Disease relevance of Fast Neutrons

• In this report, we investigated the molecular basis underlying cisplatin resistance in head and neck squamous carcinoma (HNSCC) cells and asked if fast neutron radiation enhances cisplatin cytotoxicity in cisplatin-resistant cells [1].
• Seventy-nine patients with soft tissue sarcoma were treated with fast neutron therapy at the Hammersmith Hospital, MRC Cyclotron Unit [2].
• Combined cyclotron fast-neutron and BCNU therapy in a rat brain-tumor model [3].
• Exponentially growing and plateau phase cultures of Ehrlich ascites tumor cells (suspension strain) were treated with either fast electrons, X-rays, fast neutrons or Am-241-alpha-particles in a dose range from about 0.02 Gy to 1 Gy and for comparison also at higher doses [4].

High impact information on Fast Neutrons

• We have identified 10 additional alleles of rga from a fast-neutron mutagenized ga1-3 population and used them to isolate the RGA gene by genomic subtraction [5].
• A CHI allele, generated by fast-neutron irradiation, consisted of an inversion within the gene [6].
• While direct detection of small mutations, such as an ethyl-methane sulfonate derived single base substitutions, is limited by array coverage and sensitivity, large deletions such as those that can be caused by fast neutrons are easily detected [7].
• The effect of Adriamycin (doxorubicin hydrochloride) (10 mg/kg intraperitoneally) on the response of mouse gut to irradiation with 6-MVp photons or fast neutrons (67 MeV p--Be), was assessed with the six-day death endpoint [8].
• About 60,000 IR64 mutants have been generated by mutagenesis using chemicals (diepoxybutane and ethylmethanesulfonate) and irradiation (fast neutron and gamma ray) [9].

Biological context of Fast Neutrons

• Testis weight loss of C3H and Swiss-Webster (SW) mice was used as endpoint to determine the relative biological effectiveness (RBE) of p(26) + Be fast neutrons with respect to Co-60 gamma irradiation [10].
• Plasmid DNA was irradiated with fast neutrons, and the protection by cysteamine against strand breakage (ssb and dsb) was evaluated in the presence and absence of oxygen [11].
• To identify genes essential for the function of the Xa21 gene, 4,500 IRBB21 ( Xa21 isogenic line in IR24 background) mutants, induced by diepoxybutane and fast neutrons, were screened against Philippine race six (PR6) Xoo for a change from resistance to susceptibility [12].
• The intrachromosomal distribution patterns of chromatid aberrations induced by agents with delayed effects (as exemplified by ethanol and maleic hydrazide) were compared with those produced by agents with non-delayed effects (as exemplified by fast neutrons, X-rays and bleomycin) [13].
• The role of DNA double-strand-breaks in neutron-induced cell death was further studied by comparing the relative sensitivity of the rad52/rad52 mutant to gamma-rays and fast neutrons [14].

Anatomical context of Fast Neutrons

• The relative survival at 1.6 Gy of neutrons (SF1.6) compared to 2 Gy of photons (SF2) (the doses per fractions used in the Clatterbridge fast neutron studies) and the cell kinetic parameters of the 30 cell lines were also compared [15].
• Using fast neutrons produced by impinging protons on a beryllium target at the UCLA/VA cyclotron, we have been examining the energy dependence of mutation induction at the HPRT locus in a human epithelial cell line derived from solid tumor tissue [16].
• These results show that cimetidine can protect bone marrow cells against clastogenic effects of low dose fast neutrons and hence high linear energy transfer (LET) radiation [17].
• Normal 10T1/2 fibroblasts and their transformed counterparts (TCL 15) were used in order to evaluate the RBE, TGF and PLD repair for a therapeutic fast neutron beam (43 MeV proton----Be) [18].
• Effects of split fast neutron doses on the liver cells of albino Swiss mice [19].

Associations of Fast Neutrons with chemical compounds

• Response of the R3327-AT1 Dunning prostate tumor to fast neutrons and cobalt-60 [20].
• METHODS AND MATERIALS: SCC VII tumor-bearing C3H/He mice were irradiated with 30 MeV fast neutrons or 10 MV X rays after receiving 10 injections of 5-bromo-2'-deoxyuridine (BUdR) to label all proliferating tumor cells [21].
• We examined the radioprotective effect of the aminothiol WR-255591 on cultured aerated Chinese hamster ovary cells irradiated with cyclotron-generated fast neutrons [22].
• Optimum fractionation of the C3H mouse mammary carcinoma using x-rays, the hypoxic-cell radiosensitizer Ro-07-0582, or fast neutrons [23].
• Direct effect in DNA radiolysis. Boron neutron capture enhancement of radiolysis in a medical fast-neutron beam [24].

Gene context of Fast Neutrons

• p53 and cell-cycle-regulated protein expression in small intestinal cells after fast-neutron irradiation in mice [25].
• In addition, cyclin B1, cyclin D, and cyclin E were overexpressed in intestinal cells after fast-neutron irradiation and their immunoreactivities were increased strongly in round and oval cells of laminar propria in villi core and crypts [25].
• In order to improve the dose calculation, and thus to use the fast neutron beam for other applications (e.g., Boron Neutron Capture Enhancement of Fast Neutron Therapy), in this work we aim to develop a new TPS [26].
• The induction of potentially lethal damage and potentially mutagenic damage expressed by hypertonic salt treatment in late S-phase V79 cells after exposure to 46-MeV fast neutrons and 50-kVp X rays has been studied [27].
• Proliferation tests and clonogenic assays showed that fast neutrons can nevertheless kill WTK1 and NH32 cells efficiently [28].

Analytical, diagnostic and therapeutic context of Fast Neutrons

• Treatment of stage IIIB cervical cancer with Californium-252 fast-neutron brachytherapy and external photon therapy [29].
• The major goal of MEDICYC, the medical cyclotron programme of the Antoine-Lacassagne Cancer Center (Nice, France) is radiotherapy based on protons and fast neutrons [30].
• Alanine and TLD coupled detectors for fast neutron dose measurements in neutron capture therapy (NCT) [31].
• Twenty eight after whole body irradiation of male BALB/c mice with 0.1-4.0 Gy of 250 kV X-rays or with 0.05-1.0 Gy of 5.5 MeV fast neutrons a decrease in testis weight and number of spermatozoa were observed [32].

References