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Chemical Compound Review

HEPES     2-[4-(2- hydroxyethyl)piperazin-1...

Synonyms: Prestwick_256, HEPES solution, AG-G-91129, AG-G-91563, CHEBI:42334, ...
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Disease relevance of HEPES


High impact information on HEPES


Chemical compound and disease context of HEPES


Biological context of HEPES


Anatomical context of HEPES

  • The simian guartan malaria parasite Plasmodium inui (OS strain) was cultured in a continuous flow system with rhesus monkey erythrocytes and RPMI 1640nmedium supplemented with Hepes buffer and rhesus serum [17].
  • EDTA (5 mM) moderately inhibited the binding of insulin to adipocytes in Krebs-Henseleit Hepes buffer [18].
  • Lymphocytes from 24 normal and 24 mild asthmatic subjects who had no drugs for at least 2 weeks were separated on Ficoll-hypaque and incubated in medium 199 with Hepes buffer [19].
  • Hepes buffer is being added to some plasmas and to some reagents used in clotting tests [14].
  • Sheets of epithelial cells are placed in 6 ml of an enzymatic solution containing collagenase, 0.2% bovine serum albumin, 0.04% soya bean trypsin inhibitor, 0.06 ml of 1 M Hepes buffer for 3 h at 37 degrees C. The cells are gently pipetted during the 3-h period, yielding a suspension of viable cells [20].

Associations of HEPES with other chemical compounds

  • Blood-free glomeruli were isolated from kidneys of male, Sprague-Dawley rats using standard sieving techniques, then incubated for one hour at 37 degrees C, pH 7.4, pO2 approximately 500 torr, in Krebs bicarbonate/Hepes buffer containing 5 or 30 mM glucose [21].
  • Neurons with a relatively high pHi in Hepes buffer continued to be more alkaline (by approximately 0.2 pH units) in CO2/HCO3-. 7 [22].
  • The activity of mushroom tyrosinase towards a representative series of phenolic and diphenolic substrates structurally related to tyrosine has been investigated in a mixed solvent of 34.4% methanol-glycerol (7:1, v/v) and 65.6% (v/v) aqueous 50 mM Hepes buffer at pH 6.8 at various temperatures [23].
  • Lead acetate induced DNA strand breakage in 10 mM of Hepes buffer, pH 6.8, in a time- and dose-dependent manner [24].
  • Whereas sedimentation equilibrium distributions for phosphorylase b in 40 mM Hepes buffer (pH 6.8) supplemented with 1 mM AMP signify a lack of chemical equilibrium attainment, those in buffer supplemented additionally with potassium sulfate conform with the requirements of a dimerizing system in chemical as well as sedimentation equilibrium [25].

Gene context of HEPES

  • When Hepes buffer with a low oxygen content was used in cell isolation, pure cultures showed significantly lower GR and GPx activities than those first mentioned.(ABSTRACT TRUNCATED AT 250 WORDS)[26]
  • Almost quantitative+ (90%) entrapment (entrapment percentage defined as absolute entrapment (IU t-PA/mumol PL) divided by total incubation ratio (IU t-PA/mumol PL), times 100%) was obtained in Hepes buffer pH 7.5, devoid of arginine, with low ionic strength [27].
  • The bicarbonate-independent stability constant for Bi3+ binding (K*) to hTF/2N was determined to be log K* 18.9+/-0.2 in 5 mM bicarbonate/10 mM Hepes buffer at 310 K, pH7 [28].
  • In N-2-hydroxyethyl-N'-2-ethanesulfonic acid (Hepes) buffer, the oxidation product 2-iminium-4-pentenoate predominantly reacts to form 2-amino-2,4-pentadienoate, a strong noncovalent inhibitor of D-amino-acid oxidase [29].
  • Incubation of Leydig cells in the defined culture medium Dulbecco's Modified Eagles-Ham's F-12 (1:1, vol/vol) supplemented with 15 mM Hepes buffer, transferrin, insulin, and epidermal growth factor (DHG:F12 + Hepes + TIE) in either the presence or absence of 0.5% fcs yielded functional Leydig cells for longer intervals in culture [30].

Analytical, diagnostic and therapeutic context of HEPES

  • Etheno-AMP in Hepes buffer, pH 7.4, at 22 degrees, was added to confluent monolayers of BPAEC; samples of supernatant were collected after various intervals, and E-AMP and E-Ado were quantitated by HPLC [31].
  • [400 microliters (group 1) and 150 microliters (group 2] of 140 mM NaCl/15 mM Hepes buffer, pH 7.2) and evaluated the effects of electroporation volume on growth and differentiation [32].
  • K influx, measured by 86Rb uptake, was increased in hemodialysis patients from 25.5 +/- 0.6 to 47.3 +/- 3.4 nmol/10(9) cells/h (n = 4, p < 0.01), when the medium osmolarity of Hepes buffer was decreased by 100 mosm/kg H2O [33].
  • The quality control of insulin radioreceptor assay for human erythrocytes is based on the storage of erythrocyte preparations in Hepes buffer of pH 8.0, containing 10 g/l of albumin and 20 mmol/l of glucose [34].
  • Nine representative pharmaceuticals listed in the biopharmaceutical classification system (BCS) were chosen as the candidate compounds and Hank's balanced salt solution with Hepes buffer (HBSS-Hepes buffer) was used as the cell-culture medium in an effort to study permeability of chemicals through cell monolayers [35].


  1. E. coli Dam activity in Hepes buffer asks for a new unit definition. Hülsmann, K.H., Bergerat-Coulaud, A., Hahn, U. Nucleic Acids Res. (1990) [Pubmed]
  2. The structure of Rhodothermus marinus Cel12A, a highly thermostable family 12 endoglucanase, at 1.8 A resolution. Crennell, S.J., Hreggvidsson, G.O., Nordberg Karlsson, E. J. Mol. Biol. (2002) [Pubmed]
  3. Phosphoinositide 3-kinase accelerates necrotic cell death during hypoxia. Aki, T., Mizukami, Y., Oka, Y., Yamaguchi, K., Uemura, K., Fujimiya, T., Yoshida, K. Biochem. J. (2001) [Pubmed]
  4. Silver nanoparticles fabricated in Hepes buffer exhibit cytoprotective activities toward HIV-1 infected cells. Sun, R.W., Chen, R., Chung, N.P., Ho, C.M., Lin, C.L., Che, C.M. Chem. Commun. (Camb.) (2005) [Pubmed]
  5. Involvement of fibrinogen in protamine-induced pulmonary hypertension. Sogawa, M., Mohammad, S.F. J. Surg. Res. (1997) [Pubmed]
  6. Cultivation in vitro of the vivax-type malaria parasite Plasmodium cynomolgi. Nguyen-Dinh, P., Gardner, A.L., Campbell, C.C., Skinner, J.C., Collins, W.E. Science (1981) [Pubmed]
  7. Continuous in vitro propagation of the malaria parasite Plasmodium vivax. Golenda, C.F., Li, J., Rosenberg, R. Proc. Natl. Acad. Sci. U.S.A. (1997) [Pubmed]
  8. Glutamate and non-glutamate receptor mediated toxicity caused by oxygen and glucose deprivation in organotypic hippocampal cultures. Newell, D.W., Barth, A., Papermaster, V., Malouf, A.T. J. Neurosci. (1995) [Pubmed]
  9. Crystal structure of the "cab"-type beta class carbonic anhydrase from the archaeon Methanobacterium thermoautotrophicum. Strop, P., Smith, K.S., Iverson, T.M., Ferry, J.G., Rees, D.C. J. Biol. Chem. (2001) [Pubmed]
  10. Structure of the human erythrocyte insulin receptor. Ward, G.M., Harrison, L.C. Diabetes (1986) [Pubmed]
  11. Hypoxia in fibroblast cultures. 4. Cell density, glucose utilization and acid mucopolysaccharides in 1% O2 concentration. Kittlick, P.D. Experimentelle Pathologie. (1977) [Pubmed]
  12. Phosphorylation of ribosomal proteins during meiotic maturation and following activation in starfish oocytes: its relationship with changes of intracellular pH. Peaucellier, G., Picard, A., Robert, J.J., Capony, J.P., Labbe, J.C., Doree, M. Exp. Cell Res. (1988) [Pubmed]
  13. Product catalyzes the deamidation of D145N dehalogenase to produce the wild-type enzyme. Xiang, H., Dong, J., Carey, P.R., Dunaway-Mariano, D. Biochemistry (1999) [Pubmed]
  14. The effects of hepes buffer on clotting tests, assay of factors V and VIII and on the hydrolysis of esters by thrombin and thrombokinase. Roberts, P.S., Hughes, H.N., Fleming, P.B. Thromb. Haemost. (1976) [Pubmed]
  15. Effects of mannitol or catalase on the generation of reactive oxygen species leading to DNA damage by Chromium(VI) reduction with ascorbate. Tsou, T.C., Lai, H.J., Yang, J.L. Chem. Res. Toxicol. (1999) [Pubmed]
  16. Determination of organophosphorous and carbamate insecticides by flow injection analysis. Kumaran, S., Tran-Minh, C. Anal. Biochem. (1992) [Pubmed]
  17. Cultivation in vitro of the quartan malaria parasite Plasmodium inui. Nguyen-Dinh, P., Campbell, C.C., Collins, W.E. Science (1980) [Pubmed]
  18. Effects of divalent cations on the regulation of insulin-sensitive glucose transport and cAMP phosphodiesterase in adipocytes. Insulin-like effects of divalent cations. Ueda, M., Robinson, F.W., Smith, M.M., Kono, T. J. Biol. Chem. (1984) [Pubmed]
  19. Effect of beta adrenergic agonist, prostaglandins, and cortisol on lymphocyte levels of cyclic adenosine monophosphate and glycogen: abnormal lymphocytic metabolism in asthma. Lee, T.P., Busse, W.W., Reed, C.E. J. Allergy Clin. Immunol. (1977) [Pubmed]
  20. Enrichment of subpopulations of respiratory epithelial cells using flow cytometry. Aitken, M.L., Villalon, M., Verdugo, P., Nameroff, M. Am. J. Respir. Cell Mol. Biol. (1991) [Pubmed]
  21. Diabetes-induced glomerular dysfunction: links to a more reduced cytosolic ratio of NADH/NAD+. Tilton, R.G., Baier, L.D., Harlow, J.E., Smith, S.R., Ostrow, E., Williamson, J.R. Kidney Int. (1992) [Pubmed]
  22. pH regulation in single CA1 neurons acutely isolated from the hippocampi of immature and mature rats. Bevensee, M.O., Cummins, T.R., Haddad, G.G., Boron, W.F., Boyarsky, G. J. Physiol. (Lond.) (1996) [Pubmed]
  23. Mechanistic insight into the activity of tyrosinase from variable-temperature studies in an aqueous/organic solvent. Granata, A., Monzani, E., Bubacco, L., Casella, L. Chemistry (Weinheim an der Bergstrasse, Germany) (2006) [Pubmed]
  24. Singlet oxygen is the major species participating in the induction of DNA strand breakage and 8-hydroxydeoxyguanosine adduct by lead acetate. Yang, J.L., Wang, L.C., Chang, C.Y., Liu, T.Y. Environ. Mol. Mutagen. (1999) [Pubmed]
  25. Ultracentrifugal studies of the effect of molecular crowding by trimethylamine N-oxide on the self-association of muscle glycogen phosphorylase b. Chebotareva, N.A., Harding, S.E., Winzor, D.J. Eur. J. Biochem. (2001) [Pubmed]
  26. Glutathione dependent detoxication in adult rat hepatocytes under various culture conditions. Mertens, K., Rogiers, V., Vercruysse, A. Arch. Toxicol. (1993) [Pubmed]
  27. The preparation of tissue-type Plasminogen Activator (t-PA) containing liposomes: entrapment efficiency and ultracentrifugation damage. Heeremans, J.L., Gerritsen, H.R., Meusen, S.P., Mijnheer, F.W., Gangaram Panday, R.S., Prevost, R., Kluft, C., Crommelin, D.J. Journal of drug targeting. (1995) [Pubmed]
  28. N-lobe versus C-lobe complexation of bismuth by human transferrin. Sun, H., Li, H., Mason, A.B., Woodworth, R.C., Sadler, P.J. Biochem. J. (1999) [Pubmed]
  29. Sequence of reactions which follows enzymatic oxidation of allylglycine. Marcotte, P., Walsh, C. Biochemistry (1978) [Pubmed]
  30. Primary culture of purified Leydig cells isolated from adult rat testes. Browning, J.Y., Heindel, J.J., Grotjan, H.E. Endocrinology (1983) [Pubmed]
  31. A simple assay for ecto-5'-nucleotidase using intact pulmonary artery endothelial cells. Effect of endotoxin-induced cell injury. Bonitati, A.E., Agarwal, K.C., Rounds, S. Biochem. Pharmacol. (1993) [Pubmed]
  32. Low sample volume causes differentiation in human rhabdomyosarcoma cell line RD subjected to electroporation. Aránega, A., Marchal, J.A., Melguizo, C., Prados, J., Aránega, A.E., Vélez, C., Fernández, J.E., Arena, N., Alvarez, L. Cell. Mol. Biol. (Noisy-le-grand) (1996) [Pubmed]
  33. Enhanced volume-sensitive K flux in patients on chronic hemodialysis. Furuya, H., Tabei, K., Asano, Y. Nephron (1994) [Pubmed]
  34. Quality control of insulin radioreceptor assay for human erythrocytes. Effect of ageing of mono-125I-Tyr-A14-insulin preparation. Marttinen, A., Koivula, T., Jokela, H., Lehtinen, M., Pasternack, A. Scand. J. Clin. Lab. Invest. (1984) [Pubmed]
  35. Direct analysis of nine pharmaceuticals in culture media by use of cartridge separation with electrospray mass spectrometric detection. Li, X.F., Ma, M., Tam, Y.K. Analytical and bioanalytical chemistry. (2002) [Pubmed]
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