Disease relevance of BNIP3L

• Exogenous expression of BNIP3, but not BNIP3L, induced autophagic cell death in malignant glioma cells without As(2)O(3) treatment [1].
• Analysis by cDNA microarray, RT-PCR, and Western blot showed that cell death members of Bcl-2 family, Bcl-2/adenovirus E1B 19-kDa-interacting protein 3 (BNIP3) and its homologue BNIP3-like (BNIP3L), were upregulated in As(2)O(3)-induced autophagic cell death [1].
• Using cDNA arrays and serial analysis of gene expression, we found that hypoxia induces up-regulation of BNIP3 and its homologue, Nip3-like protein X. Analysis of human carcinoma cell lines showed that they are hypoxically regulated in many tumor types, as well as in endothelial cells and macrophages [2].
• Analysis of the candidate 8p21 tumour suppressor, BNIP3L, in breast and ovarian cancer [3].
• These data suggest that BNIP3L is unlikely to be the target of 8p LOH in ovarian or breast cancer [3].

High impact information on BNIP3L

• Moreover, TSAP6 enhanced the susceptibility of cells to apoptosis and cooperated with Nix to exacerbate this effect [4].
• This study demonstrates a direct link between HIF-1alpha and a proapoptotic member of the Bcl-2 family and offers a reasonable physiological function for members of the Bcl-2 subfamily, including Nip3 and its close relative Nix [5].
• Polymerase chain reaction (PCR)-based transcriptional patterning confirmed the increased expression of Nix during human erythropoiesis with a pattern similar to that of Bcl-xL and glycophorin A and opposite that of Bcl-2 [6].
• The proapoptotic factor Nix is coexpressed with Bcl-xL during terminal erythroid differentiation [6].
• Our in silico screening strategy indicated that a hypoxia-inducible proapoptotic member of the Bcl-2 gene family called Nix is expressed during erythropoiesis [6].

Chemical compound and disease context of BNIP3L

• Past work showed induction of Nix transcripts in a cellular model of Parkinson disease based on neuronal PC12 cells exposed to 6-hydroxydopamine [7].
• Single application of Nix (permethrin 1% creme rinse) and Rid were compared as treatments for Pediculosis capitis in 435 patients, the majority of whom were children [8].

Biological context of BNIP3L

• The gene, designated BNIP3L, was expressed in all 16 normal human tissues examined; we mapped it to chromosome band 8p21 by fluorescence in situ hybridization [9].
• EGR2 induces apoptosis in various cancer cell lines by direct transactivation of BNIP3L and BAK [10].
• HIF-1-dependent regulation of hypoxic induction of the cell death factors BNIP3 and NIX in human tumors [2].
• Downregulation of hypoxic BNIP3 in CRC cells was independent of the expression of other BCL-2 family members, or BNIP3L [11].
• Genetic analysis of BNIP3L in 40 primary ovarian and 25 primary breast tumours identified one somatic, intronic mutation in one ovarian tumour, as well as several polymorphisms, including one resulting in an amino-acid substitution [3].

Anatomical context of BNIP3L

• BNIP3L expression was assessed by both RT-PCR and Northern blot analysis in breast and ovarian cancer cell lines and found to be expressed at similar levels relative to expression in their respective normal epithelial cell lines [3].
• We next performed Northern blot analyses and determined that the 1.4-kb Nix transcript is expressed at lower levels in erythroleukemia cells than reticulocytes [6].
• Expression of s-Nix, a dominant-negative form of Nix, protects neuronal PC12 cells from 6-hydroxydopamine but not from nerve growth factor deprivation [7].
• The expression of rat NIP3L identified in this study increased slightly in the pancreas after induction of acute pancreatitis but showed a marked increase in the lung by both Northern and Western blot analysis [12].
• An intranuclear pathogen [nuclear inclusion X (NIX)] associated with massive mortalities of the Pacific razor clam, Siliqua patula [13].

Associations of BNIP3L with chemical compounds

• Nix, a pro-apoptotic BH3-only protein, promotes apoptosis of non-neuronal cells, although the mechanisms involved remain incompletely understood [7].
• These can serve to organize cofactor interactions with biomolecules, as was recently demonstrated for a biotin-selective RNA aptamer that depends on a direct biotin-magnesium interaction for recognition of biotin (Nix, J.; Sussman, D.; Wilson, C. J. Mol. Biol. 2000, 296, 1235-1244) [14].
• An observational, epidemiological study was undertaken to evaluate the safety of permethrin 1% creme rinse (Nix) for treatment of head lice infestations [15].
• OBJECTIVE: To compare the pediculicidal activity of Ovide lotion and its active ingredient, 0.5% malathion, with Nix and its active ingredient, 1% permethrin, in permethrin-resistant head lice [16].
• We treated 53 men with the diagnosis of PP with either 1% lindane (Kwell) shampoo for four minutes or 1% permethrin (Nix) creme rinse for ten minutes, according to random assignment [17].

Other interactions of BNIP3L

• Isolation, mapping, and functional analysis of a novel human cDNA (BNIP3L) encoding a protein homologous to human NIP3 [9].
• Analysis by cDNA microarray and subsequent functional studies revealed that EGR2 directly transactivates expression of BNIP3L and BAK [10].
• The p53-inducible TSAP6 gene product regulates apoptosis and the cell cycle and interacts with Nix and the Myt1 kinase [4].

Analytical, diagnostic and therapeutic context of BNIP3L

• The placental expression of BNip3/Nix has been investigated with immunohistochemistry by the use of specific human BNip3/Nix antibodies [18].
• Co-immunoprecipitation and in vitro binding studies confirmed a direct interaction between POSH and Nix in mammalian cells [7].
• Computational sequence analysis techniques, BLAST and NIX, were used to assemble a physical map of MRIII, consisting of three overlapping bacterial artificial chromosome (BAC) clones, 297N7, 963H4 and 795F17, from the RPCI-11 library [19].
• The percentage length of the file visible was significantly less for Sens-A-Ray (P=0.02) and for Nix NF45-100 film (P<0.005) when compared with D-speed film processed with conventional chemistry [20].

References