Disease relevance of TF

• Interaction of ruminant transferrins with transferrin receptors in bovine isolates of Pasteurella haemolytica and Haemophilus somnus [1].
• Rapid alterations in transferrin sialylation during sepsis [2].
• Production of transferrin receptors by Histophilus ovis: three of five strains require two signals [3].
• Using the parabolic equation, y = axb, the constants for the relationship between body weight (x) and transferrin turnover (y) were a, 22.845; and b, 0.68 [4].
• The transferrin system in Herdwick sheep, bred at Compton, was investigated with special reference to the susceptibility of the flock to experimentally produced scrapie [5].

High impact information on TF

• Fate of the transferrin receptor during maturation of sheep reticulocytes in vitro: selective externalization of the receptor [6].
• It is proposed that the exocytosis of the approximately 50-nm bodies represents the mechanism by which the transferrin receptor is shed during reticulocyte maturation [7].
• Interleukin 2 stimulated null cells, retained a Leu-11+, Leu-4- phenotype, and expressed only low levels of receptors for IL-2 and transferrin [8].
• The activities include acetylcholinesterase, cytochalasin B binding (glucose transporter) nucleoside binding (i.e. nucleoside transporter), Na+-independent amino acid transport, and the transferrin receptor [9].
• The acylated transferrin receptor in the reticulocyte membrane was specifically precipitated with a monoclonal antibody and was absent from mature red cell membranes [10].

Chemical compound and disease context of TF

• Sepsis is associated with decreased SA content on circulating transferrin and with an increase in blood free SA concentrations [2].

Biological context of TF

• In vitro acylation of the transferrin receptor [10].
• Selective externalization of the transferrin receptor by sheep reticulocytes in vitro. Response to ligands and inhibitors of endocytosis [11].
• We have investigated the phosphorylation of transferrin receptors both in intact sheep reticulocytes and in isolated plasma membranes [12].
• Evidence is presented for the transmembrane nature of the receptor and for the presence of different binding sites for transferrin and these antibodies on the receptor [13].
• Ovine transferrin was identified by its apparent molecular weight in sodium dodecyl sulphate polyacrylamide gel electrophoresis and by its N-terminal amino-acid sequence [14].

Anatomical context of TF

• Hemoglobin released from lysed erythrocytes can restore the transferrin-inhibited growth of C. albicans [15].
• 3. With regard to activation-associated lymphocyte markers, iron significantly enhanced expression of the receptor for transferrin as identified by the monoclonal antibody, OKT9 [16].
• The changes parallel those observed in normal T-cell differentiation and partly reflect alterations in glycosyl transferase activity, altered synthesis of proteins and regulation of cell surface receptors (for transferrin) associated with rapid growth and metabolism [17].
• Neither cAMP nor transferrin had a significant stimulating effect on blastocyst development of 1/8 blastomeres when mKRB plus serum was used as the medium [18].
• In conclusion, secretions by Sertoli cells of the paracrine factor involved in the control of testosterone production by Leydig cells and of transferrin are modified by germ cells [19].

Associations of TF with chemical compounds

• Vesicles containing peptides that comigrate with the transferrin receptor on polyacrylamide gels are released during incubation of sheep reticulocytes, tagged with anti-transferrin-receptor antibodies [6].
• SCE cultures were established from 20-day-old rats and maintained in SF media supplemented with insulin, transferrin, epidermal growth factor (EGF), and bacitracin [20].
• When culture was performed in a serum-free medium containing transferrin and ascorbic acid, the number of cells increased only slightly (1.2-fold) over a 4-day period [21].
• Inclusion of transferrin or retinol in the culture medium increased the production of 5 alpha-reduced metabolites [22].
• Large-format, two-dimensional polyacrylamide gel electrophoresis of ovine periimplantation uterine luminal fluid proteins: identification of aldose reductase, cytoplasmic actin, and transferrin as conceptus-synthesized proteins [23].

Physical interactions of TF

• Synthesis of lactoferrin and transport of transferrin in the lactating mammary gland of sheep [24].

Other interactions of TF

• By 2D gel map comparison and Western blotting, we identified transferrin, secreted by the yolk sac from Day 15, and cytoplasmic actin, one of the most abundant proteins produced by the trophoblast at Day 17 [23].
• Thyroid cells were incubated with TSH and five additives (insulin, somatostatin, growth hormone, transferrin, and glycyl-L-histidyl-L-lysin) for 2-3 weeks and then were exposed to sodium iodide (NaI) or 1-methylimidazole-2-thiol (methimazole, MMI), or both for 72 h [25].
• The three primary embryonic layers and tissues that differentiate from them were tested for the presence of alpha-fetoprotein (AFP), fetuin, albumin, transferrin and alpha 1-antitrypsin using the indirect immunoperoxidase method [26].
• These results suggest that, although lactoferrin is synthesized by mammary gland of the sheep, transferrin comes from blood serum, probably by a receptor-mediated mechanism of transcytosis [24].
• The good linearity (R2=0.998) was also observed between the transferrin concentration (0.5-1.2 g/L) and peak area ratio (IS; beta-lactoglobulin B) with acceptable accuracy (<1.9%) and repeatability ( approximately 10% at 1g/L) [27].

Analytical, diagnostic and therapeutic context of TF

• After washing and detergent lysis in the presence of protease inhibitors, supernatant reticulocyte extracts were analyzed for transferrin receptors by immunoprecipitation with specific ovine receptor antibody raised against denatured rabbit transferrin receptor [28].
• However, with strain 3384Y and depending on the primer pair, tbpA transcripts were detected by RT-PCR predominantly when the RNA was from cells grown under iron-restricted conditions in the presence of bovine Tf [29].
• Positron emission tomography showed no increase in the pulmonary transcapillary escape rate for 68Ga-labeled transferrin or in extravascular lung water (n = 4) [30].
• Sheep thyroid follicles were isolated by incubation with collagenase and differential centrifugation, grown in Coon's modified Ham's F12M medium with the addition of transferrin, glycylhistidyl-lysine, somatostatin (3H), TSH, cortisol and insulin (6H), and maintained in OH (hormone-free) or 3H medium with or without further supplements for 48 h [31].
• Transferrin variants in sheep: separation and characterization by polyacrylamide gel electrophoresis and isoelectric focusing [32].

References