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Gene Review

poxB  -  pyruvate dehydrogenase

Escherichia coli UTI89

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Disease relevance of poxB


High impact information on poxB


Chemical compound and disease context of poxB


Biological context of poxB

  • Genetic and biochemical analyses of the mutant revealed the downregulation of many TCA cycle enzymes, including citrate synthase, and the upregulation of the pyruvate dehydrogenase complex in both transcription and enzyme activities [16].
  • The presence of PYC in a poxB mutant resulted in a 42% lower maintenance coefficient and a 42% greater biomass yield compared to the wildtype [17].
  • Furthermore, the influence of the poxB gene knockout on the growth of E. coli was also studied because of its similar function to PDHc which connects the glycolysis to the TCA cycle [18].
  • The entire nucleotide sequence of the poxB (pyruvate oxidase) gene of Escherichia coli K-12 has been determined by the dideoxynucleotide (Sanger) sequencing of fragments of the gene cloned into a phage M13 vector [19].
  • The promoter region of poxB, the structural gene for pyruvate oxidase, which is also under the control of sigma S and cAMP-CRP, is very similar to the corresponding region of csiE, suggesting a similar regulatory mechanism also for poxB [20].

Anatomical context of poxB

  • A new metabolic link. The acyl carrier protein of lipid synthesis donates lipoic acid to the pyruvate dehydrogenase complex in Escherichia coli and mitochondria [21].
  • A substantial fraction of the E2 protein as well as part of the pyruvate dehydrogenase enzymatic activity was associated with the cytoplasmic membrane in A. laidlawii [22].
  • Tissue-specific and developmental-specific expression of an Arabidopsis thaliana gene encoding the lipoamide dehydrogenase component of the plastid pyruvate dehydrogenase complex [23].
  • Induced genes were numerous in groups specifying the degradation of small molecules (carbon compounds, amino acids and fatty acids), energy metabolism (aerobic and anaerobic respiration, pyruvate dehydrogenase and TCA cycle), chemotaxis and mobility, flagella biosynthesis, surface structures and phage related functions [24].
  • It is preceded by the gene encoding the pyruvate dehydrogenase component (E1) of pyruvate dehydrogenase complex and by an intercistronic region of 11 base pairs containing a good ribosome binding site [25].

Associations of poxB with chemical compounds

  • Reassociation to a fully active complex molecule works with equal facility between the pyruvate dehydrogenase component and a dihydrolipoamide transacetylase: dihydrolipoamide dehydrogenase subcomplex from either organism in all possible combinations [1].
  • Strain YYC202 (aceEF pfl poxB pps) generated 90 g/liter lactate in 16 h during the anaerobic phase (with a yield of 0.95 g/g and a productivity of 5.6 g/liter . h) [26].
  • The malic enzyme, involved in the production of pyruvate, and pyruvate dehydrogenase, required for the channelling of pyruvate into acetyl-CoA, were also induced in IAA-treated cells [27].
  • Inactivation of poxB caused a severe reduction in growth rate in strain PB11 when grown in the fermentor with medium containing glucose or glucose plus acetate, whereas under the same conditions poxB(-)derivative strains of JM101 and PB12 were not affected [28].
  • The purified enzyme was found to cleave both small molecule lipoylated and biotinylated substrates as well as lipoic acid from two 2-oxoacid dehydrogenases and an isolated lipoylated lipoyl domain derived from the pyruvate dehydrogenase E2 subunit [5].

Analytical, diagnostic and therapeutic context of poxB


  1. Purification and properties of the pyruvate dehydrogenase complex from Salmonella typhimurium and formation of hybrids with the enzyme complex from Escherichia coli. Seckler, R., Binder, R., Bisswanger, H. Biochim. Biophys. Acta (1982) [Pubmed]
  2. Characterization of poxB, a chromosomal-encoded Pseudomonas aeruginosa oxacillinase. Kong, K.F., Jayawardena, S.R., Del Puerto, A., Wiehlmann, L., Laabs, U., Tümmler, B., Mathee, K. Gene (2005) [Pubmed]
  3. HLA DRB4 0101-restricted immunodominant T cell autoepitope of pyruvate dehydrogenase complex in primary biliary cirrhosis: evidence of molecular mimicry in human autoimmune diseases. Shimoda, S., Nakamura, M., Ishibashi, H., Hayashida, K., Niho, Y. J. Exp. Med. (1995) [Pubmed]
  4. Expression in Escherichia coli of genes encoding the E1 alpha and E1 beta subunits of the pyruvate dehydrogenase complex of Bacillus stearothermophilus and assembly of a functional E1 component (alpha 2 beta 2) in vitro. Lessard, I.A., Perham, R.N. J. Biol. Chem. (1994) [Pubmed]
  5. Expression cloning and demonstration of Enterococcus faecalis lipoamidase (pyruvate dehydrogenase inactivase) as a Ser-Ser-Lys triad amidohydrolase. Jiang, Y., Cronan, J.E. J. Biol. Chem. (2005) [Pubmed]
  6. Repeating functional domains in the pyruvate dehydrogenase multienzyme complex of Escherichia coli. Packman, L.C., Hale, G., Perham, R.N. EMBO J. (1984) [Pubmed]
  7. Nucleotide sequence for yeast dihydrolipoamide dehydrogenase. Browning, K.S., Uhlinger, D.J., Reed, L.J. Proc. Natl. Acad. Sci. U.S.A. (1988) [Pubmed]
  8. A computer model analysis of the active-site coupling mechanism in the pyruvate dehydrogenase multienzyme complex of Escherichia coli. Hackert, M.L., Oliver, R.M., Reed, L.J. Proc. Natl. Acad. Sci. U.S.A. (1983) [Pubmed]
  9. Enzyme inhibitory autoantibodies to pyruvate dehydrogenase complex in primary biliary cirrhosis differ for mammalian, yeast and bacterial enzymes: implications for molecular mimicry. Teoh, K.L., Mackay, I.R., Rowley, M.J., Fussey, S.P. Hepatology (1994) [Pubmed]
  10. Autoantibodies in primary biliary cirrhosis: analysis of reactivity against eukaryotic and prokaryotic 2-oxo acid dehydrogenase complexes. Fussey, S.P., Lindsay, J.G., Fuller, C., Perham, R.N., Dale, S., James, O.F., Bassendine, M.F., Yeaman, S.J. Hepatology (1991) [Pubmed]
  11. Pleiotropic effects of poxA regulatory mutations of Escherichia coli and Salmonella typhimurium, mutations conferring sulfometuron methyl and alpha-ketobutyrate hypersensitivity. Van Dyk, T.K., Smulski, D.R., Chang, Y.Y. J. Bacteriol. (1987) [Pubmed]
  12. Purification and primary amino acid sequence of the L subunit of glycine decarboxylase. Evidence for a single lipoamide dehydrogenase in plant mitochondria. Turner, S.R., Ireland, R., Rawsthorne, S. J. Biol. Chem. (1992) [Pubmed]
  13. Yeast TKL1 gene encodes a transketolase that is required for efficient glycolysis and biosynthesis of aromatic amino acids. Sundström, M., Lindqvist, Y., Schneider, G., Hellman, U., Ronne, H. J. Biol. Chem. (1993) [Pubmed]
  14. Regulatory properties of the pyruvate dehydrogenase complex from Escherichia coli. Studies on the thiamin diphosphate-dependent lag phase. Horn, F., Bisswanger, H. J. Biol. Chem. (1983) [Pubmed]
  15. Pyruvate dehydrogenase complex of Escherichia coli. Thiamin pyrophosphate and NADH-dependent hydrolysis of acetyl-CoA. CaJacob, C.A., Gavino, G.R., Frey, P.A. J. Biol. Chem. (1985) [Pubmed]
  16. Alterations of Cellular Physiology in Escherichia coli in Response to Oxidative Phosphorylation Impaired by Defective F1-ATPase. Noda, S., Takezawa, Y., Mizutani, T., Asakura, T., Nishiumi, E., Onoe, K., Wada, M., Tomita, F., Matsushita, K., Yokota, A. J. Bacteriol. (2006) [Pubmed]
  17. Physiological response of central metabolism in Escherichia coli to deletion of pyruvate oxidase and introduction of heterologous pyruvate carboxylase. Vemuri, G.N., Minning, T.A., Altman, E., Eiteman, M.A. Biotechnol. Bioeng. (2005) [Pubmed]
  18. Effect of lpdA gene knockout on the metabolism in Escherichia coli based on enzyme activities, intracellular metabolite concentrations and metabolic flux analysis by 13C-labeling experiments. Li, M., Ho, P.Y., Yao, S., Shimizu, K. J. Biotechnol. (2006) [Pubmed]
  19. Nucleotide sequence and deduced amino acid sequence of Escherichia coli pyruvate oxidase, a lipid-activated flavoprotein. Grabau, C., Cronan, J.E. Nucleic Acids Res. (1986) [Pubmed]
  20. Regulatory characteristics and promoter analysis of csiE, a stationary phase-inducible gene under the control of sigma S and the cAMP-CRP complex in Escherichia coli. Marschall, C., Hengge-Aronis, R. Mol. Microbiol. (1995) [Pubmed]
  21. A new metabolic link. The acyl carrier protein of lipid synthesis donates lipoic acid to the pyruvate dehydrogenase complex in Escherichia coli and mitochondria. Jordan, S.W., Cronan, J.E. J. Biol. Chem. (1997) [Pubmed]
  22. Identification and analysis of the genes coding for the putative pyruvate dehydrogenase enzyme complex in Acholeplasma laidlawii. Wallbrandt, P., Tegman, V., Jonsson, B.H., Wieslander, A. J. Bacteriol. (1992) [Pubmed]
  23. Tissue-specific and developmental-specific expression of an Arabidopsis thaliana gene encoding the lipoamide dehydrogenase component of the plastid pyruvate dehydrogenase complex. Drea, S.C., Mould, R.M., Hibberd, J.M., Gray, J.C., Kavanagh, T.A. Plant Mol. Biol. (2001) [Pubmed]
  24. Gene expression analysis of the response by Escherichia coli to seawater. Rozen, Y., Larossa, R.A., Templeton, L.J., Smulski, D.R., Belkin, S. Antonie Van Leeuwenhoek (2002) [Pubmed]
  25. The dihydrolipoyltransacetylase component of the pyruvate dehydrogenase complex from Azotobacter vinelandii. Molecular cloning and sequence analysis. Hanemaaijer, R., Janssen, A., de Kok, A., Veeger, C. Eur. J. Biochem. (1988) [Pubmed]
  26. Homolactate Fermentation by Metabolically Engineered Escherichia coli Strains. Zhu, Y., Eiteman, M.A., Dewitt, K., Altman, E. Appl. Environ. Microbiol. (2007) [Pubmed]
  27. Indole-3-acetic acid regulates the central metabolic pathways in Escherichia coli. Bianco, C., Imperlini, E., Calogero, R., Senatore, B., Pucci, P., Defez, R. Microbiology (Reading, Engl.) (2006) [Pubmed]
  28. Role of pyruvate oxidase in Escherichia coli strains lacking the phosphoenolpyruvate:carbohydrate phosphotransferase system. Flores, N., de Anda, R., Flores, S., Escalante, A., Hernández, G., Martínez, A., Ramírez, O.T., Gosset, G., Bolívar, F. J. Mol. Microbiol. Biotechnol. (2004) [Pubmed]
  29. Quaternary structure of pyruvate dehydrogenase complex from Escherichia coli. Yang, H.C., Hainfeld, J.F., Wall, J.S., Frey, P.A. J. Biol. Chem. (1985) [Pubmed]
  30. Molecular cloning of the p45 subunit of pyruvate dehydrogenase kinase. Popov, K.M., Kedishvili, N.Y., Zhao, Y., Gudi, R., Harris, R.A. J. Biol. Chem. (1994) [Pubmed]
  31. A library of monoclonal antibodies to Escherichia coli K-12 pyruvate dehydrogenase complex. Competitive epitope mapping studies. McNally, A.J., Mattsson, L., Jordan, F. J. Biol. Chem. (1995) [Pubmed]
  32. Protein-protein interaction revealed by NMR T(2) relaxation experiments: the lipoyl domain and E1 component of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus. Howard, M.J., Chauhan, H.J., Domingo, G.J., Fuller, C., Perham, R.N. J. Mol. Biol. (2000) [Pubmed]
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