Disease relevance of Omp

• Olfactory marker protein (OMP) genomic clones were isolated from a Charon 4A phage lambda rat genomic library [1].
• Hypothyroidism decreased the growth in density by 25 and 30%, while total number of OMP(+) neurons were reduced by 40 and 70% in the 25- and 90-day-old animals, respectively [2].

High impact information on Omp

• An 11-kb chimeric gene was constructed in which the coding region for OMP was replaced with that for Thy-1 [1].
• The presence of OMP mRNA in olfactory bulb was confirmed by in vitro translation and immunoprecipitation [3].
• OMP cDNA clones were detected by using a 17-base oligonucleotide probe that contained all 16 possible sequences coding for a known partial amino acid sequence of rat OMP [3].
• cDNA clones corresponding to mRNA for rat olfactory marker protein (OMP) were isolated from a cDNA library [3].
• These results suggest either that a previously undescribed population of neurons in the olfactory bulb synthesize OMP or that OMP mRNA is transported to the bulb by axonal transport [3].

Biological context of Omp

• The library was constructed from olfactory mucosa poly(A)+ RNA enriched for OMP mRNA and cloned into a pBR322-derived plasmid, pMG5 [3].
• Studies with transgenic mice indicate that a 0.3-kb fragment of the OMP gene containing one Olf-1 motif is sufficient for olfactory tissue-specific expression of the reporter gene [4].
• We report characterization of several domains within the 5' flanking region of the olfactory marker protein (OMP) gene that may participate in regulating transcription of this and other olfactory neuron-specific genes [4].
• The functional significance that these dynamic regions of OMP have in modulating olfactory signal transduction is discussed [5].
• Dual color FISH analysis of tumor cells confirmed that chromosome regions containing Omp and Jak2 were amplified and were situated in long marker chromosomes showing an aberrant banding pattern [6].

Anatomical context of Omp

• The OMP expression of VRNs was induced not only by AOB neurons but also by neurons of other parts of the central nervous system (CNS) [7].
• By electron microscopy, the development of microvilli in VRNs was found to occur coincidentally with OMP expression in vitro [7].
• The region of the epithelium containing the B50/GAP43-positive cells is virtually devoid of OMP-positive neurons [8].
• Explants of olfactory mucosa cultured with other tissues (cerebrum, cerebellum, cervical spinal cord, and heart) contained approximately the same amount of OMP as explants of mucosa alone; i.e., these other tissues did not enhance OMP levels [9].
• The newly-formed sensory neurons differentiate sufficiently to produce olfactory marker protein, without apparently contacting central nervous tissue [10].

Associations of Omp with chemical compounds

• In addition, the immunoprecipitated product comigrated with OMP on NaDodSO4/polyacrylamide gels and on HPLC [11].
• In contrast to axonally transported vasopressin and oxytocin mRNAs, which are deficient in their 3' polyA tails, axonal OMP RNA fractionated as polyA+ [12].
• Dopamine also stimulated differentiation of surviving 13.S.1.24 cells into bipolar olfactory marker protein-immunoreactive neurons [13].
• GnRH was never co-localized with carnosine/OMP in the same migratory cells [14].
• Among these cell types are the ensheathing cells, the luteinizing hormone-releasing hormone-producing cells and the olfactory marker protein-immunoreactive cells [15].

Physical interactions of Omp

• Olfactory marker protein immunohistochemistry and the anterograde transport of horseradish peroxidase as indices of damage to the olfactory epithelium [16].

Regulatory relationships of Omp

• Number of olfactory marker protein-containing receptor cells is influenced by developmental stage of the olfactory bulb [17].

Other interactions of Omp

• A late effect of the neonatal testosterone propionate treatment on netrin-1, growth-associated protein, olfactory marker protein, collapsin response mediator proteins 1, 3, 4 and cypin gene expression was observed [18].
• In the present study, we analysed the structural features of these three neuron groups using confocal laser scanning light microscopy, focusing on their dendritic arborization pattern, especially on their close apposition to olfactory receptor terminals labeled by olfactory marker protein [19].
• TGF-alpha and olfactory marker protein enhance mitosis in rat olfactory epithelium in vivo [20].
• In developing tissues, IGF-IR staining co-localized with that for olfactory marker protein and growth associated protein GAP-43, but to a lesser extent with synaptophysin [21].

Analytical, diagnostic and therapeutic context of Omp

• Molecular cloning and sequencing of a cDNA for olfactory marker protein [3].
• This has been determined by fast atom bombardment mass spectrometry of the amino terminal dodecapeptide isolated following BrCN cleavage of the OMP [22].
• OMP RNA was detected by in situ hybridization at the light microscope level in axons and in terminals [12].
• Following denervation, both OMP and synapsin II immunoreactivities were diminished, and continued to colocalize in regions retaining immunoreactivity [23].
• Results of radioimmunoassay on the two groups of cultures showed that about twice as much OMP was present in organ cultures grown with presumptive olfactory bulb [9].

References