Disease relevance of TRICHLOROACETIC ACID

• Isolation and purification of plasmids from Bacteroides fragilis using rubidium trichloroacetate density gradient centrifugation [1].
• Previous studies have shown that dichloroacetate and trichloroacetate increase the toxicity of CHCl3 [2].

High impact information on TRICHLOROACETIC ACID

• We have dissociated the F0 complex by treatment with trichloroacetate (3 M) at pH 8.0, in the presence of deoxycholate (1%) and N-tetradecyl-N, N-dimethyl-3-ammonio-1-propanesulfonate (Zwittergent 3-14, 5%) [3].
• Isopycnic centrifugation of vacuoles in 2.25 M sorbitol-Metrizamide density gradients yielded a peak (density, 1.31 g/cm3) of vacuolar markers coincident with 32P-phospholipids, trichloroacetate-insoluble 14C, and trichloroacetate-soluble 14C [4].
• Expression of the putative cyclase catalytic region (HCAT) resulted in the formation of an active enzyme that migrated as a homodimer on gel filtration columns; treatment with sodium trichloroacetate caused dissociation of the dimer and a loss of cyclase activity [5].
• Alkaline phosphatase has been purified from microsomes of chicken epiphyseal cartilage by first selectively extracting certain adventitious proteins with 0.25 M trichloroacetate [6].
• The properties of native and denatured DNA in buoyant rubidium trichloroacetate at neutral pH [7].

Biological context of TRICHLOROACETIC ACID

• The reaction of the cyclic tetrathiadiynes 4(m.n) (where m and n indicate the number of methylene groups between the dithiaacetylene units) with sodium trichloroacetate and subsequent hydrolysis of the gem-dichlorocyclopropenes afforded the mono- and biscyclopropenone derivatives 5(m.n) and 6(m.n) in moderate yields [8].
• In vivo kinetics of trichloroacetate in male Fischer 344 rats [9].
• Although the area under the curve values for serum trichloroacetate were slightly greater in the dietary controlled and calorically restricted groups than in the ad libitum-fed groups, this increase did not appear to completely account for the potentiation of hepatic enzyme induction by dietary restriction [10].
• Protein-binding assay for cyclic AMP: possible interference by traces of trichloroacetate [11].
• Differences in phenotype and cell replicative behavior of hepatic tumors induced by dichloroacetate (DCA) and trichloroacetate (TCA) [12].

Anatomical context of TRICHLOROACETIC ACID

• Trapping and identification of the dichloroacetate radical from the reductive dehalogenation of trichloroacetate by mouse and rat liver microsomes [13].
• Application of cryopreserved human hepatocytes in trichloroethylene risk assessment: relative disposition of chloral hydrate to trichloroacetate and trichloroethanol [14].
• A decreased sensitivity of human PPARalpha compared to mouse PPARalpha to trans-activation was observed with some (Wy-14, 643, PFOA), but not other, peroxisome proliferators (TCE metabolites, trichloroacetate and dichloroacetate; and DEHP metabolites, mono[2-ethylhexyl]phthalate and 2-ethylhexanoic acid) [15].
• Tri and the metabolites examined, except trichloroacetate and DCVG, caused a loss of function of liver and kidney mitochondria [16].
• After incubation with guanidine-thiocyanate, trichloroacetate, and phenol, the immunoreactivity of kuru plaques and senile plaques was enhanced to the same level as the formic acid treatment [17].

Associations of TRICHLOROACETIC ACID with other chemical compounds

• To further characterize the molten globule states, we examined by far-UV circular dichroism the denaturant-induced unfolding transitions of the molten globule states stabilized by sodium chloride or sodium trichloroacetate [18].
• Treatment of the latter with sodium trichloroacetate followed by ammonium sulfate fraction results in separation of the 49000 molecular weight polypeptide from the two smaller subunits [19].
• Photodeposition of platinum nanoparticles on dye-sensitized TiO2 (Pt/TiO2/Ru(II)L3) drastically enhanced the degradation rate of TCA and CCl4 [20].
• The reaction of chloroplast fructose-1,6-bisphosphatase with dithiothreitol was accelerated 13-fold by the chaotropic anion: second-order rate constants were 48.1 M-1.min-1 and 3.7 M-1.min-1 in the presence and in the absence of trichloroacetate, respectively [21].
• The regioselective introduction of the non-conjugated double-bond into the six-membered ring was then achieved by esterification of the 4-hydroxyl group using trichloroacetic acid to give the trichloroacetate (-)-69 [22].

Gene context of TRICHLOROACETIC ACID

• Exposure of hepatocytes to Wy-14,643 and trichloroacetate resulted in a dose-dependent phosphorylation and activation of ERK [23].
• Role of the peroxisome proliferator-activated receptor alpha (PPARalpha) in responses to trichloroethylene and metabolites, trichloroacetate and dichloroacetate in mouse liver [24].
• Replacement of KCl by KBr, potassium acetate or potassium trichloroacetate in the K(+)-rich medium resulted in different relationships between cell volume and the rate of detritiation, in agreement with the differential effect of these salts on the activity of purified glucokinase assayed in the presence of regulatory protein [25].
• Both DCA and TCA differ in their hepatic effects; TCA produces peroxisome proliferation as measured by increases in cyanide-insensitive acyl CoA oxidase activity, whereas DCA increases glycogen concentrations [26].
• 3. Ubiquitin was isolated in relatively high yield from the TCA insoluble fraction by use of single ion-exchange chromatographic and gel permeation steps [27].

Analytical, diagnostic and therapeutic context of TRICHLOROACETIC ACID

• The subunits were separated by gel filtration with trichloroacetate (1 M) included in the elution buffer [3].

References