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Gene Review

VAMP3  -  vesicle-associated membrane protein 3

Homo sapiens

Synonyms: CEB, Cellubrevin, SYB3, Synaptobrevin-3, VAMP-3, ...
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Disease relevance of VAMP3

  • Unlike the effect of insulin, the GLUT4myc gain caused by hyperosmolarity was insensitive to wortmannin or to tetanus toxin cleavage of VAMP2 and VAMP3 [1].
  • Hyperacidification of cellubrevin endocytic compartments and defective endosomal recycling in cystic fibrosis respiratory epithelial cells [2].
  • BACKGROUND: Here we evaluate auto-antibody response against two potential antigenic determinants of genes highly expressed in low Gleason Score prostate cancer (PC) tumor samples, namely FLJ23438 and VAMP3 [3].
  • The auto-antibody response against FLJ23438 and VAMP3 recombinant proteins was tested by immunoblot assays using PC, benign prostate hyperplasia (BPH), healthy donors (HD), and other human cancers plasma samples [3].
  • The toxicity of CEB included bone marrow suppression and alopecia in all patients but no significant neurotoxicity or ototoxicity, and minimal renal toxicity [4].

High impact information on VAMP3

  • Insulin-induced ATP release from human platelets correlated with the association of syntaxin 2 with the vesicle-associated membrane protein 3 but was not associated with the activation of alphaIIbbeta3 integrin [5].
  • Using green fluorescent protein (GFP) as both a fluorescent indicator and an exofacial epitope tag, we show that polarized fusion of VAMP3 vesicles precedes phagosome sealing [6].
  • In cells overexpressing Rab11wt and in a GTPase-deficient Rab11 mutant (Rab11Q70L), these proteins were found in vesicles showing characteristics of sorting endosomes lacking cellubrevin (Cb) [7].
  • Furthermore, we have shown that the ARF6-containing intracellular compartment partially colocalized with transferrin receptors and cellubrevin and morphologically resembled the recycling endocytic compartment previously described in CHO cells [8].
  • Export of cellubrevin from the endoplasmic reticulum is controlled by BAP31 [9].

Chemical compound and disease context of VAMP3


Biological context of VAMP3


Anatomical context of VAMP3


Associations of VAMP3 with chemical compounds


Other interactions of VAMP3


Analytical, diagnostic and therapeutic context of VAMP3

  • METHODS: RT-PCR assays were used to analyze mRNA expression profiles of FLJ23438 and VAMP3 transcripts [3].
  • Western blotting of wild-type, heterozygous, and null platelets confirmed the lack of cellubrevin/VAMP-3 in nulls and showed that most elements of the secretion machinery are expressed at similar levels [25].
  • When BHK cells were analyzed by immunocytochemistry, BAP31 did not overlap with cellubrevin, but rather colocalized with resident proteins of the ER [9].
  • Five patients failed CEB chemotherapy, but all have been successfully salvaged with a combination of surgery and intensive chemotherapy, follow-up from completion of all treatment being 35 to 44 months [4].
  • The CVP/CEB overall survival and event-free survival rates are 55% and 32%, respectively, and they are not statistically different from those of patients treated before 1990 [26].


  1. Hyperosmolarity reduces GLUT4 endocytosis and increases its exocytosis from a VAMP2-independent pool in l6 muscle cells. Li, D., Randhawa, V.K., Patel, N., Hayashi, M., Klip, A. J. Biol. Chem. (2001) [Pubmed]
  2. Hyperacidification of cellubrevin endocytic compartments and defective endosomal recycling in cystic fibrosis respiratory epithelial cells. Poschet, J.F., Skidmore, J., Boucher, J.C., Firoved, A.M., Van Dyke, R.W., Deretic, V. J. Biol. Chem. (2002) [Pubmed]
  3. Auto-antibodies in prostate cancer: Humoral immune response to antigenic determinants coded by the differentially expressed transcripts FLJ23438 and VAMP3. Pontes, E.R., Matos, L.C., da Silva, E.A., Xavier, L.S., Diaz, B.L., Small, I.A., Reis, E.M., Verjovski-Almeida, S., Barcinski, M.A., Gimba, E.R. Prostate (2006) [Pubmed]
  4. Effectiveness of carboplatin, etoposide, and bleomycin combination chemotherapy in good-prognosis metastatic testicular nonseminomatous germ cell tumors. Horwich, A., Dearnaley, D.P., Nicholls, J., Jay, G., Mason, M., Harland, S., Peckham, M.J., Hendry, W.F. J. Clin. Oncol. (1991) [Pubmed]
  5. Insulin induces the release of vasodilator compounds from platelets by a nitric oxide-G kinase-VAMP-3-dependent pathway. Randriamboavonjy, V., Schrader, J., Busse, R., Fleming, I. J. Exp. Med. (2004) [Pubmed]
  6. Focal exocytosis of VAMP3-containing vesicles at sites of phagosome formation. Bajno, L., Peng, X.R., Schreiber, A.D., Moore, H.P., Trimble, W.S., Grinstein, S. J. Cell Biol. (2000) [Pubmed]
  7. Rab11 regulates the compartmentalization of early endosomes required for efficient transport from early endosomes to the trans-golgi network. Wilcke, M., Johannes, L., Galli, T., Mayau, V., Goud, B., Salamero, J. J. Cell Biol. (2000) [Pubmed]
  8. ARF6 targets recycling vesicles to the plasma membrane: insights from an ultrastructural investigation. D'Souza-Schorey, C., van Donselaar, E., Hsu, V.W., Yang, C., Stahl, P.D., Peters, P.J. J. Cell Biol. (1998) [Pubmed]
  9. Export of cellubrevin from the endoplasmic reticulum is controlled by BAP31. Annaert, W.G., Becker, B., Kistner, U., Reth, M., Jahn, R. J. Cell Biol. (1997) [Pubmed]
  10. A randomized trial of cisplatin, etoposide and bleomycin (PEB) versus carboplatin, etoposide and bleomycin (CEB) for patients with 'good-risk' metastatic non-seminomatous germ cell tumors. Bokemeyer, C., Köhrmann, O., Tischler, J., Weissbach, L., Räth, U., Haupt, A., Schöffski, P., Harstrick, A., Schmoll, H.J. Ann. Oncol. (1996) [Pubmed]
  11. High-dose CEB vs BEAM with autologous stem cell transplant in lymphoma. Wang, E.H., Chen, Y.A., Corringham, S., Bashey, A., Holman, P., Ball, E.D., Carrier, E. Bone Marrow Transplant. (2004) [Pubmed]
  12. Inhibition of SNARE-mediated membrane traffic impairs cell migration. Tayeb, M.A., Skalski, M., Cha, M.C., Kean, M.J., Scaife, M., Coppolino, M.G. Exp. Cell Res. (2005) [Pubmed]
  13. Tetanus toxin-mediated cleavage of cellubrevin impairs exocytosis of transferrin receptor-containing vesicles in CHO cells. Galli, T., Chilcote, T., Mundigl, O., Binz, T., Niemann, H., De Camilli, P. J. Cell Biol. (1994) [Pubmed]
  14. SNARE protein degradation upon platelet activation: calpain cleaves SNAP-23. Lai, K.C., Flaumenhaft, R. J. Cell. Physiol. (2003) [Pubmed]
  15. Rabaptin4, a novel effector of the small GTPase rab4a, is recruited to perinuclear recycling vesicles. Nagelkerken, B., Van Anken, E., Van Raak, M., Gerez, L., Mohrmann, K., Van Uden, N., Holthuizen, J., Pelkmans, L., Van Der Sluijs, P. Biochem. J. (2000) [Pubmed]
  16. Cytokine Secretion via Cholesterol-rich Lipid Raft-associated SNAREs at the Phagocytic Cup. Kay, J.G., Murray, R.Z., Pagan, J.K., Stow, J.L. J. Biol. Chem. (2006) [Pubmed]
  17. Localization of cellubrevin-related peptide, endobrevin, in the early endosome in pancreatic beta cells and its physiological function in exo-endocytosis of secretory granules. Nagamatsu, S., Nakamichi, Y., Watanabe, T., Matsushima, S., Yamaguchi, S., Ni, J., Itagaki, E., Ishida, H. J. Cell. Sci. (2001) [Pubmed]
  18. Ectopic expression of syntaxin 1 in the ER redirects TI-VAMP- and cellubrevin-containing vesicles. Martinez-Arca, S., Proux-Gillardeaux, V., Alberts, P., Louvard, D., Galli, T. J. Cell. Sci. (2003) [Pubmed]
  19. Mutational analysis of VAMP domains implicated in Ca2+-induced insulin exocytosis. Regazzi, R., Sadoul, K., Meda, P., Kelly, R.B., Halban, P.A., Wollheim, C.B. EMBO J. (1996) [Pubmed]
  20. Cellubrevin-targeted fluorescence uncovers heterogeneity in the recycling endosomes. Teter, K., Chandy, G., Quiñones, B., Pereyra, K., Machen, T., Moore, H.P. J. Biol. Chem. (1998) [Pubmed]
  21. Sexual dysfunction in nonseminoma testicular cancer patients is related to chemotherapy-induced angiopathy. van Basten, J.P., Hoekstra, H.J., van Driel, M.F., Koops, H.S., Droste, J.H., Jonker-Pool, G., van de Wiel, H.B., Sleijfer, D.T. J. Clin. Oncol. (1997) [Pubmed]
  22. Effects of calcium channel blockers on renal function. Chan, L., Schrier, R.W. Annu. Rev. Med. (1990) [Pubmed]
  23. Vesicle-associated membrane protein 3 (VAMP-3) and VAMP-8 are present in human platelets and are required for granule secretion. Polgár, J., Chung, S.H., Reed, G.L. Blood (2002) [Pubmed]
  24. A role for Sec1/Munc18 proteins in platelet exocytosis. Schraw, T.D., Lemons, P.P., Dean, W.L., Whiteheart, S.W. Biochem. J. (2003) [Pubmed]
  25. Granule stores from cellubrevin/VAMP-3 null mouse platelets exhibit normal stimulus-induced release. Schraw, T.D., Rutledge, T.W., Crawford, G.L., Bernstein, A.M., Kalen, A.L., Pessin, J.E., Whiteheart, S.W. Blood (2003) [Pubmed]
  26. Results of a low aggressivity chemotherapy regimen (CVP/CEB) in elderly Hodgkin's disease patients. Levis, A., Depaoli, L., Bertini, M., Botto, B., Ciravegna, G., Freilone, R., Gallamini, A., Gavarotti, P., Ricardi, U., Scalabrini, D.R., Salomone, A., Salvi, F., Vitolo, U., Pileri, A., Sannazzari, G.L., Resegotti, L. Haematologica (1996) [Pubmed]
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