Disease relevance of IL2

• ISF inhibited proliferation of Ag 8 and X 63-IL-2 B lymphoma cells as well [1].
• Comparison of feline leukemia virus-infected and normal cat T-cell lines in interleukin 2-conditioned medium [2].
• Recombinant interleukin 2 as an adjuvant for vaccine-induced protection. Immunization of guinea pigs with herpes simplex virus subunit vaccines [3].
• Dietary protein deficiency and Mycobacterium bovis BCG affect interleukin-2 activity in experimental pulmonary tuberculosis [4].
• Significant increases in interleukin-2 levels, produced by incubation of mononuclear cells with HSV-2 antigens, but not in circulating antibody also were detected in the treated groups [5].

Psychiatry related information on IL2

• Also, large individual differences in the magnitudes of Con A-induced proliferation and IL-2 production were seen for PBMC collected from individual pigs within each litter [6].

High impact information on IL2

• We also report here that low concentrations of Pro-boroPro inhibit antigen-induced proliferation and interleukin 2 production in murine T-cell lines but do not inhibit the response of these T cells to the mitogen concanavalin A [7].
• Following mitogen stimulation (phytohemagglutinin or concanavalin A), feline lymphocytes were maintained in medium containing feline interleukin 2 [2].
• In contrast, the primary T cell proliferative responses to self-Ia antigens in the syngeneic mixed leukocyte reaction (SMLR) and to trinitrophenyl-modified syngeneic macrophages were blocked by CY A and this suppressive effect could not be corrected by addition of exogenous IL 2 [8].
• However, the cellular infiltrate in the acceptance reaction was distinguished by less T-cell activation (interleukin-2 receptor+), less proliferation (proliferating cell nuclear antigen+) of infiltrating cells, and less graft cell apoptosis in arteries, tubules, glomeruli, and peritubular capillaries [9].
• Previous studies have suggested that this phenomenon is related to limited class I-specific helper T cell activity as evidenced by the failure of antibody class switching in vivo and the ability of exogenous interleukin 2 to elicit antidonor responses in vitro [10].

Chemical compound and disease context of IL2

• Locoregional therapy with polyethylene-glycol-modified interleukin-2 of an intradermally growing hepatocellular carcinoma in the guinea pig induces T-cell-mediated antitumor activity [11].
• These data suggest that pseudorabies virus infection decreased lymphocyte proliferative responses and interleukin-2 production in pigs, and that isoprinosine did not mitigate these effects [12].
• Mitogen-stimulated lymphocyte proliferation, delayed-type hypersensitivity (DTH) reactions, interleukin-2 (IL-2) production, and growth performance were evaluated in 3-week-old pigs treated with imuthiol [13].

Biological context of IL2

• The resulting porcine DNA sequence encodes a 154 residue IL-2 primary translation product [14].
• Comparison of the mature, secreted form of porcine IL-2 with those of other species was carried out in an attempt to identify differences that might contribute to the observed differing species specificities [14].
• Depleted PBMC produced IL-2 which accumulated in cultures instead of being rapidly consumed by IL-2 dependent cell growth [15].
• Dietary protein deficiency and BCG vaccination appear to modulate antigen-driven cellular immunity in animals with tuberculosis by altering the production of, and the response to, IL-2 by PPD-stimulated lymphocytes [4].
• This clone could mount an IL-2-dependent, tissue-specific, non-major histocompatibility complex (MHC)-restricted proliferative response to OD from rat, guinea pig and various strains of mice (including syngeneic OD), but not to SC, whether resting or activated, nor to astrocytes, kidney cells or Langerhans islets [16].

Anatomical context of IL2

• Collectively, the data indicate that ascorbic acid selectively influences the proliferation of B lymphocytes and negatively acts on IL2 production by T lymphocytes when a threshold of saturation is exceeded [17].
• Maturation of the mitogen responsiveness, and IL2 and IL6 production by neonatal swine leukocytes [18].
• Among the immunized groups, inoculation with VPIL-6 induced the highest content of IgG (p < 0.05), the greatest bioactivity of IL-2 and the greatest proliferation of lymphocytes from the spleen of the mice [19].
• This suppressive activity is not abrogated by addition of interleukin 2, suggesting that these suppressor cells do not inhibit the proliferation by absorption of the released lymphokine or by inhibition of its production [20].
• Interleukin-2 receptor/p55(Tac)-inducing activity in porcine follicular fluids [21].

Associations of IL2 with chemical compounds

• Recombinant porcine IFN gamma also reversed the immunosuppressive effects of dexamethasone on: i), lymphocyte responsiveness to mitogens: PHA (P less than 0.03), ConA (P less than 0.053); ii), IL1 production; and iii), IL2 production (P less than 0.05) [22].
• Drug-induced lymphocyte proliferation in the experimental sulbenicillin (SBPC) rash of guinea pigs and the enhancement effects of interleukin 2 (IL2) [23].
• In contrast, PGE2 exhibited highly suppressive effects on the cytotoxic activity of both freshly isolated and IL-2-stimulated endometrial effector cells [24].
• Specific tumor memory induced by polyethylene-glycol-modified interleukin-2 requires both helper and cytotoxic T cells [25].
• It is suggested that the potentiating effect of bleomycin is not on suppressor cell function, as with cyclophosphamide, but might have an effect on local interleukin 2 production, particularly if given when T-cell proliferation is on the increase [26].

Other interactions of IL2

• Blood leukocytes produced large quantities of IL6 by 1-2 weeks after birth and IL2 by 2-3 weeks [18].
• PBMC cultures demonstrated peak expression of IL-2, IL-6, IL-8 and IL-10 mRNA levels at 12, 24, 24 and 6h, respectively [27].
• IL-2 production peaked at about 24 h of in vitro culture after Con A activation, but it lasted for 2-3 days after PWM activation [15].
• For IL2, IL4, IL8 or transforming growth factor beta, no differences were seen among groups [28].
• Blood samples were collected on days 15 and 29 to determine peripheral blood lymphocyte proliferation, interleukin-1beta (IL-1beta) and interleukin-2 (IL-2) production [29].

Analytical, diagnostic and therapeutic context of IL2

• Expression of IL-2 mRNA was up-regulated after HTK perfusion and was highest at the start and 7 hours after reperfusion [30].
• Porcine interleukin 2 (IL-2) cDNA was cloned by polymerase chain reaction (PCR), using primers derived from the corresponding bovine sequence [14].
• BCG vaccination of nutritionally normal guinea pigs, on the other hand, induced significantly more IL-2 production by PPD- and concanavalin A-stimulated lymphocytes [4].
• Additionally, 21-day imiquimod treatment enhanced HSV antigen-specific interleukin 2 production and proliferative responses by mononuclear cells (P < 0.001) for 4 weeks after treatment [31].
• Local polyethylene-glycol (PEG)-modified interleukin-2 (IL-2) immunotherapy of the guinea pig Line 10 (L10) tumor was previously demonstrated to evoke long-lasting systemic immunity after cure of the tumor and metastases [25].

References