Disease relevance of Lenep

• Five lineages produced 'squinting' transgenic mice characterized by microphthalmic eyes with severe lens and retinal dysplasia, and four lineages produced 'bulged' transgenic mice with eyes that exhibited enlarged globes, lens epithelial hyperplasia, anterior segment dysgenesis, and in some cases retinal dysplasia [1].
• Peroxide toxicity in conditioned lens epithelial cells--evaluation of multi-defense systems [2].
• Human cataracts may be congenital or appear later in life; nuclear, cortical and lens epithelial proliferation forms have all been described [3].
• Activated Ras induces lens epithelial cell hyperplasia but not premature differentiation [4].
• We reported that CPT-11 could induce apoptosis in mouse lens epithelial tumors when it was administered to pregnant alphaT3 mice which developed epithelial cell carcinoma in situ in the lens in the perinatal period. p53-deficient alphaT3 mice were generated to analyze the influence of p53 status on tumor cells under combined chemotherapy [5].

High impact information on Lenep

• Biosynthesis of sulphated macromolecules by rabbit lens epithelium. I. Identification of the major macromolecules synthesized by lens epithelial cells in vitro [6].
• The relationship of this heparan sulphate proteoglycan to the lens epithelial basement membrane is the subject of the following paper [6].
• Consistent with these data, we found no evidence for caspase-induced cleavage of major nuclear subcompartment proteins during late erythropoiesis, in contrast to what has been observed in early erythropoiesis and in lens epithelial and keratinocyte differentiation [7].
• Down-regulation of alphaB-crystallin gene was largely derived from changed lens epithelial cell-derived growth factor activity [8].
• Cdk5 regulates cell-matrix and cell-cell adhesion in lens epithelial cells [9].

Chemical compound and disease context of Lenep

• Lens epithelial cell mRNA. III. Elevated expression of macrophage migration inhibitory factor mRNA in galactose cataracts [10].

Biological context of Lenep

• By methods of subtraction-hybridization of lambda ZAP cDNA libraries, prepared from 4-week-old rat lens epithelial cells (capsule) and lens fiber cells (decapsulated lens), we have isolated a specific cDNA clone whose target mRNA is about 600 b long [11].
• Here we show that partially rescued mgRb:Rb-/- mutant fetuses exhibit ectopic lens epithelial cell proliferation, apoptosis and severe cataract [12].
• Exposure of LE cells to 20, 40, or 100 microM H2O2 for 1 h induces a significant loss of cellular proliferation in cells from old AL mice [13].
• Lens epithelial NF-kappaB kinetics were comparable to those of other tissues, indicating that NF-kappaB may play a role in progression or arrest of lens disorders [14].
• Because abnormal deposition of laminin-1 in the lens BM could influence lens epithelial cell adhesion and fiber cell differentiation, the authors propose that SPARC is important to lens homeostasis through its regulation of lens BM matrix organization [15].

Anatomical context of Lenep

• These data suggest that the LEP503 may be an important lens epithelial cell gene involving the processes of epithelial cell differentiation [16].
• Hydrogen peroxide (H2O2) has been reported to be present at significant levels in the lens and aqueous humor in some cataract patients and suggested as a possible source of chronically inflicted damage to lens epithelial (LE) cells [13].
• Previous studies showed that lens epithelial cells proliferate rapidly in the embryo and that a lens mitogen, most likely derived from the blood, is present in the anterior chamber of the embryonic eye (Hyatt, G. A., and Beebe, D. C., Development 117, 701-709, 1993) [17].
• To establish an "electrical fingerprint" for the gap junction channels between mammalian lens epithelial cells [18].
• The electrical properties of gap junction channels between mammalian lens epithelial cells were virtually identical to those previously reported for transfected cell lines expressing connexin43 [18].

Associations of Lenep with chemical compounds

• Lens epithelial cell mRNA. I. Cloning and sequencing of a messenger RNA with a basic motif/leucine-rich domain specifically expressed in rat lens epithelial cells [11].
• Proliferation rate in vivo was assayed using immunostaining for 5-bromo-2'-deoxyuridine (BrdU) in mouse LE cells after 2-week osmotic pump delivery of BrdU [19].
• The matricellular glycoprotein, secreted protein acidic and rich in cysteine (SPARC), has complex biological activities and is important for lens epithelial cell function and regulation of cataract formation [20].
• Effect of surface coating an acrylic intraocular lens with poly(2-methacryloyloxyethyl phosphorylcholine) polymer on lens epithelial cell line behavior [21].
• To further test the involvement of Egr-1 in selenite-induced cell death, mouse lens epithelial cell line (alpha-TN4 cells) was treated with antisense oligonucleotide for Egr-1 [22].

Regulatory relationships of Lenep

• SPARC and laminin-1 were expressed abundantly in the endoplasmic reticulum (ER) of lens epithelial cells [15].

Other interactions of Lenep

• To examine the possible role of Cdk5 in lens epithelial cell adhesion, we stably transfected N/N1003A rabbit lens epithelial cells with cDNAs for Cdk5 or a dominant-negative mutation, Cdk5-T33 [9].
• CONCLUSIONS: The results of both approaches suggest that lens epithelial cell integrity, cell cycle regulation and lens fiber differentiation are intact in the Fgfr1 deficient lens [23].
• CONCLUSIONS: SPARC affects the secretion and deposition of laminin-1 protein in lens epithelial cells [15].
• This study reveals the selective expression of RhoB in the lens during early eye development and suggests a potential role for this small GTPase in cytoskeletal reorganization associated with lens epithelial cell elongation and differentiation [24].
• Loss of one allele of the p53 gene in the lens epithelial tumor in transgenic mice suppresses apoptosis induced by a topoisomerase I inhibitor (CPT-11) [25].

Analytical, diagnostic and therapeutic context of Lenep

• Northern blot hybridization analysis data showed that the target mRNA was preferentially expressed in the lens epithelial cells; it was not found in the retina or in non-ocular tissues [11].
• Response of lens epithelial cells to hydrogen peroxide stress and the protective effect of caloric restriction [13].
• The double whole cell patch clamp technique was applied to isolated cell pairs obtained from mouse lens epithelium and a continuous cell line of lens epithelial cells derived from the sheep lens (SLE 2.1) [18].
• Viral antigen was detected in vivo by the immunoperoxidase technique in lens fibers and occasionally in lens epithelial cell nuclei at 4 and 5 days postinoculation [26].
• By in situ hybridization analysis, the sense RNA was localized to the LE cells and to the glial cells of the retina, and negligibly to the lens fiber cells [27].

References