Disease relevance of RAG1AP1

• We investigated fusion between phagosomes containing the intracellular pathogen Mycobacterium tuberculosis versus the extracellular pathogen Staphylococcus aureus (designated MCP for mycobacteria-containing phagosome and SCP for S. aureus-containing phagosome) and cytoplasmic compartments in human neutrophils [1].
• We used electron cryomicroscopy and antibody labeling to show that the 8-kDa SCP of human cytomegalovirus is attached only to major capsid protein subunits of the hexons, not the pentons [2].
• In Kaposi's sarcoma-associated herpesvirus (KSHV), the protein encoded by the ORF65 gene (pORF65) is the putative SCP but its location remains controversial due to the absence of such horn-shaped densities from both the pentons and hexons of the KSHV capsid reconstructions [3].
• The lack of horn-shaped densities on the hexons indicates that KSHV SCP exhibits structural features that are substantially different from those of HSV-1 SCP [3].
• A comparable herpes simplex virus type 1 (HSV-1) mutant has recently been generated by fusion of the GFP open reading frame (ORF) with the HSV-1 ORF encoding small capsid protein (SCP) VP26 (P. Desai and S. Person, J. Virol. 72:7563-7568, 1998) [4].

Psychiatry related information on RAG1AP1

• The Stockholm Cancer Prevention Program (SCPP) is a community-based program aimed at reducing cancer incidence and mortality by reducing risk factors related to life-style: dietary habits, tobacco use, and sunbathing [5].
• Mean interdigestive PP plasma concentrations during phase III motor activity were lower in MCP (146 +/- 46 pg/ml) than in controls (270 +/- 42 pg/ml) and lower still in SCP (55 +/- 8 pg/ml) [6].

High impact information on RAG1AP1

• The characteristics of teleost SCPP genes and their expression domains in tooth development suggest the possibility that enameloid arose from dentin and enamel from enameloid more than once in vertebrate evolution [7].
• To determine whether these proteins are associated with protein complexes in the thylakoid membrane, an abundant member of the SCP family, ScpD, was marked with a His tag, and proteins co-isolating with His-tagged ScpD were identified [8].
• Downregulation of Rab5a by antisense oligonucleotides efficiently reduced the synthesis of Rab5a, the binding of syntaxin-4 to MCP and SCP and the capacity for fusion exhibited by the pathogen-containing phagosomes, but it had no effect on bacteria internalisation [1].
• We observed that during phagocytosis, Rab5a in GTP-bound form interacted with syntaxin-4 on the membrane of MCP and were retained for up to 90 minutes, whereas the complex was recruited to the SCP within 5 minutes but was selectively depleted from these vacuoles after 30 minutes of phagocytosis [1].
• Similar to its herpes simplex virus counterpart, VP26 (UL35), the HCMV smallest capsid protein (SCP; UL48/49) decorates hexons in the mature capsid [9].

Chemical compound and disease context of RAG1AP1

• The SCP consisted of Methylomonas methanolica, a pseudomonas species which is an obligate methanol user [10].
• Replacement of the single cysteine residue (C192) with serine in the Streptococcus pyogenes extracellular cysteine protease (SCP) prevented auto-catalytic processing of the 40-kDa zymogen to the 28-kDa mature form and eliminated proteolytic activity [11].

Biological context of RAG1AP1

• CMV genomes with deletion of the SCP ORF also did not give rise to infectious virus [4].
• SCP has four distinct domains with the typical helix-loop-helix (EF-hand) Ca(2+)-binding motif, although the second Ca(2+)-binding domain is not functional due to amino acid changes in the loop [12].
• In contrast to VP26, the HCMV SCP is essential for virus assembly [9].
• Recombinant CMV genomes expressing a fusion protein consisting of GFP and the SCP were constructed by the recently established bacterial artificial chromosome mutagenesis procedure [4].
• In Helix pomatia a 10 kDa sarcoplasmic calcium binding protein I (SCP I)-immunoreactive material was localized immunologically in neurons of the central nervous system, including the electrically silent serotonergic metacerebral giant neuron [13].

Anatomical context of RAG1AP1

• The use of SPPP resulted in a marked rise in GPDH activity compared with the cells cultured with SCP [14].
• SPPP can be considered superior to SCP for the culture of primary human preadipocytes in adipose tissue engineering in terms of proliferation activity and differentiation capacity [14].
• SCP-like and FMRFamide-like immunoreactive material is found in the heart, kidney and pericardium and in the nerves that innervate these organs [15].
• Antisera against sarcoplasmic calcium binding protein II (SCP II) stained a 20 kDa protein in individual muscle cells of the epineurium [13].
• Objective: Understanding the ability of the paraspinal anastomotic network to provide adequate spinal cord perfusion pressure (SCPP) critical for both surgical and endovascular repair of thoracoabdominal aortic aneurysms (TAAA) [16].

Associations of RAG1AP1 with chemical compounds

• After precalcification and soaking in supersaturated Ca-P solution (SCP), calcium phosphate rapidly precipitated onto the surfaces of titanium, and after only three days an uniform apatite layer was found up to thickness of a few micrometers [17].
• All isolates from sugar cane (SCP) could grow up to Cr(VI) concentration of 17mM [18].
• We show that the spinning side-bands of protonated and non-protonated carbon atoms can be well separated by means of the standard SCP and LCPD experiments at a relatively slow sample spinning rate or at high magnetic field [19].
• Consequently, the soil sorption of three antimicrobial agents--sulfachloropyridazine (SCP), tylosin (TYL), and oxytetracycline (OTC)--was investigated [20].
• Whole native straw was found to be the most recalcitrant to bioconversion into SCP; however, protein production was almost doubled when the lignin component was removed using a mixture of sodium chlorite and acetic acid [21].

Analytical, diagnostic and therapeutic context of RAG1AP1

• The advantages of spectral subtraction techniques were demonstrated for separation of SCP ROA signals when multiple chiral entities are present [22].
• METHODS: From June 1997, 60 consecutive patients who underwent total arch replacement through a midsternotomy were alternately allocated to one of two methods of brain protection: deep hypothermic circulatory arrest with retrograde cerebral perfusion (RCP: 30 patients) or with selective antegrade cerebral perfusion (SCP: 30 patients) [23].
• During the last step of purification (DEAE-cellulose chromatography), SCP emerges in three peaks in the ratio of 14:1.5:1 for crayfish, of 7:2:1 for lobster and of 3:2:1 for shrimp [24].
• The ECG signal is acquired in SCP format [25].
• In its work, SCPP utilizes a community-based approach focusing on (1) reduction in tobacco consumption, (2) reduction in dietary fat consumption and increase in dietary fiber consumption, and (3) changing sunbathing behavior in order to reduce the incidence of malignant melanoma [26].

References