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Gene Review

PPP5C  -  protein phosphatase 5, catalytic subunit

Homo sapiens

Synonyms: PP-T, PP5, PPP5, PPT, Protein phosphatase T, ...
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Disease relevance of PPP5C


Psychiatry related information on PPP5C

  • These results suggest that PP5 plays a role in the dephosphorylation of tau and might be involved in the molecular pathogenesis of Alzheimer's disease [5].

High impact information on PPP5C


Chemical compound and disease context of PPP5C

  • A dehydrobutyrine (Dhb)-containing microcystin variant [Asp3, ADMAdda5, Dhb7]microcystin-HtyR isolated from Nostoc sp. was found to potently inhibit PP1, PP2A, PPP4 and PPP5 with IC50 values similar to those of microcystin-LR [9].

Biological context of PPP5C


Anatomical context of PPP5C


Associations of PPP5C with chemical compounds

  • Serine/threonine phosphatase 5 (PP5) can act as a suppresser of p53-dependent growth suppression and has been reported to associate with several proteins, including the glucocorticoid receptor/heat-shock protein-90 complex [16].
  • PP5 is a serine/threonine phosphatase that also contains four copies of the tetratricopeptide repeats motif [13].
  • Active forms of Galpha(12) and Galpha(13) also enhance the arachidonic acid-stimulated PP5 phosphatase activity about 2.5-fold [17].
  • Only the NH2-terminal domain of PP5, containing all four tetratricopeptide repeats, is required for this physical interaction [13].
  • Identification of an estrogen-inducible phosphatase (PP5) that converts MCF-7 human breast carcinoma cells into an estrogen-independent phenotype when expressed constitutively [15].

Physical interactions of PPP5C

  • In Rh30 cells, ASK1 was found to physically interact with protein phosphatase 5 (PP5), previously identified as a negative regulator of ASK1 [10].
  • Here we show by a combination of two-hybrid analysis and in vitro binding that PP5 interacts with CDC16 and CDC27, two subunits of the anaphase-promoting complex [13].
  • The TPR domain of PP5 bound specifically to a 12-kDa C-terminal fragment of hsp90 [18].

Regulatory relationships of PPP5C

  • Thus, aberrant PP5 expression may have an additive effect on the development of human cancers by promoting cell proliferation via the inhibition of a GR-induced antiproliferative signaling cascade, and facilitating neoplastic transformation via the inhibition of a growth-arresting p53-mediated response that guards against genomic instability [11].

Other interactions of PPP5C

  • This allowed us to regulate the expression of either p53 (e.g. with ISIS 110332) or PP5 (e.g. with ISIS 15534) in genetically identical cells [16].
  • First, in a rapid response hypoxia facilitates the association of endogenous PP5 with ASK-1 [1].
  • Ser/Thr protein phosphatase type 5 (PP5) is a negative regulator of glucocorticoid receptor-mediated growth arrest [11].
  • In this report, we demonstrate that protein phosphatase 5 (PP5) is required for the ATR-mediated checkpoint activation [7].
  • Employing ISIS 15534, we demonstrate that the specific inhibition of PP5 gene expression has a marked antiproliferative effect on cells, characterized by induction of p21(WAF1/Cip1) and the subsequent arrest of cell growth [19].

Analytical, diagnostic and therapeutic context of PPP5C

  • Conversely, microinjection of PP5 antibodies, or inhibition of its phosphatase activity in vivo, significantly delayed trimer disassembly after heat shock [8].
  • Physical interactions between PP5 and HSF1-Hsp90 complexes were observed in co-immunoprecipitation and gel mobility supershift experiments [8].
  • We have used site-directed mutagenesis to clarify the structural basis for the binding of hsp90 to the TPR domain of phosphoprotein phosphatase 5 (PP5) [18].
  • RESULTS: To further characterize the mechanism by which PP5 affects GR-induced gene expression, we employed immunofluorescence microscopy to track the movement of a GR-green fluorescent fusion protein (GR-GFP) that retained hormone binding, nuclear translocation activity and specific DNA binding activity, but is incapable of transactivation [20].
  • A recombinant adenovirus vector with the PPT sequence, shielded from interfering adenoviral sequences by the mouse H19 insulator, yields high and prostate-specific transgene expression both in cell cultures and when prostate cancer, PC-346C, tumors were grown orthotopically in nude mice [3].


  1. Ser/Thr protein phosphatase 5 inactivates hypoxia-induced activation of an apoptosis signal-regulating kinase 1/MKK-4/JNK signaling cascade. Zhou, G., Golden, T., Aragon, I.V., Honkanen, R.E. J. Biol. Chem. (2004) [Pubmed]
  2. Protein phosphatase 5 is a negative regulator of estrogen receptor-mediated transcription. Ikeda, K., Ogawa, S., Tsukui, T., Horie-Inoue, K., Ouchi, Y., Kato, S., Muramatsu, M., Inoue, S. Mol. Endocrinol. (2004) [Pubmed]
  3. A novel TARP-promoter-based adenovirus against hormone-dependent and hormone-refractory prostate cancer. Cheng, W.S., Kraaij, R., Nilsson, B., van der Weel, L., de Ridder, C.M., Tötterman, T.H., Essand, M. Mol. Ther. (2004) [Pubmed]
  4. Paradoxical Discrepancy Between the Serum Level and the Placental Intensity of PP5/TFPI-2 in Preeclampsia and/or Intrauterine Growth Restriction: Possible Interaction and Correlation with Glypican-3 Hold the Key. Ogawa, M., Yanoma, S., Nagashima, Y., Okamoto, N., Ishikawa, H., Haruki, A., Miyagi, E., Takahashi, T., Hirahara, F., Miyagi, Y. Placenta (2007) [Pubmed]
  5. Dephosphorylation of microtubule-associated protein tau by protein phosphatase 5. Gong, C.X., Liu, F., Wu, G., Rossie, S., Wegiel, J., Li, L., Grundke-Iqbal, I., Iqbal, K. J. Neurochem. (2004) [Pubmed]
  6. Identification of Yin-Yang Regulators and a Phosphorylation Consensus for Male Germ Cell-Associated Kinase (MAK)-Related Kinase. Fu, Z., Larson, K.A., Chitta, R.K., Parker, S.A., Turk, B.E., Lawrence, M.W., Kaldis, P., Galaktionov, K., Cohn, S.M., Shabanowitz, J., Hunt, D.F., Sturgill, T.W. Mol. Cell. Biol. (2006) [Pubmed]
  7. Protein phosphatase 5 is required for ATR-mediated checkpoint activation. Zhang, J., Bao, S., Furumai, R., Kucera, K.S., Ali, A., Dean, N.M., Wang, X.F. Mol. Cell. Biol. (2005) [Pubmed]
  8. Protein phosphatase 5 is a negative modulator of heat shock factor 1. Conde, R., Xavier, J., McLoughlin, C., Chinkers, M., Ovsenek, N. J. Biol. Chem. (2005) [Pubmed]
  9. Inhibition of several protein phosphatases by a non-covalently interacting microcystin and a novel cyanobacterial peptide, nostocyclin. Hastie, C.J., Borthwick, E.B., Morrison, L.F., Codd, G.A., Cohen, P.T. Biochim. Biophys. Acta (2005) [Pubmed]
  10. Inhibition of mammalian target of rapamycin activates apoptosis signal-regulating kinase 1 signaling by suppressing protein phosphatase 5 activity. Huang, S., Shu, L., Easton, J., Harwood, F.C., Germain, G.S., Ichijo, H., Houghton, P.J. J. Biol. Chem. (2004) [Pubmed]
  11. Ser/Thr protein phosphatase type 5 (PP5) is a negative regulator of glucocorticoid receptor-mediated growth arrest. Zuo, Z., Urban, G., Scammell, J.G., Dean, N.M., McLean, T.K., Aragon, I., Honkanen, R.E. Biochemistry (1999) [Pubmed]
  12. Structural basis for the catalytic activity of human serine/threonine protein phosphatase-5. Swingle, M.R., Honkanen, R.E., Ciszak, E.M. J. Biol. Chem. (2004) [Pubmed]
  13. The serine/threonine phosphatase PP5 interacts with CDC16 and CDC27, two tetratricopeptide repeat-containing subunits of the anaphase-promoting complex. Ollendorff, V., Donoghue, D.J. J. Biol. Chem. (1997) [Pubmed]
  14. Inhibition of PP2A, but not PP5, mediates p53 activation by low levels of okadaic acid in rat liver epithelial cells. Messner, D.J., Romeo, C., Boynton, A., Rossie, S. J. Cell. Biochem. (2006) [Pubmed]
  15. Identification of an estrogen-inducible phosphatase (PP5) that converts MCF-7 human breast carcinoma cells into an estrogen-independent phenotype when expressed constitutively. Urban, G., Golden, T., Aragon, I.V., Scammell, J.G., Dean, N.M., Honkanen, R.E. J. Biol. Chem. (2001) [Pubmed]
  16. Identification of a functional link for the p53 tumor suppressor protein in dexamethasone-induced growth suppression. Urban, G., Golden, T., Aragon, I.V., Cowsert, L., Cooper, S.R., Dean, N.M., Honkanen, R.E. J. Biol. Chem. (2003) [Pubmed]
  17. Galpha(12) and Galpha(13) interact with Ser/Thr protein phosphatase type 5 and stimulate its phosphatase activity. Yamaguchi, Y., Katoh, H., Mori, K., Negishi, M. Curr. Biol. (2002) [Pubmed]
  18. Identification of conserved residues required for the binding of a tetratricopeptide repeat domain to heat shock protein 90. Russell, L.C., Whitt, S.R., Chen, M.S., Chinkers, M. J. Biol. Chem. (1999) [Pubmed]
  19. Serine/threonine protein phosphatase type 5 acts upstream of p53 to regulate the induction of p21(WAF1/Cip1) and mediate growth arrest. Zuo, Z., Dean, N.M., Honkanen, R.E. J. Biol. Chem. (1998) [Pubmed]
  20. Serine/threonine protein phosphatase 5 (PP5) participates in the regulation of glucocorticoid receptor nucleocytoplasmic shuttling. Dean, D.A., Urban, G., Aragon, I.V., Swingle, M., Miller, B., Rusconi, S., Bueno, M., Dean, N.M., Honkanen, R.E. BMC Cell Biol. (2001) [Pubmed]
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