Disease relevance of Slc7a2

• CAT-2 expression was observed in hepatocytes in vitro and in vivo, consistent with reports of infection of regenerating and cultured hepatocytes by amphotropic retroviruses [1].
• Marked eosinophilia, associated with up-regulation of eotaxin-1, was present in the bronchoalveolar lavage fluid of 3-week-old CAT2-deficient mice [2].
• The prevention of lung cancer induced by a tobacco-specific carcinogen in rodents by green and black Tea [3].
• Oxcarbazepine (OCBZ, Trileptal) and its main human monohydroxy metabolite (MHD) protected mice and rats against generalized tonic-clonic seizures induced by electroshock with ED50 values between 13.5 and 20.5 mg/kg p.o. No tolerance toward this anticonvulsant effect was observed when rats were treated with OCBZ or MHD daily for 4 weeks [4].
• Similarly, the Ca(2+)-regulated K(+) channel blockers, BaCl(2) and quinidine, decreased the K(+) current by 20.5 +/- 1.0% and 36.6 +/- 1.2%, respectively, and slowed migration of B-16 melanoma cells by 37.1 +/- 8.6% and 42.7 +/- 8.8%, respectively [5].

Psychiatry related information on Slc7a2

• Unexpectedly, CAT2a transcripts accumulated in skeletal muscle in response to surgical trauma (hepatectomy and splenectomy) as well as food deprivation, although neither high affinity CAT2 nor CAT1 were detectably altered [6].
• We observed high-grade individual differences in sleep-time that ranged from 0 to 179 min, 83.1 +/- 4.3 (mean and SEM of 100 mice) for ethanol; 0 to 169 min, 64.5 +/- 3.1 for pentobarbital; 0 to 160 min, 36.6 +/- 3.6 for urethane; 0 to 120 min, 21.5 +/- 2.2 for tribromoethanol; and 3 to 20.5 min, 7.1 +/- 0.3 for propofol [7].

High impact information on Slc7a2

• Thus, our data suggest that CAT2 regulates anti-inflammatory processes in the lungs via regulation of dendritic cell activation and subsequent T cell responses [2].
• Despite the presence of activated alveolar macrophages in CAT2-deficient mice, NO production was compromised in these cells [2].
• The Tea gene is distinct from, but bears extensive amino acid and DNA sequence similarity with, the murine ecotropic retroviral receptor which is encoded by the Rec-1 gene [8].
• The gene was expressed on concanavalin A-activated T lymphocytes and was designated Tea (T-cell early activation gene) [8].
• The Tea gene mapped to chromosome 8 and appeared to be conserved among mammalian and avian species [8].

Chemical compound and disease context of Slc7a2

• Effect of Ascorbic Acid, Lysine, Proline, and Green Tea Extract on Human Osteosarcoma Cell Line MNNG-HOS Xenografts in Nude Mice: Evaluation of Tumor Growth and Immunohistochemistry [9].

Biological context of Slc7a2

• Although the amino acid sequences of the isoforms differ in only 21 of 43 residues with the majority of substitutions being conservative, the apparent affinity of CAT2 beta for arginine uptake was 70-fold higher than the CAT2 alpha isoform (Km 38 microM versus 2.7 mM) [10].
• No change in promoter usage was apparent under any stress conditions tested nor was alternate splicing of the CAT2 transcript dictated by promoter usage [6].
• IL-10 also attenuated the LPS-induced upregulation of CAT-2 mRNA [11].
• In contrast, mRNA for the cationic amino acid transporter, CAT2, was induced by plasmid DNA alone, and priming with IFN-gamma had no effect on this response [12].
• After subtraction of the zero-time values, the initial rates of influx for VM-26 (extracellular concentration, 20.5 microM) and the apparent equilibrium constants for the flux of drug across the cell membrane correlated inversely with the logarithm of the IC50 values [13].

Anatomical context of Slc7a2

• The expression of CAT2/2a transcripts was tested in skeletal muscle and macrophages following specific stresses or activators [6].
• In macrophages, liver and skeletal muscle, the most distal CAT2 promoter was predominant [6].
• Here we report that iNOS activity is impaired by 84% in activated Cat2-deficient astrocytes [14].
• We report an extended and corrected Tea cDNA sequence and show that the mammalian Tea and ERR encoding genes are differentially expressed in tissues and cell lines [15].
• When the dorsal skins of 8.5-, 20.5-, and 60.5-day-old mice were wounded, the increase in the melanocyte and melanoblast-melanocyte populations was much smaller than the newborn mice [16].

Associations of Slc7a2 with chemical compounds

• The effects of lidocaine on CAT-2 and GTPCH are unknown [17].
• Chimeric mice with TNF-deficient leukocytes but intact intrinsic renal cell-derived TNF (crescents, 20.5 +/- 2.0% of glomeruli; serum creatinine, 21.6 +/- 1.4 micromol/L) developed similar crescentic GN to WT mice (crescents, 22.3 +/- 1.4% of glomeruli; serum creatinine, 24.8 +/- 1.9 micromol/L) [18].
• An increase in malondialdehyde content (nmol microg of protein(-1)), especially at high CdCl(2) concentrations (control = 7.3 +/- 0.5; CdCl(2): 7.5 microM = 11.2 +/- 3.1, 10 microM = 14.6 +/- 3.8, and 12.5 microM = 20.5 +/- 6.5) indicated that there was enhanced lipid peroxidation [19].
• In these neuronal hybrids, we have found that bradykinin induces sequential elevation in the concentrations of several second messengers involved in neuronal activation, including inositol trisphosphate (6.5-fold), intracellular calcium (2.7-fold), and cyclic GMP (20.5-fold) [20].
• Isop significantly increased androgen production by 20.5-day rat and 17.5-day mouse fetuses after a time lag of 30 and 180 min, respectively [21].

Other interactions of Slc7a2

• The increase in arginine transport during activation, but not proliferation, was mediated by the SLC7A2/Cat2 gene [22].
• Our results support the idea that manipulation of CAT2 transporter function might be useful for the therapeutic modulation of iNOS activity [14].
• These results suggest that NO is produced in the mouse lung in response to F. kyushuense exposure and that the NO production is regulated by CAT2, the citrulline-NO cycle, and arginase isoforms [23].
• Recently, it has been suggested that CAT-2 may be a receptor for amphotropic murine leukemia virus [1].

Analytical, diagnostic and therapeutic context of Slc7a2

• In situ hybridization on brain sections revealed that CAT3 transcripts were localized predominantly along the midbrain-thalamus-hypothalamus axis, whereas neither CAT1 nor CAT2 transcripts demonstrated a similar localization [24].
• In addition, mRNA encoding the cationic amino acid transporter-2 (CAT-2) was strongly induced shortly after treatment [25].
• Immunoprecipitation and SDS-PAGE analysis identified 20.5- and 18-kDa proteins with mAb H1-229 or H2-439, respectively, in cellular extracts of 125I-surface labeled NS-1 transfected with the corresponding genes [26].
• Western blot analysis, using an anti-rodent bFGF antibody, shows multiple molecular weights of 18, 20.5, and 22 kDa of bFGF protein isolated from the adult retina [27].
• Analysis of these conjugates by high performance liquid chromatography revealed a major peak eluting at 20.5 min [28].

References