Disease relevance of IL2

• In animal immune experiment, Newcastle disease vaccine was mixed, respectively, with two cCHMIs and IL-2 to vaccinate 15-day-old chicken in experimental groups [1].
• We examined the ability of a set of cloned chicken ovalbumin (cOVA)-specific, Id-restricted, T cell hybridomas to produce interleukin-2 in response to cOVA presented by the Ia+ B cell lymphoma line, A20-2J [2].
• TIL exhibited high proliferative responses to T cell mitogens (concanavalin A, phytohemagglutinin) and IL 2, but only weak responses to the B cell mitogen LPS from Salmonella typhimurium [3].
• The mitogenic response to Con A and the production of T cell growth factor or interleukin 2 (IL 2) by splenic and peripheral blood lymphocytes of obese strain (OS) chickens with spontaneous autoimmune thyroiditis have been investigated [4].
• Interleukin-2 gene transduction into freshly isolated lung adenocarcinoma cells with adenoviral vectors [5].

High impact information on IL2

• T-cell activation with concanavalin A plus interleukin-2 induced spleen cells to express this gene with kinetics correlating with the acquisition of cytolytic capacity [6].
• Hen egg lysozyme 52-61-specific CD4+ T cells responded by interleukin 2 (IL-2) secretion to any peptide containing this epitope regardless of length of NH2- and COOH-terminal composition [7].
• Peptides that failed to induce IL-2 secretion in the CD4- variants nevertheless induced strong tyrosine phosphorylation of CD3 zeta [7].
• Expression of N-cadherin and an interleukin 2 receptor/cadherin chimera in SW480 cells relocated beta-catenin from the nucleus to the plasma membrane and reduced transactivation [8].
• Altogether, these data indicate that the drop of IL-2 production and T-cell proliferation, as well as the up regulation of IL-4 and IFN-gamma production, are complex manifestations of an evolving T-cell response [9].

Chemical compound and disease context of IL2

• RRR-alpha-tocopheryl succinate induced interleukin-2 production by avian splenic T lymphocytes and murine EL-4 thymic lymphoma cells [10].
• RRR-alpha-tocopheryl succinate (vitamin E succinate) was studied for its effects on interleukin-2 (IL-2) production by chicken splenic derived T lymphocytes and murine EL-4 thymic lymphoma cells [10].

Biological context of IL2

• A Taq I polymorphism was identified which enabled chicken IL2 to be mapped to chromosome 4, linked to ANX5, with synteny with mouse chromosome 3 and human chromosome 4 [11].
• The mammalian NF-kappaB and octamer binding sites seem to be absent, although there are alternative potential NF-kappaB and octamer-binding elements in the chicken IL2 promoter, in close proximity to their mammalian homologues [11].
• The exon:intron structure of chicken IL2 corresponds almost exactly to those of mammalian IL2s with the exceptions of exon 2 and introns 2 and 3 which are shorter [11].
• There was an antigen-specific dose-dependent down regulation of IL-2 production and T-cell proliferation in lymph node T cells [9].
• Thus, whereas the TCR still recognized and bound to the MHC class II-peptide complex resulting in protein phosphorylation, this interaction failed to induce effective signal transduction manifested by IL-2 secretion [7].

Anatomical context of IL2

• Immunologic synergism with IL-2 and effects of cCHMIs on mRNA expression of IL-2 and IFN-gamma in chicken peripheral T lymphocyte [1].
• IL-2, cloned in several mammalian species, plays a critical role in immune system function [12].
• Murine B lymphocytes which were prestimulated with anti-lg (Fab')2 antibody could readily be induced by semipurified BCGF (containing some IL2 activity) to incorporate thymidine [13].
• In this work, we have designed a system that lacks exogenous IL 2 by using as our source of help, antigen-specific helper molecules derived from helper T cells [14].
• Soluble suppressor factors can be detected in conditioned media from supernatants of Con A-stimulated cocultures containing suppressor monocytes, but their suppressor activity is partially opposed by stimulatory factors, possibly interleukin 2, also present in supernatants [15].

Associations of IL2 with chemical compounds

• Chicken IL2 binds specifically to thymocytes and activated T cells and its action upon T blast cells cannot be blocked by cyclosporin A [16].
• In contrast to data from mammals, we found no evidence that interleukin 2 elevates chicken corticosterone [17].
• Effect of triiodothyronine and in vitro growth hormone on avian interleukin-2 [18].
• Attempts to reverse VES's antiproliferative effects by addition of exogenous interleukin-2 or addition of sodium selenite, an enhancer of interleukin-2 receptors, failed [19].
• Production of IL-2 by splenocytes was higher as indicated by higher stimulation indices in ascorbic acid supplemented group [20].

Regulatory relationships of IL2

• BACKGROUND: The proto-oncogene product c-Rel is a Rel/NF-kappaB family transcription factor that plays a critical role in lymphoid cell development and mediates CD28-induced expression of interleukin 2 (IL-2) [21].

Other interactions of IL2

• After cultivation of 7h, the expression of IL-2 mRNA in the cell, 24h, IFN-gamma mRNA, were determined by semi-quantitative RT-PCR assay [1].
• The results show that this cytokine is chicken IL2 and not IL15 [11].
• Priming by recombinant chicken interleukin-2 induces selective expression of IL-8 and IL-18 mRNA in chicken heterophils during receptor-mediated phagocytosis of opsonized and nonopsonized Salmonella enterica serovar enteritidis [22].
• The virus-specific cells produced interferon- gamma, tumor necrosis factor- alpha, and interleukin-2 [23].
• They expressed cell surface CD8, a putative interleukin-2 receptor, CD45 and a receptor for IgG, but did not express CD4, major histocompatibility complex class II or immunoglobulin [24].

Analytical, diagnostic and therapeutic context of IL2

• The molecular weights of the two biological active peaks found by gel filtration of the native IL2 preparation were in the range 19500-21500 and 9000-11500 [16].
• Failure to alter neonatal transplantation tolerance by the injection of interleukin 2 [25].
• Using PCR, we searched for IL-2 gene sequences in a wide variety of mammals, including marsupials and monotremes, as well as in birds [26].
• After administration of Adex1CAmIL2 to mice (4x10(7) pfu per animal), the expression of murine interleukin-2 in hepatocytes was examined by immunostaining and in situ hybridization, and the natural killer activity of hepatic mononuclear cells was measured [27].
• The stimulatory capacity of the reconstituted peptide-I-Ed complexes adsorbed on the well surface of cell culture plates was then evaluated by measuring interleukin-2 secreted by an HEL 107-116-specific, I-Ed-restricted T cell hybridoma [28].

References