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Gene Review

CYP3A7  -  cytochrome P450, family 3, subfamily A,...

Homo sapiens

Synonyms: CP37, CYPIIIA7, Cytochrome P450 3A7, Cytochrome P450-HFLA, P-450(HFL33), ...
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Disease relevance of CYP3A7


High impact information on CYP3A7

  • CYP3A7, a form found prominently in human fetal liver microsomes, was first isolated as a liver 16-alpha-dehydroepiandrosterone-sulfate hydroxylase [6].
  • Identification of the fetal liver cytochrome CYP3A7 in human endometrium and placenta [6].
  • In addition, during the secretory phase of the menstrual cycle CYP3A7 expression was sixfold higher than in the one sample from the proliferative phase that had detectable CYP3A7 mRNA [6].
  • Moreover, the amounts of placental and endometrial CYP3A7 mRNA and protein increased substantially from the first to the second trimester of pregnancy [6].
  • Amplification of the same RNA samples with the use of primers specific for CYP3A7, produced a 552-bp segment that had the predicted size and the same DNA sequence as does liver CYP3A7 cDNA [6].

Chemical compound and disease context of CYP3A7


Biological context of CYP3A7

  • Thus, during organogenesis, human embryonic hepatic tissues express primarily CYP3A7 and are capable of significant CYP3A7-catalyzed xenobiotic monooxygenation during this very early stage of gestation [8].
  • Sequencing data indicated that plasmids in 58 of 59 recombinant positive colonies contained an insert with a sequence identical to that present in CYP3A7 cDNA and the plasmid of only one colony contained an insert with a sequence identical to that present in CYP3A5 cDNA [8].
  • A novel polymorphic cytochrome P450 formed by splicing of CYP3A7 and the pseudogene CYP3AP1 [9].
  • In conclusion, a novel mechanism, consisting of the splicing of the pseudogene CYP3AP1 to CYP3A7, causes the formation of the novel CYP3A7.1L having a different tissue distribution and functional properties than the parent CYP3A7 enzyme, with possible developmental, physiological, and toxicological consequences [9].
  • Taken together, the results indicated that CYP3A7 was the major if not sole isoform responsible for catalysis of the N-demethylation of imipramine in human hepatic tissues during embryogenesis [10].

Anatomical context of CYP3A7


Associations of CYP3A7 with chemical compounds


Physical interactions of CYP3A7

  • Taken together, the decrease of VDR binding to the proximal ER6 caused by the mutation results in the loss of CYP3A7 gene activation by 1,25(OH)(2)D(3) [18].

Regulatory relationships of CYP3A7


Other interactions of CYP3A7

  • CYP3A7 mRNA was consistently detected only in the liver by PCR and CYP3A3 mRNA was not detected in any of the tissues [20].
  • CYP1A2 and CYP3A7 were not detected in HSC [21].
  • Interestingly, CYP3A7, a gene that is preferentially expressed in the fetal liver, was expressed constitutively neither in confluent nor in subconfluent cultures, irrespective of the presence of EGF [22].
  • The second group contained CYP-IID, CYP3A7 and CYP27A1, genes that showed high levels of expression specific to well differentiated HCC [23].
  • However, CYP3A7 mRNA has recently been shown to be expressed at significant levels in a subset of adult human livers, several of which carry the CYP3A7*1C allele that contains the proximal PXR/CAR element of CYP3A4 [17].

Analytical, diagnostic and therapeutic context of CYP3A7

  • Northern blot analysis of total RNA isolated from placenta or from endometrium demonstrated a single band that cross-hybridized with a CYP3A7 cDNA [6].
  • RT-PCR showed that both CYP3A5 mRNA and CYP3A7 mRNA were consistently present in both tumour and normal samples, while CYP3A4 mRNA was present in 65% of tumours and 90% of normal samples [24].
  • The expression and inducibility of CYP3A7 transgene in the fetus and suckling neonates from one of the transgenic lines (M10) were investigated by Northern and Western blot analyses [25].
  • Contrary to the hypothesis, chromatin immunoprecipitation experiments showed that the genomic fragment harboring the proximal element was preferably precipitated over the fragment containing the distant element in the CYP3A4 gene, but the opposite was true with the CYP3A7 gene [26].
  • In immunoblotting analysis, this antibody did not recognize CYP3A5 or CYP3A7 in microsomes prepared from baculovirus-infected cells containing these two expressed isoforms [27].


  1. Novel transcriptional regulation of the human CYP3A7 gene by Sp1 and Sp3 through nuclear factor kappa B-like element. Saito, T., Takahashi, Y., Hashimoto, H., Kamataki, T. J. Biol. Chem. (2001) [Pubmed]
  2. Development of bacterial expression system with high yield of CYP3A7, a human fetus-specific form of cytochrome P450. Inoue, E., Takahashi, Y., Imai, Y., Kamataki, T. Biochem. Biophys. Res. Commun. (2000) [Pubmed]
  3. Establishment of transgenic mice carrying human fetus-specific CYP3A7. Li, Y., Yokoi, T., Kitamura, R., Sasaki, M., Gunji, M., Katsuki, M., Kamataki, T. Arch. Biochem. Biophys. (1996) [Pubmed]
  4. Cytotoxicity of chloroacetanilide herbicide alachlor in HepG2 cells independent of CYP3A4 and CYP3A7. Miranda, S.R., Meyer, S.A. Food Chem. Toxicol. (2007) [Pubmed]
  5. Efficient complementary DNA directed expression of human fetal liver cytochrome P450 (CYP3A7) in insect cells using baculovirus. Sakuma, T., Kitamura, R., Yokoi, T., Kamataki, T. Biochem. Mol. Biol. Int. (1995) [Pubmed]
  6. Identification of the fetal liver cytochrome CYP3A7 in human endometrium and placenta. Schuetz, J.D., Kauma, S., Guzelian, P.S. J. Clin. Invest. (1993) [Pubmed]
  7. Steroid hydroxylation by human fetal CYP3A7 and human NADPH-cytochrome P450 reductase coexpressed in insect cells using baculovirus. Ohmori, S., Fujiki, N., Nakasa, H., Nakamura, H., Ishii, I., Itahashi, K., Kitada, M. Res. Commun. Mol. Pathol. Pharmacol. (1998) [Pubmed]
  8. Functional cytochrome P4503A isoforms in human embryonic tissues: expression during organogenesis. Yang, H.Y., Lee, Q.P., Rettie, A.E., Juchau, M.R. Mol. Pharmacol. (1994) [Pubmed]
  9. A novel polymorphic cytochrome P450 formed by splicing of CYP3A7 and the pseudogene CYP3AP1. Rodriguez-Antona, C., Axelson, M., Otter, C., Rane, A., Ingelman-Sundberg, M. J. Biol. Chem. (2005) [Pubmed]
  10. Catalysis of drug oxidation during embryogenesis in human hepatic tissues using imipramine as a model substrate. Chen, H., Brzezinski, M.R., Fantel, A.G., Juchau, M.R. Drug Metab. Dispos. (1999) [Pubmed]
  11. Expression of enzymatically active CYP3A4 by Caco-2 cells grown on extracellular matrix-coated permeable supports in the presence of 1alpha,25-dihydroxyvitamin D3. Schmiedlin-Ren, P., Thummel, K.E., Fisher, J.M., Paine, M.F., Lown, K.S., Watkins, P.B. Mol. Pharmacol. (1997) [Pubmed]
  12. Cytochrome P450 (CYP) expression in human myeloblastic and lymphoid cell lines. Nagai, F., Hiyoshi, Y., Sugimachi, K., Tamura, H.O. Biol. Pharm. Bull. (2002) [Pubmed]
  13. Expression of CYP3A4, CYP3A5 and CYP3A7 in human duodenal tissue. Kivistö, K.T., Bookjans, G., Fromm, M.F., Griese, E.U., Münzel, P., Kroemer, H.K. British journal of clinical pharmacology. (1996) [Pubmed]
  14. Characterization of the oxidative metabolites of 17beta-estradiol and estrone formed by 15 selectively expressed human cytochrome p450 isoforms. Lee, A.J., Cai, M.X., Thomas, P.E., Conney, A.H., Zhu, B.T. Endocrinology (2003) [Pubmed]
  15. Catalysis of the 4-hydroxylation of retinoic acids by cyp3a7 in human fetal hepatic tissues. Chen, H., Fantel, A.G., Juchau, M.R. Drug Metab. Dispos. (2000) [Pubmed]
  16. A review of developmental aspects of cytochrome P450. Oesterheld, J.R. Journal of child and adolescent psychopharmacology. (1998) [Pubmed]
  17. CYP3A7 protein expression is high in a fraction of adult human livers and partially associated with the CYP3A7*1C allele. Sim, S.C., Edwards, R.J., Boobis, A.R., Ingelman-Sundberg, M. Pharmacogenet. Genomics (2005) [Pubmed]
  18. Loss of CYP3A7 gene induction by 1,25-dihydroxyvitamin D3 is caused by less binding of VDR to the proximal ER6 in CYP3A7 gene. Hara, H., Yasunami, Y., Adachi, T. Biochem. Biophys. Res. Commun. (2004) [Pubmed]
  19. Differential catalytic properties in metabolism of endogenous and exogenous substrates among CYP3A enzymes expressed in COS-7 cells. Ohmori, S., Nakasa, H., Asanome, K., Kurose, Y., Ishii, I., Hosokawa, M., Kitada, M. Biochim. Biophys. Acta (1998) [Pubmed]
  20. CYP3A gene expression in human gut epithelium. Kolars, J.C., Lown, K.S., Schmiedlin-Ren, P., Ghosh, M., Fang, C., Wrighton, S.A., Merion, R.M., Watkins, P.B. Pharmacogenetics (1994) [Pubmed]
  21. Cytochrome p450 and glutathione s-transferase mRNA expression in human fetal liver hematopoietic stem cells. Shao, J., Stapleton, P.L., Lin, Y.S., Gallagher, E.P. Drug Metab. Dispos. (2007) [Pubmed]
  22. Effect of cell density and epidermal growth factor on the inducible expression of CYP3A and CYP1A genes in human hepatocytes in primary culture. Greuet, J., Pichard, L., Ourlin, J.C., Bonfils, C., Domergue, J., Le Treut, P., Maurel, P. Hepatology (1997) [Pubmed]
  23. Patterns of expression of cytochrome P450 genes in progression of hepatitis C virus-associated hepatocellular carcinoma. Tsunedomi, R., Iizuka, N., Hamamoto, Y., Uchimura, S., Miyamoto, T., Tamesa, T., Okada, T., Takemoto, N., Takashima, M., Sakamoto, K., Hamada, K., Yamada-Okabe, H., Oka, M. Int. J. Oncol. (2005) [Pubmed]
  24. Cytochrome P450 CYP3A in human renal cell cancer. Murray, G.I., McFadyen, M.C., Mitchell, R.T., Cheung, Y.L., Kerr, A.C., Melvin, W.T. Br. J. Cancer (1999) [Pubmed]
  25. Perinatal expression and inducibility of human CYP3A7 in C57BL/6N transgenic mice. Li, Y., Yokoi, T., Sasaki, M., Hattori, K., Katsuki, M., Kamataki, T. Biochem. Biophys. Res. Commun. (1996) [Pubmed]
  26. The pregnane X receptor binds to response elements in a genomic context-dependent manner, and PXR activator rifampicin selectively alters the binding among target genes. Song, X., Xie, M., Zhang, H., Li, Y., Sachdeva, K., Yan, B. Drug Metab. Dispos. (2004) [Pubmed]
  27. Inhibitory anti-CYP3A4 peptide antibody: mapping of inhibitory epitope and specificity toward other CYP3A isoforms. Wang, R.W., Newton, D.J., Liu, N.Y., Shou, M., Rushmore, T., Lu, A.Y. Drug Metab. Dispos. (1999) [Pubmed]
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