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Gene Review

Nt5e  -  5' nucleotidase, ecto

Mus musculus

Synonyms: 2210401F01Rik, 5'-NT, 5'-nucleotidase, AI447961, CD73, ...
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Disease relevance of Nt5e


High impact information on Nt5e


Chemical compound and disease context of Nt5e


Biological context of Nt5e


Anatomical context of Nt5e


Associations of Nt5e with chemical compounds

  • Guided by previous work indicating that hypoxia-induced vascular leakage is, at least in part, controlled by adenosine, we generated mice with a targeted disruption of the third coding exon of Cd73 to test the hypothesis that CD73-generated extracellular adenosine functions in an innate protective pathway for hypoxia-induced vascular leakage [1].
  • CD73(+/+) animals challenged with bleomycin and supplemented with exogenous nucleotidase also exhibited reduced inflammation [2].
  • The murine cDNA, encoding the purine catabolic enzyme, ecto-5'-nucleotidase (NT), was cloned and the tissue-specific distribution of both the mRNA and enzyme activity was examined [10].
  • To identify the source(s) of extracellular adenosine, we examined the effects of dipyridamole, an inhibitor of nucleoside transport, and alpha,beta-methyleneadenosine 5'-diphosphate (AOPCP), an inhibitor of ecto-5'-nucleotidase, on AVP-stimulated IscCl [14].
  • The results of the experiments reported here provide three distinct lines of evidence that adenosine results from the ecto-5'-nucleotidase- mediated conversion of adenine nucleotides to adenosine [12].
  • We conclude that CD39 and CD73 are surface markers of T reg cells that impart a specific biochemical signature characterized by adenosine generation that has functional relevance for cellular immunoregulation [15].
  • The A(2B) receptor is a likely target of CD73-generated adenosine, because it is the only adenosine receptor expressed on the HEV-like cell line KOP2.16 and it is up-regulated by TNF-alpha [16].

Other interactions of Nt5e


Analytical, diagnostic and therapeutic context of Nt5e


  1. Crucial role for ecto-5'-nucleotidase (CD73) in vascular leakage during hypoxia. Thompson, L.F., Eltzschig, H.K., Ibla, J.C., Van De Wiele, C.J., Resta, R., Morote-Garcia, J.C., Colgan, S.P. J. Exp. Med. (2004) [Pubmed]
  2. Ecto-5'-nucleotidase (CD73)-mediated adenosine production is tissue protective in a model of bleomycin-induced lung injury. Volmer, J.B., Thompson, L.F., Blackburn, M.R. J. Immunol. (2006) [Pubmed]
  3. Ecto-5'-nucleotidase. II. Effect of 12-O-tetradecanoylphorbol 13-acetate on the expression of enzyme in normal and neoplastic B-cell lines. Hibi, T., SenGupta, S., MacKay, A., Gelfand, E.W., Chechik, B.E. J. Natl. Cancer Inst. (1984) [Pubmed]
  4. Transcriptional effects of estrogen on neuronal neurotensin gene expression involve cAMP/protein kinase A-dependent signaling mechanisms. Watters, J.J., Dorsa, D.M. J. Neurosci. (1998) [Pubmed]
  5. Ecto-5'-nucleotidase expression during human B cell development. An explanation for the heterogeneity in B lymphocyte ecto-5'-nucleotidase activity in patients with hypogammaglobulinemia. Thompson, L.F., Ruedi, J.M., O'Connor, R.D., Bastian, J.F. J. Immunol. (1986) [Pubmed]
  6. CD1d1 mutant mice are deficient in natural T cells that promptly produce IL-4. Mendiratta, S.K., Martin, W.D., Hong, S., Boesteanu, A., Joyce, S., Van Kaer, L. Immunity (1997) [Pubmed]
  7. Metabolism of purines in macrophages. Effect of functional state of the cells. Soberman, R.J., Karnovsky, M.L. J. Exp. Med. (1980) [Pubmed]
  8. Impairment of tubuloglomerular feedback regulation of GFR in ecto-5'-nucleotidase/CD73-deficient mice. Castrop, H., Huang, Y., Hashimoto, S., Mizel, D., Hansen, P., Theilig, F., Bachmann, S., Deng, C., Briggs, J., Schnermann, J. J. Clin. Invest. (2004) [Pubmed]
  9. Trypanosoma cruzi: increased 5'-nucleotidase activity associated with dysfunction of adrenergic receptors in acutely infected albino Swiss mice. Fretes, R.E., Paglini, P., Fernández, A.R., Enders, J., de Fabro, S.P. J. Parasitol. (1999) [Pubmed]
  10. Murine ecto-5'-nucleotidase (CD73): cDNA cloning and tissue distribution. Resta, R., Hooker, S.W., Hansen, K.R., Laurent, A.B., Park, J.L., Blackburn, M.R., Knudsen, T.B., Thompson, L.F. Gene (1993) [Pubmed]
  11. Targeted disruption of cd73/ecto-5'-nucleotidase alters thromboregulation and augments vascular inflammatory response. Koszalka, P., Ozüyaman, B., Huo, Y., Zernecke, A., Flögel, U., Braun, N., Buchheiser, A., Decking, U.K., Smith, M.L., Sévigny, J., Gear, A., Weber, A.A., Molojavyi, A., Ding, Z., Weber, C., Ley, K., Zimmermann, H., Gödecke, A., Schrader, J. Circ. Res. (2004) [Pubmed]
  12. Methotrexate and sulfasalazine promote adenosine release by a mechanism that requires ecto-5'-nucleotidase-mediated conversion of adenine nucleotides. Morabito, L., Montesinos, M.C., Schreibman, D.M., Balter, L., Thompson, L.F., Resta, R., Carlin, G., Huie, M.A., Cronstein, B.N. J. Clin. Invest. (1998) [Pubmed]
  13. CD73 and Ly-6A/E distinguish in vivo primed but uncommitted mouse CD4 T cells from type 1 or type 2 effector cells. Yang, L., Kobie, J.J., Mosmann, T.R. J. Immunol. (2005) [Pubmed]
  14. Adenosine inhibits arginine vasopressin-stimulated chloride secretion in a mouse IMCD cell line (mIMCD-K2). Moyer, B.D., McCoy, D.E., Lee, B., Kizer, N., Stanton, B.A. Am. J. Physiol. (1995) [Pubmed]
  15. Adenosine generation catalyzed by CD39 and CD73 expressed on regulatory T cells mediates immune suppression. Deaglio, S., Dwyer, K.M., Gao, W., Friedman, D., Usheva, A., Erat, A., Chen, J.F., Enjyoji, K., Linden, J., Oukka, M., Kuchroo, V.K., Strom, T.B., Robson, S.C. J. Exp. Med. (2007) [Pubmed]
  16. CD73-generated adenosine restricts lymphocyte migration into draining lymph nodes. Takedachi, M., Qu, D., Ebisuno, Y., Oohara, H., Joachims, M.L., McGee, S.T., Maeda, E., McEver, R.P., Tanaka, T., Miyasaka, M., Murakami, S., Krahn, T., Blackburn, M.R., Thompson, L.F. J. Immunol. (2008) [Pubmed]
  17. Macrophages selectively stimulate ecto-5'-nucleotidase activity of cultured mesangial cells. Stefanovic, V., Savic, V., Vlahovic, P., Ardaillou, N., Ardaillou, R. Kidney Int. (1989) [Pubmed]
  18. The interstitial response to renal injury: fibroblast-like cells show phenotypic changes and have reduced potential for erythropoietin gene expression. Maxwell, P.H., Ferguson, D.J., Nicholls, L.G., Johnson, M.H., Ratcliffe, P.J. Kidney Int. (1997) [Pubmed]
  19. Adenosine levels in the postimplantation mouse uterus: quantitation by HPLC-fluorometric detection and spatiotemporal regulation by 5'-nucleotidase and adenosine deaminase. Blackburn, M.R., Gao, X., Airhart, M.J., Skalko, R.G., Thompson, L.F., Knudsen, T.B. Dev. Dyn. (1992) [Pubmed]
  20. TCR V alpha 24 and V beta 11 coexpression defines a human NK1 T cell analog containing a unique Th0 subpopulation. Prussin, C., Foster, B. J. Immunol. (1997) [Pubmed]
  21. Ly-6C+ natural T (NT) cells mediate immune surveillance against NK-sensitive and NK-resistant transplantable tumours in certain strains of mice. Martiniello, R., Burton, R.C., Smart, Y.C. Int. J. Cancer (1996) [Pubmed]
  22. Effect of phosphate esters, nucleotides and nucleosides on 5'-nucleotidase of cultured mouse macrophages. Lazdins, J., Karnovsky, M.L. J. Cell. Physiol. (1978) [Pubmed]
  23. The increased ecto-5'-nucleotidase activity in muscle, heart and liver of laminin alpha2-deficient mice is not caused by an elevation in the mRNA content. Morote-García, J.C., Sánchez Del Campo, L.F., Campoy, F.J., Vidal, C.J., Muñoz-Delgado, E. Int. J. Biochem. Cell Biol. (2006) [Pubmed]
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