Disease relevance of PLS1

• Using transfection experiments, we show the differential recruitment of the two plastin isoforms (T- and L-) into Shigella entry zones [1].
• An invasion-associated Salmonella protein modulates the actin-bundling activity of plastin [2].
• Fibroblasts transformed in vitro by chemical carcinogens or SV40 virus and tumor-derived cancer cells of fibroblastoid or epithelioid origin usually express plastin and p220, a minor phosphorylated form of plastin [3].

High impact information on PLS1

• Moreover, the overexpression of Tc-mip induced Src phosphorylation, T cell clustering, and a cellular redistribution of the cytoskeleton-associated L-plastin, by a PI3 kinase independent pathway [4].
• Since plastin is known to bundle actin filaments in parallel orientation, colocalization of parallel actin filaments and plastin in the cellular protrusions strongly suggested a functional role of this protein in the architecture of parasite-induced cellular projections [1].
• The I-plastin gene was mapped by PCR to human chromosome 3; the L- and T-plastin genes were previously mapped to chromosomes 13 and X, respectively [5].
• I-plastin mRNA was detected in the small intestine, colon, and kidneys; relatively lower levels of expression were detected in the lungs and stomach [5].
• Correction of the N-terminal sequences of the human plastin isoforms by using anchored polymerase chain reaction: identification of a potential calcium-binding domain [6].

Biological context of PLS1

• Human plastin genes. Comparative gene structure, chromosome location, and differential expression in normal and neoplastic cells [7].
• We found that both plastin isoforms contain a potential calcium binding site near the N terminus [6].
• Depletion of intracellular calcium did not affect FcgammaRII-activated L-plastin phosphorylation, demonstrating that any potential regulation of plastin function by calcium did not affect its phosphorylation [8].
• In this report, we describe the genetic linkage mapping of six of these polymorphic proteins (PNIA1-PNIA6), the identification by genetic linkage of a seventh (glyoxalase 1 on 6p21), and support for the mapping of an eighth (plastin or LCP1) to near the ESD locus on Chr 13 [9].
• Three multivariate calibration methods, partial least squares (PLS-1 and PLS-2) and principal component regression (PCR), were applied to the simultaneous determination of three flavour enhancers (inosine 5'-monophosphate, guanosine 5'-monophosphate and monosodium glutamate), in mixtures by ultraviolet/visible absorption spectrophotometry [10].

Anatomical context of PLS1

• Recombinant I-plastin cross-linked actin filaments into bundles in the absence but not in the presence of calcium [5].
• In addition, 4 tumor cell lines that were L-plastin-negative exhibited evidence of defective T-plastin expression increasing the apparent co-incidence of plastin abnormalities associated with human tumorigenesis to 71% [7].
• An oligodeoxynucleotide probe corresponding to one of the oligopeptides was used to clone cDNAs from transformed human fibroblasts that encoded the seven other oligopeptides predicted for human plastin [11].
• The leukocyte isoform l-plastin is expressed in a diverse variety of human tumor cell lines, suggesting that it may be involved in the neoplastic process of some solid human tumors [11].
• Protein microsequencing of plastin purified from leukemic T lymphocytes by high-resolution two-dimensional gel electrophoresis produced eight internal oligopeptide sequences [11].

Associations of PLS1 with chemical compounds

• An array of six potentiometric sensors, constituted by two creatinine biosensors and four ion selective electrodes for potassium, sodium, ammonium and calcium was calibrated and a multivariate model based on PLS1 for the response to creatinine was obtained and validated [12].
• Furthermore, a calibration model using a PLS1 was proposed for the quantification of mannose in the samples obtained by precipitation with 60% ethanol aqueous solutions [13].
• Selected wavelengths in the region 800-2,000 nm were used to make a PLS1 -regression with propionate [14].
• Quantification using a PLS1 regression algorithm showed good correlation with DON reference data, but a rather high standard error of prediction (SEP) with 600 microg/kg (DR) and 490 microg/kg (ATR), respectively, for ergosterol [15].
• Regulation of synthesis of the transformation-induced protein, leukocyte plastin, by ovarian steroid hormones [16].

Regulatory relationships of PLS1

• These data suggest a model in which plastin phosphorylation and localization to the actin cytoskeleton can act as two distinct mechanisms regulating L-plastin functions in neutrophils adherent to immune complexes [8].

Other interactions of PLS1

• Classification models were developed to discriminate between the presence and absence of animal-origin meals in compound feeds using two forms of discriminant partial least squares (PLS) regression: the algorithms PLS1 and PLS2 [17].
• Actin interaction with L-plastin, a plastin/fimbrins isoform of the alpha-actinin family of molecules, is poorly characterized, from the biochemical point of view [18].
• The PLS-1 model was successfully used for determination of epoetin alpha and beta contents in the rHuEPO provided by the EurPh as a biological reference product [19].
• Northern blot analysis revealed that two known sequences, fibrinogen gamma chain and plastin mRNAs were down regulated by TCDD in a time-dependent manner, whereas two unknown mRNAs were induced by TCDD treatment [20].

Analytical, diagnostic and therapeutic context of PLS1

• The complete cDNA sequence of human intestine-specific plastin (I-plastin) was determined from a clone derived by PCR [5].
• Immunofluorescence microscopy detected I-plastin in the brush border of the small intestine and colon [5].
• Molecular cloning and characterization of plastin, a human leukocyte protein expressed in transformed human fibroblasts [11].
• Reliable PLS1 calibration models could be established to correlate the FTIR spectral data and the surface charge of the refined pulps [21].
• Three multivariate calibration methods, partial least squares (PLS-1 and PLS-2) and principal component regression, were applied to the simultaneous determination of the five pesticides iprodione, procymidone, chlorothalonil, folpet and triazophos by high-performance liquid chromatography with diode array detection [22].

References