The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

ETV3  -  ets variant 3

Homo sapiens

Synonyms: ETS domain transcriptional repressor PE1, ETS translocation variant 3, METS, Mitogenic Ets transcriptional suppressor, PE-1, ...
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of ETV3

  • Antibody PE1, raised in a separate fusion, was able to select phage clones from a 12mer and 20mer library, revealing that the sequence 187GGFMRG192 is probably the antibody epitope [1].
  • PE-1 is a hexaacylated analog of Escherichia coli lipid A (compound 506) [2].
  • Analysis of the proteins according to a modified technique by Mets and Bogorad (1974) (pH 4.5/pH 8.6 SDS system) revealed 28 and 29 proteins in the small subunits and 37 and 38 proteins in the large subunits of Krebs II ascites and HeLa ribosomes [3].
  • Microarray and real-time PCR experiments showed an overexpression of this serine protease inhibitor in the metastatic tumors (M-4A4T) and its derived metastases (M4-Mets) compared with the weakly metastatic tumors (NM-2C5T), and its release into the blood was confirmed by western-blotting [4].
  • Few studies have evaluated the utility of measuring PE-1 in duodenal fluid for the diagnosis of pancreatic insufficiency [5].

Psychiatry related information on ETV3


High impact information on ETV3

  • Here, we describe a mechanism in which induction of the Ets repressor METS/PE1 links terminal differentiation to cell cycle arrest [8].
  • DNase I protection analysis indicates that oat nuclear extracts contain multiple factors that bind to an array of sequence motifs, including PE1 and part of PE3, within 400 bp upstream of the oat phyA3 transcription start site [9].
  • In contrast, 5' and internal deletions identify a minimum of three major positive promoter elements, designated PE1 [-381 base pairs (bp) to -348 bp], PE2 (-635 bp to -489 bp), and PE3 (-110 bp to -76 bp) that are necessary for high-level expression in low-Pfr cells [10].
  • The solution structure and extended x-ray absorption fine structure data on the former protein reveal that the protein binds copper(I) through a histidine and three Mets in a cupredoxin-like fold [11].
  • We suggest, therefore, that the pO2 clone may encode the putative nuclear factor, oat PF1, that is involved in positive regulation of PHYA3 by binding to PE1 in vivo. pO2 encodes a 170-amino-acid-long protein that contains three repeats of the 'AT-hook' DNA-binding motif found in high mobility group I-Y (HMGI-Y) proteins [12].

Chemical compound and disease context of ETV3


Biological context of ETV3

  • PE-1 was localized to human chromosome 1q21-q23 using both in situ chromosomal hybridization and human-hamster hybrids [15].
  • PE-1, a novel ETS oncogene family member, localizes to chromosome 1q21-q23 [15].
  • However, all three proteins appeared to bind much stronger in south-western assays to two of the rather AT-rich sequences used in our screening than to the PE1 element [16].
  • The dependence of the alkylation extent of the target on the reagent concentration was treated using the equation derived earlier for the two-component system (reagent + target) to calculate association constants of X with P, PE1, PE2 and PE1E2 [17].
  • Similarly, multiple regression analyses between Mets and determinants of cardiovascular function demonstrated significant correlations with Ex/R heart rate, Ex/R count output, and Ex/R A-VO2 in both groups [18].

Anatomical context of ETV3

  • Using macrophages as a model, we provide evidence that METS/PE1 blocks Ras-dependent proliferation without inhibiting Ras-dependent expression of cell type-specific genes by selectively replacing Ets activators on the promoters of cell cycle control genes [8].
  • PE-1 was expressed as an approximately 7.5-kb transcript in most cell lines tested [15].
  • Using partially degenerate oligonucleotides from conserved regions of the ETS domain and the polymerase chain reaction, we isolated a new member of the v-ets family designated PE-1 from HL60 cells [15].
  • At endpoint (9 months), mechanical properties of LV papillary muscles and serum ACE activity were studied in a subgroup of 32 CSH (C, n = 8; PE1, n = 10; and PE2, n = 14) [14].
  • The pathologic stage of these cases, as determined by results from radical prostatectomy, lymphadenectomy, or bone scan, categorized the PCA as either OC, non-OC due to capsular penetration only (NOC-CP) or advanced disease with metastasis (NOC-Mets), i.e., seminal vesicle and/or lymph-node positive or bone-scan positive [19].

Associations of ETV3 with chemical compounds

  • PE-2 differs from PE-1 in carrying two myristic acid residues at GlcN I [2].
  • A Mets motif peptide found in copper transport proteins selectively binds Cu(I) with methionine-only coordination [20].
  • Quadratic response (QR) calculations of spin-orbit coupling and phosphorescence radiative lifetime (tau(R)) indicated a good agreement with experimental tau(R) values reported for solid PE1 and PE2 and PE2 capped with dendrimers in tetrahydrofuran solutions [21].

Other interactions of ETV3


Analytical, diagnostic and therapeutic context of ETV3

  • Chromatin immunoprecipitation experiments demonstrate that CREB-1/CREM-1 are recruited to the PE-1/METS promoter as well as to the promoters of other genes that are up-regulated during terminal macrophage differentiation [22].
  • An electrophoretic mobility shift assay (EMSA) showed specific binding sites for the transcription factors of the oligonucleotides PE1 (-148 to -98) derived from PE [23].
  • PE1 was not associated with the remission induction outcome [24].
  • PE-1 was measured using an ELISA (Genova Diagnostics, Asheville, NC) [5].
  • CONCLUSIONS: The serum PE-1 level correlated closely with the serum amylase but conferred no benefit as a diagnostic test, nor did it provide further prognostic information [25].


  1. Comprehensive epitope analysis of monoclonal anti-proenkephalin antibodies using phage display libraries and synthetic peptides: revelation of antibody fine specificities caused by somatic mutations in the variable region genes. Böttger, V., Böttger, A., Lane, E.B., Spruce, B.A. J. Mol. Biol. (1995) [Pubmed]
  2. Biological activity of synthetic phosphonooxyethyl analogs of lipid A and lipid A partial structures. Ulmer, A.J., Heine, H., Feist, W., Kusumoto, S., Kusama, T., Brade, H., Schade, U., Rietschel, E.T., Flad, H.D. Infect. Immun. (1992) [Pubmed]
  3. Characterisation of ribosomal proteins from HeLa and Krebs II mouse ascites tumor cells by different two-dimensional polyacrylamide gel electrophoresis techniques. Issinger, O.G., Beier, H. Mol. Gen. Genet. (1978) [Pubmed]
  4. Tumor-host interactions contribute to the elevated expression level of alpha1-antichymotrypsin in metastatic breast tumor xenografts. Montel, V., Pestonjamasp, K., Mose, E., Tarin, D. Differentiation (2005) [Pubmed]
  5. Analysis of pancreatic elastase-1 concentrations in duodenal aspirates from healthy subjects and patients with chronic pancreatitis. Stevens, T., Conwell, D., Zuccaro, G., Van Lente, F., Khandwala, F., Hanaway, P., Vargo, J.J., Dumot, J.A. Dig. Dis. Sci. (2004) [Pubmed]
  6. Physical activity patterns of rural Senegalese adolescent girls during the dry and rainy seasons measured by movement registration and direct observation methods. Bénéfice, E., Cames, C. European journal of clinical nutrition. (1999) [Pubmed]
  7. Heidenhain variant of Creutzfeldt-Jakob disease: diffusion-weighted MRI and PET characteristics. Tsuji, Y., Kanamori, H., Murakami, G., Yokode, M., Mezaki, T., Doh-ura, K., Taniguchi, K., Matsubayashi, K., Fukuyama, H., Kita, T., Tanaka, M. Journal of neuroimaging : official journal of the American Society of Neuroimaging. (2004) [Pubmed]
  8. An induced Ets repressor complex regulates growth arrest during terminal macrophage differentiation. Klappacher, G.W., Lunyak, V.V., Sykes, D.B., Sawka-Verhelle, D., Sage, J., Brard, G., Ngo, S.D., Gangadharan, D., Jacks, T., Kamps, M.P., Rose, D.W., Rosenfeld, M.G., Glass, C.K. Cell (2002) [Pubmed]
  9. A negatively acting DNA sequence element mediates phytochrome-directed repression of phyA gene transcription. Bruce, W.B., Deng, X.W., Quail, P.H. EMBO J. (1991) [Pubmed]
  10. cis-acting elements involved in photoregulation of an oat phytochrome promoter in rice. Bruce, W.B., Quail, P.H. Plant Cell (1990) [Pubmed]
  11. A copper(I) protein possibly involved in the assembly of CuA center of bacterial cytochrome c oxidase. Banci, L., Bertini, I., Ciofi-Baffoni, S., Katsari, E., Katsaros, N., Kubicek, K., Mangani, S. Proc. Natl. Acad. Sci. U.S.A. (2005) [Pubmed]
  12. Cloning and characterization of cDNAs encoding oat PF1: a protein that binds to the PE1 region in the oat phytochrome A3 gene promoter. Nieto-Sotelo, J., Quail, P.H. Biochem. Soc. Symp. (1994) [Pubmed]
  13. Antitumor activity of spinasterol isolated from Pueraria roots. Jeon, G.C., Park, M.S., Yoon, D.Y., Shin, C.H., Sin, H.S., Um, S.J. Exp. Mol. Med. (2005) [Pubmed]
  14. Effects of early and late therapy with perindopril on survival and myocardial inotropic state in experimental dilated cardiomyopathy. Chemla, D., Scalbert, E., Desché, P., Lerebours, G., Suard, I., Lecarpentier, Y. J. Cardiovasc. Pharmacol. (1994) [Pubmed]
  15. PE-1, a novel ETS oncogene family member, localizes to chromosome 1q21-q23. Klemsz, M., Hromas, R., Raskind, W., Bruno, E., Hoffman, R. Genomics (1994) [Pubmed]
  16. Novel members of a family of AT hook-containing DNA-binding proteins from rice are identified through their in vitro interaction with consensus target sites of plant and animal homeodomain proteins. Meijer, A.H., van Dijk, E.L., Hoge, J.H. Plant Mol. Biol. (1996) [Pubmed]
  17. Cooperative interactions in the tandem of oligonucleotide derivatives arranged at complementary target. Quantitative estimates and contribution of the target secondary structure. Fedorova, O.S., Adeenah-Zadah, A., Knorre, D.G. FEBS Lett. (1995) [Pubmed]
  18. Determinants of variable exercise performance among patients with severe left ventricular dysfunction. Higginbotham, M.B., Morris, K.G., Conn, E.H., Coleman, R.E., Cobb, F.R. Am. J. Cardiol. (1983) [Pubmed]
  19. Prediction of prostate carcinoma stage by quantitative biopsy pathology. Veltri, R.W., Miller, M.C., Partin, A.W., Poole, E.C., O'Dowd, G.J. Cancer (2001) [Pubmed]
  20. A Mets motif peptide found in copper transport proteins selectively binds Cu(I) with methionine-only coordination. Jiang, J., Nadas, I.A., Kim, M.A., Franz, K.J. Inorganic chemistry. (2005) [Pubmed]
  21. Application of density functional theory for studies of excited states and phosphorescence of platinum(II) acetylides. Minaev, B., Jansson, E., Lindgren, M. The Journal of chemical physics. (2006) [Pubmed]
  22. PE-1/METS, an antiproliferative Ets repressor factor, is induced by CREB-1/CREM-1 during macrophage differentiation. Sawka-Verhelle, D., Escoubet-Lozach, L., Fong, A.L., Hester, K.D., Herzig, S., Lebrun, P., Glass, C.K. J. Biol. Chem. (2004) [Pubmed]
  23. Promoter analysis of interleukin 19. Chen, P.J., Wei, C.C., Wang, C., Chen, F.W., Hsu, Y.H., Chang, M.S. Biochem. Biophys. Res. Commun. (2006) [Pubmed]
  24. Prognostic significance of the blast self-renewal capacity in patients with acute myeloid leukemia. Nara, N., Kurokawa, H., Tanikawa, S., Tomiyama, J., Nagata, K. Cancer (1994) [Pubmed]
  25. A prospective study of serum pancreatic elastase-1 in the diagnosis and assessment of acute pancreatitis. Millson, C.E., Charles, K., Poon, P., Macfie, J., Mitchell, C.J. Scand. J. Gastroenterol. (1998) [Pubmed]
WikiGenes - Universities