Disease relevance of TCEA1

• In this work, the rates for equilibration between the active and pausing pathways were estimated in the absence of an elongation factor, in the presence of hepatitis delta antigen (HDAg), and in the presence of transcription factor IIF (TFIIF), with or without addition of SII [1].
• The cDNA for the human elongation factor, TFIIS, has been cloned and expressed in E. coli with the T7 expression system [2].
• Previously it was shown that human foamy virus (HFV) RNA transcribed in vitro contained three sites, designated SI, SII, and SIII, which contributed to the dimerization process (O. Erlwein, D. Cain, N. Fischer, A. Rethwilm, and M. O. McClure, Virology 229:251-258, 1997) [3].
• The survival of patients with initially removed SI and SII metastases is much better, similar to that of patients with S0 metastases [4].
• Labeling of Ehrlich ascites cells with radioactive uridine and a subsequent chase in the presence of RNA-synthesis inhibitors shows that radioactivity was incorporated first in precursor particles with a density of 1.48 g/cm3 and then subsequently appeared in SIII and SII particles [5].

Psychiatry related information on TCEA1

• This study implies that SII activation is modulated by motor activity and that the effect depends on the topographical proximity of the stimulated and contracted body parts [6].
• In contrast, SII activity showed an S-shaped function with a sharp increase in amplitude only at a stimulus intensity well above pain threshold [7].
• Left- and right-sided intervening stimuli affected similarly the SII responses and had no effect on the response latencies [8].
• Lesions in the SII cortex may disturb the self-perception of body scheme [9].
• RESULTS: From the analysis of the psychometric properties of the three instruments, the SSI/SII appeared to have the best psychometric profile; Grade A, BFLUTS was of grade A, and KHQ was of grade B [10].

High impact information on TCEA1

• The transcription elongation factor SII is a major component of CTEA and strongly synergizes with p300 (histone acetylation) at a step subsequent to preinitiation complex formation [11].
• The transcription elongation factor TFIIS induces mRNA cleavage by enhancing the intrinsic nuclease activity of RNA polymerase (Pol) II [12].
• CRSPs 34, 77 and 130 are new proteins, but the amino terminus of CRSP70 is homologous to elongation factor TFIIS [13].
• The representations of the visual field in SI and SII showed a partially retinotopic organization [14].
• We used simultaneous multi-site neural ensemble recordings to investigate the representation of tactile information in three areas of the primate somatosensory cortex (areas 3b, SII and 2) [15].

Chemical compound and disease context of TCEA1

• RESULTS--During the study 49 patients developed 21 episodes of bacteraemia due to the outbreak strain, which was ciprofloxacin resistant (minimum inhibitory concentration greater than or equal to 128 mg/l), susceptible to phage 155 A9C, and SII biotype and had characteristic immunoblot and DNA fingerprint features [16].

Biological context of TCEA1

• These regions are similar in amino acid sequence but dispensable for TFIIS or Elongin to regulate elongation in vitro by highly purified RNA polymerase II [17].
• Using PCR primers, verified by RT-PCR to amplify the authentic, transcriptionally active SII gene, this locus was mapped to human chromosome 3 by examination of a human/rodent somatic cell hybrid panel [18].
• A 1.2-kb cDNA clone isolated from a HeLa cell library contains an open reading frame encoding a new protein (p21) of 21 kDa that is approx. 48% similar to members of the eukaryotic transcription elongation factor SII (TFIIS) family [19].
• This shows that the 3'-end of RNA is near the SII binding site on RNA polymerase II and suggests that SII may activate the intrinsic RNA hydrolysis activity by positioning the transcript in the enzyme's active site [20].
• Elongation factor SII (also known as TFIIS) is an RNA polymerase II binding protein that allows bypass of template arrest sites by activating a nascent RNA cleavage reaction [20].

Anatomical context of TCEA1

• A homogeneous preparation of calf thymus SII can also provide this activity in trans [21].
• Phosphorylation of HeLa SII (or TFIIS)-related nuclear protein p21/SIIR was demonstrated in transfected COS-1 cells [22].
• Five somatosensory cortices have distinctive somatotopic representations, cytoarchitecture, and connectivity: primary somatosensory cortex (SI), ventrolateral association cortices (SII, SIII, and SIV), and dorsomedial association cortex (supplementary sensory area) [23].
• Also in contrast to the previously reported TFIIS gene, the testis gene contains introns [24].
• We aimed at characterizing phase locking between SI and SII in response to electric stimuli applied once every 3 s to the right median nerve at the wrist; phase locking between brain regions has been proposed to either reflect joined processing or information exchange [25].

Associations of TCEA1 with chemical compounds

• The elongation factors Elongin and CSB, but not TFIIS, can also stimulate bypass of thymine glycol lesions, whereas Elongin, CSB and TFIIS can all enhance bypass of an 8-oxoguanine lesion [26].
• Since the elongation complexes were released by sarkosyl but not by SII, these complexes apparently did not enter the arrested conformation when they encountered nucleosomes [27].
• Furthermore, RNAPII complexes arrested at dL were subject to the transcript cleavage reaction mediated by elongation factor TFIIS, indicating that these complexes were stable [28].
• Cation positions are displaced only slightly by interaction with methanol, although somewhat more at the SIII sites than the SII [29].
• The adsorption energy of N(2) and O(2) is the highest for Li(+) cations at the SIII' cation sites, while for the SI' and SII sites the adsorption energies decrease in the order Ca(2+) > Na(+) > Li(+) [30].

Other interactions of TCEA1

• By using an in vitro binding assay, we show that EloA-BP1 is capable of binding not only the NH(2)-terminal approximately 120 amino acid region of Elongin A, but also that of SII [31].
• Interaction of elongation factors TFIIS and elongin A with a human RNA polymerase II holoenzyme capable of promoter-specific initiation and responsive to transcriptional activators [17].
• Genomic characterization of a testis-specific TFIIS (TCEA2) gene [24].
• Structure of a conserved domain common to the transcription factors TFIIS, elongin A, and CRSP70 [32].
• In vitro binding studies showed that SI and SIII, but not SII, interact with nuclear proteins from DBH-negative cells in a cell-specific manner [33].

Analytical, diagnostic and therapeutic context of TCEA1

• Affinity chromatography on columns containing the immobilized monomeric transcriptional elongation factor TFIIS or the essential large subunit, Elongin A, of the trimeric elongation factor, Elongin, was used to purify a human RNA polymerase II holoenzyme from HeLa whole cell extract [17].
• In EEG recordings (evoked potentials), no potential corresponding to N100m-P100m was found, probably because it was difficult to record activation in SII by EEG recordings [34].
• Visual stimulation within well-defined receptive fields reliably evoked multi-unit responses in SI and SII of operated, but not normal hamsters [14].
• In addition, Western blot analysis using SII-specific antibodies revealed that human SII is a major component in purified RTF preparations [35].
• We have determined, using gel filtration, that the intron 1 arrest site is a stable RNA polymerase II pause site and that the transcription elongation factor SII promotes read-through at this site [36].

References