Disease relevance of Hspb8

• CONCLUSION: H11 kinase promotes the synthesis of glycogen, an essential fuel for the stressed heart in both conditions of overload and ischemia [1].
• In this study, we tested whether H11 kinase also participates in the metabolic adaptation to cardiac hypertrophy and ischemia [1].
• Under continuous heat-stress conditions, the level of HSP22 remained high [2].
• Biodistribution study demonstrated that specific uptake of 125I-hMcAb H11 by human colon cancer xenografts was significantly higher than by normal tissues [3].
• In this study, a recombinant single-chain Fv (scFv) fragment of H11 labelled with 111In was investigated for tumour imaging in athymic mice implanted subcutaneously with A-375 human melanoma xenografts [4].

High impact information on Hspb8

• The transition from constitutive to basal activity (androstenol bound) appears to be associated with a ligand-induced kink between helices H10 and H11 [5].
• According to the two-hybrid data, HSP22 interacts preferentially with the triple aspartate form of HSP27 as compared with wild-type HSP27 [6].
• In studies using monoclonal antibodies to cell-surface antigens we have identified 2 new antigens (H11 and 7.2) expressed on mouse NK cells [7].
• MCF-7/H11 cell hybrids, containing chromosome 11, showed in vitro characteristics similar to the parental cell line [8].
• Upon relief of heat stress, the HSP22 proteins disappeared with a half-life of about 4 h and were undetectable after 21 h of recovery [2].

Chemical compound and disease context of Hspb8

• OBJECTIVE: H11 kinase is a serine/threonine kinase preferentially expressed in the heart, which participates in cardiac cell growth and also in cytoprotection during ischemia [1].

Biological context of Hspb8

• Therefore, H11 kinase represents an integrative sensor in the cardiac adaptation to stress by coordinating cell growth, survival and metabolism [1].
• Glycogen content was increased proportionately to the expression of the transgene, reaching a 40% increase in high-expression transgenic mice (7-fold increase in H11 kinase protein) versus wild type (p < 0.01) [1].
• A cDNA for maize mitochondrial HSP22 was cloned and extended to full length with sequences from an expressed sequence tag database [2].
• The up-regulation of HSP70 and HSP22 appeared specific towards proteasome inhibitor-mediated cell death [9].
• Genes that were differentially up-regulated at the early time point but not when most cells were undergoing apoptosis might be involved in an attempt to reverse proteasome inhibitor-mediated apoptosis and include HSP70, HSP22 and cell cycle inhibitors [9].

Anatomical context of Hspb8

• Maximal HSP22 expression occurred in etiolated seedling mitochondria after 5 h of a +13 degrees C heat stress [2].
• Four antibodies (126-4, 5.1 H11, UJ127.11, and 14.G2a) all reacted with median levels of less than 2% (range 0.0 to 5.4) of CD34+ stem cells (median of 14 patients) [10].
• The hybridomas capable of secreting human monoclonal antibodies were screened by using human colon cancer cell lines and pathological biopsies with ELISA and immunohistochemical methods. hMcAb clone H11 was selected for a large-scale antibody production, which was purified from mouse ascites [3].
• H11 is a human IgM monoclonal antibody which recognizes a novel tumour-associated antigen expressed on melanoma, glioma, breast cancer, colon cancer, prostate cancer, lung cancer and B-cell lymphoma [4].
• We tested the role of the protein H11 kinase (H11K), which accumulates by 4- to 6-fold in myocardium of patients with chronic ischemic heart disease and in experimental models of ischemia [11].

Associations of Hspb8 with chemical compounds

• METHODS AND RESULTS: A yeast two-hybrid screen using H11 kinase as a bait in a human heart library revealed a potential interaction with phosphoglucomutase (PGM), the enzyme converting glucose 6-phosphate into glucose 1-phosphate [1].
• A murine IgM monoclonal antibody (MAb H11) was developed against the type polysaccharide capsular antigen of group B streptococcus (GBS), serotype IV, after intraperitoneal immunisation of BALB/c mice with heat-killed bacteria [12].
• H11 scFv was derivatized with diethylenetriaminepentaacetic acid (DTPA) for labelling with 111In [4].
• H11 also reacted with poly (glycosyl) ceramide purified from group 0 red cells and glycoprotein H purified from human stomach mucosa and meconium [13].

Other interactions of Hspb8

• Monoclonal antibodies were developed to maize HSP70, cpn60, and HSP22 [2].

Analytical, diagnostic and therapeutic context of Hspb8

• A cardiac-specific transgenic mouse overexpressing H11 kinase (2- to 7-fold protein increase) has been generated, and is characterized by cardiac hypertrophy with preserved function and protection against irreversible damage during ischemia/reperfusion [1].
• Interaction between H11 kinase and PGM was confirmed by co-immunoprecipitation [1].
• Sequence analysis indicated that HSP22 is a member of the plant small heat-shock protein superfamily [2].
• The development of human monoclonal antibodies such as hMcAb H11 may be useful for radioimmunodetection and therapy of colon cancer [3].
• MAb H11 reacted in immunodiffusion with the purified polysaccharide in both its sialylated and desialylated form, giving a line of identity, and opsonised type IV GBS strains in an in vitro assay [12].

References