Disease relevance of Nt5c3

• Cloning and expression analysis of murine lupin, a member of a novel gene family that is conserved through evolution and associated with Lupus inclusions [1].
• Mice that were fed the transgenic lupin tissue developed significant levels of hepatitis B virus-specific antibodies [2].
• A single i.p. injection of BHT (400 mg/kg body weight) decreased p36 phosphorylation severalfold in both BALB/cByJ and A/J mice [3].
• Therefore, the DNA fragment encoding hepatitis B virus surface antigen was introduced into Agrobacterium tumerifacience LBA4404 and used to obtain transgenic lupin (Lupinus luteus L.) and lettuce (Lactuca sativa L.) cv. Burpee Bibb expressing envelope surface protein [2].
• Structure of pyrimidine 5'-nucleotidase type 1. Insight into mechanism of action and inhibition during lead poisoning [4].

Psychiatry related information on Nt5c3

• Free PNI protein is markedly decreased in various anatomical brain regions, including hippocampus, of patients with Alzheimer disease [5].

High impact information on Nt5c3

• In contrast, protein kinase C (PKC)-dependent serine and tyrosine phosphorylations were stimulated maximally by low level occupancy of PDGF binding sites, and phosphorylation of p36 required high occupancy [6].
• We postulate that p36 MBP kinase is a common component in diverse signaling pathways leading to apoptosis [7].
• We also demonstrate the activation of p36 kinase in tumor necrosis factor-alpha- and serum starvation-induced cell death using the human prostatic tumor cell line LNCap and NIH 3T3 fibroblasts, respectively [7].
• Protease nexin I (PNI) is a member of the family of serine protease inhibitors (serpins) that have been shown to promote neurite outgrowth in vitro from different neuronal cell types [5].
• Because 24 h infusion of a high dose of DPI is tolerated and inhibits Ki-Ras prenylation, we compared the antitumor efficacy from a 24-h FTI infusion to that of a DPI in a nude mouse/PSN-1 tumor cell xenograft model and in Ki-ras transgenic mice with mammary tumors [8].

Chemical compound and disease context of Nt5c3

• This paper reports procedures for extraction, purification and crystallization of lupanine, and methods for the preparation of an extract for injection of Lupinus mutabilis Sweet, and for the determination of the acute toxicity and maximum non-lethal dose (DL0) of lupanine, sparteine and lupin extract in the mouse [9].

Biological context of Nt5c3

• The survival effect of PNI on motoneurons during the period of programmed cell death was not associated with increased intramuscular nerve branching [5].
• Both p36 phosphorylation and Pk-C specific activity increased as a function of postnatal age [3].
• Thus the extent of p36 phosphorylation was inversely related to the extent of lung cell proliferation in two different physiological states, postnatal growth and regenerative repair following BHT-induced toxic injury [3].
• The DNA sequence of a PN1 type clone revealed its full-length cDNA nature [10].
• In situ hybridization to metaphase chromosome confirmed the somatic cell hybrid data and regionally mapped the C beta gene of PKA to the p36 band on chromosome 1 [11].

Anatomical context of Nt5c3

• In skeletal muscle, expression analysis suggests that Lupin associates with the contractile apparatus [1].
• Lupin is expressed in mouse embryos and adults, notably in liver, spleen, central nervous system, multiple epithelia and all types of muscle [1].
• Deficiency of P5N-1 activity in red blood cells results in nonspherocytic hemolytic anemia [4].
• A human pancreatic cancer cell line, PSN-1, was inoculated into the peritoneal cavity of nude mice [12].
• Mammary gland Ca2+-binding (-dependent) proteins: identification as calelectrins and calpactin I/p36 [13].

Associations of Nt5c3 with chemical compounds

• Such treatment with cedrene prevented any BHT-induced decrease in p36 phosphorylation [3].
• Eukaryotic pyrimidine 5'-nucleotidase type 1 (P5N-1) catalyzes dephosphorylation of pyrimidine 5'-mononucleotides [4].
• In this investigation, we have for the first time used a genetically modified (GM) plant, narrow leaf lupin (Lupinus angustifolius L.), expressing a gene for a potential allergen (sunflower seed albumin) (SSA-lupin) to examine whether a GM plant/food-based vaccine strategy can be used to suppress the development of experimental asthma [14].
• The neurite-promoting activity of PN-1 was inhibited by an anti-PN-1 monoclonal antibody, which blocks its capacity to complex serine proteinases [15].
• Surprisingly, translation of neither protein initiated at the first in-frame methionine, and cells do not express Bag-1/RAP46 proteins with the previously proposed structures; p50 Bag-1 initiates at an upstream CUG codon, whereas p36 Bag-1 initiates at a downstream AUG codon [16].

Regulatory relationships of Nt5c3

• Once daily oral administration of AZD3409 to nude mice bearing PSN-1 and MiaPaCa-2 human pancreatic cancer xenografts expressing mutant K-ras was well tolerated and resulted in a supra-additive reduction in clonogenic cell survival after irradiation [17].

Other interactions of Nt5c3

• Lupin is homologous to a human protein previously called p36, which was purified from alpha-interferon-treated cells that formed lupus inclusions [1].
• The suppression of experimental asthma by SSA-lupin was associated with the production of CD4(+) T cell-derived IFN-gamma and IL-10 [14].
• Furthermore, the inhibition of pyrimidine 5'-nucleotidase (P5N) and delta-aminolevulinic acid dehydratase (ALAD) was studied in the same mice [18].

Analytical, diagnostic and therapeutic context of Nt5c3

• The FTI and the DPI displayed comparable efficacy, causing a decrease in growth rate of the PSN-1 xenograft tumors and tumor regression in the transgenic model, but neither treatment regimen induced a statistically significant increase in tumor cell apoptosis [8].
• The results of an enzyme-linked immunosorbent assay, measuring active, complexed, and truncated PNI, correlated with Western blot data [19].
• PN-1 and ATIII were assayed both by immunohistochemistry and by immunoblots in the brain and spinal cord [20].
• PN-1 mRNA levels were also found to decrease after denervation in the hemidiaphragm but no substantial decrease was observed in denervated hind-limb muscles [21].
• Cytofluorometric analysis of spleen cells revealed that a considerable fraction of all B cells, as well as CD4 and CD8 T lymphocytes, undergo blast transformation after p36 treatment [22].

References