunoprecipitation and SDS-PAGE analysis identified 20.5- and 18-kDa proteins with mAb H1-229 or H2-439, respectively, in cellular extracts of 125I-surface labeled NS-1 transfected with the corresponding genes [26].
  • By site-directed mutagenesis, we showed that this residue is essential for NS1 helicase activity but not promoter regulation, suggesting a possible modulation of NS1 functions by PKClambda phosphorylation at residue S473 [27].
  • Thus, because of its T-cell immunogenicity and antigenic simplicity, the NS1 delivery system could serve as a priming agent for heterologous prime-boost vaccination regimens [28].


  1. A novel 6:10 chromosomal translocation in the murine plasmacytoma NS-1. Perlmutter, R.M., Klotz, J.L., Pravtcheva, D., Ruddle, F., Hood, L. Nature (1984) [Pubmed]
  2. Inhibition of respiratory syncytial virus infection with intranasal siRNA nanoparticles targeting the viral NS1 gene. Zhang, W., Yang, H., Kong, X., Mohapatra, S., San Juan-Vergara, H., Hellermann, G., Behera, S., Singam, R., Lockey, R.F., Mohapatra, S.S. Nat. Med. (2005) [Pubmed]
  3. Measurement of suppressor transfer RNA activity. Young, J.F., Capecchi, M., Laski, F.A., RajBhandary, U.L., Sharp, P.A., Palese, P. Science (1983) [Pubmed]
  4. Expression of the 6C3 antigen on murine hematopoietic neoplasms. Association with expression of abl, ras, fes, src, erbB, and Cas NS-1 oncogenes but not with myc. Morse, H.C., Tidmarsh, G.F., Holmes, K.L., Frederickson, T.F., Hartley, J.N., Pierce, J.H., Langdon, W.Y., Dailey, M.O., Weissman, I.L. J. Exp. Med. (1987) [Pubmed]
  5. The truncation that generated the v-cbl oncogene reveals an ability for nuclear transport, DNA binding and acute transformation. Blake, T.J., Heath, K.G., Langdon, W.Y. EMBO J. (1993) [Pubmed]
  6. Detection of cell surface and intracellular antigens by human monoclonal antibodies. Hybrid cell lines derived from lymphocytes of patients with malignant melanoma. Houghton, A.N., Brooks, H., Cote, R.J., Taormina, M.C., Oettgen, H.F., Old, L.J. J. Exp. Med. (1983) [Pubmed]
  7. Unique proliferation-associated marker expressed on activated and transformed human cells defined by monoclonal antibody. Dubey, D.P., Staunton, D.E., Parekh, A.C., Schwarting, G.A., Antoniou, D., Lazarus, H., Yunis, E.J. J. Natl. Cancer Inst. (1987) [Pubmed]
  8. Identification of transformation-related antigen by monoclonal antibody on Swiss 3T3 cells induced by transfection with murine cultured colon 36 tumor DNA. Sato, N., Sato, T., Takahashi, S., Okubo, M., Yagihashi, A., Koshiba, H., Kikuchi, K. J. Natl. Cancer Inst. (1987) [Pubmed]
  9. Identification of a human cancer-associated antigen defined with monoclonal antibody. Bhattacharya, M., Chatterjee, S.K., Barlow, J.J. Cancer Res. (1984) [Pubmed]
  10. Monoclonal antibody analysis of lipopolysaccharide from Neisseria gonorrhoeae and Neisseria meningitidis. Apicella, M.A., Bennett, K.M., Hermerath, C.A., Roberts, D.E. Infect. Immun. (1981) [Pubmed]
  11. Growth restriction of dengue virus type 2 by site-specific mutagenesis of virus-encoded glycoproteins. Pryor, M.J., Gualano, R.C., Lin, B., Davidson, A.D., Wright, P.J. J. Gen. Virol. (1998) [Pubmed]
  12. In situ reactions of monoclonal antibodies with a viable mutant of Murray Valley encephalitis virus reveal an absence of dimeric NS1 protein. Clark, D.C., Lobigs, M., Lee, E., Howard, M.J., Clark, K., Blitvich, B.J., Hall, R.A. J. Gen. Virol. (2007) [Pubmed]
  13. Overexpression of Nd1, a novel Kelch family protein, in the heart of transgenic mice protects against doxorubicin-induced cardiomyopathy. Matsudo, Y., Takamori, Y., Fujimura, L., Nishio, S., Sasagawa, K., Komuro, I., Tokuhisa, T., Hatano, M. Transgenic Res. (2006) [Pubmed]
  14. Identification of Nd1, a novel murine kelch family protein, involved in stabilization of actin filaments. Sasagawa, K., Matsudo, Y., Kang, M., Fujimura, L., Iitsuka, Y., Okada, S., Ochiai, T., Tokuhisa, T., Hatano, M. J. Biol. Chem. (2002) [Pubmed]
  15. Overexpression of Nd1-s, a variant form of new kelch family protein, perturbs the cell cycle progression of fibroblasts. Inoue, A., Kang, M., Fujimura, L., Takamori, Y., Sasagawa, K., Itoh, H., Tokuhisa, T., Hatano, M. DNA Cell Biol. (2005) [Pubmed]
  16. Nd1, a novel murine Kelch family protein, may play the role of a housekeeping gene. Kang, M., Matsudo, Y., Sasagawa, K., Tokuhisa, T., Hatano, M. Biochim. Biophys. Acta (2001) [Pubmed]
  17. Homologous chromosome recombination generating immunoglobulin allotype and isotype switch variants. Kipps, T.J., Herzenberg, L.A. EMBO J. (1986) [Pubmed]
  18. DNA vaccine coding for the full-length infectious Kunjin virus RNA protects mice against the New York strain of West Nile virus. Hall, R.A., Nisbet, D.J., Pham, K.B., Pyke, A.T., Smith, G.A., Khromykh, A.A. Proc. Natl. Acad. Sci. U.S.A. (2003) [Pubmed]
  19. Inducible macrophage cytotoxins. II. Tumor lysis mechanism involving target cell-binding proteases. Reidarson, T.H., Granger, G.A., Klostergaard, J. J. Natl. Cancer Inst. (1982) [Pubmed]
  20. Recognition of Bergmann glial and ependymal cells in the mouse nervous system by monoclonal antibody. Sommer, I., Lagenaur, C., Schachner, M. J. Cell Biol. (1981) [Pubmed]
  21. Vitalethine modulates erythropoiesis and neoplasia. Knight, G.D., Laubscher, K.H., Fore, M.L., Clark, D.A., Scallen, T.J. Cancer Res. (1994) [Pubmed]
  22. Biochemical characterization of the cholesterol-dependent growth of the NS-1 mouse myeloma cell line. Chen, J.K., Okamoto, T., Sato, J.D., Sato, G.H., McClure, D.B. Exp. Cell Res. (1986) [Pubmed]
  23. Chimeric tick-borne encephalitis and dengue type 4 viruses: effects of mutations on neurovirulence in mice. Pletnev, A.G., Bray, M., Lai, C.J. J. Virol. (1993) [Pubmed]
  24. Upregulation of mouse CD14 expression in Kupffer cells by lipopolysaccharide. Matsuura, K., Ishida, T., Setoguchi, M., Higuchi, Y., Akizuki, S., Yamamoto, S. J. Exp. Med. (1994) [Pubmed]
  25. Involvement of somatically acquired ecotropic viruses in the immunogenicity of nude-transplanted NIH/3T3 transformed cell lines. Sensi, M., Bergomi, M., Panceri, P., Castelli, C., Lupetti, R., Traversari, C., Carbone, G., Parmiani, G. J. Immunol. (1992) [Pubmed]
  26. Expression of nm23/NDP kinase proteins on the cell surface. Urano, T., Furukawa, K., Shiku, H. Oncogene (1993) [Pubmed]
  27. DNA unwinding functions of minute virus of mice NS1 protein are modulated specifically by the lambda isoform of protein kinase C. Dettwiler, S., Rommelaere, J., Nüesch, J.P. J. Virol. (1999) [Pubmed]
  28. Induction of human immunodeficiency virus type 1-specific T cells by a bluetongue virus tubule-vectored vaccine prime-recombinant modified virus Ankara boost regimen. Larke, N., Murphy, A., Wirblich, C., Teoh, D., Estcourt, M.J., McMichael, A.J., Roy, P., Hanke, T. J. Virol. (2005) [Pubmed]
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