Disease relevance of Il2rb

• These data show that MCF and mMCF-V33 viruses may alter the growth phenotype of a T-cell lymphoma line and suggest that their effect on cell growth may be due to the direct interaction of the MCF envelope glycoprotein with cellular components, perhaps the IL-2 receptor [1].
• RESULTS: The administration of DAB389IL-2 resulted in a one-third reduction in the number of IL-2R-bearing cells and significant weight loss of animals [2].
• OBJECTIVE: We sought to evaluate the effect of the fusion protein DAB389IL-2, which kills cells bearing IL-2R, on delayed-type hypersensitivity and antibody production in Brown Norway rats sensitized with trimellitic anhydride (TMA) [2].
• These results suggest important roles for CD8-positive and IL-2 receptor-bearing T cells in experimental autoimmune thyroiditis [3].
• Differences in IL-10 production and IL-2 receptor expression for stimulated samples in the Hypothermia and Control groups were compared [4].

High impact information on Il2rb

• Those CD8+ T cells responding in the presence of IL-2 were mainly T cell receptor alpha/beta+ IL-2 receptor (alpha chain)+, and a majority had shifted from high to low CD45R expression [5].
• Rat IL-2 receptor, not human IL-2 receptor, was expressed on HTLV+ rat cell lines, as demonstrated by the fact that they expressed antigens reactive with monoclonal antibodies (ART-18) against rat IL-2 receptor, but not with anti-Tac antibodies [6].
• In addition, mRNA for the IL-2 receptor-beta chain was demonstrated by Northern blot analysis using mRNA from primary rat intestinal epithelial cells depleted of detectable contaminating intraepithelial lymphocytes by two cycles of fractionation on Percoll gradients [7].
• The relevance of observations in the IEC-6 cell line to intestinal mucosa in vivo was supported by the demonstration of a gradient of expression of the IL-2 receptor in primary rat intestinal epithelial cells by Western blot analysis [7].
• CONCLUSIONS: The results of this study suggest that NO produced by the inducible NO synthase of Kupffer cells in response to lipopolysaccharide modulates the IL-2 receptor expression and LAK activity of splenocytes [8].

Chemical compound and disease context of Il2rb

• Cyclosporine and dexamethasone inhibit T-lymphocyte MHC class II antigens and IL-2 receptor expression in experimental autoimmune uveitis [9].
• Using a model of chronic pancreatitis induced by dibutyltin dichloride (DBTC) in rats, we have immunohistochemically phenotyped the infiltrating T lymphocytes, CD45RC+ cells, macrophages, as well as the IL-2 receptor as an activation marker during a time course of two months [10].

Biological context of Il2rb

• These results correlate with the rapid kinetics of rejection (4 +/- 1 days), the high number of T cells, the rapid expression of markers of activation (IL-2 receptor alpha-chain and class II receptor), and the strong deposit of IgG Abs in rejected xenografts [11].
• This finding underscores the pivotal role of the IL-2 receptor in the modulation of cellular immunity by glucocorticoids [12].
• However, co-expression of the IL-7R with the IL-2 receptor gamma chain was able to stimulate gene activation [13].
• Together, these results suggest that in newly formed TcRhigh thymocytes, TcR engagement results in clonal deletion if the IL 2 receptor remains unoccupied but allows clonal expansion if IL 2 is provided [14].
• Reconstitution of a functional interleukin (IL)-7 receptor demonstrates that the IL-2 receptor gamma chain is required for IL-7 signal transduction [13].

Anatomical context of Il2rb

• RESULTS: Kupffer cells with lipopolysaccharide reduced the IL-2 receptor expression and LAK activity of splenocytes [8].
• In summary, depletion of IL-2-receptor-bearing cells affects lymphocyte subsets and immunoglobulin production and it decreases the influx of neutrophils into the lungs 8 h after OA challenge, but it does not significantly inhibit the ER, LR, or increased airway responsiveness after antigen challenge [15].
• PBL and GIC from the same pairs of animals were examined for their phenotypic profile (CD4, CD8, 3.2.3 NK cell marker, IL-2 receptor) [16].
• Untreated rat corneas, at the second postoperative week, presented a large number of helper/inducer T cells, macrophages, IL-2 receptor-expressing cells, and Ia-antigen-expressing cells [17].
• Evidence that monoclonal antibodies against the 55kD subunit of the rat IL-2 receptor do not inhibit the development of suppressor cells generated in mixed lymphocyte culture [18].

Associations of Il2rb with chemical compounds

• 8-bromoguanosine 3',5'-cyclic monophosphate and NO donors decreased the splenocyte LAK activity and IL-2 receptor expression [8].
• The addition of either NG-monomethyl-L-arginine, an inhibitor of NO synthesis, or aminoguanidine, an inhibitor of inducible NO synthase, to the medium reversed the suppression of IL-2 receptor expression and LAK activity by lipopolysaccharide-stimulated Kupffer cells [8].
• The steroid effect was not associated with CD28, IL-2 receptor, or transferrin-receptor expression, which were equally upregulated on T cells activated by astrocytes or thymic APC as shown by fluorescence activated cell sorting (FACS) analysis [19].
• The results indicate that the rat IL2 receptor is either a 50-kDa glycoprotein or a 36-kDa molecule, or that both components are part of the receptor molecule [20].
• As a candidate mechanism, we identified the abrogation of a CORT-evoked enhancement of IL-2 receptor expression [12].

Physical interactions of Il2rb

• The binding of [125I]human GH to the lactogen receptor as well as the binding of [125I]IL-2 to the IL-2 receptor were decreased in a dose-dependent manner by GTP, GDP, and the analog guanosine 5'-O-(3-thiotriphosphate) [21].

Regulatory relationships of Il2rb

• Similarly, incubation of NK cells with PRL was not found to induce expression of the alpha chain of the IL-2 receptor (IL-2ra; CD25) on NK cells or other lymphoid cells [22].
• In the present study, IFN-gamma exposure to primary cultures of rat type II epithelial cells (TIIP) upregulated membrane expression of the common gamma-chain of the IL-2 receptor (approximately 2.5- to 4-fold increase) and redistributed receptor affinity in TIIP, as assessed by Western blot, cell, and tissue histochemistry and Scatchard analysis [23].
• Markers of immune activation, such as an increase in IL-2-receptor (from 1% +/- 2% to 21% +/- 13%) and class II (from 20% +/- 9% to 43% +/- 13%) expressing lymphoid cells and induction of ICAM-1 in the graft, were consistent with the overall cellular response [24].

Other interactions of Il2rb

• Inhibition of IL-2-dependent proliferation with an IL-2 receptor blocking antibody also significantly inhibited T-cell migration to 22.2% of controls [25].
• There was no significant expression of IL-2, IL-2 receptor, IL-4, TNFalpha, or IFNgamma [26].
• PGF(2 alpha) stimulated the expression of genes involved in cell signaling such as cell adhesion kinase-beta, ERK3, FRA2, IL-2 receptor, and 14-3-3 proteins [27].
• At 7 days postinfection, lymphocytes from Listeria-infected rats showed significant increases in CD4+ and CD8+ cell counts and the CD8+/CD4+ ratio and exhibited increased production of IFN-gamma and IL-2 receptor expression compared with the noninfected control [28].
• EAE is caused by IL-2 receptor-bearing T cells specific for myelin basic protein (BP) [29].

Analytical, diagnostic and therapeutic context of Il2rb

• Kinetic analysis were consistent with the results of Western blot analysis using an antisera to the 75-kD IL-2 receptor beta chain [7].
• Ab's to the alpha-chain of the IL-2 receptor (anti-CD25) are used clinically to achieve immunosuppression [30].
• The effects on graft function of a full dose or a subclinical dose of CsA, ART-18, a monoclonal antibody (mAb) directed against the IL-2 receptor expressed on activated host cells, and a combination of low-dose CsA and ART-18, have been tested in rat recipients of both heart and kidney allografts [31].
• IL-2R was constitutively expressed in both isograft and allograft and was not a reliable predictor of rejection [32].
• The 53,000 MW protein was identified as the rat IL-2 receptor (IL-2R) alpha-chain by immune precipitation [33].

References