The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
Gene Review

LOC443340  -  actin

Ovis aries

Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of LOC443340

  • Carcinoid valve cusps demonstrated the unusual finding of widespread smooth muscle actin involving the interstitial cells in the periphery of carcinoid nodules; these same cells were also positive for LAP [1].
  • Furthermore, plasma from patients with ARDS secondary to bacterial pneumonia is toxic to SPAEC, and a small but significant contributory role of actin is apparent in these studies [2].
  • Actin released from damaged cells after a variety of tissue injuries appears to be involved in multiple organ dysfunction syndrome [2].
  • However, an E. coli O157:H7 tccP mutant induced typical attaching and effacing lesions in a bovine ligated ileal loop model of infection, suggesting that TccP-independent mechanisms of actin assembly may operate in vivo [3].
  • CONCLUSION: The onset of proteinuria in the PHN model of MN is coincident with complement-dependent alterations in the association of nephrin with the actin cytoskeleton and loss of podocyte slit diaphragm integrity [4].

High impact information on LOC443340


Chemical compound and disease context of LOC443340


Biological context of LOC443340

  • Chimeric transmembrane proteins bearing syk but not src family tyrosine kinase domains are capable of autonomously triggering phagocytosis and redistribution of filamentous actin in COS cells [11].
  • Controls included parallel hybridizations with a 35S-labeled gamma-actin cDNA to detect actin mRNA (positive control) and with 35S-labeled plasmid cDNA (negative control) [12].
  • Purity of smooth muscle cell cultures at each vascular site was assessed by the "hills and valleys" phenotype and by positive immunofluorescence with a smooth muscle actin specific monoclonal antibody [13].
  • Coordinated regulation of synapsin I interaction with F-actin by Ca2+/calmodulin and phosphorylation: inhibition of actin binding and bundling [14].
  • This protein fraction which stimulates FPCL contraction is not related to 1) actin-myosin filaments enhanced contraction by ATP-induced cell contraction, 2) promotion of fibroblast elongation on glass surface or in collagen, or 3) increased cell number by enhanced fibroblast duplication in a collagen matrix [15].

Anatomical context of LOC443340

  • Cytochalasin D, an inhibitor of actin assembly and polymerization, abolished phagocytic activity in both control and beta-interferon-treated macrophages [16].
  • These results imply that in resting platelets and neutrophils, actin is sequestered by T beta 4 as MgATP-G-actin [17].
  • Methylamine protection experiments and immunofluorescence studies suggested that CNF2 enters the cytosol of the target cell through an acidic compartment and induces the reorganization of actin into stress fibers [18].
  • Normal valve cusps were only focally positive for smooth muscle actin and LAP [1].
  • An actin assay which employs the competition between labeled and unlabeled rabbit skeletal muscle actin for DNase I has been developed [19].

Associations of LOC443340 with chemical compounds

  • At low concentrations the incorporation was comparable to that of dimethyl casein and much greater than actin or fibrinogen [20].
  • Western blot analysis of the glomerular extracts showed a significant reduction in the total amount of nephrin and in the fraction of actin-associated nephrin in the PHN group, whereas the amounts in the complement-depleted rats were similar to normal controls [4].
  • In both adult and fetal middle cerebral artery, cytochalasin D-induced inhibition of actin polymerization decreased PHE-induced contraction, to approximately 60 and approximately 40% of control, respectively (despite no significant change in expression level) [21].
  • Actin, MHC, and MHC isoforms were analyzed by SDS-PAGE using 3-20% and 4% polyacrylamide gels, respectively [22].
  • Incubation of sperm in media lacking BSA or methyl-beta-cyclodextrin, Ca(2+), or NaHCO(3), components that are all required for capacitation, prevented actin polymerization as well as capacitation, as assessed by the ability of the cells to undergo the acrosome reaction [23].

Physical interactions of LOC443340

  • Under the conditions of our assay (0.45 microM synapsin I, 4 microM F-actin), half-maximal inhibition of actin binding and bundling by unphosphorylated synapsin I was found with 4.3 and 3.7 microM calmodulin, respectively [14].

Enzymatic interactions of LOC443340

  • The actin binding activity of synapsin I phosphorylated by cAMP-dependent protein kinase or by calmodulin-dependent protein kinase II showed similar sensitivity to calmodulin inhibition to unphosphorylated synapsin I [14].

Regulatory relationships of LOC443340

  • Treatment of cultured cardiac fibroblasts with transforming growth factor-beta (10 ng/ml) induced the expression of alpha-smooth muscle actin, characteristic of the transformation to myofibroblasts, and raised ANP concentrations in the medium [24].
  • Filamin (0.007:actin) also decreased the inhibitory action of caldesmon on actin-activated myosin ATPase and also potentiated the reversal of this inhibition by calmodulin [25].

Other interactions of LOC443340

  • Total RNA was subjected to Northern analysis using specific complementary DNA probes to CRH and POMC, and specific message was normalized to actin mRNA content in each individual sample [26].
  • Day 15 pregnant ewes had lower expression of ER, PR, GAPDH, cyclophilin and actin genes [27].
  • The interactions of vascular smooth muscle caldesmon with actin, tropomyosin, and calmodulin were determined under conditions in which the four proteins can form reconstituted Ca2+-sensitive smooth muscle thin filaments [28].
  • Actin, tropomyosin, caldesmon and calponin were present in molar ratios 14:2:1:0 [29].
  • By 2D gel map comparison and Western blotting, we identified transferrin, secreted by the yolk sac from Day 15, and cytoplasmic actin, one of the most abundant proteins produced by the trophoblast at Day 17 [30].

Analytical, diagnostic and therapeutic context of LOC443340


  1. Serotonin mechanisms in heart valve disease I: serotonin-induced up-regulation of transforming growth factor-beta1 via G-protein signal transduction in aortic valve interstitial cells. Jian, B., Xu, J., Connolly, J., Savani, R.C., Narula, N., Liang, B., Levy, R.J. Am. J. Pathol. (2002) [Pubmed]
  2. Actin-containing sera from patients with adult respiratory distress syndrome are toxic to sheep pulmonary endothelial cells. Erukhimov, J.A., Tang, Z.L., Johnson, B.A., Donahoe, M.P., Razzack, J.A., Gibson, K.F., Lee, W.M., Wasserloos, K.J., Watkins, S.A., Pitt, B.R. Am. J. Respir. Crit. Care Med. (2000) [Pubmed]
  3. Role of intimin-tir interactions and the tir-cytoskeleton coupling protein in the colonization of calves and lambs by Escherichia coli O157:H7. Vlisidou, I., Dziva, F., La Ragione, R.M., Best, A., Garmendia, J., Hawes, P., Monaghan, P., Cawthraw, S.A., Frankel, G., Woodward, M.J., Stevens, M.P. Infect. Immun. (2006) [Pubmed]
  4. Complement mediates nephrin redistribution and actin dissociation in experimental membranous nephropathy. Saran, A.M., Yuan, H., Takeuchi, E., McLaughlin, M., Salant, D.J. Kidney Int. (2003) [Pubmed]
  5. How profilin promotes actin filament assembly in the presence of thymosin beta 4. Pantaloni, D., Carlier, M.F. Cell (1993) [Pubmed]
  6. A role for phosphoinositide 3-kinase in the completion of macropinocytosis and phagocytosis by macrophages. Araki, N., Johnson, M.T., Swanson, J.A. J. Cell Biol. (1996) [Pubmed]
  7. Identical distribution of fluorescently labeled brain and muscle actins in living cardiac fibroblasts and myocytes. McKenna, N., Meigs, J.B., Wang, Y.L. J. Cell Biol. (1985) [Pubmed]
  8. Actin in the photoreceptor connecting cilium: immunocytochemical localization to the site of outer segment disk formation. Chaitin, M.H., Schneider, B.G., Hall, M.O., Papermaster, D.S. J. Cell Biol. (1984) [Pubmed]
  9. Effects of long-term hypoxia and development on cardiac contractile proteins in fetal and adult sheep. Kamitomo, M., Onishi, J., Gutierrez, I., Stiffel, V.M., Gilbert, R.D. J. Soc. Gynecol. Investig. (2002) [Pubmed]
  10. Angiotensin II stimulation of granuloma macrophage phagocytosis and actin polymerization in murine schistosomiasis mansoni. Weinstock, J.V., Kassab, J.T. Cell. Immunol. (1984) [Pubmed]
  11. Clustered syk tyrosine kinase domains trigger phagocytosis. Greenberg, S., Chang, P., Wang, D.C., Xavier, R., Seed, B. Proc. Natl. Acad. Sci. U.S.A. (1996) [Pubmed]
  12. In situ localization of mRNA for the interferon, ovine trophoblast protein-1, during early embryonic development of the sheep. Farin, C.E., Imakawa, K., Roberts, R.M. Mol. Endocrinol. (1989) [Pubmed]
  13. Qualitative and quantitative differences in protein synthesis comparing fetal lamb ductus arteriosus endothelium and smooth muscle with cells from adjacent vascular sites. Rabinovitch, M., Beharry, S., Bothwell, T., Jackowski, G. Dev. Biol. (1988) [Pubmed]
  14. Coordinated regulation of synapsin I interaction with F-actin by Ca2+/calmodulin and phosphorylation: inhibition of actin binding and bundling. Goold, R., Chan, K.M., Baines, A.J. Biochemistry (1995) [Pubmed]
  15. Sheep amniotic fluid has a protein factor which stimulates human fibroblast populated collagen lattice contraction. Rittenberg, T., Longaker, M.T., Adzick, N.S., Ehrlich, H.P. J. Cell. Physiol. (1991) [Pubmed]
  16. Interferon suppresses pinocytosis but stimulates phagocytosis in mouse peritoneal macrophages: related changes in cytoskeletal organization. Wang, E., Michl, J., Pfeffer, L.M., Silverstein, S.C., Tamm, I. J. Cell Biol. (1984) [Pubmed]
  17. Modulation of the interaction between G-actin and thymosin beta 4 by the ATP/ADP ratio: possible implication in the regulation of actin dynamics. Carlier, M.F., Jean, C., Rieger, K.J., Lenfant, M., Pantaloni, D. Proc. Natl. Acad. Sci. U.S.A. (1993) [Pubmed]
  18. Cytotoxic necrotizing factor type 2 produced by virulent Escherichia coli modifies the small GTP-binding proteins Rho involved in assembly of actin stress fibers. Oswald, E., Sugai, M., Labigne, A., Wu, H.C., Fiorentini, C., Boquet, P., O'Brien, A.D. Proc. Natl. Acad. Sci. U.S.A. (1994) [Pubmed]
  19. A DNase I binding/immunoprecipitation assay for actin. Snabes, M.C., Boyd, A.E., Pardue, R.L., Bryan, J. J. Biol. Chem. (1981) [Pubmed]
  20. Identification of a substrate site for liver transglutaminase on the aminopropeptide of type III collagen. Bowness, J.M., Folk, J.E., Timpl, R. J. Biol. Chem. (1987) [Pubmed]
  21. Expression of several cytoskeletal proteins in ovine cerebral arteries: developmental and functional considerations. Zhao, Y., Xiao, H., Long, W., Pearce, W.J., Longo, L.D. J. Physiol. (Lond.) (2004) [Pubmed]
  22. Smooth muscle myosin heavy chain isoforms are developmentally regulated in male fetal and neonatal sheep. Chern, J., Kamm, K.E., Rosenfeld, C.R. Pediatr. Res. (1995) [Pubmed]
  23. Remodeling of the actin cytoskeleton during mammalian sperm capacitation and acrosome reaction. Brener, E., Rubinstein, S., Cohen, G., Shternall, K., Rivlin, J., Breitbart, H. Biol. Reprod. (2003) [Pubmed]
  24. Atrial (ANP) and brain natriuretic peptide (BNP) expression after myocardial infarction in sheep: ANP is synthesized by fibroblasts infiltrating the infarct. Cameron, V.A., Rademaker, M.T., Ellmers, L.J., Espiner, E.A., Nicholls, M.G., Richards, A.M. Endocrinology (2000) [Pubmed]
  25. Filamin and gelsolin influence Ca(2+)-sensitivity of smooth muscle thin filaments. Gusev, N.B., Pritchard, K., Hodgkinson, J.L., Marston, S.B. J. Muscle Res. Cell. Motil. (1994) [Pubmed]
  26. Effect of implantation of dexamethasone adjacent to the paraventricular nucleus on messenger ribonucleic acid for corticotropin-releasing hormone and proopiomelanocortin during late gestation in fetal sheep. Myers, D.A., McDonald, T.J., Dunn, T.G., Moss, G.E., Nathanielsz, P.W. Endocrinology (1992) [Pubmed]
  27. Cell-specific expression of estrogen-responsive genes in the uteri of cyclic, early pregnant and ovariectomized ewes. Ing, N.H., Zhang, Y. Theriogenology (2004) [Pubmed]
  28. The mechanism of Ca2+ regulation of vascular smooth muscle thin filaments by caldesmon and calmodulin. Smith, C.W., Pritchard, K., Marston, S.B. J. Biol. Chem. (1987) [Pubmed]
  29. Properties of calponin isolated from sheep aorta thin filaments. Marston, S.B. FEBS Lett. (1991) [Pubmed]
  30. Large-format, two-dimensional polyacrylamide gel electrophoresis of ovine periimplantation uterine luminal fluid proteins: identification of aldose reductase, cytoplasmic actin, and transferrin as conceptus-synthesized proteins. Lee, R.S., Wheeler, T.T., Peterson, A.J. Biol. Reprod. (1998) [Pubmed]
  31. Identification and characterization of an abundant ovarian interstitial gland protein associated with sexual maturity in rabbits. Washenik, K.J., Dunbar, B.S. Endocrinology (1988) [Pubmed]
  32. Fetal partial urethral obstruction causes renal fibrosis and is associated with proteolytic imbalance. Gobet, R., Bleakley, J., Cisek, L., Kaefer, M., Moses, M.A., Fernandez, C.A., Peters, C.A. J. Urol. (1999) [Pubmed]
  33. Slowed transcription and rapid messenger RNA turnover contribute to a decline in synthesis of ovine trophoblast protein-1 during in vitro culture. Hansen, T.R., Cross, J.C., Farin, C.E., Imakawa, K., Roberts, R.M. Biol. Reprod. (1991) [Pubmed]
  34. A nontoxic diphtheria toxin analogue inhibits neonatal bladder smooth muscle cell proliferation. Kaefer, M., Vemulapalli, S., Freeman, M.R. J. Urol. (2000) [Pubmed]
WikiGenes - Universities