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Gene Review

P2RY2  -  purinergic receptor P2Y, G-protein coupled, 2

Homo sapiens

Synonyms: ATP receptor, HP2U, P2RU1, P2U, P2U purinoceptor 1, ...
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Disease relevance of P2RY2


High impact information on P2RY2

  • Up(4)A is likely to exert vasoconstriction predominantly through P2X1 receptors, and probably also through P2Y2 and P2Y4 receptors [5].
  • Both pharmacological data and the occurrence of selectivity of coupling to second-messenger pathways indicate the existence of multiple members in several of the classes of P2-purinergic receptor subtypes [6].
  • ATP receptor regulation of adenylate cyclase and protein kinase C activity in cultured renal LLC-PK1 cells [7].
  • Mucin secretion was stimulated by extracellular adenosine 5'-triphosphate via P2U receptors, cytosolic calcium increase, and PKC and by taurochenodeoxycholate via cytosolic calcium increase and Ca2+/CaM-kinase II [8].
  • RESULTS: Purinergic receptor agonists ATP and uridine triphosphate stimulated 125I and 86Rb efflux about twofold above basal levels [9].

Chemical compound and disease context of P2RY2


Biological context of P2RY2

  • An increase in cell number was caused by low doses of the nonspecific P2 receptor agonist ATP, the P2Y2 receptor agonist UTP (p<0.001), and the P2Y1 receptor agonist 2MeSADP (p<0.05) [15].
  • These data demonstrate that multiple P2Y receptors (P2Y1, P2Y2, and P2Y4 subtypes) are differentially involved in the regulation of proliferation in human keratinocytes and therefore may be important in wound healing [16].
  • ATP, at high concentrations, induced apoptosis through ligation of P2X7 and P2Y1 receptors; conversely, ATP, at lower concentrations, and UTP stimulated proliferation, probably acting via P2Y2 receptors [17].
  • We have recently cloned from the human genome a new subtype of receptor (tentatively called P2Y4), which is structurally related to the P2U receptor [18].
  • Photolabeling of the 53-kDa protein by [alpha-32P]BzATP was inhibited by ATP but not by UTP, raising the possibility that the P2U receptor may have distinct binding sites for each nucleotide [19].

Anatomical context of P2RY2


Associations of P2RY2 with chemical compounds

  • The P2Y2-receptor is activated by UTP and ATP and blocked by suramin [22].
  • In both cell types, adenosine 5'-triphosphate and uridine 5'-triphosphate induced Cai transients of approximately equal duration, magnitude, and shape, confirming the presence of functional P2Y2 receptors [16].
  • 3. Inositol trisphosphates assays have identified a response typical of the P2Y2 receptor in the 1HAEo- and the 16HBE14o- airway epithelial cell lines which co-express P2Y2 and P2Y6 mRNA [21].
  • Applications with ATP and UTP revealed that luminal membranes of NHNE cells express P2Y2 and P2Y6 receptors and basolateral membranes express P2Y2 receptor [23].
  • For example, the dinucleotide INS 37217 (Up4dC) potently activates the P2Y2 receptor, and the non-nucleotide antagonist A-317491 is selective for P2X(2/3)/P2X3 receptors [24].
  • Collectively, these results suggest that alpha v integrin complexes provide the P2Y2R with access to G12, thereby allowing activation of this heterotrimeric G protein that controls actin cytoskeletal rearrangements required for chemotaxis [25].

Physical interactions of P2RY2

  • Here it is demonstrated that activation of the G protein-coupled P2Y2 receptor (P2Y2R) subtype expressed in human 1321N1 astrocytoma cells enhanced the release of sAPP alpha in a time- and dose-dependent manner [26].

Regulatory relationships of P2RY2


Other interactions of P2RY2

  • Human keratinocytes express multiple P2Y-receptors: evidence for functional P2Y1, P2Y2, and P2Y4 receptors [16].
  • The P2X7 receptor agonist benzoylbenzoyl-adenosine 5'-triphosphate and high concentrations of adenosine 5'-triphosphate (1000-5000 microM) caused a significant reduction in A431 cell number (p<0.001), whereas the P2Y2 receptor agonist uridine 5'-triphosphate caused a significant amount of proliferation (p<0.001) [2].
  • Expressions of P2Y2 and P2Y6 receptors in NHNE cells were further verified by immunoblotting using specific antibodies [23].
  • In conclusion, P2X1, P2Y2, and P2Y6 are the most expressed P2 receptors in SMC and are thus probably mediating the contractile and mitogenic actions of extracellular nucleotides [30].
  • CONCLUSION: This study showed that P2Y2 and P2Y11 receptors were expressed in NHNE cells and that their agonists, UTP and ATPgammaS, act as secretogogues on mucin secretion via Ca2+-dependent pathways [31].

Analytical, diagnostic and therapeutic context of P2RY2

  • The present study was designed to examine the expression and regulation of the P2UR in human granulosa-luteal cells (hGLCs) by RT-PCR and Northern blot analysis [32].
  • A PCR product corresponding to the expected 599-bp P2UR complementary DNA was obtained from hGLCs [32].
  • Purification of HA-tagged P2Y2 receptors from transfected human 1321N1 astrocytoma cells yielded a protein with a molecular size determined by SDS-PAGE to be in the range of 57-76 kDa, which is typical of membrane glycoproteins with heterogeneous complex glycosylation [33].
  • Microfluorometry and confocal laser scanning was used on preparations stained with the Ca2+-sensitive dyes Calcium Green-1 and Fura Red. In myelinating Schwann cells of human and rat nerves, the ATP-induced rise of [Ca2+]i resulted from the activation of a P2Y2 purinoceptor subtype (rank order of potency: UTP > or = ATP >> 2-MeSATP = ADP) [34].
  • Flow cytometry analysis of thymocyte subsets excluded the possibility that the observed increases in P2Y2 receptor mRNA expression were due to the enrichment of steroid-treated cells with an P2Y2 mRNA-rich thymocyte subset [35].


  1. The P2Y2 nucleotide receptor mediates UTP-induced vascular cell adhesion molecule-1 expression in coronary artery endothelial cells. Seye, C.I., Yu, N., Jain, R., Kong, Q., Minor, T., Newton, J., Erb, L., González, F.A., Weisman, G.A. J. Biol. Chem. (2003) [Pubmed]
  2. Expression of purinergic receptors in non-melanoma skin cancers and their functional roles in A431 cells. Greig, A.V., Linge, C., Healy, V., Lim, P., Clayton, E., Rustin, M.H., McGrouther, D.A., Burnstock, G. J. Invest. Dermatol. (2003) [Pubmed]
  3. Cloning and expression of a human P2U nucleotide receptor, a target for cystic fibrosis pharmacotherapy. Parr, C.E., Sullivan, D.M., Paradiso, A.M., Lazarowski, E.R., Burch, L.H., Olsen, J.C., Erb, L., Weisman, G.A., Boucher, R.C., Turner, J.T. Proc. Natl. Acad. Sci. U.S.A. (1994) [Pubmed]
  4. Cloning and functional expression of a human uridine nucleotide receptor. Communi, D., Pirotton, S., Parmentier, M., Boeynaems, J.M. J. Biol. Chem. (1995) [Pubmed]
  5. Uridine adenosine tetraphosphate: a novel endothelium- derived vasoconstrictive factor. Jankowski, V., Tölle, M., Vanholder, R., Schönfelder, G., van der Giet, M., Henning, L., Schlüter, H., Paul, M., Zidek, W., Jankowski, J. Nat. Med. (2005) [Pubmed]
  6. P2-purinergic receptors: subtype-associated signaling responses and structure. Harden, T.K., Boyer, J.L., Nicholas, R.A. Annu. Rev. Pharmacol. Toxicol. (1995) [Pubmed]
  7. ATP receptor regulation of adenylate cyclase and protein kinase C activity in cultured renal LLC-PK1 cells. Anderson, R.J., Breckon, R., Dixon, B.S. J. Clin. Invest. (1991) [Pubmed]
  8. Regulation of mucin secretion in human gallbladder epithelial cells: predominant role of calcium and protein kinase C. Dray-Charier, N., Paul, A., Combettes, L., Bouin, M., Mergey, M., Balladur, P., Capeau, J., Housset, C. Gastroenterology (1997) [Pubmed]
  9. Adenosine triphosphate activates ion permeabilities in biliary epithelial cells. McGill, J.M., Basavappa, S., Mangel, A.W., Shimokura, G.H., Middleton, J.P., Fitz, J.G. Gastroenterology (1994) [Pubmed]
  10. Uridine nucleotide selectivity of three phospholipase C-activating P2 receptors: identification of a UDP-selective, a UTP-selective, and an ATP- and UTP-specific receptor. Nicholas, R.A., Watt, W.C., Lazarowski, E.R., Li, Q., Harden, K. Mol. Pharmacol. (1996) [Pubmed]
  11. P2 receptors activated by uracil nucleotides--an update. Brunschweiger, A., Müller, C.E. Current medicinal chemistry. (2006) [Pubmed]
  12. Enzymatic synthesis of UTP gamma S, a potent hydrolysis resistant agonist of P2U-purinoceptors. Lazarowski, E.R., Watt, W.C., Stutts, M.J., Brown, H.A., Boucher, R.C., Harden, T.K. Br. J. Pharmacol. (1996) [Pubmed]
  13. Human 5-HT1B receptor stimulated inositol phospholipid hydrolysis in CHO cells: synergy with Gq-coupled receptors. Dickenson, J.M., Hill, S.J. Eur. J. Pharmacol. (1998) [Pubmed]
  14. Growth inhibition and apoptosis induced by P2Y2 receptors in human colorectal carcinoma cells: involvement of intracellular calcium and cyclic adenosine monophosphate. Höpfner, M., Maaser, K., Barthel, B., von Lampe, B., Hanski, C., Riecken, E.O., Zeitz, M., Scherübl, H. International journal of colorectal disease. (2001) [Pubmed]
  15. Purinergic receptors are part of a functional signaling system for proliferation and differentiation of human epidermal keratinocytes. Greig, A.V., Linge, C., Terenghi, G., McGrouther, D.A., Burnstock, G. J. Invest. Dermatol. (2003) [Pubmed]
  16. Human keratinocytes express multiple P2Y-receptors: evidence for functional P2Y1, P2Y2, and P2Y4 receptors. Burrell, H.E., Bowler, W.B., Gallagher, J.A., Sharpe, G.R. J. Invest. Dermatol. (2003) [Pubmed]
  17. P2X and P2Y purinergic receptors on human intestinal epithelial carcinoma cells: effects of extracellular nucleotides on apoptosis and cell proliferation. Coutinho-Silva, R., Stahl, L., Cheung, K.K., de Campos, N.E., de Oliveira Souza, C., Ojcius, D.M., Burnstock, G. Am. J. Physiol. Gastrointest. Liver Physiol. (2005) [Pubmed]
  18. Involvement of distinct receptors in the actions of extracellular uridine nucleotides. Boeynaems, J.M., Communi, D., Pirotton, S., Motte, S., Parmentier, M. Ciba Found. Symp. (1996) [Pubmed]
  19. Functional expression and photoaffinity labeling of a cloned P2U purinergic receptor. Erb, L., Lustig, K.D., Sullivan, D.M., Turner, J.T., Weisman, G.A. Proc. Natl. Acad. Sci. U.S.A. (1993) [Pubmed]
  20. Purinergic receptors are part of a signaling system for keratinocyte proliferation, differentiation, and apoptosis in human fetal epidermis. Greig, A.V., Linge, C., Cambrey, A., Burnstock, G. J. Invest. Dermatol. (2003) [Pubmed]
  21. Expression of P2Y receptors in cell lines derived from the human lung. Communi, D., Paindavoine, P., Place, G.A., Parmentier, M., Boeynaems, J.M. Br. J. Pharmacol. (1999) [Pubmed]
  22. Pharmacological profiles of cloned mammalian P2Y-receptor subtypes. von Kügelgen, I. Pharmacol. Ther. (2006) [Pubmed]
  23. Membrane-specific expression of functional purinergic receptors in normal human nasal epithelial cells. Kim, C.H., Kim, S.S., Choi, J.Y., Shin, J.H., Kim, J.Y., Namkung, W., Lee, J.G., Lee, M.G., Yoon, J.H. Am. J. Physiol. Lung Cell Mol. Physiol. (2004) [Pubmed]
  24. Agonists and antagonists for P2 receptors. Jacobson, K.A., Costanzi, S., Joshi, B.V., Besada, P., Shin, D.H., Ko, H., Ivanov, A.A., Mamedova, L. Novartis Found. Symp. (2006) [Pubmed]
  25. The P2Y2 nucleotide receptor requires interaction with alpha v integrins to access and activate G12. Liao, Z., Seye, C.I., Weisman, G.A., Erb, L. J. Cell. Sci. (2007) [Pubmed]
  26. P2Y2 nucleotide receptors enhance alpha-secretase-dependent amyloid precursor protein processing. Camden, J.M., Schrader, A.M., Camden, R.E., González, F.A., Erb, L., Seye, C.I., Weisman, G.A. J. Biol. Chem. (2005) [Pubmed]
  27. Molecular cloning and characterization of a novel orphan receptor (P2P) expressed in human pancreas that shows high structural homology to the P2U purinoceptor. Stam, N.J., Klomp, J., Van de Heuvel, N., Olijve, W. FEBS Lett. (1996) [Pubmed]
  28. Negative regulation of CFTR activity by extracellular ATP involves P2Y2 receptors in CFTR-expressing CHO cells. Marcet, B., Chappe, V., Delmas, P., Gola, M., Verrier, B. J. Membr. Biol. (2003) [Pubmed]
  29. Constitutive interaction of the P2Y2 receptor with the hematopoietic cell-specific G protein G(alpha16) and evidence for receptor oligomers. Kotevic, I., Kirschner, K.M., Porzig, H., Baltensperger, K. Cell. Signal. (2005) [Pubmed]
  30. P2 receptor expression profiles in human vascular smooth muscle and endothelial cells. Wang, L., Karlsson, L., Moses, S., Hultgårdh-Nilsson, A., Andersson, M., Borna, C., Gudbjartsson, T., Jern, S., Erlinge, D. J. Cardiovasc. Pharmacol. (2002) [Pubmed]
  31. Uridine-5'-triphosphate and adenosine triphosphate gammaS induce mucin secretion via Ca2+-dependent pathways in human nasal epithelial cells. Choi, J.Y., Namkung, W., Shin, J.H., Yoon, J.H. Acta Otolaryngol. (2003) [Pubmed]
  32. Expression and regulation of P2U-purinergic receptor in human granulosa-luteal cells. Tai, C.J., Kang, S.K., Cheng, K.W., Choi, K.C., Nathwani, P.S., Leung, P.C. J. Clin. Endocrinol. Metab. (2000) [Pubmed]
  33. Agonist-induced phosphorylation and desensitization of the P2Y2 nucleotide receptor. Flores, R.V., Hernández-Pérez, M.G., Aquino, E., Garrad, R.C., Weisman, G.A., Gonzalez, F.A. Mol. Cell. Biochem. (2005) [Pubmed]
  34. Differences in the sensitivity to purinergic stimulation of myelinating and non-myelinating Schwann cells in peripheral human and rat nerve. Mayer, C., Quasthoff, S., Grafe, P. Glia (1998) [Pubmed]
  35. Transient up-regulation of P2Y2 nucleotide receptor mRNA expression is an immediate early gene response in activated thymocytes. Koshiba, M., Apasov, S., Sverdlov, V., Chen, P., Erb, L., Turner, J.T., Weisman, G.A., Sitkovsky, M.V. Proc. Natl. Acad. Sci. U.S.A. (1997) [Pubmed]
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