Disease relevance of CRISP1

• ARP exacerbations were associated with significantly higher plasma levels of the inflammatory marker soluble intracellular adhesion molecule 1 than non-ARP exacerbations, indicating relatively enhanced immune activation during ARP relapses [1].
• The absolute (ARP) and relative refractory period (RRP) of the median sensory nerve was determined in 26 control subjects and 24 alcoholics, nine of whom had symptoms of peripheral neuropathy [2].
• Our studies on the relationships among the lymphoid system, apoptosis and apoptosis-related proteins (ARP) in human ovarian benign cysts, borderline tumors, and carcinomas are reviewed and analyzed [3].
• CONCLUSION: Plasma levels of AVP, Aldo, and ARP increase during exercise when a threshold is reached and thereafter are dependent on the absolute workload, without any specific effect of hypoxia [4].
• Human APOBEC3G (hA3G) is a member of the APOBEC-1 related protein (ARP) family of cytidine deaminases. hA3G functions as a natural defense against endogenous retrotransposons and a multitude of retroviruses, most notably human immunodeficiency virus type 1 (HIV-1) [5].

Psychiatry related information on CRISP1

• Biological and developmental vulnerabilities, maladaptive learning experiences, and pathological family patterns may all contribute to the appearance and persistence of eating disorder (Bruch 1973, Crisp 1980, Garfinkel and Garner 1982) [6].
• Scores on the Crown Crisp Experimental Index indicate a significant difference in the profiles of somatization and hysteria [7].
• The subtleties of diagnosing anorexia nervosa have recently been recognized in this group (e.g. Cottrell & Crisp, 1984) [8].
• Psychological theories of aetiology are discussed with reference to Bruch, Crisp, Palazzoli and Minuchin: the common theme is the reaction of the patient and her family to the physical and social changes of puberty [9].
• Initial interest in the relationship between eating disorders, which occur primarily in women, and substance abuse, which is much more frequent in men than women, stemmed from the observations of Crisp (1968) who noted that chronic anorexics who developed bulimic behavior often abused alcohol [10].

High impact information on CRISP1

• We amplified and sequenced the multiple arginine coding area of the ARP gene in primary head and neck, non-small cell lung, and renal cell cancers [11].
• The arginine-rich protein (ARP) gene was recently cloned and localized to human chromosome band 3p21 [11].
• Thus, the variations in the ARP trinucleotide repeat region represent normal polymorphisms rather than tumor-specific mutations [11].
• Physiologically relevant ARP26)levels promoted AChE gene expression and induced the expansion of cultured CD34+ progenitors and granulocyte maturation more effectively than cortisol, suggesting autoregulatory prolongation of ARP effects [12].
• A high-quality multiple alignment of approximately 700 complete protein sequences homologous to actin, including 148 ARP sequences, allowed us to extend the ARP classification to new organisms [13].

Biological context of CRISP1

• Mammalian members of the cysteine-rich secretory protein (CRISP) family are expressed predominantly in the male reproductive tract and are implicated in the process of reproduction from spermiogenesis, posttesticular sperm maturation, and capacitation to oocyte-sperm fusion, and possibly also penetration of the zona pellucida [14].
• 2. This result indicates that AEGL1 and the mouse gene for AEG are located in the chromosomal segments with conserved syntenies [15].
• The amino acid sequence predicted from the complete nucleotide sequence of this clone is remarkable because 40% of the residues are Asn, and so the antigen has been termed the Asparagine-Rich Protein (ARP) [16].
• The interesting finding that the binding site of DE resides in an evolutionarily conserved region of the molecule provides novel information on the molecular mechanisms underlying CRISP-1 function in gamete fusion with important implications on the structure-function relationship of other members of the widely distributed CRISP family [17].
• Kinetic studies of ARP phosphorylation during germination revealed a specific order of phospho-ARP appearance, suggesting that this process is under regulation within this period [18].

Anatomical context of CRISP1

• As a relevant evidence for a role of ARP in gamete fusion, the existence of complementary sites for this protein on the surface of human eggs was investigated [19].
• Experiments in which zona-free human oocytes discarded from in vitro fertilization programs were exposed to ARP, fixed, and subjected to indirect immunofluorescence revealed the presence of specific ARP-binding sites on the entire surface of the human egg, in agreement with the fusogenic properties of the human oolema [19].
• An ELISA for SGP28/CRISP-3, a cysteine-rich secretory protein in human neutrophils, plasma, and exocrine secretions [20].
• Cysteine-rich secretory protein (CRISP-3) has been identified as a defense-associated molecule with predominant expression in the salivary gland, pancreas and prostate [21].
• On subcellular fractionation, unlike its mammalian homologue ARP, yARL3 was detected in the soluble fraction but not in the plasma membrane [22].

Associations of CRISP1 with chemical compounds

• Characterization of a human glycoprotein with a potential role in sperm-egg fusion: cDNA cloning, immunohistochemical localization, and chromosomal assignment of the gene (AEGL1) [15].
• In the context of the CRISP promoters a 2-fold induction by R1881 was measured for the CRISP-3 upstream region whereas only limited effects were noted for the CRISP-1 upstream region [23].
• The most striking finding was that recurrent infections, both in LE patients and in those with CBFP reactions and widespread, acral discoid skin lesions, occurred significantly more often in ARP-positive patients [24].
• The acquisition of symmetry axes in the emerging leaf is a process coordinated by hormones (such as auxin and cytokinins) and the expression of classes of genes (such as the knox and the ARP, as1/rs2/phan, genes) [25].
• By the use of ARP assay detecting abasic sites in DNA, we first investigated the activity on the natural DNA substrates containing methylpurines, ethenopurines, or hypoxanthine (Hx) prepared by the conventional methods [26].

Other interactions of CRISP1

• Testicular protein Tpx-1, also known as CRISP-2, is a cysteine-rich secretory protein specifically expressed in the male reproductive tract [27].
• Using electrophoretic mobility shift assay, we identified several elements with differing affinities for the AR at positions -3706, -1270, -1253 and -350 of the CRISP-1 promoter and at positions -369 and -349 of the CRISP-3 promoter [23].
• The first 10-amino-acid sequence showed 60-77% similarity with human cysteine-rich secretory protein-1 precursor, inhibin alpha chain precursor [28].
• Individual therapy is defined and traced from historical origins in psychoanalytic practice with later important modifications by Hilda Bruch and Arthur Crisp [29].
• In an exclusive interview, NHS chief executive Sir Nigel Crisp reveals that 'the BUPAs of this world' will be encouraged to supply care in all areas of elective care where choice is to be offered [30].

Analytical, diagnostic and therapeutic context of CRISP1

• The gene encoding ARP (AEGL1) was mapped by fluorescence in situ hybridization to 6p21.1-p21 [15].
• Sequence alignments revealed conserved residues, motifs, and inserted sequence signatures to define each ARP subfamily [13].
• Purification, crystallization and preliminary X-ray crystallographic analysis of a cysteine-rich secretory protein (CRISP) from Naja atra venom [31].
• The data on the use of ARP as a label in enzyme immunoassay of human IgG are presented [32].
• In a series of autopsy confirmed cases of AD, PD, related disorders, and age-matched controls, DNA fragmentation using the TUNEL method, an array of apoptosis-related proteins (ARP), proto-oncogenes, and activated caspase-3, the key enzyme of late-stage apoptosis, were examined [33].

References