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Gene Review

Bfsp1  -  beaded filament structural protein 1,...

Rattus norvegicus

Synonyms: 115-kDa, Beaded filament structural protein 1, CP94, CP95, CP97, ...
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Disease relevance of Bfsp1

  • To better understand the role(s) of insulin or its lack and the resultant hyperglycemia in the etiology of these events, peripheral and neuronal (having 125 kDa and 115 kDa alpha subunits, respectively) insulin receptor subtype levels in the retinas of Streptozocin-induced diabetic rats were quantified [1].
  • Isolated perfused mouse hearts subjected to 15 min of ischemia, followed by 30 min of reperfusion, showed complete proteolytic cleavage of 115-kDa NRG-1beta, with concomitant erbB4 phosphorylation [2].
  • Rat insulinoma cells express both a 115-kDa growth hormone receptor and a 95-kDa prolactin receptor structurally related to the hepatic receptors [3].
  • In contrast, the molecular mass of the beta 1-subunit was 130 kDa in Zajdela hepatoma cells versus 115 kDa in normal rat hepatocytes [4].
  • The present study demonstrates that when IgE is bound to the high-affinity receptors, exposure of RBL cells to mycoplasma leads to a time-dependent degradation of the cell-bound IgE into fragments of 186 kDa, 158 kDa and 115 kDa, all of which remain bound to the receptors [5].

High impact information on Bfsp1

  • Using a new mAb raised against the mouse neuroepithelium, we have identified and cDNA-cloned prominin, an 858-amino acid-containing, 115-kDa glycoprotein [6].
  • A third type of receptor could be identified in cerebellar granule cell cultures, where NMDA receptors mediate trophic effects and photolabeling was exclusively targeted to a 115-kDa protein [7].
  • This protein is recognized by an antiserum that detects a protein of 140 kDa and a less abundant species of 115 kDa on NaDodSO4/acrylamide gels [8].
  • The chelating efficiency of CP 20 was comparable with that of deferoxamine (DF), whereas CP 94 was up to eight times more effective than DF [9].
  • A comparative study of the iron-clearing properties of subcutaneously (SC) administered deferoxamine (DFO) with those of orally administered 1,2-dimethyl-3-hydroxypyrid-4-one (CP20) and 1,2-diethyl-3-hydroxypyrid-4-one (CP94) is presented [10].

Chemical compound and disease context of Bfsp1


Biological context of Bfsp1

  • No sequence homologous to the entire CP94 was found among the entries of any nucleotide and amino acid sequence databases; but with respect to a limited amino acid sequence of N-side region of CP94, a significant homology with cytokeratins was found [14].
  • In contrast, T cell activation resulted in only minor up-regulation of 115-kDa CD43 [15].
  • In PC12 cells, the Shc SH2 domain interacts with an unidentified tyrosine-phosphorylated protein of 115 kDa but not with the activated NGF receptor [16].
  • 7. Under conditions favouring the activation of non-NMDA receptors, excitatory synaptic transmission was unaffected by CP94 but was depressed by Zn2+ [17].
  • The areas under the curve (AUCs) for the 25, 50, and 100 mg/kg iv doses were 15, 36, and 72 micrograms/ml/hr, respectively, suggesting that the disposition of CP94 in rats obeys linear kinetics [18].

Anatomical context of Bfsp1

  • Antibodies specifically recognizing the 155- and 115-kDa proteins from hepatocytes inhibited the attachment of these cells to fibronectin-coated dishes, whereas attachment to dishes coated with collagen or laminin was unaffected [19].
  • A counterpart of the 39-kDa hydrophobic component of chromaffin granule H+-ATPase was identified in the membrane, but no 115-kDa component was found [20].
  • On denaturing polyacrylamide gels, the soluble enzyme from bovine pituitary glands appears as two bands of 170 and 135 kDa which are converted to 155 and 115 kDa by endoglycosidase F [21].
  • Specific binding of a novel 40/115-kDa heterodimeric protein complex, which we have named CBP/cycA, to this CCAAT element was detectable in growing but not in G0-arrested or nonadherent normal rat kidney fibroblasts [22].
  • Scatchard analysis of control and collagenase-treated plasma membranes demonstrated that conversion of the 130-kDa subunit to a 115-kDa form did not affect the insulin-binding characteristics of the receptor [23].

Associations of Bfsp1 with chemical compounds

  • Interestingly, removal of sialic acid by neuraminidase treatment of 1B11-negative CD4+ T lymphocytes or 1B11-negative EL4 cells confers 1B11 reactivity, suggesting that the 1B11 epitope is masked by sialic acid residues on the CD43 115-kDa isoform [15].
  • Despite their differing sensitivities to bumetanide and thiazides and their different requirements for potassium, these approximately 115-kDa proteins share significant sequence similarity (approximately 60%) and exhibit a topology featuring 12 potential membrane-spanning helices flanked by long non-hydrophobic domains at the NH2 and COOH termini [24].
  • The LE-1 cDNA sequence was homologous with the 3' portion of the C3 mRNA containing the alpha subunit (115 kDa) [25].
  • In microsomes from DEX-treated rats, DDC analogues caused the formation of higher molecular mass (80, 94, and 115 kDa) proteins showing immunoreactivity with MAb 2-13-1, directed against a major DEX-inducible isozyme belonging to the P-450p family [26].
  • 2. The large depolarizing potentials were reversibly inhibited by the Zn2+ chelator 1,2-diethyl-3-hydroxypyridin-4-one (CP94) [17].

Other interactions of Bfsp1

  • Indicators of lens differentiation included expression of crystallins, lens major intrinsic protein 26 (MIP26), CP49, and filensin and morphologic changes such as cell multilayering and elongation or loss of nuclei [27].
  • The presence of Hrs-2 protein in rat kidney was confirmed by immunoblotting, revealing a 115-kDa protein in kidney and brain membrane fractions corresponding to the expected molecular size of Hrs-2 [28].
  • In the course of characterizing proteins present in a preparation of vesicles from rat adipocytes containing glucose transporters, we examined a protein that migrated at 115 kDa upon SDS gel electrophoresis (designated vp115) [29].
  • At a maximally effective concentration of Endo F, the adipocyte alpha-subunit was reduced from 125-kDa to 100-kDa and the brain alpha-subunit from 115-kDa to 100-kDa [30].
  • CP-94, 253: a selective serotonin1B (5-HT1B) agonist that promotes satiety [31].

Analytical, diagnostic and therapeutic context of Bfsp1


  1. Differential expression of retinal insulin receptors in STZ-induced diabetic rats. Zetterström, C., Benjamin, A., Rosenzweig, S.A. Diabetes (1992) [Pubmed]
  2. Cardiac endothelial cells regulate reactive oxygen species-induced cardiomyocyte apoptosis through neuregulin-1beta/erbB4 signaling. Kuramochi, Y., Cote, G.M., Guo, X., Lebrasseur, N.K., Cui, L., Liao, R., Sawyer, D.B. J. Biol. Chem. (2004) [Pubmed]
  3. Rat insulinoma cells express both a 115-kDa growth hormone receptor and a 95-kDa prolactin receptor structurally related to the hepatic receptors. Møldrup, A., Billestrup, N., Nielsen, J.H. J. Biol. Chem. (1990) [Pubmed]
  4. Biosynthesis, surface expression and function of the fibronectin receptor after rat liver cell transformation to tumorigenicity. Decastel, M., Doyennette-Moyne, M.A., Gouet, E., Aubery, M., Codogno, P. Biochem. J. (1993) [Pubmed]
  5. Mycoplasma-induced degradation of IgE bound by Fc epsilon receptors of rat basophilic leukemia cells. Chan, B.M., Froese, A. Eur. J. Immunol. (1987) [Pubmed]
  6. Prominin, a novel microvilli-specific polytopic membrane protein of the apical surface of epithelial cells, is targeted to plasmalemmal protrusions of non-epithelial cells. Weigmann, A., Corbeil, D., Hellwig, A., Huttner, W.B. Proc. Natl. Acad. Sci. U.S.A. (1997) [Pubmed]
  7. Molecular distinction of three N-methyl-D-aspartate-receptor subtypes in situ and developmental receptor maturation demonstrated with the photoaffinity ligand 125I-labeled CGP 55802A. Marti, T., Benke, D., Mertens, S., Heckendorn, R., Pozza, M., Allgeier, H., Angst, C., Laurie, D., Seeburg, P., Mohler, H. Proc. Natl. Acad. Sci. U.S.A. (1993) [Pubmed]
  8. Characterization of an amyloid beta precursor protein that binds heparin and contains tyrosine sulfate. Schubert, D., LaCorbiere, M., Saitoh, T., Cole, G. Proc. Natl. Acad. Sci. U.S.A. (1989) [Pubmed]
  9. Origin and fate of iron mobilized by the 3-hydroxypyridin-4-one oral chelators: studies in hypertransfused rats by selective radioiron probes of reticuloendothelial and hepatocellular iron stores. Zevin, S., Link, G., Grady, R.W., Hider, R.C., Peter, H.H., Hershko, C. Blood (1992) [Pubmed]
  10. A comparison of the iron-clearing properties of 1,2-dimethyl-3-hydroxypyrid-4-one, 1,2-diethyl-3-hydroxypyrid-4-one, and deferoxamine. Bergeron, R.J., Streiff, R.R., Wiegand, J., Luchetta, G., Creary, E.A., Peter, H.H. Blood (1992) [Pubmed]
  11. Studies of in vivo iron mobilization by chelators in the ferrocene-loaded rat. Florence, A., Ward, R.J., Peters, T.J., Crichton, R.R. Biochem. Pharmacol. (1992) [Pubmed]
  12. Cellular uptake and release of two contrasting iron chelators. Cable, H., Lloyd, J.B. J. Pharm. Pharmacol. (1999) [Pubmed]
  13. Characterization of a new plasma membrane-associated ecto-5'-phosphodiesterase/nucleotide-pyrophosphatase from rat hepatocarcinoma AS-30D cells. García-Nieto, R.M., José, E.S., Martín-Nieto, J., Villalobo, A. Journal of physiology and biochemistry. (2001) [Pubmed]
  14. cDNA sequence analysis of CP94: rat lens fiber cell beaded-filament structural protein shows homology to cytokeratins. Masaki, S., Watanabe, T. Biochem. Biophys. Res. Commun. (1992) [Pubmed]
  15. Characterization of the activation-associated isoform of CD43 on murine T lymphocytes. Jones, A.T., Federsppiel, B., Ellies, L.G., Williams, M.J., Burgener, R., Duronio, V., Smith, C.A., Takei, F., Ziltener, H.J. J. Immunol. (1994) [Pubmed]
  16. Shc binding to nerve growth factor receptor is mediated by the phosphotyrosine interaction domain. Dikic, I., Batzer, A.G., Blaikie, P., Obermeier, A., Ullrich, A., Schlessinger, J., Margolis, B. J. Biol. Chem. (1995) [Pubmed]
  17. Modulation of GABA-mediated synaptic transmission by endogenous zinc in the immature rat hippocampus in vitro. Xie, X., Hider, R.C., Smart, T.G. J. Physiol. (Lond.) (1994) [Pubmed]
  18. The pharmacokinetics of 1,2-diethyl-3-hydroxypyridin-4-one (CP94) in rats. Epemolu, O.R., Singh, S., Hider, R.C., Damani, L.A. Drug Metab. Dispos. (1992) [Pubmed]
  19. Comparison of fibronectin receptors from rat hepatocytes and fibroblasts. Johansson, S., Forsberg, E., Lundgren, B. J. Biol. Chem. (1987) [Pubmed]
  20. H+-translocating ATPase in Golgi apparatus. Characterization as vacuolar H+-ATPase and its subunit structures. Moriyama, Y., Nelson, N. J. Biol. Chem. (1989) [Pubmed]
  21. Tissue distribution and characterization of soluble and membrane-bound forms of metallocarboxypeptidase D. Song, L., Fricker, L.D. J. Biol. Chem. (1996) [Pubmed]
  22. A novel CCAAT-binding protein necessary for adhesion-dependent cyclin A transcription at the G1/S boundary is sequestered by a retinoblastoma-like protein in G0. Krämer, A., Carstens, C.P., Fahl, W.E. J. Biol. Chem. (1996) [Pubmed]
  23. Photoaffinity labelling of hepatic plasma membranes suggests two classes of hepatic insulin receptor. Haynes, F.J., Yip, C.C. Diabetologia (1985) [Pubmed]
  24. Molecular cloning, primary structure, and characterization of two members of the mammalian electroneutral sodium-(potassium)-chloride cotransporter family expressed in kidney. Gamba, G., Miyanoshita, A., Lombardi, M., Lytton, J., Lee, W.S., Hediger, M.A., Hebert, S.C. J. Biol. Chem. (1994) [Pubmed]
  25. Estrogen regulation of tissue-specific expression of complement C3. Sundstrom, S.A., Komm, B.S., Ponce-de-Leon, H., Yi, Z., Teuscher, C., Lyttle, C.R. J. Biol. Chem. (1989) [Pubmed]
  26. Effects of a series of 4-alkyl analogues of 3,5-diethoxycarbonyl-1,4-dihydro-2,4,6-trimethylpyridine on the major inducible cytochrome P-450 isozymes of rat liver. Riddick, D.S., Park, S.S., Gelboin, H.V., Marks, G.S. Mol. Pharmacol. (1989) [Pubmed]
  27. Ubiquitin-proteasome pathway function is required for lens cell proliferation and differentiation. Guo, W., Shang, F., Liu, Q., Urim, L., Zhang, M., Taylor, A. Invest. Ophthalmol. Vis. Sci. (2006) [Pubmed]
  28. SNAP-25-associated Hrs-2 protein colocalizes with AQP2 in rat kidney collecting duct principal cells. Shukla, A., Hager, H., Corydon, T.J., Bean, A.J., Dahl, R., Vajda, Z., Li, H., Hoffmann, H.J., Nielsen, S. Am. J. Physiol. Renal Physiol. (2001) [Pubmed]
  29. Cloning and preliminary characterization of a 121 kDa protein with multiple predicted C2 domains. Morris, N.J., Ross, S.A., Neveu, J.M., Lane, W.S., Lienhard, G.E. Biochim. Biophys. Acta (1999) [Pubmed]
  30. Oligosaccharide heterogeneity of insulin receptors. Comparison of N-linked glycosylation of insulin receptors in adipocytes and brain. Heidenreich, K.A., Brandenburg, D. Endocrinology (1986) [Pubmed]
  31. CP-94, 253: a selective serotonin1B (5-HT1B) agonist that promotes satiety. Lee, M.D., Simansky, K.J. Psychopharmacology (Berl.) (1997) [Pubmed]
  32. Transfected rat high-molecular-weight neurofilament (NF-H) coassembles with vimentin in a predominantly nonphosphorylated form. Chin, S.S., Liem, R.K. J. Neurosci. (1990) [Pubmed]
  33. Rat sperm plasma membrane mannosidase: localization and evidence for proteolytic processing during epididymal maturation. Tulsiani, D.R., NagDas, S.K., Skudlarek, M.D., Orgebin-Crist, M.C. Dev. Biol. (1995) [Pubmed]
  34. High-performance liquid chromatographic determination of 1,2-diethyl-3-hydroxypyridin-4-one and its 2-(1-hydroxyethyl) metabolite in rat blood. Epemolu, R.O., Singh, S., Hider, R.C., Damani, L.A. J. Chromatogr. (1992) [Pubmed]
  35. Contrasting interspecies efficacy and toxicology of 1,2-diethyl-3-hydroxypyridin-4-one, CP94, relates to differing metabolism of the iron chelating site. Porter, J.B., Abeysinghe, R.D., Hoyes, K.P., Barra, C., Huehns, E.R., Brooks, P.N., Blackwell, M.P., Araneta, M., Brittenham, G., Singh, S. Br. J. Haematol. (1993) [Pubmed]
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