The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
Gene Review

OXA-1  -  hypothetical protein

Escherichia coli

Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of OXA-1

  • Positive associations were found between E. coli and TEM-1 or OXA-1 and between K. pneumoniae and SHV-1 [1].
  • The Bush type 2c and 2d enzymes OXA-1, OXA-2, PSE-1, PSE-2, and PSE-4 did not hydrolyze LJC 10,627, nor did the beta-lactamases of Staphylococcus aureus, Moraxella spp., Bacteroides fragilis, and Proteus vulgaris [2].
  • Four types of beta-lactamases consisting of a penicillinase type I (TEM-1), a penicillinase type II (OXA-1), a cephalosporinase of Citrobacter freundii, and a cephalosporinase of Proteus vulgaris were introduced into Escherichia coli MC4100 and its omp mutants, MH1160 (MC4100 ompR1) and MH760 (MC4100 ompR2), by transformation [3].
  • AmpS is predicted to be a mature protein of 27 kDa with a pI value of 7.9 that mostly closely resembles BLAD from Klebsiella pneumoniae (42.2%), and OXA-1 from Escherichia coli (36.6%), beta-lactamases that are encoded by genes carried on multiresistant transposons [4].
  • None of the probes hybridized with genes for any of eight oxacillin-hydrolysing enzymes, PSE-2, OXA-1 to OXA-7, ROB-1 and chromosomal beta-lactamases of various Enterobacteriaceae (except Klebsiella pneumoniae) and Pseudomonas aeruginosa [5].

High impact information on OXA-1


Chemical compound and disease context of OXA-1


Biological context of OXA-1

  • AsbB1 was a class D enzyme most closely related to the oxacillin-hydrolyzing enzyme OXA-1, with 34% amino acid sequence identity [11].
  • OXA-47 had a narrow spectrum of hydrolysis activity and did not hydrolyze ceftazidime or imipenem, as is found for the beta-lactamase (OXA-1) to which it is related [12].
  • All but 1 of the 33 E. coli phenotypes found to be TEM-1 positive were uniformly positive for the beta-lactamase gene, whereas some of the phenotypes found to be positive for OXA-1 (2 of 3) and SHV-1 (6 of 70) were occasionally negative for the respective genes [7].
  • In contrast to the structures of OXA-2, OXA-10, and OXA-13 belonging to other subclasses, the OXA-1 molecule is monomeric rather than dimeric and represents the subclass characterized by an enlarged Omega loop near the beta-lactam binding site [13].
  • The combined data indicate that YidC is targeted by the signal recognition particle and inserts at the SecAYEG-YidC translocon early during biogenesis, unlike its mitochondrial homologue Oxa1p [14].

Anatomical context of OXA-1

  • However, to integrate mitochondrial translation products into the inner membrane of mitochondria, the ribosome-binding domain of Oxa1 has to be appended onto YidC [15].
  • We, furthermore, find no evidence for the involvement of known membrane-bound translocation apparatus; proteolysis of thylakoids destroys the Sec and Tat translocons but does not block PsaK insertion, and antibodies against the Oxa1/YidC homolog, Alb3, block the SRP-dependent insertion of Lhcb1 but again have no effect on PsaK insertion [16].

Associations of OXA-1 with chemical compounds

  • We propose the designation Tn1404 for this unit, which, like transposons carrying OXA-1, PSE-1, PSE-4, and some transposons determining TEM-1, includes genes for beta-lactam, aminoglycoside, and sulfonamide resistance [17].
  • PSE-2 beta-lactamase resembled other PSE enzymes in activity against carbenicillin, but it also resembled OXA enzymes, such as OXA-1, in rapid hydrolysis of oxacillin, cloxacillin, and methicillin and in inhibition by sodium chloride but not by cloxacillin [17].
  • Bacteria possessing TEM-1-like beta-lactamases are generally regarded as susceptible to ampicillin-sulbactam (SAM), while those harboring OXA-1 enzymes are considered resistant [18].
  • Ceftibuten was found to be very stable in the presence of five commonly occurring beta-lactamases of both the chromosomal-mediated (P99, K1) and plasmid-mediated (CARB-2, OXA-1, TEM-1) types [19].
  • Cefpodoxime and the other methoxyimino cephalosporins exhibited a poor affinity to the plasmid-mediated TEM-2 and OXA-1 enzymes [20].

Other interactions of OXA-1

  • Isolates producing either AmpC or OXA-1 enzymes or producing high levels of TEM-1 beta-lactamases had susceptibility patterns that were difficult to distinguish without IEF and/or amplification of the corresponding specific genes [21].

Analytical, diagnostic and therapeutic context of OXA-1

  • Crystallization and preliminary X-ray study of OXA-1, a class D beta-lactamase [8].
  • Molecular cloning of DNA fragments between 1.5 and 8 kb from BamHI, EcoRI, HindIII, SalI, or Sau3A digests permitted the isolation of structural genes coding for TEM-1, ROB-1, OXA-1, OXA-3, OXA-4, OXA-5, PSE-1, PSE-2, PSE-3, PSE-4, CARB-3, CARB-4, AER-1, and LCR-1 beta-lactamases [22].
  • The production of TEM 1, OXA 2, CARB 3 and PSE 1 beta-lactamases had no influence on susceptibility to the antibiotic, whereas the synthesis of TEM 2, SHV 1 and OXA 1 beta-lactamases increased minimum inhibitory concentrations (MIC) by 2-4 times [23].
  • Furthermore, the antibody induced change in enzymic activity of various beta-lactamases was demonstrated by activity titration curves for TEM 1, TEM 2, OXA 1, OXA 2, OXA 3 beta-lactamases from E. coli [24].


  1. Principal beta-lactamases responsible for resistance to beta-lactam antibiotics in urinary tract infections. Simpson, I.N., Harper, P.B., O'Callaghan, C.H. Antimicrob. Agents Chemother. (1980) [Pubmed]
  2. In vitro activity and beta-lactamase stability of LJC 10,627. Neu, H.C., Gu, J.W., Fang, W., Chin, N.X. Antimicrob. Agents Chemother. (1992) [Pubmed]
  3. Effects of beta-lactamases and omp mutation on susceptibility to beta-lactam antibiotics in Escherichia coli. Hiraoka, M., Okamoto, R., Inoue, M., Mitsuhashi, S. Antimicrob. Agents Chemother. (1989) [Pubmed]
  4. Sequence analysis of two chromosomally mediated inducible beta-lactamases from Aeromonas sobria, strain 163a, one a class D penicillinase, the other an AmpC cephalosporinase. Walsh, T.R., Hall, L., MacGowan, A.P., Bennett, P.M. J. Antimicrob. Chemother. (1995) [Pubmed]
  5. Construction by polymerase chain reaction and use of intragenic DNA probes for three main types of transferable beta-lactamases (TEM, SHV, CARB) [corrected]. Arlet, G., Philippon, A. FEMS Microbiol. Lett. (1991) [Pubmed]
  6. Precise insertion of antibiotic resistance determinants into Tn21-like transposons: nucleotide sequence of the OXA-1 beta-lactamase gene. Ouellette, M., Bissonnette, L., Roy, P.H. Proc. Natl. Acad. Sci. U.S.A. (1987) [Pubmed]
  7. Epidemiology of plasmid-mediated beta-lactamases in enterobacteria Swedish neonatal wards and relation to antimicrobial therapy. Burman, L.G., Haeggman, S., Kuistila, M., Tullus, K., Huovinen, P. Antimicrob. Agents Chemother. (1992) [Pubmed]
  8. Crystallization and preliminary X-ray study of OXA-1, a class D beta-lactamase. Sun, T., Nukaga, M., Mayama, K., Crichlow, G.V., Kuzin, A.P., Knox, J.R. Acta Crystallogr. D Biol. Crystallogr. (2001) [Pubmed]
  9. Evaluation of plasmid-encoded beta-lactamase resistance in Escherichia coli blood culture isolates. Huovinen, S., Huovinen, P., Torniainen, K., Jacoby, G.A. Eur. J. Clin. Microbiol. Infect. Dis. (1988) [Pubmed]
  10. PBP binding and periplasmic concentration as determinants of the antibacterial activities of three new oral cephalosporins in Escherichia coli. Cornaglia, G., Ligozzi, M., Bauernfeind, A., Satta, G., Fontana, R. New Microbiol. (1994) [Pubmed]
  11. Cloning and expression of a cloxacillin-hydrolyzing enzyme and a cephalosporinase from Aeromonas sobria AER 14M in Escherichia coli: requirement for an E. coli chromosomal mutation for efficient expression of the class D enzyme. Rasmussen, B.A., Keeney, D., Yang, Y., Bush, K. Antimicrob. Agents Chemother. (1994) [Pubmed]
  12. Emergence of oxacillinase-mediated resistance to imipenem in Klebsiella pneumoniae. Poirel, L., Héritier, C., Tolün, V., Nordmann, P. Antimicrob. Agents Chemother. (2004) [Pubmed]
  13. Comparison of beta-lactamases of classes A and D: 1.5-A crystallographic structure of the class D OXA-1 oxacillinase. Sun, T., Nukaga, M., Mayama, K., Braswell, E.H., Knox, J.R. Protein Sci. (2003) [Pubmed]
  14. Targeting, insertion, and localization of Escherichia coli YidC. Urbanus, M.L., Fröderberg, L., Drew, D., Björk, P., de Gier, J.W., Brunner, J., Oudega, B., Luirink, J. J. Biol. Chem. (2002) [Pubmed]
  15. Evolution of mitochondrial oxa proteins from bacterial YidC. Inherited and acquired functions of a conserved protein insertion machinery. Preuss, M., Ott, M., Funes, S., Luirink, J., Herrmann, J.M. J. Biol. Chem. (2005) [Pubmed]
  16. Insertion of PsaK into the thylakoid membrane in a "Horseshoe" conformation occurs in the absence of signal recognition particle, nucleoside triphosphates, or functional albino3. Mant, A., Woolhead, C.A., Moore, M., Henry, R., Robinson, C. J. Biol. Chem. (2001) [Pubmed]
  17. Properties of PSE-2 beta-lactamase and genetic basis for its production in Pseudomonas aeruginosa. Philippon, A.M., Paul, G.C., Jacoby, G.A. Antimicrob. Agents Chemother. (1983) [Pubmed]
  18. An evaluation of susceptibility testing methods for ampicillin-sulbactam using a panel of beta-lactamase-producing bacteria. Siu, L.K., Lo, J.Y., Cheng, W.L., Ho, P.L., Ng, W.S., Chau, P.Y. APMIS (1999) [Pubmed]
  19. Ceftibuten (7432-S, SCH 39720): comparative antimicrobial activity against 4735 clinical isolates, beta-lactamase stability and broth microdilution quality control guidelines. Jones, R.N., Barry, A.L. Eur. J. Clin. Microbiol. Infect. Dis. (1988) [Pubmed]
  20. Cefpodoxime: comparable evaluation with other orally available cephalosporins. With a note on the role of beta-lactamases. Cullmann, W., Dick, W. Zentralbl. Bakteriol. (1990) [Pubmed]
  21. Patterns and mechanisms of resistance to beta-lactams and beta-lactamase inhibitors in uropathogenic Escherichia coli isolated from dogs in Portugal. Féria, C., Ferreira, E., Correia, J.D., Gonçalves, J., Caniça, M. J. Antimicrob. Chemother. (2002) [Pubmed]
  22. Molecular cloning and DNA homology of plasmid-mediated beta-lactamase genes. Levesque, R.C., Medeiros, A.A., Jacoby, G.A. Mol. Gen. Genet. (1987) [Pubmed]
  23. In vitro activity, beta-lactamase stability and PBP affinity of RU 51,746-2, the active metabolite of the new orally absorbed cephalosporin ester, RU 51807. Boaretti, M., Lleó, M.M., Canepari, P. Journal of chemotherapy (Florence, Italy) (1991) [Pubmed]
  24. Mode of interaction between immunoglobulin G and mezlocillin against beta-lactamase producing bacteria. Dalhoff, A., Brunner, H. Arzneimittel-Forschung. (1983) [Pubmed]
WikiGenes - Universities