Disease relevance of BMP1

• BMP is found in the cytoplasm of tumor cells of osteosarcoma and chondrosarcoma [1].
• Immunohistochemical demonstration of bone morphogenetic protein in odontogenic tumors [2].
• Immunohistochemical staining showed that BMP plays a role in bone fracture healing [1].
• The ability of BMP-McAb to detect BMP and to inhibit the generation of new bone also makes it potentially useful in diagnosing, treating, and providing a prognosis for osteosarcoma and other bone diseases [1].
• Using this staining method, we can not only differentiate osteosarcoma from fibrosarcoma and other non-bone-derived tumors, but also classify osteosarcoma according to the content and distribution of BMP and the patient's clinical situation, thus providing a scientific basis for clinical treatment [3].

High impact information on BMP1

• Previous studies in our laboratory demonstrated messenger RNA for bone morphogenetic protein-2a in human calcified plaque, suggesting that arterial calcification is a regulated process, similar to osteogenesis [4].
• In addition, cells cultured from the aortic wall formed calcified nodules similar to those found in bone cell cultures and expressed bone morphogenetic protein-2a with prolonged culture [5].
• Purification of bovine bone morphogenetic protein by hydroxyapatite chromatography [6].
• Bovine bone morphogenetic protein (bBMP) induces differentiation of mesenchymal-type cells into cartilage and bone. bBMP has an apparent Mr of 18,500 +/- 500 and represents less than 0.001% of the wet weight of bone tissue [6].
• Cartilage damage and activation of BMP signaling were studied by digital image analysis using Safranin O sulfated glycosaminoglycan staining and immunohistochemistry for phosphorylated Smads (Smads 1, 5, and 8), respectively [7].

Biological context of BMP1

• A fragment containing an exon of the bovine BMP1 gene was amplified from an adapter-ligated genomic DNA library [8].
• The 3' intron region of the amplified genomic BMP1 fragment showed a SSCP polymorphism, and linkage analysis in the International Bovine Reference Panel (IBRP) of families confirmed the boundary position of this gene [8].
• The isolated bovine BMP1 fragment was assigned by fluorescence in situ hybridization (FISH) to bovine (BTA) chromosome 8q21, a region containing an evolutionary breakpoint between homology to human (HSA) chromosomes 8 and 9 [8].
• Oocytes prevent cumulus cell apoptosis by maintaining a morphogenic paracrine gradient of bone morphogenetic proteins [9].
• Regulatory role of transforming growth factor-beta, bone morphogenetic protein-2, and protein-4 on gene expression of extracellular matrix proteins and differentiation of dental pulp cells [10].

Anatomical context of BMP1

• METHODS: Chondrocyte migration was evaluated by 2 assays: 1) "centrifugal" migration within a 3-dimensional collagen matrix (dot culture); and 2) directed migration under agarose in response to bone morphogenetic protein [11].
• Expression of bone morphogenetic protein-6 messenger RNA in bovine growth plate chondrocytes of different size [12].
• Moreover, we show that this assay does not detect a wide range of TGF-beta superfamily members (activin A, bone morphogenetic protein-2, -4, -6, and -7, growth/differentiation factor-5, and glial cell line-derived neurotrophic factor) [13].
• Stimulatory effects of distinct members of the bone morphogenetic protein family on ligament fibroblasts [14].
• Cerebrospinal fluid contains biologically active bone morphogenetic protein-7 [15].

Associations of BMP1 with chemical compounds

• Interaction of osteogenin, a heparin binding bone morphogenetic protein, with type IV collagen [16].
• In contrast, when polylactic acid combined with bovine bone morphogenetic protein had been implanted, significant reparative new bone was not found in the defect at three months [17].
• Guanidine-extracted, demineralized bovine bone, alone or combined with fifteen milligrams of canine bone morphogenetic protein, failed to induce any healing of the non-union [17].
• The quantity of bovine or human BMP/NCP-induced bone formation in rabbits is also increased by very low-dose immunosuppression but not by bone mineral, tricalcium phosphate ceramic, inorganic calcium salts, or various space-occupying, unspecific chemical irritants [18].
• Even after it was extracted with GuHCl, rat bone matrix may have retained trace amounts of endogenous BMP [19].

Regulatory relationships of BMP1

• Bone morphogenetic protein-induced chondrogenesis in outgrowths of muscle connective tissues on various furrowed and unfurrowed fibronectin-coated substrata was observed by biochemical and histologic methods [19].
• Transforming growth factor-beta 2 enhances the osteoinductive activity of a bovine bone-derived fraction containing bone morphogenetic protein-2 and 3 [20].

Other interactions of BMP1

• These results are the first to demonstrate that oocyte-secreted factors, and particularly BMP15 and BMP6, maintain the low incidence of cumulus cell apoptosis by establishing a localized gradient of bone morphogenetic proteins [9].
• OBJECTIVE: To investigate effects of cartilage derived morphogenetic protein-1 and -2 (CDMP-1, CDMP-2), bone morphogenetic protein (BMP)-7 and BMP-6 on metabolism of ligament fibroblasts and their osteogenic or chondrogenic differentiation potential [14].
• The aim of this paper was to elucidate the effects of PTN on bone morphogenetic protein (BMP)-induced ectopic osteogenesis [21].
• The effects of crude bone morphogenetic protein (BMP) derived from bone and dentin matrix on proliferation, production of extracellular matrix, and biologic function of the pulp cell were examined in the primary cell culture from permanent dental pulp [22].

Analytical, diagnostic and therapeutic context of BMP1

• The autografts resulted in the greatest volume of new bone formation (p less than 0.01), but the AA implants elicited a significantly greater response than either the bovine bone morphogenetic protein derivatives or the controls (p less than 0.05) [23].
• Bone morphogenetic protein (BMP) was combined with true bone ceramic (TBC) in an attempt to produce a bone grafting material possessing the cancellous structure, osteoconductivity, and osteoinductivity of biologic bone [24].
• Physical and linkage mapping of the bovine bone morphogenetic protein 1 on the evolutionary break region of BTA 8 [8].
• Preoperative local intramuscular injections of citric, edetic, or hyaluronic acids in specified concentrations markedly enhanced subperiosteal BMP/NCP-induced bone formation [18].
• Bovine bone morphogenetic protein (bBMP) fraction-induced repair of craniotomy defects in the rhesus monkey (Macaca speciosa) [25].

References